##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n01_35008-1.341000000357b4.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2463384 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.67495485884458 33.0 33.0 33.0 33.0 33.0 2 32.708604099076716 33.0 33.0 33.0 33.0 33.0 3 32.74373991225079 33.0 33.0 33.0 33.0 33.0 4 36.6718615530506 37.0 37.0 37.0 37.0 37.0 5 36.66930571928697 37.0 37.0 37.0 37.0 37.0 6 36.52941400934649 37.0 37.0 37.0 37.0 37.0 7 36.46358992345489 37.0 37.0 37.0 37.0 37.0 8 36.39147205632577 37.0 37.0 37.0 37.0 37.0 9 36.41066435440029 37.0 37.0 37.0 37.0 37.0 10-11 36.36708101538372 37.0 37.0 37.0 37.0 37.0 12-13 36.340328994586315 37.0 37.0 37.0 37.0 37.0 14-15 38.13854153473433 40.0 37.0 40.0 37.0 40.0 16-17 38.12298975717955 40.0 37.0 40.0 37.0 40.0 18-19 38.13882955316751 40.0 37.0 40.0 37.0 40.0 20-21 38.091212738249496 40.0 37.0 40.0 37.0 40.0 22-23 37.993144795939244 40.0 37.0 40.0 33.0 40.0 24-25 37.957200542018626 40.0 37.0 40.0 33.0 40.0 26-27 37.84301290420008 40.0 37.0 40.0 33.0 40.0 28-29 37.72320982031222 40.0 37.0 40.0 33.0 40.0 30-31 37.69010292345814 40.0 37.0 40.0 33.0 40.0 32-33 37.576402014464655 40.0 37.0 40.0 33.0 40.0 34-35 37.48503481389828 40.0 37.0 40.0 33.0 40.0 36-37 37.40239585058602 40.0 37.0 40.0 33.0 40.0 38-39 37.31530001006745 40.0 37.0 40.0 33.0 40.0 40-41 37.22729119779945 40.0 37.0 40.0 33.0 40.0 42-43 37.081021878846336 38.5 37.0 40.0 33.0 40.0 44-45 36.959876332719546 37.0 37.0 40.0 33.0 40.0 46-47 36.90967039649523 37.0 37.0 40.0 33.0 40.0 48-49 36.687258462342854 37.0 37.0 40.0 33.0 40.0 50-51 36.50810328393787 37.0 37.0 40.0 33.0 40.0 52-53 36.365862975484134 37.0 37.0 40.0 33.0 40.0 54-55 36.20439221006551 37.0 37.0 40.0 33.0 40.0 56-57 36.03769509747566 37.0 37.0 40.0 33.0 40.0 58-59 35.75430992488382 37.0 37.0 40.0 27.0 40.0 60-61 35.49275041974779 37.0 33.0 40.0 27.0 40.0 62-63 35.23019837751646 37.0 33.0 40.0 27.0 40.0 64-65 34.96974548020122 37.0 33.0 37.0 27.0 40.0 66-67 34.60342256830441 37.0 33.0 37.0 27.0 40.0 68-69 34.32077053354247 37.0 33.0 37.0 27.0 40.0 70-71 33.96107854073908 37.0 33.0 37.0 27.0 38.5 72-73 33.564560986025725 37.0 33.0 37.0 27.0 37.0 74-75 33.15205769786603 37.0 33.0 37.0 27.0 37.0 76-77 30.600590082585583 33.0 30.0 35.0 24.5 37.0 78-79 32.9097402191457 35.0 33.0 37.0 27.0 37.0 80-81 33.30248065263069 37.0 33.0 37.0 27.0 37.0 82-83 33.2317503077068 37.0 33.0 37.0 27.0 37.0 84-85 33.05185793201547 37.0 33.0 37.0 27.0 37.0 86-87 32.94330664646681 37.0 33.0 37.0 27.0 37.0 88-89 32.82642860390422 35.0 33.0 37.0 27.0 37.0 90-91 32.671650664289444 33.0 33.0 37.0 27.0 37.0 92-93 32.51990716835053 33.0 33.0 37.0 27.0 37.0 94-95 32.39232799271247 33.0 33.0 37.0 24.5 37.0 96-97 32.22216654001163 33.0 33.0 37.0 22.0 37.0 98-99 32.052132757215276 33.0 33.0 37.0 22.0 37.0 100-101 30.385229424239178 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.118553439859447 2306 2 0.144484548553919 2306 3 0.14751917943026172 2306 4 0.1973875752388068 2306 5 0.19658007129535093 2306 6 0.2388436099964224 2306 7 0.27376414172522345 2306 8 0.2563471092536673 2306 9 0.2280090230874734 2306 10-11 0.21457700334717345 2306 12-13 0.22620255732751815 2306 14-15 0.3887375345786097 2306 16-17 0.3935523984855891 2306 18-19 0.34958288860901376 2306 20-21 0.4291936997388106 2306 22-23 0.4291633580435956 2306 24-25 0.40746817300365734 2306 26-27 0.5117573191689857 2306 28-29 0.5236756908797986 2306 30-31 0.46887162734916643 2306 32-33 0.542115452653853 2306 34-35 0.6698740358100466 2306 36-37 0.5279710552539569 2306 38-39 0.651996040189168 2306 40-41 0.6429403017074264 2306 42-43 0.6374179866418572 2306 44-45 0.4863419843361214 2306 46-47 0.6773250887075619 2306 48-49 0.7001305147696115 2306 50-51 0.7093933063243227 2306 52-53 0.7065974489061091 2306 54-55 0.6041632139584863 2306 56-57 0.655832952167728 2306 58-59 0.6927355063767138 2306 60-61 0.6506443031346549 2306 62-63 0.723151573704726 2306 64-65 0.6580046140699807 2306 66-67 0.6444339968143638 2306 68-69 0.7331281409975432 2306 70-71 0.6649446732926023 2306 72-73 0.6170336649406707 2306 74-75 0.6241979707708012 2306 76-77 0.5573759195830377 2306 78-79 0.7304911434688464 2306 80-81 0.7208858730478056 2306 82-83 0.704986071155659 2306 84-85 0.7873994312157109 2306 86-87 0.7260038425441664 2306 88-89 0.594671552028494 2306 90-91 0.7650023163435336 2306 92-93 0.907759277278025 2306 94-95 0.7192829885156726 2306 96-97 0.842558255581892 2306 98-99 0.9894019508438063 2306 100-101 0.8310297245743641 2307 1 0.1369429297296847 2307 2 0.13569148696606703 2307 3 0.15574384154685106 2307 4 0.17370633215158904 2307 5 0.1806972581630788 2307 6 0.23667225387674762 2307 7 0.1600030475495018 2307 8 0.20805203790024507 2307 9 0.19366307364184365 2307 10-11 0.1998233007924739 2307 12-13 0.245358455787148 2307 14-15 0.5133736186380631 2307 16-17 0.5601889411492706 2307 18-19 0.5128395181377314 2307 20-21 0.5289345883519374 2307 22-23 0.517097885546363 2307 24-25 0.5976199170485543 2307 26-27 0.5797534884748856 2307 28-29 0.5810281956681607 2307 30-31 0.5925020193208965 2307 32-33 0.5755355611002599 2307 34-35 0.49867883227069854 2307 36-37 0.5711754848312296 2307 38-39 0.6749614560352981 2307 40-41 0.5834730919094824 2307 42-43 0.607265837946187 2307 44-45 0.5952708737045924 2307 46-47 0.485978276006918 2307 48-49 0.4349729707301222 2307 50-51 0.425705723708127 2307 52-53 0.28454627406998867 2307 54-55 0.35111417028317504 2307 56-57 0.3523722437590848 2307 58-59 0.49510851347159246 2307 60-61 0.509834462027527 2307 62-63 0.6577730903358727 2307 64-65 0.5564999875626526 2307 66-67 0.5069974595460209 2307 68-69 0.6433226431421915 2307 70-71 0.5792123902528559 2307 72-73 0.2260183961176594 2307 74-75 0.2522474190968964 2307 76-77 0.34977361037588395 2307 78-79 0.404866362654694 2307 80-81 0.4327978480134149 2307 82-83 0.37620096769993694 2307 84-85 0.5263713484511356 2307 86-87 0.47430093715151855 2307 88-89 0.5623425169857441 2307 90-91 0.553156029688914 2307 92-93 0.5393610199740735 2307 94-95 0.17718163698204137 2307 96-97 0.48970749079190057 2307 98-99 0.5696657831565162 2307 100-101 0.43932317732463844 2201 1 0.12947390595782338 2201 2 0.09939839334420242 2201 3 0.10530755825249827 2201 4 0.1595116074590308 2201 5 0.07920982547276623 2201 6 0.18342769278883964 2201 7 0.17823248196661723 2201 8 0.23313994572821173 2201 9 0.24962655130623546 2201 10-11 0.2534590710722071 2201 12-13 0.24157520141917388 2201 14-15 0.49962658856572517 2201 16-17 0.4442796996508349 2201 18-19 0.4562521620898963 2201 20-21 0.40239186975523467 2201 22-23 0.47595580349601363 2201 24-25 0.4390683659079144 2201 26-27 0.5311767816439001 2201 28-29 0.5528589759870712 2201 30-31 0.504767328712532 2201 32-33 0.5399557070789172 2201 34-35 0.5812967046664426 2201 36-37 0.6019421456843475 2201 38-39 0.6159449726987489 2201 40-41 0.6027977089700087 2201 42-43 0.5471442711495627 2201 44-45 0.5827827705512902 2201 46-47 0.6173575435445002 2201 48-49 0.5836938994571881 2201 50-51 0.7083496682544066 2201 52-53 0.4394762841815165 2201 54-55 0.6110328211115856 2201 56-57 0.6066563144366981 2201 58-59 0.5569435562190037 2201 60-61 0.426929592882054 2201 62-63 0.5811053548887415 2201 64-65 0.6121487969640711 2201 66-67 0.5493422369540824 2201 68-69 0.6476116007613584 2201 70-71 0.544778619227273 2201 72-73 0.440445333041076 2201 74-75 0.39772798823645417 2201 76-77 0.19024289385378523 2201 78-79 0.4791195681710221 2201 80-81 0.5496099678315716 2201 82-83 0.5544630138839466 2201 84-85 0.6104371518173579 2201 86-87 0.564371585825107 2201 88-89 0.4236268712328979 2201 90-91 0.6000386814017986 2201 92-93 0.5368305997148681 2201 94-95 0.40881936660800733 2201 96-97 0.4477451641609136 2201 98-99 0.46747082287529196 2201 100-101 0.6527176367768597 1101 1 0.07832794817634436 1101 2 0.02557307679094123 1101 3 0.06106789271202473 1101 4 0.09061069066425631 1101 5 0.05126635472727514 1101 6 0.09254753840534136 1101 7 0.06915774099717709 1101 8 0.09009755164206013 1101 9 0.13081149035073025 1101 10-11 0.15447007175306027 1101 12-13 0.22678094855053388 1101 14-15 0.3222293693528613 1101 16-17 0.25983987510220885 1101 18-19 0.25588047696030003 1101 20-21 0.31130831625127797 1101 22-23 0.3776665685193521 1101 24-25 0.26132251346576396 1101 26-27 0.2751326162011978 1101 28-29 0.2155711355433283 1101 30-31 0.4013406601617433 1101 32-33 0.2957354536650243 1101 34-35 0.3009759620955066 1101 36-37 0.4263023662403569 1101 38-39 0.4052320480763214 1101 40-41 0.48162072033324677 1101 42-43 0.4007725546179941 1101 44-45 0.38595950797230216 1101 46-47 0.4741709858712113 1101 48-49 0.400652533071856 1101 50-51 0.42494439586374 1101 52-53 0.4407541302347866 1101 54-55 0.49581471302315094 1101 56-57 0.5731500561669236 1101 58-59 0.3963360422986497 1101 60-61 0.31777206490218646 1101 62-63 0.3565195397292982 1101 64-65 0.2614640912932984 1101 66-67 0.3957973114409796 1101 68-69 0.4087057738404596 1101 70-71 0.39409377082028385 1101 72-73 0.268603764541254 1101 74-75 0.23295043550612604 1101 76-77 0.17631469302962444 1101 78-79 0.39104096904142693 1101 80-81 0.48839004413835596 1101 82-83 0.4238351029570637 1101 84-85 0.5300406163110125 1101 86-87 0.5305124122393039 1101 88-89 0.5045201288018433 1101 90-91 0.6351610705778228 1101 92-93 0.6375431389494892 1101 94-95 0.4413692921710677 1101 96-97 0.49713760741969537 1101 98-99 0.67884782778399 1101 100-101 0.34889109269206386 2304 1 0.0914644785180414 2304 2 0.05419577351898397 2304 3 0.03594023206184005 2304 4 0.10566889801476975 2304 5 0.1528539418123529 2304 6 0.06331030771416835 2304 7 0.1910300942170764 2304 8 0.20662656896489295 2304 9 0.11309984749362201 2304 10-11 0.08112800474447113 2304 12-13 0.14384112276766814 2304 14-15 0.2916276137588554 2304 16-17 0.33470284096346603 2304 18-19 0.2915391065875923 2304 20-21 0.2780921627104007 2304 22-23 0.3623887888773183 2304 24-25 0.3151556438932772 2304 26-27 0.3269180085029504 2304 28-29 0.3173412707586962 2304 30-31 0.24570674611954502 2304 32-33 0.25522677077215405 2304 34-35 0.3358241988282131 2304 36-37 0.2894219169213983 2304 38-39 0.26690754461395017 2304 40-41 0.35389978004929645 2304 42-43 0.3303709442571403 2304 44-45 0.3546489242522881 2304 46-47 0.3071783723591608 2304 48-49 0.3781114183560135 2304 50-51 0.4255344334784965 2304 52-53 0.3083929775507315 2304 54-55 0.32548366109401883 2304 56-57 0.4145404510033117 2304 58-59 0.3244984313883563 2304 60-61 0.44391863941784493 2304 62-63 0.5389666645291342 2304 64-65 0.41952068300337686 2304 66-67 0.3454097769727227 2304 68-69 0.5184099295396933 2304 70-71 0.40591812462003674 2304 72-73 0.41333781025971206 2304 74-75 0.47417235363060684 2304 76-77 0.3145114575429844 2304 78-79 0.6075964066267474 2304 80-81 0.5879072982923361 2304 82-83 0.5970191405548206 2304 84-85 0.5784334321472429 2304 86-87 0.49166523052740274 2304 88-89 0.5674172436912812 2304 90-91 0.5090218785233205 2304 92-93 0.4947341259040101 2304 94-95 0.4543714840908919 2304 96-97 0.4741488471049422 2304 98-99 0.6691411217799939 2304 100-101 0.6783934879651383 2202 1 0.07683783008027945 2202 2 0.09450819256962006 2202 3 0.09417544273001965 2202 4 0.08825058181184176 2202 5 0.06380095369001282 2202 6 0.12554763420789072 2202 7 0.050511198045271044 2202 8 0.055195645689096295 2202 9 0.0872226231253066 2202 10-11 0.1310301645520724 2202 12-13 0.11197453444010819 2202 14-15 0.22714274571966797 2202 16-17 0.05151333857165241 2202 18-19 0.16867684992740806 2202 20-21 0.14259678291846 2202 22-23 0.10201931869995207 2202 24-25 0.023361916797107085 2202 26-27 0.13983222341072832 2202 28-29 0.015837699675373074 2202 30-31 -0.018733470300617228 2202 32-33 0.2136669729640559 2202 34-35 0.14246092756773976 2202 36-37 0.1910714950326451 2202 38-39 0.16833239995651894 2202 40-41 0.020768362425258147 2202 42-43 0.054546657375297514 2202 44-45 0.09740998452527094 2202 46-47 0.2663351137702108 2202 48-49 0.29681680596931415 2202 50-51 0.12519956725967774 2202 52-53 0.29310168118491475 2202 54-55 0.17172244221782051 2202 56-57 0.27226530663881476 2202 58-59 0.1747492671938531 2202 60-61 0.10270180538083906 2202 62-63 0.25409236908941324 2202 64-65 0.18413006921728226 2202 66-67 0.2913103859002817 2202 68-69 0.31740723495885703 2202 70-71 0.19311037055462066 2202 72-73 0.20630944873978763 2202 74-75 0.1475651150465822 2202 76-77 0.15911942930073053 2202 78-79 0.2514702421589661 2202 80-81 0.21376878788206 2202 82-83 0.35649432291713623 2202 84-85 0.2687372096239997 2202 86-87 0.28944664767019646 2202 88-89 0.3201519984275123 2202 90-91 0.3628015313622157 2202 92-93 0.3087610742232201 2202 94-95 0.12183346848919285 2202 96-97 0.32450723179600516 2202 98-99 0.3684462597292182 2202 100-101 0.4769033709544601 1102 1 0.0924287885265116 1102 2 0.08842574385320034 1102 3 0.09399044011890823 1102 4 0.10125423580856108 1102 5 0.11377976035291937 1102 6 0.058497457908991635 1102 7 0.09478887993913787 1102 8 0.15349604153223595 1102 9 0.13284372060450522 1102 10-11 0.156325368613075 1102 12-13 0.14609327020876606 1102 14-15 0.16994083605558075 1102 16-17 0.1454294847721158 1102 18-19 0.0851793950793649 1102 20-21 0.15235410945118844 1102 22-23 0.21711182390851747 1102 24-25 0.2884062588786449 1102 26-27 0.37960907279284584 1102 28-29 0.26196962292973325 1102 30-31 0.186730468631346 1102 32-33 0.2370649245089851 1102 34-35 0.21910879734463862 1102 36-37 0.26417504263335445 1102 38-39 0.21827959224916782 1102 40-41 0.2458878530357893 1102 42-43 0.22351200100008128 1102 44-45 0.43694031987958226 1102 46-47 0.2579055907881269 1102 48-49 0.2595239483971241 1102 50-51 0.15749267072141748 1102 52-53 0.26263499063183815 1102 54-55 0.20485254588952984 1102 56-57 0.23890785271188264 1102 58-59 0.3044308285222215 1102 60-61 0.06878505031102122 1102 62-63 0.13532108305586377 1102 64-65 0.17948822100216688 1102 66-67 0.20521020971846582 1102 68-69 0.307239897365001 1102 70-71 0.0926843961880266 1102 72-73 -0.04128610276705302 1102 74-75 -0.12659470379136195 1102 76-77 -0.07727175520776086 1102 78-79 0.2421590295175946 1102 80-81 0.21029393926025364 1102 82-83 0.306557473597465 1102 84-85 0.2759997330127746 1102 86-87 0.262156615876286 1102 88-89 0.12651320002337485 1102 90-91 0.3195118961139869 1102 92-93 0.30068282202172014 1102 94-95 0.02551989921117581 1102 96-97 0.19725783032238553 1102 98-99 0.33516764657748155 1102 100-101 0.15576245126511168 2305 1 0.07275953590764317 2305 2 0.04258396375859519 2305 3 0.049075975460738164 2305 4 0.04549939519273494 2305 5 0.0653165589792053 2305 6 0.06984573652486148 2305 7 -0.013489092370114975 2305 8 0.012483029863275874 2305 9 0.1116719683293681 2305 10-11 0.1032603278199673 2305 12-13 0.10863078699259177 2305 14-15 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1213 98-99 0.020008158328217007 1213 100-101 0.10945846552804639 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 3.0 9 23.0 10 36.0 11 21.0 12 34.0 13 72.0 14 208.0 15 522.0 16 955.0 17 1603.0 18 2474.0 19 3451.0 20 4502.0 21 6004.0 22 7824.0 23 10031.0 24 12770.0 25 15826.0 26 20503.0 27 26761.0 28 37786.0 29 49859.0 30 52322.0 31 60380.0 32 80518.0 33 114015.0 34 173428.0 35 295122.0 36 557667.0 37 769128.0 38 159483.0 39 52.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 34.685354335064275 25.560371773914408 24.668682184226657 15.085591706794666 2 28.75479421803503 23.402928654241485 34.0294083261075 13.812868801615988 3 36.76755227767981 19.504064327770255 26.896253284100247 16.83213011044969 4 27.908802094584388 19.278874030954242 34.710557218568646 18.101766655892725 5 32.15686226751493 18.200978816132604 32.76078759949728 16.881371316855187 6 25.78310973847358 19.490180986805143 37.965741435358844 16.760967839362436 7 31.456524845497086 20.90291241641579 29.621122813170825 18.019439924916295 8 27.504359856197812 28.17112557360119 26.628207376519452 17.696307193681537 9 30.289999447913928 24.557519249942356 25.985676613958685 19.16680468818503 10-11 24.220097232100233 28.76212153687773 29.065180256102984 17.952600974919054 12-13 23.638539505006122 26.932910175595847 29.011047404708318 20.417502914689713 14-15 22.916910234052022 27.468311883165597 29.790320956862594 19.824456925919794 16-17 22.607518762807587 27.64546656144556 30.63223192161677 19.11478275413009 18-19 20.890409290634345 27.942212825933755 31.491882710937475 19.675495172494422 20-21 21.76903803873046 28.13540235708278 30.990154194392755 19.105405409794006 22-23 21.103632239228638 29.493594989656508 30.314904213066253 19.087868558048605 24-25 21.467420426535206 27.431492613413095 31.177904053935563 19.92318290611614 26-27 21.350954621772324 27.636576351880176 30.215447530713845 20.79702149563365 28-29 21.382090652533265 28.746127278572892 30.049456357595894 19.822325711297957 30-31 20.147447576179758 28.750186734995438 30.519703789583758 20.582661899241046 32-33 19.94041529862985 29.582355004335497 30.367250903635 20.109978793399648 34-35 21.02731445848475 28.918877446634383 30.65805006446417 19.395758030416697 36-37 20.154105084712736 29.40193246363539 31.281947922045443 19.162014529606427 38-39 20.749992692978438 29.748508555710355 29.715789336944624 19.78570941436658 40-41 20.589075840388666 28.584378237416498 31.121741474329625 19.70480444786521 42-43 20.59138973054952 29.463453525719093 30.780422378321852 19.164734365409533 44-45 20.084201245116475 28.908667913731676 31.232848796614736 19.77428204453711 46-47 21.05083580216142 28.814146567605025 30.332080042802943 19.802937587430613 48-49 20.666548937699048 30.151951560619256 30.063187978654522 19.118311523027177 50-51 21.330342592222355 29.39036417638282 29.648099430843107 19.63119380055172 52-53 20.201765555171924 28.952043890635526 29.43399811107397 21.412192443118585 54-55 21.41262478056322 29.116804513292877 29.809278785802096 19.66129192034181 56-57 20.69406665961566 29.027970095884886 29.422975303952832 20.85498794054662 58-59 20.543016095192755 28.811399907227052 30.242336335428 20.40324766215219 60-61 20.758768543259553 29.19505345281562 30.397853443397338 19.648324560527488 62-63 20.215205363977056 29.296500680077692 29.960543112216577 20.527750843728683 64-65 21.164908220797873 28.988951986561716 29.790275515227176 20.05586427741323 66-67 20.837100884845082 30.221907599809494 28.898659597542263 20.042331917803157 68-69 21.030270779704484 30.291975767458883 28.748094333849767 19.929659118986862 70-71 20.5216862042174 31.15895731785677 28.800828643082742 19.518527834843084 72-73 20.192718399298393 31.433237782794404 28.699835967063485 19.674207850843715 74-75 20.86549846273284 30.575719949391182 28.561985242884962 19.99679634499101 76-77 21.29056789315434 29.612625811804484 29.095401478184023 20.001404816857153 78-79 21.102266464625753 29.45740242104013 29.708762695642555 19.73156841869156 80-81 20.31894498285313 30.09514762344072 29.469213273778532 20.116694119927615 82-83 19.543108622373992 29.969397545511434 29.655233726068552 20.832260106046018 84-85 19.6642029697112 29.495269259350298 30.05902439549166 20.78150337544683 86-87 19.69238814645009 29.676993952150305 30.747644635489717 19.88297326590989 88-89 19.924928186441193 30.17578335143475 30.26275134745582 19.636537114668236 90-91 20.324610057235525 29.629661209467645 29.706481421141433 20.339247312155397 92-93 19.68472333964504 30.45351903428683 29.593125459192258 20.26863216687587 94-95 19.29086917887797 30.934524170835676 29.562573477419964 20.2120331728664 96-97 19.832534933219424 31.401364764771756 28.996338376558462 19.769761925450354 98-99 20.31618126578084 30.402113505923325 28.698838400122145 20.58286682817369 100-101 20.198217785803394 30.307669976725045 28.349771970372895 21.14434026709867 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 176.0 1 116.0 2 104.0 3 211.5 4 208.0 5 121.0 6 84.5 7 81.0 8 92.0 9 89.5 10 84.0 11 81.5 12 85.0 13 102.0 14 113.5 15 108.0 16 106.5 17 130.0 18 176.0 19 242.0 20 350.5 21 497.5 22 737.0 23 1171.0 24 1745.5 25 2575.5 26 3827.0 27 5487.5 28 7372.0 29 9443.5 30 11719.0 31 14408.5 32 20065.0 33 30860.5 34 47268.5 35 71550.0 36 106342.5 37 148899.0 38 190383.0 39 208127.0 40 205434.5 41 186167.0 42 159245.5 43 149129.5 44 140281.0 45 129917.0 46 127538.5 47 113262.5 48 84321.0 49 63336.0 50 50394.0 51 40879.5 52 34031.5 53 26864.0 54 19955.0 55 14083.5 56 9646.5 57 6564.0 58 4679.0 59 3413.5 60 2510.0 61 1860.5 62 1322.0 63 923.0 64 655.5 65 457.5 66 324.5 67 228.5 68 179.0 69 141.5 70 102.0 71 72.0 72 57.0 73 43.5 74 30.5 75 22.5 76 16.5 77 12.0 78 10.0 79 7.5 80 3.5 81 2.0 82 1.5 83 1.5 84 1.0 85 0.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19456974633268706 2 0.0 3 0.0 4 0.026467655875007712 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 8.11891284509439E-5 48-49 0.004018861858321723 50-51 1.4208097478915184E-4 52-53 0.0032272678559250203 54-55 0.010554586698622708 56-57 0.015365042559341133 58-59 0.01548682625201755 60-61 0.0036535107802924756 62-63 0.002537160264091997 64-65 0.011021424187215636 66-67 0.0189576614932954 68-69 0.026893898799375166 70-71 0.02283444237682797 72-73 0.01883587780061899 74-75 0.023220090736969956 76-77 0.017435365334840205 78-79 0.007570886228050519 80-81 0.004323321090012763 82-83 7.91594002396703E-4 84-85 0.0032678624201504924 86-87 0.01796309466977134 88-89 0.016603176768218028 90-91 0.019911633752593992 92-93 0.02027698483062324 94-95 0.018977958775408137 96-97 0.022002253810205798 98-99 0.030283544912202077 100-101 0.01771952728441851 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2463384.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.08716653004473 #Duplication Level Percentage of deduplicated Percentage of total 1 87.77781115289413 35.187637333288855 2 4.912992212806044 3.9389587399113766 3 2.1627150804676316 2.6009135876323515 4 1.0498105225359866 1.6833571696757348 5 0.7300121807894457 1.4632059930133816 6 0.5079743906908191 1.221795239557252 7 0.31994112481043174 0.8977873205059923 8 0.29963760967414427 0.9609298206137573 9 0.15331411453013427 0.5531335596518259 >10 1.6352519659808846 13.022670771183842 >50 0.21296151053532184 6.004264004455158 >100 0.19686805250530537 17.194638954114268 >500 0.032540883971894134 8.778368619252308 >1k 0.00796638253819018 4.866931413292681 >5k 1.0140763485441052E-4 0.20894843993549359 >10k+ 1.0140763475989365E-4 1.4164590339156542 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT 34844 1.4144769958723447 TruSeq Adapter, Index 2 (97% over 37bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 5140 0.20865606011892585 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 4203 0.1706189534396586 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 4171 0.1693199273844435 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 3929 0.1594960428418793 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 3369 0.136763086875615 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 3182 0.12917190336545176 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 2966 0.12040347749274981 No Hit TTGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC 2759 0.11200040269807711 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 9.742695414113268E-4 0.0 0.0 2 0.0010554586698622707 0.0 0.0 3 0.0011772423625386866 0.0 0.0 4 0.0012990260552151025 0.0 0.0 5 0.0013802151836660463 0.0 0.0 6 0.0014614043121169904 0.0 0.0 7 0.0018267553901462378 0.0 0.0 8 0.0021109173397245414 0.0 0.0 9 0.002354484725077373 0.0 0.0 10-11 0.0036941053445179476 0.0 0.0 12-13 0.005013428681845787 0.0 0.0 14-15 0.006454535711850041 0.0 0.0 16-17 0.008260993819883542 0.0 0.0 18-19 0.010412505723833555 0.0 0.0 20-21 0.012787287731023665 0.0 0.0 22-23 0.015486826252017551 0.0 0.0 24-25 0.018592310415266156 0.0 0.0 26-27 0.02220522663133316 0.0 0.0 28-29 0.0253310080766945 0.0 0.0 30-31 0.038605430578423826 0.0 0.0 32-33 0.043131724489563955 0.0 0.0 34-35 0.04731296460478756 0.0 0.0 36-37 0.0515145020021239 0.0 0.0 38-39 0.056264066016504126 0.0 0.0 40-41 0.061663143058491895 0.0 0.0 42-43 0.0668592472793523 0.0 0.0 44-45 0.07231921616767828 0.0 0.0 46-47 0.07775888777389153 0.0 0.0 48-49 0.08443669358898166 0.0 0.0 50-51 0.09174371514956661 0.0 0.0 52-53 0.09905073671015156 0.0 0.0 54-55 0.10690578488778038 0.0 0.0 56-57 0.11571480532470779 0.0 0.0 58-59 0.1255183925851593 0.0 0.0 60-61 0.13615416841223293 0.0 0.0 62-63 0.14794688931973254 0.0 0.0 64-65 0.15973961022723213 0.0 0.0 66-67 0.1717353039558591 0.0 0.0 68-69 0.18574042861364692 0.0 0.0 70-71 0.20189706517538475 0.0 0.0 72-73 0.21866262020050467 0.0 0.0 74-75 0.23753909256534914 0.0 0.0 76-77 0.2581611311918889 0.0 0.0 78-79 0.2802851686947711 0.0 0.0 80-81 0.3037082322528684 0.0 4.059456422547195E-5 82-83 0.3318402652611205 0.0 4.059456422547195E-5 84-85 0.3636867008960032 0.0 4.059456422547195E-5 86-87 0.39709602725356663 0.0 4.059456422547195E-5 88-89 0.43562026870353954 0.0 4.059456422547195E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGACGG 20 0.002059928 71.37551 1 TCGGAAG 5385 0.0 67.30081 3 CGGAAGA 5420 0.0 67.04148 4 GATCGGA 5585 0.0 65.09209 1 ATCGGAA 5605 0.0 64.65921 2 GAAGAGC 5710 0.0 63.378014 6 CGCGCGG 25 0.004987092 57.10041 1 TCGACGG 25 0.0050225123 56.9984 2 GGAAGAG 6695 0.0 54.408222 5 CGACTCG 35 2.7644352E-4 54.381344 1 AGACGGC 45 1.5007638E-5 52.87075 1 TTGTGTT 26765 0.0 48.659267 1 GCCGTCT 4185 0.0 43.130333 50-51 ATGCCGT 4150 0.0 42.920486 48-49 TGCCGTC 4140 0.0 42.796 50-51 CGTATGC 4135 0.0 42.727673 46-47 TCTCGTA 4200 0.0 42.631386 42-43 TCGTATG 4200 0.0 42.631386 44-45 TATGCCG 4135 0.0 42.559265 48-49 CGTCTTC 4245 0.0 42.41011 52-53 >>END_MODULE