FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n01_35008-1.341000000357b4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n01_35008-1.341000000357b4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2463384
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT348441.4144769958723447TruSeq Adapter, Index 2 (97% over 37bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC51400.20865606011892585No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC42030.1706189534396586No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG41710.1693199273844435No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA39290.1594960428418793No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA33690.136763086875615No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA31820.12917190336545176No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG29660.12040347749274981No Hit
TTGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC27590.11200040269807711No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACGG200.00205992871.375511
TCGGAAG53850.067.300813
CGGAAGA54200.067.041484
GATCGGA55850.065.092091
ATCGGAA56050.064.659212
GAAGAGC57100.063.3780146
CGCGCGG250.00498709257.100411
TCGACGG250.005022512356.99842
GGAAGAG66950.054.4082225
CGACTCG352.7644352E-454.3813441
AGACGGC451.5007638E-552.870751
TTGTGTT267650.048.6592671
GCCGTCT41850.043.13033350-51
ATGCCGT41500.042.92048648-49
TGCCGTC41400.042.79650-51
CGTATGC41350.042.72767346-47
TCTCGTA42000.042.63138642-43
TCGTATG42000.042.63138644-45
TATGCCG41350.042.55926548-49
CGTCTTC42450.042.4101152-53