##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n01_35005-1.3410000003578e.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2673689 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.694626039154144 33.0 33.0 33.0 33.0 33.0 2 32.724788859138066 33.0 33.0 33.0 33.0 33.0 3 32.75373426004296 33.0 33.0 33.0 33.0 33.0 4 36.68548174451105 37.0 37.0 37.0 37.0 37.0 5 36.674562374307556 37.0 37.0 37.0 37.0 37.0 6 36.5513842485046 37.0 37.0 37.0 37.0 37.0 7 36.49886505124567 37.0 37.0 37.0 37.0 37.0 8 36.422069657316165 37.0 37.0 37.0 37.0 37.0 9 36.431095015164445 37.0 37.0 37.0 37.0 37.0 10-11 36.38623714276417 37.0 37.0 37.0 37.0 37.0 12-13 36.369360834412674 37.0 37.0 37.0 37.0 37.0 14-15 38.19300300072297 40.0 37.0 40.0 37.0 40.0 16-17 38.18240790159214 40.0 37.0 40.0 37.0 40.0 18-19 38.1932537778328 40.0 37.0 40.0 37.0 40.0 20-21 38.12533226564496 40.0 37.0 40.0 37.0 40.0 22-23 38.02778221401218 40.0 37.0 40.0 35.0 40.0 24-25 37.995935204131825 40.0 37.0 40.0 35.0 40.0 26-27 37.89809977899449 40.0 37.0 40.0 33.0 40.0 28-29 37.78255754502487 40.0 37.0 40.0 33.0 40.0 30-31 37.74277281314319 40.0 37.0 40.0 33.0 40.0 32-33 37.62255726077341 40.0 37.0 40.0 33.0 40.0 34-35 37.53422050956563 40.0 37.0 40.0 33.0 40.0 36-37 37.453694689247705 40.0 37.0 40.0 33.0 40.0 38-39 37.372232522181896 40.0 37.0 40.0 33.0 40.0 40-41 37.28546906539991 40.0 37.0 40.0 33.0 40.0 42-43 37.14166326001266 40.0 37.0 40.0 33.0 40.0 44-45 37.00928698139537 37.0 37.0 40.0 33.0 40.0 46-47 36.948214433316664 37.0 37.0 40.0 33.0 40.0 48-49 36.73780533188415 37.0 37.0 40.0 33.0 40.0 50-51 36.56893565407196 37.0 37.0 40.0 33.0 40.0 52-53 36.42384866003488 37.0 37.0 40.0 33.0 40.0 54-55 36.25753126111526 37.0 37.0 40.0 33.0 40.0 56-57 36.08022062401424 37.0 37.0 40.0 33.0 40.0 58-59 35.81605788855772 37.0 37.0 40.0 27.0 40.0 60-61 35.55351875255499 37.0 35.0 40.0 27.0 40.0 62-63 35.26653118593823 37.0 33.0 40.0 27.0 40.0 64-65 35.00745318546772 37.0 33.0 37.0 27.0 40.0 66-67 34.681498109914806 37.0 33.0 37.0 27.0 40.0 68-69 34.386912052972505 37.0 33.0 37.0 27.0 40.0 70-71 34.05103379637647 37.0 33.0 37.0 27.0 40.0 72-73 33.71596359935654 37.0 33.0 37.0 27.0 37.0 74-75 33.36240752009677 37.0 33.0 37.0 27.0 37.0 76-77 30.749979709682016 33.0 30.0 35.0 24.5 37.0 78-79 33.09545145302988 35.0 33.0 37.0 27.0 37.0 80-81 33.48587550758521 37.0 33.0 37.0 27.0 37.0 82-83 33.41211337593864 37.0 33.0 37.0 27.0 37.0 84-85 33.23691031380239 37.0 33.0 37.0 27.0 37.0 86-87 33.13069470682641 37.0 33.0 37.0 27.0 37.0 88-89 32.999110405136875 37.0 33.0 37.0 27.0 37.0 90-91 32.86496933637383 33.0 33.0 37.0 27.0 37.0 92-93 32.73403488588239 33.0 33.0 37.0 27.0 37.0 94-95 32.60473787340263 33.0 33.0 37.0 27.0 37.0 96-97 32.42659692282835 33.0 33.0 37.0 27.0 37.0 98-99 32.25710357487351 33.0 33.0 37.0 22.0 37.0 100-101 30.553391587428457 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.17294935515800347 2306 2 0.1319761253649645 2306 3 0.13905401725516953 2306 4 0.21023196507962894 2306 5 0.1197223220302206 2306 6 0.21180673686642137 2306 7 0.2002104443683166 2306 8 0.24980483170067203 2306 9 0.21559562243749042 2306 10-11 0.2571467864586623 2306 12-13 0.2514679577754251 2306 14-15 0.4797182962296631 2306 16-17 0.5465089860829195 2306 18-19 0.44672709231969066 2306 20-21 0.5002640763926891 2306 22-23 0.5100788008703105 2306 24-25 0.40313215685996084 2306 26-27 0.5297563590981369 2306 28-29 0.3923623981511355 2306 30-31 0.4524392654029583 2306 32-33 0.5684845055564764 2306 34-35 0.5929418244199525 2306 36-37 0.6601106080011405 2306 38-39 0.649268612254474 2306 40-41 0.6683056354152015 2306 42-43 0.7083781210330216 2306 44-45 0.706884543669922 2306 46-47 0.6163827074919865 2306 48-49 0.6983477197243246 2306 50-51 0.6796617137195469 2306 52-53 0.7135048692998538 2306 54-55 0.7596443895746816 2306 56-57 0.8235224552450191 2306 58-59 0.7896340618978357 2306 60-61 0.8693511975839598 2306 62-63 0.8676528153411383 2306 64-65 0.677925584336414 2306 66-67 0.7238753260929229 2306 68-69 0.795499806065564 2306 70-71 0.7451740596481429 2306 72-73 0.5256171520613009 2306 74-75 0.7364650784471038 2306 76-77 0.7072662550840576 2306 78-79 0.7406470819396631 2306 80-81 0.8110282802280153 2306 82-83 0.8020312259544227 2306 84-85 0.9072448303360119 2306 86-87 0.8677265672454837 2306 88-89 0.7529824815214354 2306 90-91 0.804789762787756 2306 92-93 0.9843903763334296 2306 94-95 0.7574144661521629 2306 96-97 1.0664699832616549 2306 98-99 1.1360097572798509 2306 100-101 0.8635934592806223 2307 1 0.15356178828842104 2307 2 0.08598339666574617 2307 3 0.08344187600420128 2307 4 0.13146794610980095 2307 5 0.14124317740045456 2307 6 0.22558671580439693 2307 7 0.19144472082252406 2307 8 0.20529228205197825 2307 9 0.22857725271262552 2307 10-11 0.2195868052323462 2307 12-13 0.171987666643183 2307 14-15 0.4351849647643533 2307 16-17 0.4896432809839055 2307 18-19 0.4523371320386431 2307 20-21 0.46205437151783 2307 22-23 0.4744108989698219 2307 24-25 0.4679390844434934 2307 26-27 0.4603268783400054 2307 28-29 0.5316424833332363 2307 30-31 0.5147717557884448 2307 32-33 0.6028644018342035 2307 34-35 0.5567239048899495 2307 36-37 0.4998231417715928 2307 38-39 0.6001488039141947 2307 40-41 0.5192472385105233 2307 42-43 0.5375422483485082 2307 44-45 0.5227096635156201 2307 46-47 0.6314790627349609 2307 48-49 0.5800779343841427 2307 50-51 0.641463100263671 2307 52-53 0.5963367537436142 2307 54-55 0.4554085182911862 2307 56-57 0.48513680543964455 2307 58-59 0.6632246928010943 2307 60-61 0.570966458442463 2307 62-63 0.6162184029878688 2307 64-65 0.5908711383119183 2307 66-67 0.6731101349352357 2307 68-69 0.5914081232287742 2307 70-71 0.43449372368322514 2307 72-73 0.384002381560272 2307 74-75 0.550742536714182 2307 76-77 0.4986295422537381 2307 78-79 0.6407346457736978 2307 80-81 0.4773589352755394 2307 82-83 0.5672486527919247 2307 84-85 0.5434851662075317 2307 86-87 0.48308432972099524 2307 88-89 0.5196922295944404 2307 90-91 0.42205092951163437 2307 92-93 0.6305724764177256 2307 94-95 0.5641316629886504 2307 96-97 0.6931286174059466 2307 98-99 0.7251468215330306 2307 100-101 0.5457661631817636 2201 1 0.13569752280903913 2201 2 0.11130168042911492 2201 3 0.061172993607804926 2201 4 0.15411484246875773 2201 5 0.1657231309029612 2201 6 0.15882377093365108 2201 7 0.17772729513310281 2201 8 0.28913018860000506 2201 9 0.20545273659934082 2201 10-11 0.23586636253008209 2201 12-13 0.21889101353089302 2201 14-15 0.44624398064138404 2201 16-17 0.37891447272616574 2201 18-19 0.35111324528310917 2201 20-21 0.44603469881935354 2201 22-23 0.41040707889914785 2201 24-25 0.5401129087513041 2201 26-27 0.421096061328484 2201 28-29 0.5090418404432882 2201 30-31 0.30419734759992423 2201 32-33 0.3509837601865584 2201 34-35 0.49629369216835784 2201 36-37 0.5670884414652306 2201 38-39 0.5861729355801799 2201 40-41 0.4758916547074463 2201 42-43 0.35258715969047216 2201 44-45 0.45081191806720966 2201 46-47 0.5801766449097414 2201 48-49 0.5220848468929944 2201 50-51 0.5117159081930893 2201 52-53 0.6220659945877571 2201 54-55 0.6774872845103985 2201 56-57 0.5674471273948427 2201 58-59 0.49030903827568295 2201 60-61 0.5576136254452067 2201 62-63 0.5944141627537789 2201 64-65 0.42530823248487337 2201 66-67 0.5656988206933846 2201 68-69 0.5863242347428965 2201 70-71 0.32240992171461613 2201 72-73 0.42835335776359074 2201 74-75 0.30700904986813526 2201 76-77 0.1776518748039777 2201 78-79 0.32428704529719 2201 80-81 0.23249939275230247 2201 82-83 0.2171808809224629 2201 84-85 0.28643522798974175 2201 86-87 0.34106011375611445 2201 88-89 0.2096042038078494 2201 90-91 0.44242918749728943 2201 92-93 0.46907876666767123 2201 94-95 0.3458642047997955 2201 96-97 0.2983489369874732 2201 98-99 0.35419011788577137 2201 100-101 0.35244263514018215 1101 1 0.04944757629026242 1101 2 0.011145162179239776 1101 3 0.08337985108439483 1101 4 0.09011412301650523 1101 5 0.09491939224357537 1101 6 0.10608817005186921 1101 7 0.11924168397045776 1101 8 0.0873861399696878 1101 9 0.09937589893260679 1101 10-11 0.1578439049858602 1101 12-13 0.10965359137981778 1101 14-15 0.24566416111091627 1101 16-17 0.23384701992360846 1101 18-19 0.30555742064834845 1101 20-21 0.20923272223645029 1101 22-23 0.28668278779015566 1101 24-25 0.27854943765172635 1101 26-27 0.1761986891984506 1101 28-29 0.13837574064296376 1101 30-31 0.16534887828443345 1101 32-33 0.2422029534446395 1101 34-35 0.17037154088981055 1101 36-37 0.24586910697517794 1101 38-39 0.285785835375691 1101 40-41 0.3269943232854544 1101 42-43 0.2506619187549646 1101 44-45 0.29760690782166677 1101 46-47 0.3760743339809238 1101 48-49 0.2968093718654998 1101 50-51 0.21948194248670205 1101 52-53 0.28160422005794317 1101 54-55 0.255148250528741 1101 56-57 0.33312751031624543 1101 58-59 0.2077940126483142 1101 60-61 0.2922712646199628 1101 62-63 0.19619910634615678 1101 64-65 0.15731382610152167 1101 66-67 0.29614442416303177 1101 68-69 0.30087532270673023 1101 70-71 0.31922202546029865 1101 72-73 0.06076995717199196 1101 74-75 0.224383884076353 1101 76-77 -0.04892688050208349 1101 78-79 0.2788379754135377 1101 80-81 0.31811576851573165 1101 82-83 0.10321395329511773 1101 84-85 0.31198533688374397 1101 86-87 0.3855738334031855 1101 88-89 0.30057347879355945 1101 90-91 0.23872647995587926 1101 92-93 0.38501054248933997 1101 94-95 0.32700651802155534 1101 96-97 0.419771873972671 1101 98-99 0.5120933756503092 1101 100-101 0.32997532930194495 2304 1 0.047505312795529164 2304 2 0.0421304858998397 2304 3 0.05785619866777836 2304 4 0.09912272172823577 2304 5 0.11057629959497461 2304 6 0.1056811900739234 2304 7 0.0159241963702641 2304 8 0.10262230754388213 2304 9 0.04183842625113954 2304 10-11 0.09095296651134532 2304 12-13 0.1457219981249196 2304 14-15 0.19998957338647472 2304 16-17 0.2128170078634284 2304 18-19 0.2791688388786113 2304 20-21 0.1631557088338198 2304 22-23 0.1614261723790662 2304 24-25 0.23557264268107048 2304 26-27 0.25516598331842744 2304 28-29 0.24573134172429434 2304 30-31 0.25792790846665525 2304 32-33 0.17667345989634242 2304 34-35 0.10911572470988062 2304 36-37 0.21638563627318774 2304 38-39 0.03684441936830751 2304 40-41 0.20976751973914531 2304 42-43 0.20765130568614865 2304 44-45 0.26745763725575245 2304 46-47 0.3315756276927502 2304 48-49 0.33169133507490045 2304 50-51 0.35561364430835596 2304 52-53 0.19293151936400932 2304 54-55 0.23556902518993894 2304 56-57 0.2506673589855808 2304 58-59 0.23181862214618576 2304 60-61 0.1783121226965534 2304 62-63 0.22784024923119972 2304 64-65 0.1774071059124651 2304 66-67 0.10056728876331533 2304 68-69 0.1999480672769849 2304 70-71 0.14381084486758056 2304 72-73 0.0879312194998505 2304 74-75 0.12354257894194376 2304 76-77 -0.08322318014322505 2304 78-79 0.3460603118700831 2304 80-81 0.1630859514183456 2304 82-83 0.23985825608900058 2304 84-85 0.2037986245474599 2304 86-87 0.17392069817478273 2304 88-89 0.10156482387981924 2304 90-91 0.18309105727131936 2304 92-93 0.2877329284617858 2304 94-95 0.2171706646746614 2304 96-97 0.14814394049491142 2304 98-99 0.21756341911383004 2304 100-101 0.21816504166206485 2202 1 0.06666112340623442 2202 2 0.1123611078466098 2202 3 0.08700728078791542 2202 4 0.14694965221586642 2202 5 0.10721354078835788 2202 6 0.08448585113148965 2202 7 0.11160846621681486 2202 8 0.11778690934149694 2202 9 0.157962792488469 2202 10-11 0.16570793058176037 2202 12-13 0.10612431282685719 2202 14-15 0.18328443327360588 2202 16-17 0.09798973114146037 2202 18-19 0.12026479238256371 2202 20-21 0.15655182670571577 2202 22-23 0.08795131509181431 2202 24-25 0.07413407900408941 2202 26-27 0.04887388992727182 2202 28-29 0.1043766816358982 2202 30-31 0.11201885304265602 2202 32-33 0.14818488216192804 2202 34-35 0.28912379461969806 2202 36-37 0.15884974002327112 2202 38-39 0.28645217160323 2202 40-41 0.25526470389544187 2202 42-43 0.16255077057892464 2202 44-45 0.15545073888771554 2202 46-47 0.14773710697942022 2202 48-49 0.24713351118352023 2202 50-51 0.03667019787359038 2202 52-53 0.19342526340560084 2202 54-55 0.11973638850428614 2202 56-57 0.13589254201231427 2202 58-59 0.20519823386934632 2202 60-61 0.0570074896471624 2202 62-63 0.23514753656604626 2202 64-65 0.12112935448310935 2202 66-67 0.07261540093257679 2202 68-69 0.10385710100576517 2202 70-71 0.13987147339939554 2202 72-73 0.1422218134313411 2202 74-75 0.07744957652115403 2202 76-77 0.060957726677767 2202 78-79 0.2438648980613678 2202 80-81 0.25014975083076507 2202 82-83 0.18253442170460232 2202 84-85 0.3265038439723611 2202 86-87 0.035301147119973564 2202 88-89 0.03224673481597762 2202 90-91 0.15178121203469175 2202 92-93 0.3031836581768843 2202 94-95 0.09966625138716267 2202 96-97 0.1436999773760661 2202 98-99 0.31823180966118514 2202 100-101 0.319089062340808 1102 1 0.07111755863594027 1102 2 0.07976671273211622 1102 3 0.0057012045027846625 1102 4 0.021856713133189487 1102 5 0.050460453619706414 1102 6 -0.03243971904129239 1102 7 0.0017252172620558781 1102 8 0.09939682142932327 1102 9 0.12319189084926307 1102 10-11 0.07430965607476026 1102 12-13 0.02450680981618092 1102 14-15 0.05172719357182132 1102 16-17 0.0055366001178143165 1102 18-19 0.029644007375061676 1102 20-21 -0.05523699249473424 1102 22-23 0.013513615187839889 1102 24-25 -0.06062104412299618 1102 26-27 -0.011552673529358515 1102 28-29 0.003401745272036294 1102 30-31 0.18353495922409024 1102 32-33 0.10169188851742206 1102 34-35 0.009951861543129326 1102 36-37 0.06942046471773722 1102 38-39 -0.08248979960363556 1102 40-41 -0.028193200128107776 1102 42-43 -0.07274828029053282 1102 44-45 -0.09983953767048348 1102 46-47 -0.01479590441923051 1102 48-49 0.027261104983622886 1102 50-51 -0.05963648667981403 1102 52-53 -0.013873806689773005 1102 54-55 -0.01207509739068513 1102 56-57 -0.13506138837858828 1102 58-59 -0.090922174741074 1102 60-61 -0.1129120863484232 1102 62-63 -0.12911623838994046 1102 64-65 -0.06971514728549977 1102 66-67 -0.07335332181344256 1102 68-69 -0.025075552260439338 1102 70-71 -0.13256699620426815 1102 72-73 -0.019691493589000686 1102 74-75 -0.2506369871807763 1102 76-77 -0.14836735741076268 1102 78-79 0.09857886114959058 1102 80-81 0.1528956097171701 1102 82-83 0.1170811922310051 1102 84-85 0.08996347832854923 1102 86-87 -0.037587789060246735 1102 88-89 -0.04453777015475424 1102 90-91 -0.05208644602638657 1102 92-93 -0.10829457957544975 1102 94-95 -0.2752028549159249 1102 96-97 0.0651784272764715 1102 98-99 0.1443861685911827 1102 100-101 0.05911545157084319 2305 1 0.0208965909665082 2305 2 0.06632744512639732 2305 3 0.07169543500670983 2305 4 0.0508730001012907 2305 5 0.06980252645300311 2305 6 0.06401146733265506 2305 7 0.03536981196348421 2305 8 0.11585631637365168 2305 9 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-0.21385001984080887 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 5.0 9 7.0 10 23.0 11 16.0 12 28.0 13 129.0 14 333.0 15 756.0 16 1404.0 17 2221.0 18 3120.0 19 4069.0 20 5345.0 21 6652.0 22 8490.0 23 10640.0 24 13185.0 25 16483.0 26 20931.0 27 26682.0 28 35561.0 29 47008.0 30 53462.0 31 63895.0 32 86172.0 33 120636.0 34 182430.0 35 312309.0 36 594267.0 37 861384.0 38 195652.0 39 394.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.42599642287159 26.085570037858503 21.831575184336778 14.656858354933128 2 32.62103408436808 22.67111096316737 31.193455932982484 13.51439901948207 3 37.593003524344084 18.898907090540447 26.313007982603807 17.19508140251166 4 31.75815941312756 18.69991848162582 31.646263379544536 17.89565872570208 5 31.161328037778514 17.28604186949193 33.90547666538629 17.64715342734327 6 27.095185715316926 17.92874937960249 37.15477005740009 17.821294847680488 7 33.76035881510527 18.845011517794326 27.697574400014364 19.697055267086036 8 31.053200278716037 26.70961357136152 23.395091949736862 18.842094200185585 9 29.01994210994622 25.56883018181995 25.688440203778374 19.722787504455454 10-11 24.05736044842912 28.220896297213326 30.6034471473683 17.118296106989256 12-13 23.740700582603285 27.07065032619725 30.067296532992433 19.12135255820703 14-15 22.739574423203297 27.799063391441564 30.760383874115497 18.700978311239638 16-17 22.474472535885813 27.997983310699187 31.3249596344227 18.2025845189923 18-19 21.593610924830823 27.901917537903625 31.69353279308102 18.810938744184533 20-21 22.117419041631244 28.088775470894333 31.246584774818615 18.54722071265581 22-23 21.39536423271368 28.68325373669114 30.932898328863228 18.98848370173195 24-25 21.69250051146562 27.428283543822786 31.429964367583512 19.44925157712808 26-27 21.54760707023143 27.722932622305734 30.956498680287797 19.772961627175036 28-29 21.44654819614398 28.69200568951737 30.477030050989473 19.384416063349178 30-31 20.564770248147784 28.980951038060148 30.7185689883902 19.735709725401872 32-33 20.301893750544657 29.598206822109823 30.96293173963015 19.136967687715362 34-35 21.116292882231257 28.926924560036717 31.05467763827431 18.902104919457724 36-37 20.501131582618623 29.151988133249603 31.786512941482727 18.56036734264905 38-39 21.103520267316057 29.187388660386453 30.441049800481657 19.268041271815832 40-41 21.04019951460323 28.54827169502511 30.87687087017226 19.534657920199393 42-43 20.871462612143745 29.089153600138236 30.829539262045806 19.209844525672207 44-45 20.532380542389188 28.49132789939294 31.392132742439376 19.584158815778498 46-47 21.152266298884967 28.40765796301924 30.69438743308633 19.745688305009466 48-49 20.740214883479638 29.94707300693431 30.077253848152026 19.235458261434026 50-51 21.508340919614344 29.112524806577884 29.61565045409222 19.763483819715553 52-53 20.857049671343734 28.881038923344953 29.476917760005744 20.78499364530557 54-55 21.772386936148898 28.945924382336802 29.740018695133543 19.541669986380754 56-57 21.24941434579269 29.15361861823949 29.501747377064053 20.09521965890377 58-59 21.003726684840295 29.288114353581495 30.04454253423921 19.663616427338997 60-61 21.10489517290029 29.491511984060402 29.96125438688686 19.44233845615245 62-63 20.614222838115705 29.296207673297186 29.767796313802712 20.3217731747844 64-65 21.11239869185454 28.979955509887585 29.721744159036255 20.18590163922162 66-67 21.18311387101601 29.191437228789468 29.449105940445907 20.176342959748617 68-69 21.284809191291316 29.685854472467838 29.269462937225143 19.7598733990157 70-71 20.556014559346696 30.75442203961416 28.998718509632877 19.69084489140627 72-73 20.396547580191886 30.5509787436207 28.946121638973548 20.106352037213863 74-75 20.880868073676275 29.760254690811415 29.08723945911872 20.27163777639359 76-77 20.82965097716406 29.275531143053858 29.309778224430044 20.585039655352038 78-79 20.60864920587067 29.382467288125575 29.516430558662186 20.49245294734157 80-81 20.113424484353406 29.73694496341055 29.49111399113924 20.658516561096803 82-83 19.549824099195636 29.517897897493555 29.64805626432023 21.284221738990585 84-85 19.726882175696662 29.356210783330987 29.94644315845853 20.97046388251382 86-87 20.021202905785664 29.35175332656122 30.537009975054414 20.090033792598696 88-89 20.167836494552617 29.937362912986465 29.890023647188364 20.004776945272553 90-91 20.48835059579723 29.62666391335137 29.392482476607107 20.492503014244292 92-93 19.900783107166177 30.116846016108063 29.574766841985255 20.4076040347405 94-95 19.604702243552037 30.462643409955188 29.642225397419786 20.290428949072993 96-97 19.85393955979105 30.990622585291867 29.205952777544486 19.949485077372604 98-99 20.412653196521795 30.252043306194004 28.80319087244666 20.532112624837534 100-101 20.41899799752131 30.026593725018113 28.28234612842274 21.272062149037836 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 446.0 1 268.5 2 98.0 3 180.0 4 215.0 5 157.0 6 141.5 7 152.0 8 157.5 9 164.0 10 178.0 11 170.5 12 156.5 13 158.5 14 167.0 15 179.5 16 184.5 17 190.5 18 221.0 19 261.0 20 341.5 21 480.0 22 719.0 23 1060.0 24 1583.0 25 2537.5 26 4403.0 27 7282.5 28 10378.5 29 13468.0 30 17002.5 31 20351.0 32 25946.0 33 37242.0 34 54481.5 35 79571.5 36 115366.5 37 155470.0 38 189425.5 39 206531.5 40 206038.5 41 191747.5 42 173129.5 43 163674.0 44 150697.0 45 134984.5 46 126266.0 47 112514.5 48 93831.0 49 77991.5 50 64132.5 51 52449.5 52 42631.5 53 34189.0 54 27934.5 55 22060.0 56 15729.5 57 10382.5 58 7019.5 59 5083.0 60 3764.0 61 2795.0 62 2022.0 63 1425.5 64 1033.0 65 790.5 66 569.0 67 402.0 68 324.0 69 243.5 70 166.5 71 120.5 72 85.0 73 58.0 74 36.0 75 24.0 76 21.0 77 14.5 78 12.5 79 9.5 80 5.0 81 2.5 82 3.0 83 3.5 84 1.5 85 2.5 86 2.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18955084155262636 2 0.0 3 0.0 4 0.02513381324454714 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 5.610226170657844E-5 48-49 0.0037588515343407554 50-51 1.1220452341315688E-4 52-53 0.002805113085328922 54-55 0.009948801075966577 56-57 0.0148296978444389 58-59 0.015016705383460827 60-61 0.00359054474922102 62-63 0.0024872002689916442 64-65 0.01069683123205429 66-67 0.01828933731634457 68-69 0.027078691650375193 70-71 0.023544249162860752 72-73 0.018382841085855535 74-75 0.024086571026024343 76-77 0.017522606406354665 78-79 0.007293294021855197 80-81 0.003889756811656105 82-83 8.602346795008693E-4 84-85 0.0030295221321552357 86-87 0.017597409421963438 88-89 0.016176152125396786 90-91 0.020496026276803324 92-93 0.020776537585336215 94-95 0.020178113460466045 96-97 0.023095431069208125 98-99 0.03048222886057429 100-101 0.018775556917801584 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2673689.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.20212654105465 #Duplication Level Percentage of deduplicated Percentage of total 1 87.28786213419396 41.20172713956356 2 5.8707416898163345 5.542229842651112 3 1.7985046578035193 2.5467973332695375 4 1.078550179378535 2.0363944819161124 5 0.6553429222227057 1.5466789771270346 6 0.5272178222722604 1.49314814169567 7 0.37438124734051814 1.2370113708095511 8 0.2296939296772384 0.8673633547469692 9 0.23107579147068183 0.9816541874616131 >10 1.6034471378886732 15.223402987067432 >50 0.1869880595305468 6.1778879942628 >100 0.13967041285140666 14.064273481066476 >500 0.014031108953344322 4.270582578263315 >1k 0.00225463041927634 1.578123838581256 >5k 1.5885078858088588E-4 0.3916629934394387 >10k+ 7.942539233232488E-5 0.8410612980779122 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT 22434 0.8390654260835871 TruSeq Adapter, Index 2 (97% over 37bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 5371 0.20088349841735517 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 5076 0.18985005361506144 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 4.488180936526275E-4 0.0 0.0 2 5.236211092613988E-4 0.0 0.0 3 6.358256326745557E-4 0.0 0.0 4 7.106286482833269E-4 0.0 0.0 5 8.228331716964838E-4 0.0 0.0 6 8.97636187305255E-4 0.0 0.0 7 0.0010472422185227976 0.0 0.0 8 0.001309052773153497 0.0 0.0 9 0.0014586588043710394 0.0 0.0 10-11 0.0024497987611872586 0.0 0.0 12-13 0.0034222379641012848 0.0 0.0 14-15 0.00458168470603724 0.0 0.0 16-17 0.006676169143082835 0.0 0.0 18-19 0.008658449056715273 0.0 0.0 20-21 0.011070846310098144 0.0 0.0 22-23 0.014100368442253382 0.0 0.0 24-25 0.017036386804897653 0.0 0.0 26-27 0.020252916476074814 0.0 0.0 28-29 0.02378735896358926 0.0 0.0 30-31 0.0335678532544361 0.0 0.0 32-33 0.0374576100660922 0.0 0.0 34-35 0.04172138195579217 0.0 0.0 36-37 0.0463030666618294 0.0 0.0 38-39 0.05114656192249735 0.0 0.0 40-41 0.056943795632177115 0.0 0.0 42-43 0.06279713160356346 0.0 0.0 44-45 0.06887487662177613 0.0 0.0 46-47 0.07506482616340196 0.0 0.0 48-49 0.08146048399795189 0.0 0.0 50-51 0.08858547123468735 0.0 0.0 52-53 0.09578526148703159 0.0 0.0 54-55 0.10526654371544335 0.0 0.0 56-57 0.11542105308433404 0.0 0.0 58-59 0.12473402852762606 0.0 0.0 60-61 0.13636589745479 0.0 0.0 62-63 0.14893280407706355 0.0 0.0 64-65 0.1615558129610437 0.0 0.0 66-67 0.17526346557135103 0.0 0.0 68-69 0.19263646594648817 0.0 0.0 70-71 0.21195434472745334 0.0 0.0 72-73 0.23297399211351805 0.0 0.0 74-75 0.2560133209210196 0.0 0.0 76-77 0.283017209555786 0.0 0.0 78-79 0.31263920373685944 0.0 0.0 80-81 0.343532849183282 0.0 0.0 82-83 0.3822621105147233 0.0 0.0 84-85 0.4255169542904953 0.0 0.0 86-87 0.4724184450771949 0.0 0.0 88-89 0.527997085674512 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAAGA 4210 0.0 53.485516 4 TCGGAAG 4245 0.0 53.26835 3 GATCGGA 4525 0.0 50.48466 1 GAAGAGC 4560 0.0 49.472416 6 ATCGGAA 4615 0.0 49.100582 2 CGACGGC 50 2.7957436E-5 47.59231 1 CGAGGTG 150 0.0 44.41949 1 TCGTATG 2670 0.0 42.87189 44-45 CGAGGCG 100 6.2573235E-10 42.83308 1 TATGCCG 2645 0.0 42.829777 48-49 ATGCCGT 2685 0.0 42.633976 48-49 GCCGTCT 2710 0.0 42.503582 50-51 CGTATGC 2665 0.0 42.418446 46-47 TGCCGTC 2695 0.0 42.21141 50-51 TCTCGTA 2735 0.0 41.41884 42-43 CTCGTAT 2745 0.0 40.83537 44-45 ATTGACG 105 1.0095391E-9 40.79341 1 CGTCTTC 2840 0.0 40.142128 52-53 GTATGCC 2880 0.0 39.911484 46-47 GGAAGAG 5790 0.0 39.45489 5 >>END_MODULE