FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n01_35005-1.3410000003578e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n01_35005-1.3410000003578e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2673689
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT224340.8390654260835871TruSeq Adapter, Index 2 (97% over 37bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC53710.20088349841735517No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG50760.18985005361506144No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA42100.053.4855164
TCGGAAG42450.053.268353
GATCGGA45250.050.484661
GAAGAGC45600.049.4724166
ATCGGAA46150.049.1005822
CGACGGC502.7957436E-547.592311
CGAGGTG1500.044.419491
TCGTATG26700.042.8718944-45
CGAGGCG1006.2573235E-1042.833081
TATGCCG26450.042.82977748-49
ATGCCGT26850.042.63397648-49
GCCGTCT27100.042.50358250-51
CGTATGC26650.042.41844646-47
TGCCGTC26950.042.2114150-51
TCTCGTA27350.041.4188442-43
CTCGTAT27450.040.8353744-45
ATTGACG1051.0095391E-940.793411
CGTCTTC28400.040.14212852-53
GTATGCC28800.039.91148446-47
GGAAGAG57900.039.454895
Produced by FastQC (version 0.11.2)