FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n01_34344-1.34100000035857.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n01_34344-1.34100000035857.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4943093
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT1082732.1903897013469096TruSeq Adapter, Index 13 (97% over 38bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC82960.16783014197790735No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA74230.15016913499300943No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG61590.12459810082472654No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG168600.067.2261053
CGGAAGA169100.066.9149554
GATCGGA171350.066.3633041
ATCGGAA173350.065.4936452
GAAGAGC177250.063.7148746
GGAAGAG202850.055.9783065
TTGTGTT577700.045.0363541
GCCGTCT129450.044.03407350-51
ATGCCGT130450.043.72961448-49
CGTCTTC131650.043.29997652-53
TCGTATG132000.043.2863444-45
TATGCCG129950.043.22167648-49
TGCCGTC130050.043.1369350-51
TCTCGTA130100.042.82327342-43
CGTATGC131250.042.7375646-47
AATCTCG129450.042.61204540-41
CTCGTAT129200.042.5885344-45
GTATGCC134650.042.43443346-47
ATCTCGT130250.041.91702342-43
CCGTCTT135050.041.55912452-53
Produced by FastQC (version 0.11.2)