##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n01_34344-1.34100000035857.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4943093 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.68583172519716 33.0 33.0 33.0 33.0 33.0 2 32.71989137165738 33.0 33.0 33.0 33.0 33.0 3 32.75231297489244 33.0 33.0 33.0 33.0 33.0 4 36.67707243217961 37.0 37.0 37.0 37.0 37.0 5 36.669141163235246 37.0 37.0 37.0 37.0 37.0 6 36.53122447827706 37.0 37.0 37.0 37.0 37.0 7 36.46412418297612 37.0 37.0 37.0 37.0 37.0 8 36.39640504437202 37.0 37.0 37.0 37.0 37.0 9 36.415348244510064 37.0 37.0 37.0 37.0 37.0 10-11 36.36676914636241 37.0 37.0 37.0 37.0 37.0 12-13 36.339202600477066 37.0 37.0 37.0 37.0 37.0 14-15 38.1479690954631 40.0 37.0 40.0 37.0 40.0 16-17 38.1368646108823 40.0 37.0 40.0 37.0 40.0 18-19 38.15024641454247 40.0 37.0 40.0 37.0 40.0 20-21 38.09657546398581 40.0 37.0 40.0 37.0 40.0 22-23 37.964467287991546 40.0 37.0 40.0 33.0 40.0 24-25 37.9569727901134 40.0 37.0 40.0 33.0 40.0 26-27 37.867130155147805 40.0 37.0 40.0 33.0 40.0 28-29 37.72251665101183 40.0 37.0 40.0 33.0 40.0 30-31 37.669592095475444 40.0 37.0 40.0 33.0 40.0 32-33 37.53963348454096 40.0 37.0 40.0 33.0 40.0 34-35 37.428445307421896 40.0 37.0 40.0 33.0 40.0 36-37 37.35769820636593 40.0 37.0 40.0 33.0 40.0 38-39 37.25371139082352 40.0 37.0 40.0 33.0 40.0 40-41 37.10959443813823 40.0 37.0 40.0 33.0 40.0 42-43 36.963572706400626 37.0 37.0 40.0 33.0 40.0 44-45 36.82726169626993 37.0 37.0 40.0 33.0 40.0 46-47 36.80610075513449 37.0 37.0 40.0 33.0 40.0 48-49 36.592560366555915 37.0 37.0 40.0 33.0 40.0 50-51 36.432121750491035 37.0 37.0 40.0 33.0 40.0 52-53 36.28266259607092 37.0 37.0 40.0 33.0 40.0 54-55 36.09350532146573 37.0 37.0 40.0 33.0 40.0 56-57 35.92207642057311 37.0 37.0 40.0 30.0 40.0 58-59 35.68200163339027 37.0 37.0 40.0 27.0 40.0 60-61 35.423639409576154 37.0 33.0 40.0 27.0 40.0 62-63 35.15785278569511 37.0 33.0 40.0 27.0 40.0 64-65 34.88346678891132 37.0 33.0 37.0 27.0 40.0 66-67 34.469883330133584 37.0 33.0 37.0 27.0 40.0 68-69 34.19234495486935 37.0 33.0 37.0 27.0 40.0 70-71 33.814131860355445 37.0 33.0 37.0 27.0 40.0 72-73 33.342215997149964 37.0 33.0 37.0 27.0 37.0 74-75 32.91034580979965 37.0 33.0 37.0 24.5 37.0 76-77 30.333976520368928 33.0 30.0 35.0 22.0 37.0 78-79 32.609094144091564 35.0 33.0 37.0 27.0 37.0 80-81 32.96725875883784 37.0 33.0 37.0 27.0 37.0 82-83 32.87778886620179 37.0 33.0 37.0 27.0 37.0 84-85 32.7010763301439 37.0 33.0 37.0 27.0 37.0 86-87 32.59211054697939 37.0 33.0 37.0 24.5 37.0 88-89 32.4529711457988 33.0 33.0 37.0 22.0 37.0 90-91 32.30335095860021 33.0 33.0 37.0 22.0 37.0 92-93 32.16100637799046 33.0 33.0 37.0 22.0 37.0 94-95 32.023777319180525 33.0 33.0 37.0 22.0 37.0 96-97 31.849628461370237 33.0 33.0 37.0 22.0 37.0 98-99 31.674058023994288 33.0 33.0 37.0 22.0 37.0 100-101 30.004770899515748 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.18086129361910963 2306 2 0.14654197676898661 2306 3 0.15793226314954012 2306 4 0.1795399524838288 2306 5 0.17780308966256086 2306 6 0.18415492073290096 2306 7 0.243654400014087 2306 8 0.2365652577271007 2306 9 0.28453283778397065 2306 10-11 0.2582922282154527 2306 12-13 0.2871102652825428 2306 14-15 0.4220253019007174 2306 16-17 0.4841964974173294 2306 18-19 0.34895208246230425 2306 20-21 0.5283155363893215 2306 22-23 0.45649003469252136 2306 24-25 0.430120031043252 2306 26-27 0.5081649042545493 2306 28-29 0.46444459189998355 2306 30-31 0.5420193189047282 2306 32-33 0.5746353202760304 2306 34-35 0.5972905102649477 2306 36-37 0.5942945015807197 2306 38-39 0.5662555925874528 2306 40-41 0.4997441832571141 2306 42-43 0.5174259674567878 2306 44-45 0.5568254565934936 2306 46-47 0.5357458467096521 2306 48-49 0.5520048660653885 2306 50-51 0.5051435109159357 2306 52-53 0.481211653304463 2306 54-55 0.556004387101602 2306 56-57 0.678912093182312 2306 58-59 0.5615311058563819 2306 60-61 0.5433611866940211 2306 62-63 0.5340138559839716 2306 64-65 0.5608378574324107 2306 66-67 0.5398769016665099 2306 68-69 0.7069325259828503 2306 70-71 0.49196045126168286 2306 72-73 0.2749579478594413 2306 74-75 0.441508262555665 2306 76-77 0.34498166533870744 2306 78-79 0.45296984404612317 2306 80-81 0.4282226231763957 2306 82-83 0.43922983022856954 2306 84-85 0.44877589869110324 2306 86-87 0.4877831911515216 2306 88-89 0.3470834506791647 2306 90-91 0.4689089570709797 2306 92-93 0.4984943927258172 2306 94-95 0.3690398784450437 2306 96-97 0.4178296970441586 2306 98-99 0.5141412946003037 2306 100-101 0.4764214869398984 2307 1 0.1299642836523276 2307 2 0.11013001663609856 2307 3 0.08196603945075509 2307 4 0.17612633122136856 2307 5 0.160154972276068 2307 6 0.19528725738849317 2307 7 0.17474105560655318 2307 8 0.2455921126551175 2307 9 0.22757547344288298 2307 10-11 0.1766705504745829 2307 12-13 0.2328626185494258 2307 14-15 0.39519321441456157 2307 16-17 0.42006582189130626 2307 18-19 0.45663895953871503 2307 20-21 0.48818472243583244 2307 22-23 0.4075296249472231 2307 24-25 0.5024089291606302 2307 26-27 0.5019335919621923 2307 28-29 0.4934728311026433 2307 30-31 0.46626765777516255 2307 32-33 0.5872758241519946 2307 34-35 0.5613422821364935 2307 36-37 0.39661593015214436 2307 38-39 0.44400406988534513 2307 40-41 0.4529667746192487 2307 42-43 0.42733668174250283 2307 44-45 0.41871914430112867 2307 46-47 0.4454966772744342 2307 48-49 0.5075710343931803 2307 50-51 0.490526954991239 2307 52-53 0.40769351930667597 2307 54-55 0.41327805819794605 2307 56-57 0.4098063345998142 2307 58-59 0.5083528112827338 2307 60-61 0.4344319231281304 2307 62-63 0.43336947613901344 2307 64-65 0.42600189397726496 2307 66-67 0.5520875882667298 2307 68-69 0.582439862638445 2307 70-71 0.43123716776224086 2307 72-73 0.2589910320233457 2307 74-75 0.4239155161547856 2307 76-77 0.49981416810725676 2307 78-79 0.45413194260648027 2307 80-81 0.40853131642113283 2307 82-83 0.3246638003282385 2307 84-85 0.4424940603743792 2307 86-87 0.4604949851714508 2307 88-89 0.350099092982596 2307 90-91 0.30187891831128866 2307 92-93 0.4303368622717727 2307 94-95 0.2159245683675195 2307 96-97 0.39377308021137836 2307 98-99 0.46751754044748495 2307 100-101 0.33344391218907177 2201 1 0.08610420537444696 2201 2 0.08928037540974287 2201 3 0.07778306489107223 2201 4 0.14617688568973364 2201 5 0.16482310665310962 2201 6 0.16789815106103134 2201 7 0.1894874678700873 2201 8 0.2276085836270667 2201 9 0.22620561042918297 2201 10-11 0.2641481271216861 2201 12-13 0.2053287796714116 2201 14-15 0.3288017434426038 2201 16-17 0.4083526777295319 2201 18-19 0.38775849639455373 2201 20-21 0.43982570417099254 2201 22-23 0.45531582846972896 2201 24-25 0.442251150295661 2201 26-27 0.4359416643692384 2201 28-29 0.4567596869408632 2201 30-31 0.4359064379447588 2201 32-33 0.4456519657045064 2201 34-35 0.4739482834411177 2201 36-37 0.3546027207587983 2201 38-39 0.4549976282675985 2201 40-41 0.5207994556368547 2201 42-43 0.4417467437124927 2201 44-45 0.4974618057492748 2201 46-47 0.45683881686999683 2201 48-49 0.4297783077134767 2201 50-51 0.5439189967394427 2201 52-53 0.591701591713722 2201 54-55 0.5013549499135408 2201 56-57 0.5615308942867046 2201 58-59 0.45278774278958167 2201 60-61 0.42331605554822005 2201 62-63 0.5522764396093365 2201 64-65 0.4909175006308786 2201 66-67 0.6070737265576653 2201 68-69 0.6689348887310729 2201 70-71 0.4406488605215273 2201 72-73 0.4788275453958164 2201 74-75 0.4318819218951617 2201 76-77 0.3802813892422847 2201 78-79 0.6554345355614686 2201 80-81 0.6156456347512034 2201 82-83 0.5234060540790537 2201 84-85 0.6227615098199095 2201 86-87 0.6579061071544885 2201 88-89 0.4548956520171785 2201 90-91 0.6685027767587783 2201 92-93 0.6411163795581416 2201 94-95 0.5597459153994819 2201 96-97 0.563258973883908 2201 98-99 0.5324474165074911 2201 100-101 0.5533232337807199 1101 1 0.09121916740924263 1101 2 0.07160926308691273 1101 3 0.07908365579055499 1101 4 0.06549666505758722 1101 5 0.13932526549490376 1101 6 0.09555562224865355 1101 7 0.14818478118777278 1101 8 0.18692489475252216 1101 9 0.1988285197466979 1101 10-11 0.18487514120609205 1101 12-13 0.10353505385173634 1101 14-15 0.2823357572562273 1101 16-17 0.2641782353375959 1101 18-19 0.295672024023645 1101 20-21 0.2756094110358873 1101 22-23 0.2946367484627572 1101 24-25 0.24944156329470957 1101 26-27 0.27740879118079675 1101 28-29 0.1793433003301388 1101 30-31 0.27693528185159977 1101 32-33 0.2674604640111866 1101 34-35 0.26945632994895163 1101 36-37 0.3438314634003774 1101 38-39 0.3565293348654208 1101 40-41 0.29986244596658196 1101 42-43 0.29807584560463596 1101 44-45 0.36696292597302005 1101 46-47 0.2566171368311245 1101 48-49 0.41737215427249197 1101 50-51 0.3604949929781682 1101 52-53 0.26508044341019144 1101 54-55 0.31195448754304067 1101 56-57 0.2769415518061109 1101 58-59 0.3492951345971136 1101 60-61 0.27245910292775477 1101 62-63 0.3485984566426765 1101 64-65 0.2846479569922309 1101 66-67 0.27190959708650553 1101 68-69 0.2887746030740175 1101 70-71 0.21055288572286202 1101 72-73 0.09154356867569646 1101 74-75 0.2511787264754659 1101 76-77 0.09158938956871054 1101 78-79 0.32410517865150723 1101 80-81 0.2546245526595712 1101 82-83 0.14313854501896373 1101 84-85 0.2936705138964939 1101 86-87 0.3379989985558467 1101 88-89 0.2682747680048436 1101 90-91 0.3539800217502318 1101 92-93 0.5023759033146789 1101 94-95 0.3068355824482154 1101 96-97 0.37889708411838185 1101 98-99 0.5764826191678374 1101 100-101 0.30425717860042667 2304 1 0.08311743680548034 2304 2 0.0774401839462655 2304 3 0.08680838179309802 2304 4 0.1053895229845665 2304 5 0.12726404563514393 2304 6 0.08771003731127536 2304 7 0.07001936963487054 2304 8 0.06661045242345409 2304 9 0.06166492003232804 2304 10-11 0.09593132911036406 2304 12-13 0.09461857093037906 2304 14-15 0.1316676352639803 2304 16-17 0.1831932350187131 2304 18-19 0.1980562697060222 2304 20-21 0.2378267752028833 2304 22-23 0.2520196506872523 2304 24-25 0.26656192143862967 2304 26-27 0.23501354691715193 2304 28-29 0.2263074514372576 2304 30-31 0.09504018029767991 2304 32-33 0.1581694856706619 2304 34-35 0.21297516501937253 2304 36-37 0.08703581432202867 2304 38-39 0.09535139935767489 2304 40-41 0.21590113755360818 2304 42-43 0.22934359937442395 2304 44-45 0.26550164108363106 2304 46-47 0.2744863812641398 2304 48-49 0.1961891167612677 2304 50-51 0.33297386618814784 2304 52-53 0.25287128635944356 2304 54-55 0.23725247904737046 2304 56-57 0.20058818788167088 2304 58-59 0.29445319738312037 2304 60-61 0.24105595787716538 2304 62-63 0.20303244267698517 2304 64-65 0.15554259539296567 2304 66-67 0.05376873369787205 2304 68-69 0.22703847911206765 2304 70-71 0.12956004421011613 2304 72-73 0.05547993163724385 2304 74-75 0.2541520026412698 2304 76-77 0.03365425819734469 2304 78-79 0.3937192978188335 2304 80-81 0.3309492700890857 2304 82-83 0.30818616197559834 2304 84-85 0.41223344261376127 2304 86-87 0.192436748363221 2304 88-89 0.1975291122876186 2304 90-91 0.18157245112982423 2304 92-93 0.3482181364030481 2304 94-95 0.23001321058116275 2304 96-97 0.40618862075191586 2304 98-99 0.46483896901890986 2304 100-101 0.4645008619960187 2202 1 0.015521303934946218 2202 2 0.05949979854595 2202 3 0.058252204718222345 2202 4 0.11852237414558431 2202 5 0.086856705664907 2202 6 0.10765311922618537 2202 7 0.11880934465116866 2202 8 0.047210298783383564 2202 9 0.12417374121040581 2202 10-11 0.13671089310507512 2202 12-13 0.103007987891921 2202 14-15 0.07869674999586351 2202 16-17 0.008125212719029662 2202 18-19 0.0540792044564995 2202 20-21 0.06353632920286856 2202 22-23 0.1035351118918797 2202 24-25 0.1838667132213203 2202 26-27 0.13934072152115817 2202 28-29 0.13289412900499542 2202 30-31 0.08762785921367566 2202 32-33 0.20571671374241163 2202 34-35 0.19283772028260415 2202 36-37 0.1472020618850749 2202 38-39 0.19270092052943966 2202 40-41 0.19878420795489404 2202 42-43 0.18294482774495435 2202 44-45 0.21111605449794268 2202 46-47 0.2779852870942747 2202 48-49 0.24786437489596835 2202 50-51 0.10253853597136953 2202 52-53 0.23301549425983836 2202 54-55 0.26539586561717243 2202 56-57 0.32648022853815917 2202 58-59 0.3084013785483961 2202 60-61 0.15765061747814002 2202 62-63 0.2774123974600471 2202 64-65 0.19585195699907132 2202 66-67 0.1967589208599847 2202 68-69 0.20796188847160124 2202 70-71 0.11201154101323851 2202 72-73 0.12393999303076697 2202 74-75 0.17107910111280944 2202 76-77 0.10004674170978944 2202 78-79 0.21250495366131616 2202 80-81 0.22752762183985453 2202 82-83 0.05192282863601605 2202 84-85 0.19819753409023377 2202 86-87 0.15932778079577758 2202 88-89 0.2091764739349813 2202 90-91 0.15755268794055866 2202 92-93 0.33951968911791397 2202 94-95 -0.0325501159452557 2202 96-97 0.07652252226599998 2202 98-99 0.09952409124652561 2202 100-101 0.2829702368034219 1102 1 0.04449581577180339 1102 2 0.05396825544921313 1102 3 0.044935921327677875 1102 4 0.09699990906731415 1102 5 0.07630702583390558 1102 6 0.06567626954885952 1102 7 -0.02170913365142013 1102 8 0.08115480164117628 1102 9 0.0712502869041316 1102 10-11 0.05956642867511164 1102 12-13 0.08450849674995453 1102 14-15 0.09764303113887962 1102 16-17 0.13005733791297303 1102 18-19 0.13975217170343512 1102 20-21 0.001467518994381578 1102 22-23 0.022759861628465217 1102 24-25 0.023303920894193197 1102 26-27 0.029172124995319848 1102 28-29 0.1130330238412256 1102 30-31 -0.0030147662497270744 1102 32-33 0.08111100383830916 1102 34-35 0.10268903212016767 1102 36-37 0.009211510869583606 1102 38-39 0.07754236471446774 1102 40-41 0.0864023507683882 1102 42-43 0.15232165803087128 1102 44-45 0.13271009472423856 1102 46-47 0.04340450646366634 1102 48-49 -0.08175859535730012 1102 50-51 -0.04412506593590848 1102 52-53 -0.04435833901089126 1102 54-55 0.012328573218944427 1102 56-57 0.04515706008873366 1102 58-59 0.03818378432978875 1102 60-61 -0.1359802704153381 1102 62-63 0.04359817645880071 1102 64-65 -0.06676870958165892 1102 66-67 0.0685009592341217 1102 68-69 0.044805329039718345 1102 70-71 -0.1006708164227561 1102 72-73 -0.09978886584260493 1102 74-75 -0.06600608862235191 1102 76-77 -0.1677249615235823 1102 78-79 0.10420339021247571 1102 80-81 0.25077867333557435 1102 82-83 0.1148250367049215 1102 84-85 0.05048504342817495 1102 86-87 0.07600258268281834 1102 88-89 0.10513353195234032 1102 90-91 0.07327805414195154 1102 92-93 0.013068567333888836 1102 94-95 -0.1758139537887189 1102 96-97 0.01832162107587365 1102 98-99 0.08509174806565767 1102 100-101 0.05724976504643209 2305 1 0.04883861355028074 2305 2 0.07821643826582658 2305 3 0.0431192483401901 2305 4 0.08581088923270386 2305 5 0.09062808458973137 2305 6 -0.0031765748351801903 2305 7 -0.007855355483947335 2305 8 0.08589925753903316 2305 9 0.06234794276259947 2305 10-11 0.06496932098300334 2305 12-13 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-0.18901381479816592 1213 94-95 -0.04837653574434597 1213 96-97 -0.15846800399054572 1213 98-99 -0.012991110818646234 1213 100-101 0.154126149156955 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 9.0 9 27.0 10 36.0 11 49.0 12 59.0 13 215.0 14 523.0 15 1271.0 16 2400.0 17 3938.0 18 6019.0 19 8407.0 20 11172.0 21 14393.0 22 18469.0 23 22903.0 24 28825.0 25 36285.0 26 45809.0 27 59655.0 28 82653.0 29 115948.0 30 123540.0 31 126272.0 32 162027.0 33 226724.0 34 344308.0 35 580583.0 36 1071098.0 37 1496442.0 38 352851.0 39 183.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.142212438977175 24.906087941778882 23.88517118820822 15.066528431035723 2 28.845785422204273 23.873777005611668 33.64494659517837 13.635490977005693 3 36.944540594320195 19.1247059280495 27.488983112395417 16.441770365234884 4 27.917852732195154 19.216578550269244 34.46133249314667 18.404236224388928 5 33.469550340242435 18.117239550216837 32.17420752553108 16.239002584009647 6 27.00483280407631 19.413108351390516 37.34180198511337 16.240256859419798 7 31.95814847100793 21.58709132116268 29.078413050290575 17.376347157538813 8 27.798910520194543 28.68675543834599 26.418398359084076 17.095935682375387 9 31.750970495598608 23.84104446345638 25.79194848245825 18.616036558486762 10-11 24.92785387610551 28.85153081279272 28.907811364261203 17.31280394684057 12-13 23.963811726787256 27.09411900605552 28.852926699942728 20.089142567214495 14-15 22.92125598284313 27.55793791458101 29.615253040960386 19.905553061615468 16-17 23.1330666851706 27.141842162386993 30.467614103153632 19.257477049288777 18-19 21.156278063147912 27.396905136116196 31.69195886057576 19.75485794016014 20-21 22.477535826252915 27.538466300350812 31.204440215872935 18.779557657523334 22-23 21.336661074351625 29.936893762670458 29.846646623885086 18.879798539092832 24-25 21.386063341312816 27.157985900730573 31.138024309880475 20.317926448076136 26-27 21.258046328482997 27.316469667877985 29.65659355387406 21.768890449764953 28-29 22.00759726754079 28.85739758487247 29.39447022340061 19.740534924186132 30-31 20.451355052393307 28.15903928977262 30.546198503649435 20.843407154184636 32-33 20.34983966516511 29.25683372738486 29.818496233026572 20.574830374423463 34-35 20.599298860045725 29.419990681947517 31.092486020392496 18.888224437614262 36-37 20.480314653193858 28.46776299778297 31.067016137466968 19.984906211556204 38-39 22.204336434697872 29.404403275439083 29.270873519879153 19.120386769983895 40-41 20.85517104371696 30.432959687386013 29.0559675895234 19.65590167937362 42-43 20.89044248206538 29.39739349431621 30.552419305078825 19.15974471853959 44-45 20.496670809147226 28.23220198365679 30.851978710495636 20.419148496700345 46-47 21.88203475497065 28.116078773779524 29.504005742155492 20.497880729094334 48-49 20.82698335339229 29.6173887992322 30.1461946954933 19.40943315188221 50-51 21.998721649688925 28.396451247746977 29.82279604688525 19.782031055678843 52-53 20.601877983282087 28.172419694911678 28.895660132527823 22.330042189278412 54-55 22.061809115225415 28.373430652828795 29.889640525708984 19.675119706236803 56-57 20.847161812375656 28.37891209467795 29.622974439325695 21.1509516536207 58-59 20.751326588132272 28.341599934282648 29.996380284955947 20.910693192629136 60-61 21.75763249612704 28.638180511447924 29.932443880675976 19.67174311174906 62-63 20.553083250775188 28.633409677277736 29.830753951916872 20.9827531200302 64-65 21.956684455246698 28.449302041105774 29.7054854129631 19.888528090684428 66-67 21.069564605098996 30.464764258048906 28.42702863041876 20.038642506433334 68-69 21.20577985146168 30.47523459209634 28.333848494153724 19.985137062288256 70-71 20.923566415001492 31.159955775133064 28.228085461479687 19.68839234838576 72-73 20.643317149387723 31.53376599109291 28.066605783385622 19.75631107613374 74-75 21.104657139087706 30.89296366413301 27.972114146407005 20.03026505037228 76-77 21.16973331881919 30.18964224392419 28.37710777114974 20.263516666106874 78-79 20.942771433210243 29.98051192980451 28.853977048283173 20.22273958870208 80-81 20.58000249649651 30.17085827891045 28.72803907482348 20.52110014976956 82-83 19.84056627305734 30.146830190305312 29.023173311576215 20.98943022506113 84-85 19.97162227256092 29.91056372890662 29.084004506610512 21.033809491921946 86-87 19.995427230236935 29.99040932359428 29.486939279282925 20.527224166885862 88-89 20.135287923877478 30.23306938321624 29.251198013575735 20.38044467933055 90-91 20.321783132320466 29.914463851424255 28.966643158145107 20.797109858110172 92-93 19.951820690657108 30.613862796512375 28.78225586651027 20.652060646320244 94-95 19.67097980267448 30.93425807875822 28.77864444277627 20.61611767579103 96-97 20.076340233658417 31.089129165295788 28.46120039189929 20.373330209146502 98-99 20.36215563191316 30.45870213652902 28.195737869460867 20.983404362096948 100-101 20.38088643850319 30.472736109316777 27.76171956132219 21.384657890857834 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 80.0 1 57.0 2 81.5 3 226.0 4 263.5 5 176.5 6 137.0 7 122.5 8 129.0 9 132.5 10 128.5 11 127.0 12 132.5 13 145.5 14 127.5 15 142.0 16 191.5 17 229.5 18 311.5 19 441.5 20 628.0 21 902.5 22 1382.0 23 2210.5 24 3378.0 25 5089.0 26 7964.0 27 12093.0 28 16581.0 29 21098.0 30 26581.5 31 33480.0 32 46969.0 33 70862.5 34 105496.0 35 147558.5 36 204683.0 37 275062.5 38 336670.0 39 359859.5 40 354628.0 41 331882.5 42 304266.5 43 301829.5 44 292740.0 45 268999.0 46 252016.0 47 222173.5 48 178279.0 49 146742.0 50 123274.0 51 103692.0 52 88204.5 53 71981.5 54 55427.0 55 41126.5 56 30772.5 57 22938.5 58 17690.0 59 13851.0 60 10581.5 61 7893.0 62 5782.5 63 4162.5 64 3034.0 65 2143.0 66 1504.0 67 1068.5 68 727.5 69 535.5 70 381.5 71 251.5 72 170.5 73 117.5 74 88.5 75 68.5 76 49.0 77 39.5 78 30.0 79 18.5 80 14.0 81 8.5 82 6.0 83 4.5 84 2.0 85 1.0 86 1.5 87 1.0 88 0.0 89 1.0 90 1.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19194459825052856 2 0.0 3 0.0 4 0.02698715156684287 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 3.0345372826285078E-5 48-49 0.003317760762340502 50-51 7.080586992799852E-5 52-53 0.0026501625601622306 54-55 0.0095587924402798 56-57 0.014080252991396277 58-59 0.014464627713862557 60-61 0.0033481061351667875 62-63 0.0022657878376959525 64-65 0.010165699896805502 66-67 0.017539625493592777 68-69 0.025702530783863465 70-71 0.021787977689272688 72-73 0.017185596143952785 74-75 0.02203074067188297 76-77 0.01592120560952424 78-79 0.006584945903303863 80-81 0.0033582212594422153 82-83 7.282889478308419E-4 84-85 0.002670392808713087 86-87 0.01604258710082938 88-89 0.015071535170388256 90-91 0.018480332051207613 92-93 0.01881413115229675 94-95 0.018288144689974476 96-97 0.020371860290712716 98-99 0.02898994617337768 100-101 0.01684168191858822 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4943093.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.467273081476016 #Duplication Level Percentage of deduplicated Percentage of total 1 91.14579526778127 43.26442354204078 2 3.9423702911046146 3.742671343923217 3 1.410120875513458 2.008037780276621 4 0.7512512081094769 1.4263938499248525 5 0.4420581862238838 1.0491648321695537 6 0.33071359370843945 0.9418843478588883 7 0.2576435682947048 0.8560746329751469 8 0.17137899832805414 0.6507914971254055 9 0.1728099305098781 0.7382534546432952 >10 1.0817281070098406 10.035050965859641 >50 0.12162902086019586 4.054818061143259 >100 0.13593788317516328 13.70980337854156 >500 0.027552525860542538 8.996374692642897 >1k 0.00884047237595374 5.899551412193529 >5k 1.2755347178828366E-4 0.44154271088112085 >10k+ 4.251767266119596E-5 2.185163497800284 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT 108273 2.1903897013469096 TruSeq Adapter, Index 13 (97% over 38bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 8296 0.16783014197790735 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 7423 0.15016913499300943 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 6159 0.12459810082472654 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0014768081442125406 0.0 0.0 2 0.0015779593869668241 0.0 0.0 3 0.0016386501326193944 0.0 0.0 4 0.0017600316239245347 0.0 0.0 5 0.001962334109433102 0.0 0.0 6 0.002184866843492526 0.0 0.0 7 0.0026906230572639436 0.0 0.0 8 0.003095228028281078 0.0 0.0 9 0.0033986817565439293 0.0 0.0 10-11 0.0051081377590913215 0.0 0.0 12-13 0.007232313856931278 0.0 0.0 14-15 0.009670058807309513 0.0 0.0 16-17 0.011895386147903753 0.0 0.0 18-19 0.014960268803358545 0.0 0.0 20-21 0.018712979909542467 0.0 0.0 22-23 0.0232546707092098 0.0 0.0 24-25 0.02807958498858913 0.0 0.0 26-27 0.03324841349333302 0.0 0.0 28-29 0.03956025104120032 0.0 1.011512427542836E-5 30-31 0.05911278626560334 0.0 2.023024855085672E-5 32-33 0.06685085633630602 0.0 4.046049710171344E-5 34-35 0.0732941904997539 0.0 4.046049710171344E-5 36-37 0.08043546823820633 0.0 6.069074565257016E-5 38-39 0.08833538029731587 0.0 6.069074565257016E-5 40-41 0.0965893217060654 0.0 6.069074565257016E-5 42-43 0.10524786808583209 0.0 6.069074565257016E-5 44-45 0.11586874857503188 0.0 6.069074565257016E-5 46-47 0.1261558299631425 0.0 6.069074565257016E-5 48-49 0.13729258179038914 0.0 6.069074565257016E-5 50-51 0.15071535170388256 0.0 6.069074565257016E-5 52-53 0.16433030897860915 0.0 7.080586992799852E-5 54-55 0.17904781479935739 0.0 8.092099420342688E-5 56-57 0.1946655666806188 0.0 1.011512427542836E-4 58-59 0.21158816959341042 0.0 1.011512427542836E-4 60-61 0.2297347025435289 0.0 1.011512427542836E-4 62-63 0.24958057637191935 0.0 1.011512427542836E-4 64-65 0.27098417933872576 0.0 1.011512427542836E-4 66-67 0.29383424507691847 0.0 1.1126636702971196E-4 68-69 0.3186871054216459 0.0 1.2138149130514032E-4 70-71 0.3469791080200191 0.0 1.2138149130514032E-4 72-73 0.378619216753559 0.0 1.2138149130514032E-4 74-75 0.41265660994037534 0.0 1.2138149130514032E-4 76-77 0.45084120408011746 0.0 1.2138149130514032E-4 78-79 0.4928493151959714 0.0 1.2138149130514032E-4 80-81 0.5392979658687385 0.0 1.2138149130514032E-4 82-83 0.5897117452574734 0.0 1.2138149130514032E-4 84-85 0.6451325111625454 0.0 1.3149661558056869E-4 86-87 0.7043363335466275 0.0 1.4161173985599703E-4 88-89 0.7709646571488742 0.0 1.4161173985599703E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 16860 0.0 67.226105 3 CGGAAGA 16910 0.0 66.914955 4 GATCGGA 17135 0.0 66.363304 1 ATCGGAA 17335 0.0 65.493645 2 GAAGAGC 17725 0.0 63.714874 6 GGAAGAG 20285 0.0 55.978306 5 TTGTGTT 57770 0.0 45.036354 1 GCCGTCT 12945 0.0 44.034073 50-51 ATGCCGT 13045 0.0 43.729614 48-49 CGTCTTC 13165 0.0 43.299976 52-53 TCGTATG 13200 0.0 43.28634 44-45 TATGCCG 12995 0.0 43.221676 48-49 TGCCGTC 13005 0.0 43.13693 50-51 TCTCGTA 13010 0.0 42.823273 42-43 CGTATGC 13125 0.0 42.73756 46-47 AATCTCG 12945 0.0 42.612045 40-41 CTCGTAT 12920 0.0 42.58853 44-45 GTATGCC 13465 0.0 42.434433 46-47 ATCTCGT 13025 0.0 41.917023 42-43 CCGTCTT 13505 0.0 41.559124 52-53 >>END_MODULE