##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n01_34311-1.34100000035764.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2694502 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.688368388666994 33.0 33.0 33.0 33.0 33.0 2 32.72040659090251 33.0 33.0 33.0 33.0 33.0 3 32.746539063619174 33.0 33.0 33.0 33.0 33.0 4 36.68408485130091 37.0 37.0 37.0 37.0 37.0 5 36.67508207453548 37.0 37.0 37.0 37.0 37.0 6 36.550953756946555 37.0 37.0 37.0 37.0 37.0 7 36.49740063284421 37.0 37.0 37.0 37.0 37.0 8 36.41650850509667 37.0 37.0 37.0 37.0 37.0 9 36.429222913918785 37.0 37.0 37.0 37.0 37.0 10-11 36.39156215137342 37.0 37.0 37.0 37.0 37.0 12-13 36.374198460420516 37.0 37.0 37.0 37.0 37.0 14-15 38.19728098179181 40.0 37.0 40.0 37.0 40.0 16-17 38.18998074597829 40.0 37.0 40.0 37.0 40.0 18-19 38.20718244039158 40.0 37.0 40.0 37.0 40.0 20-21 38.14642798557952 40.0 37.0 40.0 37.0 40.0 22-23 38.048027798828876 40.0 37.0 40.0 37.0 40.0 24-25 38.02547168270797 40.0 37.0 40.0 35.0 40.0 26-27 37.92852167116595 40.0 37.0 40.0 33.0 40.0 28-29 37.8127466596796 40.0 37.0 40.0 33.0 40.0 30-31 37.774667452464314 40.0 37.0 40.0 33.0 40.0 32-33 37.661978540004796 40.0 37.0 40.0 33.0 40.0 34-35 37.58099734199492 40.0 37.0 40.0 33.0 40.0 36-37 37.49855056704356 40.0 37.0 40.0 33.0 40.0 38-39 37.41844485548721 40.0 37.0 40.0 33.0 40.0 40-41 37.331066185885184 40.0 37.0 40.0 33.0 40.0 42-43 37.203463014686946 40.0 37.0 40.0 33.0 40.0 44-45 37.076742195774955 40.0 37.0 40.0 33.0 40.0 46-47 37.03738371691689 38.5 37.0 40.0 33.0 40.0 48-49 36.83036141743446 37.0 37.0 40.0 33.0 40.0 50-51 36.64535246216184 37.0 37.0 40.0 33.0 40.0 52-53 36.52339745897387 37.0 37.0 40.0 33.0 40.0 54-55 36.37023372036836 37.0 37.0 40.0 33.0 40.0 56-57 36.19949660456737 37.0 37.0 40.0 33.0 40.0 58-59 35.920983357963735 37.0 37.0 40.0 33.0 40.0 60-61 35.666831570360685 37.0 35.0 40.0 27.0 40.0 62-63 35.40127192334613 37.0 33.0 40.0 27.0 40.0 64-65 35.135749945630025 37.0 33.0 37.0 27.0 40.0 66-67 34.814818285531054 37.0 33.0 37.0 27.0 40.0 68-69 34.5521651125143 37.0 33.0 37.0 27.0 40.0 70-71 34.20459235138812 37.0 33.0 37.0 27.0 40.0 72-73 33.890916206408455 37.0 33.0 37.0 27.0 37.0 74-75 33.53933454122506 37.0 33.0 37.0 27.0 37.0 76-77 30.94009690844542 33.0 30.0 35.0 24.5 37.0 78-79 33.27126608182142 35.0 33.0 37.0 27.0 37.0 80-81 33.677764573936116 37.0 33.0 37.0 27.0 37.0 82-83 33.62764696407722 37.0 33.0 37.0 27.0 37.0 84-85 33.46239119510767 37.0 33.0 37.0 27.0 37.0 86-87 33.38069372373819 37.0 33.0 37.0 27.0 37.0 88-89 33.27321115367515 37.0 33.0 37.0 27.0 37.0 90-91 33.1458249056783 37.0 33.0 37.0 27.0 37.0 92-93 33.02107569413569 35.0 33.0 37.0 27.0 37.0 94-95 32.909486799416 33.0 33.0 37.0 27.0 37.0 96-97 32.74835869485345 33.0 33.0 37.0 27.0 37.0 98-99 32.58501478195228 33.0 33.0 37.0 27.0 37.0 100-101 30.93957195058679 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.1291143319156376 2306 2 0.05903248216959156 2306 3 0.12665839274974644 2306 4 0.20777773743085248 2306 5 0.17572991161254237 2306 6 0.21893062173705147 2306 7 0.21498272153360176 2306 8 0.18732222997739 2306 9 0.2337600689418906 2306 10-11 0.2092281577045796 2306 12-13 0.20486162239143368 2306 14-15 0.4282799541335862 2306 16-17 0.5281928188902825 2306 18-19 0.4265221434137203 2306 20-21 0.5176930604889449 2306 22-23 0.4752965112632026 2306 24-25 0.44109112953101004 2306 26-27 0.5709817430234594 2306 28-29 0.5361824619042466 2306 30-31 0.6325473911680959 2306 32-33 0.6371974943168226 2306 34-35 0.6480283784875027 2306 36-37 0.6503886396223706 2306 38-39 0.6969245362570717 2306 40-41 0.6772741244406077 2306 42-43 0.6757019609237105 2306 44-45 0.678214301021903 2306 46-47 0.6906610326408043 2306 48-49 0.5402033514270457 2306 50-51 0.7355964436935594 2306 52-53 0.6029314756344775 2306 54-55 0.6918582646220912 2306 56-57 0.74802546925266 2306 58-59 0.7368362910409658 2306 60-61 0.5851671096846403 2306 62-63 0.6704737484939614 2306 64-65 0.6141261554167983 2306 66-67 0.6544423312065177 2306 68-69 0.807814900125635 2306 70-71 0.677815893510207 2306 72-73 0.47829043314123254 2306 74-75 0.6383785465874894 2306 76-77 0.46821259583739305 2306 78-79 0.6016654156050052 2306 80-81 0.5611133630672995 2306 82-83 0.6856820302799989 2306 84-85 0.5938844034574515 2306 86-87 0.60900262318156 2306 88-89 0.6147421467919472 2306 90-91 0.7055222831724137 2306 92-93 0.7230565842691234 2306 94-95 0.6224837495041058 2306 96-97 0.7219126060302798 2306 98-99 0.8147734898355949 2306 100-101 0.6853732244223671 2307 1 0.014174974740619461 2307 2 0.06240471962197347 2307 3 0.12149221326895088 2307 4 0.14552718494255146 2307 5 0.15496076639663414 2307 6 0.14378627010743372 2307 7 0.1603963965281281 2307 8 0.3042145756266308 2307 9 0.2784274991446978 2307 10-11 0.1810473632138141 2307 12-13 0.2387833709740974 2307 14-15 0.47157214198158925 2307 16-17 0.48654316764631034 2307 18-19 0.48988630402346445 2307 20-21 0.5326175318222397 2307 22-23 0.4825805271802963 2307 24-25 0.590087651168254 2307 26-27 0.5391120331926587 2307 28-29 0.4859397745739926 2307 30-31 0.5165388569961706 2307 32-33 0.5967413989183896 2307 34-35 0.5888877217162403 2307 36-37 0.5352602379174627 2307 38-39 0.44591259617060075 2307 40-41 0.6191791556004134 2307 42-43 0.6193163168747517 2307 44-45 0.663515664321622 2307 46-47 0.6060869530796538 2307 48-49 0.5399857238170327 2307 50-51 0.5637446364929488 2307 52-53 0.5030674292155481 2307 54-55 0.4376662446218589 2307 56-57 0.6022172345521781 2307 58-59 0.6495074817583131 2307 60-61 0.5953109901179445 2307 62-63 0.6775746628978183 2307 64-65 0.4885527604345725 2307 66-67 0.4746408902230783 2307 68-69 0.6387846108337314 2307 70-71 0.5481273839933181 2307 72-73 0.3666427996929116 2307 74-75 0.27048982589540316 2307 76-77 0.3834810263348025 2307 78-79 0.4224531822731237 2307 80-81 0.44021202818085925 2307 82-83 0.4518176742582085 2307 84-85 0.4169054708855029 2307 86-87 0.19213127619117643 2307 88-89 0.14557310221112374 2307 90-91 0.2591229164149951 2307 92-93 0.3514314041037494 2307 94-95 0.1886555609836904 2307 96-97 0.35764983300172304 2307 98-99 0.37861096927205296 2307 100-101 0.23008168974019583 2201 1 0.053547794630375734 2201 2 0.03463456270398524 2201 3 0.07622200063543261 2201 4 0.14756906304004502 2201 5 0.1291049182256785 2201 6 0.15524983637779144 2201 7 0.15137893412843084 2201 8 0.2226916127593057 2201 9 0.13189873033493882 2201 10-11 0.2299483071501598 2201 12-13 0.13744258636111084 2201 14-15 0.3606325322362096 2201 16-17 0.378323628479059 2201 18-19 0.40095509694843656 2201 20-21 0.46636100830221494 2201 22-23 0.43988552474802844 2201 24-25 0.4478804862280086 2201 26-27 0.46069223319988595 2201 28-29 0.4524758747789406 2201 30-31 0.41181325790053336 2201 32-33 0.4168025246179994 2201 34-35 0.4925279054468419 2201 36-37 0.34954769777345973 2201 38-39 0.4762007107801338 2201 40-41 0.48196794455997605 2201 42-43 0.44202335386140845 2201 44-45 0.46909766874351533 2201 46-47 0.5211684168687896 2201 48-49 0.5116276720008202 2201 50-51 0.5975962395247976 2201 52-53 0.5746346033162126 2201 54-55 0.5659437586794596 2201 56-57 0.5183791953699455 2201 58-59 0.3962608033644912 2201 60-61 0.4982795135334541 2201 62-63 0.6461464932797654 2201 64-65 0.46594570400511515 2201 66-67 0.6366409009819449 2201 68-69 0.48993269656340743 2201 70-71 0.485321380856341 2201 72-73 0.6147986156641352 2201 74-75 0.6594164988790538 2201 76-77 0.2862962359827357 2201 78-79 0.639282269314613 2201 80-81 0.5566797711947018 2201 82-83 0.4566437918540913 2201 84-85 0.6221753146437905 2201 86-87 0.6453229306964872 2201 88-89 0.5825515376114581 2201 90-91 0.70481141818982 2201 92-93 0.709654593598799 2201 94-95 0.7612175454709487 2201 96-97 0.633253808554521 2201 98-99 0.6344082661851118 2201 100-101 0.7121216927219365 1101 1 0.12793612276008304 1101 2 0.10383766284705587 1101 3 0.0718468022128178 1101 4 0.11622505237873071 1101 5 0.14204288441738555 1101 6 0.1325742024695984 1101 7 0.19449986139262165 1101 8 0.13674148140813003 1101 9 0.17765598678666805 1101 10-11 0.21165869225720968 1101 12-13 0.12951780073327512 1101 14-15 0.30153305225224614 1101 16-17 0.3261072356707757 1101 18-19 0.26614858321137547 1101 20-21 0.2806708741506725 1101 22-23 0.30312567763001397 1101 24-25 0.32506394656262927 1101 26-27 0.33224974281019826 1101 28-29 0.3603438306543012 1101 30-31 0.41708418498077293 1101 32-33 0.453871927249871 1101 34-35 0.4306686333134806 1101 36-37 0.4412568731382649 1101 38-39 0.5041430517374437 1101 40-41 0.5617035931538723 1101 42-43 0.3601028112043636 1101 44-45 0.41632336617940524 1101 46-47 0.4791464289917471 1101 48-49 0.41699239197794924 1101 50-51 0.4264363217699554 1101 52-53 0.43786934590188764 1101 54-55 0.5141536972206566 1101 56-57 0.5515892172297043 1101 58-59 0.45825987755458186 1101 60-61 0.5647235789686036 1101 62-63 0.6088458545834641 1101 64-65 0.48872326789584264 1101 66-67 0.47569475701914854 1101 68-69 0.5338632205679232 1101 70-71 0.5148063054984888 1101 72-73 0.36960171442216705 1101 74-75 0.3649031236527165 1101 76-77 0.25230074730421137 1101 78-79 0.6350272876760741 1101 80-81 0.6607554772546038 1101 82-83 0.5343339156178644 1101 84-85 0.6556796566230005 1101 86-87 0.6690392892034964 1101 88-89 0.5000708128741351 1101 90-91 0.6244022959108406 1101 92-93 0.7095110043346438 1101 94-95 0.5532625271073144 1101 96-97 0.6557926701719055 1101 98-99 0.5872516906949201 1101 100-101 0.5480873516235327 2304 1 0.067951815960825 2304 2 0.05445008337662216 2304 3 0.10688410269301585 2304 4 0.15439135048686836 2304 5 0.15442146902406506 2304 6 0.20382519082786388 2304 7 0.14681599461367512 2304 8 0.21830293479297325 2304 9 0.11521323924711879 2304 10-11 0.15930664065044908 2304 12-13 0.13119901421223545 2304 14-15 0.30215620008493005 2304 16-17 0.34545424825747517 2304 18-19 0.2303771454795509 2304 20-21 0.20270818989819617 2304 22-23 0.3257873063480119 2304 24-25 0.25239440294308935 2304 26-27 0.26223942531417066 2304 28-29 0.3402834724989745 2304 30-31 0.22336546290390658 2304 32-33 0.21657444025120043 2304 34-35 0.3048427916822263 2304 36-37 0.27463182331857894 2304 38-39 0.20965588110983902 2304 40-41 0.28733031675425735 2304 42-43 0.2724589367756778 2304 44-45 0.37770969533645626 2304 46-47 0.4804476669305586 2304 48-49 0.4021298541310614 2304 50-51 0.4247504879170023 2304 52-53 0.42910961901017686 2304 54-55 0.5047940248091933 2304 56-57 0.4373122013002657 2304 58-59 0.38001002701363973 2304 60-61 0.43661346460832107 2304 62-63 0.42945340564060785 2304 64-65 0.2751372389425697 2304 66-67 0.38257131991088045 2304 68-69 0.506926641026773 2304 70-71 0.5077499899695326 2304 72-73 0.37453858404244755 2304 74-75 0.4704702323753409 2304 76-77 0.2855559896207325 2304 78-79 0.43101999712767025 2304 80-81 0.42611289733418545 2304 82-83 0.5083963412901085 2304 84-85 0.40759058304414 2304 86-87 0.5084439278982842 2304 88-89 0.518725271575228 2304 90-91 0.4876300851703661 2304 92-93 0.5787836489885123 2304 94-95 0.5479580364252072 2304 96-97 0.4660988965075532 2304 98-99 0.7669595303848169 2304 100-101 0.5564939212328817 2202 1 0.017065943440520925 2202 2 0.027219269059415296 2202 3 0.011496135621172243 2202 4 0.05420769354313393 2202 5 0.05707890387810721 2202 6 0.07125235656199891 2202 7 0.11105952971385591 2202 8 0.06672657647703772 2202 9 0.15320219936732116 2202 10-11 0.14913973427237437 2202 12-13 0.07463461223374424 2202 14-15 0.12974486407384944 2202 16-17 0.038182313932559 2202 18-19 0.11008285221895164 2202 20-21 0.11476726341359722 2202 22-23 0.1253776958205819 2202 24-25 0.03596322715861788 2202 26-27 -0.017333508344243853 2202 28-29 0.038842079746444824 2202 30-31 -0.009034475852750745 2202 32-33 0.10397521215117678 2202 34-35 0.1830527686465473 2202 36-37 0.09074402945284987 2202 38-39 0.0982253374911295 2202 40-41 0.03655107196212981 2202 42-43 0.05196993280582518 2202 44-45 0.11166492630716363 2202 46-47 0.08682134120952867 2202 48-49 0.15553532744416998 2202 50-51 1.8503793190660645E-4 2202 52-53 0.16189981963694322 2202 54-55 0.0329191892453764 2202 56-57 0.19818737085223148 2202 58-59 0.07870666929989767 2202 60-61 0.06010905625700502 2202 62-63 0.037632132422217524 2202 64-65 -0.0836868084968998 2202 66-67 -0.0675825923547464 2202 68-69 0.02687512499826994 2202 70-71 0.037019995640754644 2202 72-73 0.14152803893285437 2202 74-75 0.02657804989064516 2202 76-77 0.02481118141909633 2202 78-79 0.13885298770291854 2202 80-81 0.021106674338263076 2202 82-83 0.13842004933330543 2202 84-85 0.1320387534906402 2202 86-87 -0.02933327496187843 2202 88-89 0.024154771572305833 2202 90-91 0.01322338918289745 2202 92-93 0.014915448607311532 2202 94-95 -0.13956525870046477 2202 96-97 0.09791779088252639 2202 98-99 0.12549343931786439 2202 100-101 0.19898106963178108 1102 1 0.027615861296929722 1102 2 0.02112764268652967 1102 3 0.04553013649356075 1102 4 0.12778456142521577 1102 5 0.10052239947577846 1102 6 0.13277748176007975 1102 7 0.09257985630052445 1102 8 0.11329519285565937 1102 9 0.106665707583943 1102 10-11 0.08735736133895244 1102 12-13 0.09985392533962312 1102 14-15 0.12280971610244507 1102 16-17 0.12400015844249168 1102 18-19 0.08721792058159394 1102 20-21 0.03948201959543951 1102 22-23 0.14956500630429304 1102 24-25 0.12804059924625477 1102 26-27 0.1446989344852696 1102 28-29 0.15981552525484943 1102 30-31 0.1305639566677783 1102 32-33 0.22214503380445905 1102 34-35 0.10857314944346541 1102 36-37 0.09811361238023864 1102 38-39 0.12990771760239284 1102 40-41 0.12949254546712297 1102 42-43 0.17000724050372895 1102 44-45 0.19221383525050584 1102 46-47 0.2820776427751497 1102 48-49 0.3235805909988656 1102 50-51 0.1183528517979866 1102 52-53 0.15496316582513003 1102 54-55 0.12261055721604919 1102 56-57 0.08440732494418057 1102 58-59 0.13460622572951308 1102 60-61 -0.10958234183047466 1102 62-63 0.04220060122848679 1102 64-65 -0.005614254094652438 1102 66-67 0.09798380692486575 1102 68-69 0.06482707162325596 1102 70-71 -0.11294865778154417 1102 72-73 -0.04361127389193342 1102 74-75 -0.09051082932739973 1102 76-77 -0.08574636602867969 1102 78-79 0.13822530322868687 1102 80-81 0.23070535281272697 1102 82-83 0.1886287839103602 1102 84-85 0.2923259272942431 1102 86-87 0.28924633605946326 1102 88-89 0.24973393033958047 1102 90-91 0.21063806199138924 1102 92-93 0.12781784053439793 1102 94-95 0.02591631156982288 1102 96-97 0.2694269893086201 1102 98-99 0.14878552768258402 1102 100-101 0.08981893428985899 2305 1 0.11477203485134169 2305 2 0.06558604235047483 2305 3 0.03738381648966538 2305 4 0.05833056805094827 2305 5 0.12196697260436196 2305 6 -0.0024386721467806183 2305 7 0.0030029404386837655 2305 8 0.10659952264364136 2305 9 0.11899528576616802 2305 10-11 0.08780224302594064 2305 12-13 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Count 7 8.0 8 39.0 9 118.0 10 86.0 11 46.0 12 30.0 13 112.0 14 335.0 15 890.0 16 1726.0 17 2739.0 18 3485.0 19 4195.0 20 5026.0 21 6207.0 22 7749.0 23 9832.0 24 11937.0 25 14822.0 26 18885.0 27 24925.0 28 32729.0 29 40307.0 30 46856.0 31 59334.0 32 80792.0 33 116359.0 34 178665.0 35 311947.0 36 612297.0 37 902579.0 38 199352.0 39 93.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.95395355530493 24.316773777797113 25.09594636151243 13.633326305385527 2 32.46488590470521 20.99081759820553 33.77369918448752 12.770597312601733 3 38.50808052842417 17.45049734607731 28.007513076627887 16.033909048870626 4 31.643889265264075 17.158352899687248 34.86332631110039 16.33443152394829 5 30.36022983096691 16.10297561478893 37.44988869928469 16.08690585495947 6 25.317999392837713 16.873322046151756 41.67857362881898 16.130104932191554 7 34.35495687143673 17.84407656776651 30.12270913140907 17.678257429387696 8 30.732691977961046 27.76427703523694 24.44845095680018 17.054580030001834 9 29.15599988420866 26.488271302081053 25.478177414602033 18.87755139910826 10-11 24.60990936358555 27.41278351250064 30.23079960601254 17.746507517901268 12-13 24.354463273732957 25.98446763075329 29.673015644449325 19.988053451064427 14-15 23.149769419358382 26.907977800721618 30.83779488751539 19.104457892404607 16-17 22.263371858695965 27.909535788060282 31.566241182971844 18.26085117027191 18-19 20.945837115726764 28.145738989987763 31.889009546105367 19.019414348180106 20-21 21.55264683418309 28.065631422800948 31.554624936259096 18.82709680675687 22-23 20.83201274298553 28.29454199699982 31.826864481822614 19.046580778192038 24-25 21.552498383745863 27.025698997439974 31.9701748226574 19.451627796156767 26-27 21.326092910675147 27.36689377109388 31.742730196526107 19.564283121704864 28-29 21.057249168863116 28.65113850351568 30.978154775910355 19.313457551710854 30-31 20.129359710996688 28.921169848825496 30.943862724911693 20.005607715266123 32-33 19.604271958231987 29.78565241369277 31.43271001468917 19.177365613386073 34-35 20.200894265433835 29.452455407344292 31.65482898138506 18.691821345836818 36-37 20.462241260166074 30.21612156903205 31.376688530941895 17.94494863985998 38-39 21.013140832703037 30.224972926351512 30.408123653276192 18.353762587669262 40-41 21.164504609757202 29.52510334006061 29.91322700818185 19.39716504200034 42-43 20.41282582087525 29.70717780131542 30.950765670242593 18.92923070756674 44-45 19.8648395881688 29.23382502592316 31.856350449916164 19.04498493599188 46-47 20.521424931740608 28.999469660616196 31.237156712875592 19.2419486947676 48-49 20.51583721994888 30.6177468683618 29.83072461681122 19.03569129487809 50-51 20.746498284190334 29.720610554720622 29.813206566486073 19.71968459460297 52-53 19.946452377205667 29.507493995005984 29.829844894493217 20.71620873329513 54-55 21.14743212867862 29.342729016530228 29.97632706509055 19.533511789700604 56-57 20.982915302176718 29.465577734864716 29.358268275504322 20.19323868745424 58-59 20.548669777562242 29.525446446550667 30.31671856751072 19.609165208376368 60-61 20.40067458282085 29.85233108893018 30.37289109362138 19.374103234627597 62-63 20.36875249588411 29.853130673729122 29.867308037748902 19.910808792637866 64-65 20.970693608417317 29.653283943423066 29.57786287804484 19.798159570114773 66-67 21.100403545888287 29.987358915305474 29.15040440891932 19.761833129886913 68-69 21.910576394434106 30.02389222808391 28.69894270579854 19.36658867168344 70-71 20.96209324334934 31.07014716470052 28.790541101119143 19.177218490831 72-73 20.22433560214098 31.165484626110736 28.944734399542387 19.665445372205898 74-75 21.09897758520046 30.13657872928049 28.766299753050216 19.99814393246884 76-77 21.289966477842377 29.24317353980399 29.193805286411962 20.27305469594167 78-79 21.088673594633406 29.269694469466433 29.66484392774813 19.97678800815203 80-81 19.6900377932989 29.82313641699633 30.048289169397336 20.43853662030743 82-83 19.07293446733053 29.676685357344397 30.175742596764017 21.074637578561063 84-85 19.059277145279072 29.19467508502732 30.65639534624789 21.089652423445717 86-87 19.16531630858282 29.32029367805991 31.592087492439326 19.922302520917945 88-89 19.52375534811538 29.9361204933898 31.008778026741457 19.53134613175336 90-91 19.9338039895782 29.72211261525281 30.068386087590465 20.275697307578522 92-93 19.100178696974623 30.687771766768897 30.173563241430973 20.038486294825507 94-95 18.910257481017897 30.870402025252552 30.402025252553372 19.817315241176175 96-97 19.492342862615487 31.518218123781867 29.509327377214916 19.480111636387733 98-99 20.004736988548625 30.680929110558008 28.908474958834418 20.405858942058945 100-101 19.527707736102517 30.70224507438853 28.651099411996373 21.11894777751258 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1771.0 1 1100.5 2 457.5 3 743.0 4 853.5 5 606.5 6 538.0 7 562.5 8 552.0 9 579.0 10 575.0 11 533.5 12 516.5 13 524.0 14 529.0 15 521.0 16 542.0 17 567.0 18 604.5 19 654.0 20 732.0 21 850.0 22 1044.0 23 1369.0 24 1900.5 25 2602.0 26 3775.0 27 5908.5 28 8162.5 29 10190.0 30 12573.5 31 15911.5 32 23680.5 33 35958.0 34 52740.5 35 79999.0 36 126979.5 37 189546.5 38 239284.5 39 245355.5 40 224676.5 41 198715.0 42 172126.0 43 159016.5 44 141801.0 45 121563.5 46 116324.5 47 104626.0 48 79529.0 49 60468.5 50 48841.5 51 41188.0 52 35342.0 53 29023.0 54 23183.5 55 16938.0 56 11824.5 57 8862.5 58 7062.5 59 5486.5 60 4242.0 61 3424.5 62 2618.5 63 1911.5 64 1370.0 65 931.5 66 636.0 67 464.5 68 351.0 69 259.5 70 171.5 71 139.5 72 111.5 73 68.0 74 52.0 75 37.0 76 23.0 77 19.5 78 20.0 79 14.5 80 8.5 81 6.5 82 4.5 83 1.5 84 2.0 85 1.0 86 0.5 87 1.0 88 1.0 89 1.5 90 1.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1944329601536759 2 0.0 3 0.0 4 0.0251252365001028 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 3.7112609305912555E-5 48-49 0.003915380281773775 50-51 5.566891395886884E-5 52-53 0.00289478352586118 54-55 0.010614206261490991 56-57 0.015754302650359882 58-59 0.015680077431748058 60-61 0.003562810493367606 62-63 0.002449432214190229 64-65 0.010929663440591248 66-67 0.018871761832056536 68-69 0.02742621827706938 70-71 0.022842811027789182 72-73 0.01864908617622106 74-75 0.023547950604601516 76-77 0.017183138108637513 78-79 0.007608084907712075 80-81 0.004045274414344469 82-83 7.979211000771199E-4 84-85 0.0027463330886375293 86-87 0.01720169441329047 88-89 0.016533667445784044 90-91 0.019781020760051393 92-93 0.020634610774087382 94-95 0.019187219011156793 96-97 0.021432531874164502 98-99 0.029968432014524392 100-101 0.017480038983084815 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2694502.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.8264511238461 #Duplication Level Percentage of deduplicated Percentage of total 1 85.62327758048338 33.24448002042145 2 6.37061634848423 4.946968485663957 3 2.171526068652132 2.52937952306039 4 1.160688179771411 1.8026161152768234 5 0.8239199735325555 1.5994944291161166 6 0.5502741717521256 1.281911594054928 7 0.4496530479429526 1.222090246205183 8 0.27056586852752923 0.8404089976132065 9 0.24986112004967398 0.8731098508822298 >10 1.8797055110847967 14.07412137834045 >50 0.21477983330613037 5.892191897938536 >100 0.19541284641781234 17.287689206448356 >500 0.03190454659301129 8.13613467855378 >1k 0.007241389167651171 4.569204018404619 >5k 4.7792852908730036E-4 1.0235637798962596 >10k+ 9.55857054145082E-5 0.6766357781236979 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT 18232 0.6766370928653977 TruSeq Adapter, Index 6 (97% over 36bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6432 0.23870830305562957 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 5517 0.2047502655407196 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 5317 0.19732774367953707 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 5214 0.19350514492102808 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 5100 0.18927430746015406 No Hit TGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT 4358 0.16173675135516694 No Hit GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT 4238 0.1572832382384574 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 3838 0.1424381945160924 No Hit TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA 3692 0.13701975355742915 No Hit GTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGT 3573 0.13260335305002557 No Hit TGTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAG 3148 0.11683049409501273 No Hit GTGTTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAAT 3073 0.11404704839706929 No Hit TGTGTTTGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAA 2702 0.10027827034457575 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 3.7112609305912556E-4 0.0 0.0 2 4.453513116709507E-4 0.0 0.0 3 4.8246392097686327E-4 0.0 0.0 4 5.195765302827758E-4 0.0 0.0 5 5.93801748894601E-4 0.0 0.0 6 6.68026967506426E-4 0.0 0.0 7 7.051395768123386E-4 0.0 0.0 8 8.164774047300763E-4 0.0 0.0 9 8.907026233419014E-4 0.0 3.711260930591256E-5 10-11 0.0017071800280719777 0.0 3.711260930591256E-5 12-13 0.0026349952607197915 0.0 7.422521861182512E-5 14-15 0.004212281156221076 0.0 7.422521861182512E-5 16-17 0.0055112224819280145 0.0 7.422521861182512E-5 18-19 0.007812204258894593 0.0 7.422521861182512E-5 20-21 0.009036920365989708 0.0 7.422521861182512E-5 22-23 0.01037297430100256 0.0 7.422521861182512E-5 24-25 0.012135823243033406 0.0 7.422521861182512E-5 26-27 0.013768778052493559 0.0 7.422521861182512E-5 28-29 0.01605120352480718 0.0 7.422521861182512E-5 30-31 0.023028374074318743 3.711260930591256E-5 7.422521861182512E-5 32-33 0.025255130632673498 3.711260930591256E-5 7.422521861182512E-5 34-35 0.027444774581722335 3.711260930591256E-5 7.422521861182512E-5 36-37 0.03011688245174804 3.711260930591256E-5 7.422521861182512E-5 38-39 0.03262198357989714 3.711260930591256E-5 7.422521861182512E-5 40-41 0.03507141579408737 3.711260930591256E-5 7.422521861182512E-5 42-43 0.03778063627341899 3.711260930591256E-5 7.422521861182512E-5 44-45 0.04093520806442155 3.711260930591256E-5 9.278152326478139E-5 46-47 0.04453513116709507 3.711260930591256E-5 1.1133782791773767E-4 48-49 0.048580405581439536 7.422521861182512E-5 1.1133782791773767E-4 50-51 0.0521617723794601 7.422521861182512E-5 1.1133782791773767E-4 52-53 0.056633841800822565 7.422521861182512E-5 1.1133782791773767E-4 54-55 0.06054922208259634 7.422521861182512E-5 1.1133782791773767E-4 56-57 0.06574498738542409 7.422521861182512E-5 1.1133782791773767E-4 58-59 0.0718128990069408 7.422521861182512E-5 1.1133782791773767E-4 60-61 0.07862306281457576 7.422521861182512E-5 1.1133782791773767E-4 62-63 0.0862868166362467 7.422521861182512E-5 1.1133782791773767E-4 64-65 0.0952866243929305 7.422521861182512E-5 1.1133782791773767E-4 66-67 0.10541836673344462 7.422521861182512E-5 1.1133782791773767E-4 68-69 0.11738718323460143 7.422521861182512E-5 1.1133782791773767E-4 70-71 0.13009825192187646 7.422521861182512E-5 1.1133782791773767E-4 72-73 0.14470206368375307 7.422521861182512E-5 1.1133782791773767E-4 74-75 0.1619223144016965 7.422521861182512E-5 1.1133782791773767E-4 76-77 0.18146210320125944 7.422521861182512E-5 1.1133782791773767E-4 78-79 0.20413790748717203 7.422521861182512E-5 1.1133782791773767E-4 80-81 0.22985694573616944 7.422521861182512E-5 1.1133782791773767E-4 82-83 0.2588047809947812 7.422521861182512E-5 1.1133782791773767E-4 84-85 0.29331950764927994 7.422521861182512E-5 1.1133782791773767E-4 86-87 0.33440316615092514 7.422521861182512E-5 1.1133782791773767E-4 88-89 0.38329902891146495 7.422521861182512E-5 1.1133782791773767E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTACCG 25 0.0049842047 57.108913 1 TCGCGCT 25 0.0050217267 57.0008 2 CGGAAGA 3805 0.0 56.176872 4 TCGGAAG 3855 0.0 55.325035 3 GATCGGA 4000 0.0 53.777557 1 GAAGAGC 4065 0.0 52.459126 6 ATCGGAA 4225 0.0 50.480003 2 CGACGGG 85 7.1067916E-9 44.7913 1 GGAAGAG 4805 0.0 44.77546 5 GCCGTCT 2370 0.0 42.59145 50-51 ATGCCGT 2360 0.0 42.368572 48-49 TCGTATG 2430 0.0 42.218903 44-45 AATCTCG 2395 0.0 41.941128 40-41 TGCCGTC 2375 0.0 41.901752 50-51 TATGCCG 2370 0.0 41.68874 48-49 GTGTCGG 80 2.2304266E-7 41.641914 1 TCTCGTA 2385 0.0 41.62146 42-43 CGTATGC 2370 0.0 41.584667 46-47 CTCGTAT 2365 0.0 41.37095 44-45 CGAGGCG 185 0.0 41.159573 1 >>END_MODULE