FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n01_34311-1.34100000035764.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n01_34311-1.34100000035764.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2694502
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT182320.6766370928653977TruSeq Adapter, Index 6 (97% over 36bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64320.23870830305562957No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC55170.2047502655407196No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG53170.19732774367953707No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG52140.19350514492102808No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC51000.18927430746015406No Hit
TGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT43580.16173675135516694No Hit
GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT42380.1572832382384574No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA38380.1424381945160924No Hit
TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA36920.13701975355742915No Hit
GTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGT35730.13260335305002557No Hit
TGTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAG31480.11683049409501273No Hit
GTGTTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAAT30730.11404704839706929No Hit
TGTGTTTGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAA27020.10027827034457575No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACCG250.004984204757.1089131
TCGCGCT250.005021726757.00082
CGGAAGA38050.056.1768724
TCGGAAG38550.055.3250353
GATCGGA40000.053.7775571
GAAGAGC40650.052.4591266
ATCGGAA42250.050.4800032
CGACGGG857.1067916E-944.79131
GGAAGAG48050.044.775465
GCCGTCT23700.042.5914550-51
ATGCCGT23600.042.36857248-49
TCGTATG24300.042.21890344-45
AATCTCG23950.041.94112840-41
TGCCGTC23750.041.90175250-51
TATGCCG23700.041.6887448-49
GTGTCGG802.2304266E-741.6419141
TCTCGTA23850.041.6214642-43
CGTATGC23700.041.58466746-47
CTCGTAT23650.041.3709544-45
CGAGGCG1850.041.1595731
Produced by FastQC (version 0.11.2)