##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n01_34310-1.34100000035813.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5815882 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.67309120095628 33.0 33.0 33.0 33.0 33.0 2 32.70756714114901 33.0 33.0 33.0 33.0 33.0 3 32.740829335945946 33.0 33.0 33.0 33.0 33.0 4 36.663219439459056 37.0 37.0 37.0 37.0 37.0 5 36.657214675263354 37.0 37.0 37.0 37.0 37.0 6 36.51854422080778 37.0 37.0 37.0 37.0 37.0 7 36.46475874166635 37.0 37.0 37.0 37.0 37.0 8 36.380889089565436 37.0 37.0 37.0 37.0 37.0 9 36.3916171270325 37.0 37.0 37.0 37.0 37.0 10-11 36.34457602475428 37.0 37.0 37.0 37.0 37.0 12-13 36.31597133504428 37.0 37.0 37.0 37.0 37.0 14-15 38.09079293562009 40.0 37.0 40.0 37.0 40.0 16-17 38.03947200097939 40.0 37.0 40.0 35.0 40.0 18-19 38.07356296087163 40.0 37.0 40.0 37.0 40.0 20-21 38.000348098534324 40.0 37.0 40.0 33.0 40.0 22-23 37.901225815792 40.0 37.0 40.0 33.0 40.0 24-25 37.864973704762235 40.0 37.0 40.0 33.0 40.0 26-27 37.742592267174615 40.0 37.0 40.0 33.0 40.0 28-29 37.61850618702374 40.0 37.0 40.0 33.0 40.0 30-31 37.59237833573653 40.0 37.0 40.0 33.0 40.0 32-33 37.48231557999286 40.0 37.0 40.0 33.0 40.0 34-35 37.36340446728458 40.0 37.0 40.0 33.0 40.0 36-37 37.22948453906045 40.0 37.0 40.0 33.0 40.0 38-39 37.13965706319351 40.0 37.0 40.0 33.0 40.0 40-41 37.041720327200586 38.5 37.0 40.0 33.0 40.0 42-43 36.88570873686915 37.0 37.0 40.0 33.0 40.0 44-45 36.740363112594096 37.0 37.0 40.0 33.0 40.0 46-47 36.65707041511503 37.0 37.0 40.0 33.0 40.0 48-49 36.43084316359926 37.0 37.0 40.0 33.0 40.0 50-51 36.233036106991165 37.0 37.0 40.0 33.0 40.0 52-53 36.071109936549604 37.0 37.0 40.0 33.0 40.0 54-55 35.89426530662073 37.0 37.0 40.0 33.0 40.0 56-57 35.67724095846511 37.0 35.0 40.0 27.0 40.0 58-59 35.394353255447754 37.0 33.0 40.0 27.0 40.0 60-61 35.131197125388724 37.0 33.0 38.5 27.0 40.0 62-63 34.85083414691013 37.0 33.0 37.0 27.0 40.0 64-65 34.58036184365501 37.0 33.0 37.0 27.0 40.0 66-67 34.20628298510871 37.0 33.0 37.0 27.0 40.0 68-69 33.900231383649114 37.0 33.0 37.0 27.0 38.5 70-71 33.56100089375954 37.0 33.0 37.0 27.0 37.0 72-73 33.21547514203348 37.0 33.0 37.0 27.0 37.0 74-75 32.83185834925811 35.0 33.0 37.0 27.0 37.0 76-77 30.193420017806414 33.0 30.0 33.0 22.0 37.0 78-79 32.62024134946341 33.0 33.0 37.0 27.0 37.0 80-81 33.10397992944148 37.0 33.0 37.0 27.0 37.0 82-83 32.99720102643073 37.0 33.0 37.0 27.0 37.0 84-85 32.81553417005365 33.0 33.0 37.0 27.0 37.0 86-87 32.69121742841412 33.0 33.0 37.0 27.0 37.0 88-89 32.57113839311045 33.0 33.0 37.0 27.0 37.0 90-91 32.43112833100809 33.0 33.0 37.0 24.5 37.0 92-93 32.252005800667895 33.0 33.0 37.0 22.0 37.0 94-95 32.08143115695951 33.0 33.0 37.0 22.0 37.0 96-97 31.89944405680858 33.0 33.0 37.0 22.0 37.0 98-99 31.706251089688546 33.0 33.0 37.0 22.0 37.0 100-101 29.92014573198012 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.15636473456759603 2306 2 0.13939153903218227 2306 3 0.1058179873333458 2306 4 0.17032423968693422 2306 5 0.14060391266904304 2306 6 0.22271775782551373 2306 7 0.2682821144249701 2306 8 0.22168510854875478 2306 9 0.23174084270409878 2306 10-11 0.3332777500125914 2306 12-13 0.24591812977561034 2306 14-15 0.4522977859157251 2306 16-17 0.4674426554372886 2306 18-19 0.4297711884376625 2306 20-21 0.4518612520811587 2306 22-23 0.4997499904281497 2306 24-25 0.40161203092441156 2306 26-27 0.5022633649731674 2306 28-29 0.5220081858567198 2306 30-31 0.49160866097560074 2306 32-33 0.5734545814699885 2306 34-35 0.6738746938836684 2306 36-37 0.6726079129782434 2306 38-39 0.6689445354351591 2306 40-41 0.5836634905630262 2306 42-43 0.6225381394334306 2306 44-45 0.5524418300105296 2306 46-47 0.6398407862812618 2306 48-49 0.6332525763151295 2306 50-51 0.7051269618705476 2306 52-53 0.5873873626479096 2306 54-55 0.6192236030288001 2306 56-57 0.6843657369985365 2306 58-59 0.5844132799346085 2306 60-61 0.607203877312962 2306 62-63 0.5383321075266778 2306 64-65 0.4520782954574045 2306 66-67 0.5403073716323732 2306 68-69 0.7146086503859763 2306 70-71 0.7201614281808517 2306 72-73 0.49111192549449356 2306 74-75 0.5884699847298478 2306 76-77 0.5247642691316976 2306 78-79 0.7490900947901409 2306 80-81 0.6832402944882006 2306 82-83 0.6799241552983233 2306 84-85 0.7802196827635228 2306 86-87 0.6940030045821786 2306 88-89 0.641976114643029 2306 90-91 0.7417712223069586 2306 92-93 0.9128560812146773 2306 94-95 0.6971821075340472 2306 96-97 0.7689105423541918 2306 98-99 0.8787596027239495 2306 100-101 0.6694003149561034 2307 1 0.08064584072377556 2307 2 0.10148129011951568 2307 3 0.1291791236571882 2307 4 0.10846821901159132 2307 5 0.16721619959609058 2307 6 0.21947603682615835 2307 7 0.170444257759776 2307 8 0.2651125597403876 2307 9 0.1934045525023791 2307 10-11 0.2364828370009846 2307 12-13 0.14911643923623075 2307 14-15 0.43726517038937374 2307 16-17 0.4883860708009067 2307 18-19 0.4671249433854783 2307 20-21 0.47885661666470014 2307 22-23 0.49966665630410034 2307 24-25 0.5290501507698409 2307 26-27 0.47416844964541127 2307 28-29 0.4863309260713109 2307 30-31 0.5313913345382133 2307 32-33 0.5503069667383826 2307 34-35 0.516212395225601 2307 36-37 0.4764361435502096 2307 38-39 0.5878601380643431 2307 40-41 0.6245643383374571 2307 42-43 0.5293588901489485 2307 44-45 0.47784750482864524 2307 46-47 0.5401801577239098 2307 48-49 0.5026849182886721 2307 50-51 0.5576858528406987 2307 52-53 0.5607335559964 2307 54-55 0.4645823431724381 2307 56-57 0.5912348144337969 2307 58-59 0.7082940522592338 2307 60-61 0.624921189458604 2307 62-63 0.5663541496971618 2307 64-65 0.5224865542816488 2307 66-67 0.5491596479888443 2307 68-69 0.5661978834049819 2307 70-71 0.6436589931919272 2307 72-73 0.4932276044563224 2307 74-75 0.4870772852823819 2307 76-77 0.5272376542221622 2307 78-79 0.5410546755478904 2307 80-81 0.5817587799594435 2307 82-83 0.5444851049867339 2307 84-85 0.5168621037577381 2307 86-87 0.41489171056294083 2307 88-89 0.41245227840201437 2307 90-91 0.45821652214743835 2307 92-93 0.6896209361853138 2307 94-95 0.4721589033416933 2307 96-97 0.5533012063627254 2307 98-99 0.4851965508821685 2307 100-101 0.5201520518154616 2201 1 0.11811598206762852 2201 2 0.0880042475827878 2201 3 0.07308404642338218 2201 4 0.11581600237305878 2201 5 0.16630227895024063 2201 6 0.15857540827122563 2201 7 0.20415584337221304 2201 8 0.29445863804819794 2201 9 0.23536634453501648 2201 10-11 0.26395420347050447 2201 12-13 0.23349940416908765 2201 14-15 0.4474334384022072 2201 16-17 0.4536849225220294 2201 18-19 0.4484592708291686 2201 20-21 0.46788519543035534 2201 22-23 0.4945474794125104 2201 24-25 0.49907192521941823 2201 26-27 0.46689300756181495 2201 28-29 0.4738074130930343 2201 30-31 0.47028344684686374 2201 32-33 0.500119448794031 2201 34-35 0.46765203341578854 2201 36-37 0.5315329661617554 2201 38-39 0.6346464881949174 2201 40-41 0.5781089976889859 2201 42-43 0.5244040352322727 2201 44-45 0.49876255102775957 2201 46-47 0.5843548501923266 2201 48-49 0.5701799906923881 2201 50-51 0.6061771741153521 2201 52-53 0.5463129136355533 2201 54-55 0.6533409816974824 2201 56-57 0.6439505637681293 2201 58-59 0.6072612883718662 2201 60-61 0.5514143093079653 2201 62-63 0.5964305998495902 2201 64-65 0.5090605907384642 2201 66-67 0.6157128259521798 2201 68-69 0.6627744795877817 2201 70-71 0.4446513834946373 2201 72-73 0.4658238757062989 2201 74-75 0.48423529079099126 2201 76-77 0.30333168952151723 2201 78-79 0.5173332003601487 2201 80-81 0.5768750560483085 2201 82-83 0.5146397343306504 2201 84-85 0.6789346500117333 2201 86-87 0.65293670925832 2201 88-89 0.5496829123531057 2201 90-91 0.6013374415011086 2201 92-93 0.7344293162429452 2201 94-95 0.6355109550035962 2201 96-97 0.6344859441464976 2201 98-99 0.7359808211047287 2201 100-101 0.7091283468164811 1101 1 0.07631731645936668 1101 2 0.06836577931981225 1101 3 0.06574634885201647 1101 4 0.12339877384842879 1101 5 0.12750112140560077 1101 6 0.16281576960199828 1101 7 0.15905043848279377 1101 8 0.12828752843314817 1101 9 0.08941197309582094 1101 10-11 0.1685662953908036 1101 12-13 0.17353226155596957 1101 14-15 0.3553798014813552 1101 16-17 0.27107137864687303 1101 18-19 0.32748505433213637 1101 20-21 0.29362134659442773 1101 22-23 0.31608466754365594 1101 24-25 0.3515284168141619 1101 26-27 0.27669975689109805 1101 28-29 0.25043978214077356 1101 30-31 0.3119216689306512 1101 32-33 0.36443499526161816 1101 34-35 0.3081945319839292 1101 36-37 0.2783808927300129 1101 38-39 0.36093789905292795 1101 40-41 0.42337836294856857 1101 42-43 0.425433039161085 1101 44-45 0.4454376748709734 1101 46-47 0.418594432792986 1101 48-49 0.39156072943094955 1101 50-51 0.4975995121143697 1101 52-53 0.4252964447495984 1101 54-55 0.4871335732108264 1101 56-57 0.5237500282027696 1101 58-59 0.3869097545247371 1101 60-61 0.41933346707366326 1101 62-63 0.5375199176820118 1101 64-65 0.4405558028042549 1101 66-67 0.45916096755043156 1101 68-69 0.4680041044910581 1101 70-71 0.39851360855266194 1101 72-73 0.4044072149010418 1101 74-75 0.3266914558214893 1101 76-77 0.12185790509411021 1101 78-79 0.4236737465765259 1101 80-81 0.4047839396005273 1101 82-83 0.3354025884016494 1101 84-85 0.34747696771352565 1101 86-87 0.3430581409159643 1101 88-89 0.3688704707578694 1101 90-91 0.44189044036566116 1101 92-93 0.5952851155214915 1101 94-95 0.43203796940256467 1101 96-97 0.5584983385155198 1101 98-99 0.6137736236288269 1101 100-101 0.48869582503715847 2304 1 0.0443426787782073 2304 2 0.023166503957490647 2304 3 0.05498547928679898 2304 4 0.12193138254408353 2304 5 0.09732901995632659 2304 6 0.16242627684837885 2304 7 0.10462109065670688 2304 8 0.1459596298824195 2304 9 0.10780871222625876 2304 10-11 0.08160071568066485 2304 12-13 0.11301595720814106 2304 14-15 0.2202212869885969 2304 16-17 0.1897353279222287 2304 18-19 0.23033831520171333 2304 20-21 0.20424181760892424 2304 22-23 0.2532178197175696 2304 24-25 0.26501120666923583 2304 26-27 0.2742858076157333 2304 28-29 0.23872782561903705 2304 30-31 0.14437185009006726 2304 32-33 0.21383264018915327 2304 34-35 0.23479326386798505 2304 36-37 0.26732563910682217 2304 38-39 0.3199596381833629 2304 40-41 0.37048887019494714 2304 42-43 0.3420997058277564 2304 44-45 0.40330905168256237 2304 46-47 0.3477656284451598 2304 48-49 0.4454918888512367 2304 50-51 0.41350983820132825 2304 52-53 0.2897167346046672 2304 54-55 0.2718482471238701 2304 56-57 0.205384991970881 2304 58-59 0.2647720733653216 2304 60-61 0.267684916349026 2304 62-63 0.34208966405882535 2304 64-65 0.2783365999057068 2304 66-67 0.26652056899970944 2304 68-69 0.25024595231715097 2304 70-71 0.23633516652368058 2304 72-73 0.22614181635032082 2304 74-75 0.16856192683597726 2304 76-77 -0.022349522442119962 2304 78-79 0.35005508715624245 2304 80-81 0.35670422945560176 2304 82-83 0.39063356666251536 2304 84-85 0.43077406916280125 2304 86-87 0.3664548800463976 2304 88-89 0.2745362316274367 2304 90-91 0.2295942447134891 2304 92-93 0.3593747894345327 2304 94-95 0.17186586795329362 2304 96-97 0.2625381935879858 2304 98-99 0.47207978304910725 2304 100-101 0.36503640474730403 2202 1 0.062167770353298124 2202 2 0.07479981112651046 2202 3 0.053709969082717635 2202 4 0.09634464785020214 2202 5 0.1017301842682059 2202 6 0.07611315544596664 2202 7 0.12421390855309511 2202 8 0.1004713384119853 2202 9 0.10507813922782816 2202 10-11 0.15544261782613233 2202 12-13 0.10110563538710693 2202 14-15 0.12077090106500066 2202 16-17 0.06993630254284966 2202 18-19 0.12981339369463996 2202 20-21 0.15850087700191295 2202 22-23 0.18701736838319505 2202 24-25 0.17275666062004547 2202 26-27 0.1171558691018717 2202 28-29 0.15483163252641674 2202 30-31 0.12890498064998468 2202 32-33 0.1771331046056872 2202 34-35 0.24657979578844902 2202 36-37 0.15725417783000495 2202 38-39 0.23777753457268602 2202 40-41 0.21170322132001473 2202 42-43 0.21082942848604347 2202 44-45 0.21782403075111745 2202 46-47 0.26116780662412964 2202 48-49 0.3204532964912161 2202 50-51 0.20124482642738428 2202 52-53 0.30028057552565457 2202 54-55 0.3354427329951477 2202 56-57 0.3231657677427293 2202 58-59 0.3044711837787162 2202 60-61 0.3209377944233296 2202 62-63 0.28439555757007184 2202 64-65 0.22878629901611447 2202 66-67 0.23739265822001698 2202 68-69 0.35708710223865125 2202 70-71 0.23310698101870742 2202 72-73 0.3324230499976224 2202 74-75 0.26414162987103396 2202 76-77 0.2635523286829411 2202 78-79 0.3581446018082559 2202 80-81 0.2716244283043707 2202 82-83 0.3492820164270398 2202 84-85 0.3215956285557908 2202 86-87 0.2813891422128023 2202 88-89 0.2944641162428212 2202 90-91 0.3122840995015608 2202 92-93 0.4082881528568265 2202 94-95 0.23067441060111804 2202 96-97 0.30299018207568906 2202 98-99 0.3847854999512599 2202 100-101 0.4313995653961804 1102 1 0.03620651596564528 1102 2 -0.007296694012055127 1102 3 0.004658820140342357 1102 4 0.08907910768038363 1102 5 0.06437557475985756 1102 6 0.06786914674873401 1102 7 0.010924319647116931 1102 8 0.03833072877318955 1102 9 0.018950256689500122 1102 10-11 0.06431492417850393 1102 12-13 0.05354836461554413 1102 14-15 0.0650399206603467 1102 16-17 0.10425432820424163 1102 18-19 0.10757264026280922 1102 20-21 0.015071214582036419 1102 22-23 0.07022166207516278 1102 24-25 0.014412233647995265 1102 26-27 -0.018300263544254847 1102 28-29 0.02691533736936691 1102 30-31 0.04193207052623649 1102 32-33 0.14712455361272703 1102 34-35 0.07431508422895661 1102 36-37 -0.029973019005602453 1102 38-39 0.09097862671504231 1102 40-41 0.011670600783403984 1102 42-43 0.07452262366945206 1102 44-45 0.07063009417175437 1102 46-47 0.034077258920817144 1102 48-49 -0.00444254431289437 1102 50-51 -0.07828531831305696 1102 52-53 -0.02338253217480002 1102 54-55 0.048356813113727526 1102 56-57 0.01684138649993372 1102 58-59 -0.0193231513009664 1102 60-61 -0.11137435231049153 1102 62-63 -0.10948834637546412 1102 64-65 -0.16791093252520994 1102 66-67 -0.05491361740750733 1102 68-69 0.061218115787724514 1102 70-71 -0.08525894186135474 1102 72-73 -0.013418075076643277 1102 74-75 -0.002941116499229679 1102 76-77 -0.11458002864629435 1102 78-79 0.1372273117285303 1102 80-81 0.2017338402847031 1102 82-83 0.1870698681289582 1102 84-85 0.07429305769448291 1102 86-87 0.09983838728459915 1102 88-89 -0.06363077579877086 1102 90-91 -0.047961158087787226 1102 92-93 -0.11298638042821807 1102 94-95 -0.24438048944471547 1102 96-97 -0.006239537188470479 1102 98-99 0.009232090813728888 1102 100-101 0.019406117099247666 2305 1 -0.011612259056924756 2305 2 0.028576983599229777 2305 3 0.04184909958024008 2305 4 0.020977033949421298 2305 5 0.05427310840131838 2305 6 -0.0013667745333947323 2305 7 -0.06699051885363616 2305 8 0.02487863141444535 2305 9 0.044506277190016874 2305 10-11 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-0.15525907299734953 1213 100-101 0.01750632799974383 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 21.0 9 49.0 10 43.0 11 40.0 12 75.0 13 176.0 14 594.0 15 1381.0 16 2678.0 17 4472.0 18 6820.0 19 9154.0 20 12097.0 21 15618.0 22 20191.0 23 25921.0 24 32547.0 25 41254.0 26 52439.0 27 67564.0 28 88337.0 29 115432.0 30 131203.0 31 159558.0 32 214973.0 33 305164.0 34 464685.0 35 783336.0 36 1383819.0 37 1626151.0 38 250030.0 39 60.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.013762589262505 24.712846547655165 22.850132712589513 15.423258150492822 2 31.689621625748256 21.999277151771647 32.509944321428804 13.801156901051293 3 39.36522439760642 18.798146179719602 24.928566294845734 16.908063127828246 4 31.227937050001813 18.28293514178506 32.76809757411389 17.72103023409924 5 34.231402906730224 16.877508862800173 31.36703942755372 17.52404880291588 6 28.11413642849012 17.80629318132658 36.3602115723806 17.7193588178027 7 34.50895324217376 18.671097522267473 27.454081771260146 19.36586746429862 8 30.11833802680316 26.002986305430543 24.85815565033816 19.02052001742814 9 31.577136537501964 23.647453645036126 24.85887781079465 19.91653200666726 10-11 26.147607533990545 27.979350337575625 28.366411147956576 17.506630980477254 12-13 25.81066809814917 26.25611214257786 27.99355282655322 19.93966693271975 14-15 25.441669896328705 26.75813401991306 27.72862310480165 20.07157297895659 16-17 24.829707686641513 27.112646026862308 28.535929717968834 19.521716568527353 18-19 23.37076302442175 27.19296918335001 29.221062256765183 20.215205535463067 20-21 23.34030332802488 27.872685518722694 29.02546853598474 19.761542617267683 22-23 23.227414173808892 28.45470386091052 28.456603830682948 19.86127813459764 24-25 23.463887334715526 26.778328721249846 29.490849367301468 20.26693457673316 26-27 22.64721842705887 26.660556386804274 29.346443067448757 21.345782118688103 28-29 22.389467324130717 27.883793034315346 28.344746334261938 21.381993307292 30-31 20.95505032598667 28.448427942657712 28.822309324707756 21.774212406647866 32-33 20.97842597219132 28.09451773608887 28.880838710276446 22.046217581443365 34-35 21.99803916241767 28.10591755472343 29.0299734416895 20.866069841169406 36-37 21.33023847457703 29.321734863258918 29.33408896535384 20.013937696810217 38-39 21.254549181018458 28.574720051060183 28.822060007407302 21.348670760514054 40-41 21.731157888003917 27.815652036956735 27.782424058809994 22.67076601622935 42-43 22.348648064042564 28.431525949116576 28.355381006698554 20.86444498014231 44-45 21.264736801743915 28.605755756392583 28.297410435768818 21.832097006094692 46-47 22.04633692579627 28.36520010729245 28.427572439596418 21.16089052731487 48-49 22.094042052308367 28.29185828566555 28.74753113798881 20.86656852403728 50-51 21.780734662619015 28.288820583572853 27.593680784878575 22.336763968929553 52-53 21.116341650564525 28.39651892816689 27.85395661341317 22.63318280785541 54-55 22.056061941740076 28.46964719404409 28.277646233824346 21.196644630391486 56-57 21.955499792651764 28.265300498452607 27.574546699268016 22.204653009627613 58-59 21.379494052412102 27.936522376228666 28.192941517571057 22.491042053788178 60-61 21.10435134803554 27.87903475478074 28.91973430213559 22.09687959504813 62-63 20.82088224142867 27.912455046679234 28.806835913823743 22.45982679806835 64-65 21.904377943678607 28.434532811298567 28.27532407279632 21.385765172226506 66-67 22.15897775960229 28.70750968973918 27.749309792027876 21.384202758630654 68-69 21.778064036790973 27.857612129738396 28.69140265578806 21.672921177682575 70-71 21.36363296315249 29.263198554073604 28.433574295268677 20.93959418750523 72-73 22.3476397152573 29.503903225728788 27.722550247345882 20.42590681166803 74-75 23.3796101227073 28.49352927519283 27.432837746038025 20.694022856061846 76-77 23.408471665043283 27.19570635156478 27.87144918803207 21.52437279535987 78-79 23.054280013512162 27.39823040196963 28.628451045881427 20.91903853863678 80-81 22.19799844626993 28.44066267722341 28.235175169481412 21.12616370702525 82-83 21.305438624062603 28.451097226439625 28.578318614351204 21.665145535146564 84-85 21.538746308733142 27.941458665548787 28.766579535516808 21.75321549020127 86-87 20.727166168714636 28.587187119759307 29.25393008356256 21.431716627963496 88-89 20.713102084868147 29.118696264379086 29.14419048247958 21.02401116827319 90-91 21.407356031895596 28.213872179268 28.430928621165783 21.94784316767062 92-93 21.349667887997768 28.613662943399643 27.61771817768377 22.418950990918823 94-95 21.08589157989132 29.590258357385885 27.14730669456754 22.176543368155254 96-97 21.097770567354758 30.40227745722695 26.420166850381115 22.07978512503718 98-99 21.34021181515986 29.03467203736326 27.32033742857548 22.304778718901392 100-101 21.968392860336593 29.06011949883679 27.052054771905254 21.91943286892136 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 230.0 1 146.0 2 159.5 3 365.5 4 367.5 5 228.0 6 199.0 7 192.5 8 206.0 9 218.5 10 212.5 11 215.5 12 203.0 13 202.5 14 208.5 15 213.0 16 225.0 17 251.5 18 287.0 19 323.5 20 381.0 21 484.5 22 597.5 23 780.0 24 1096.5 25 1517.5 26 2124.0 27 2950.0 28 3942.5 29 5126.5 30 6900.5 31 9854.0 32 16121.0 33 28341.0 34 48146.0 35 82134.0 36 128579.5 37 195050.5 38 280707.5 39 335285.0 40 355396.5 41 347061.0 42 356597.5 43 401087.0 44 398943.0 45 380183.0 46 395043.5 47 379106.0 48 312999.5 49 254557.0 50 207017.5 51 171161.0 52 153200.5 53 139638.0 54 111542.0 55 70732.5 56 45221.5 57 35822.5 58 31614.0 59 27471.0 60 22337.0 61 17634.0 62 12646.5 63 8566.0 64 6102.0 65 4472.0 66 3594.5 67 3035.0 68 2403.0 69 1799.0 70 1287.0 71 865.5 72 645.0 73 502.0 74 324.5 75 196.0 76 130.5 77 96.0 78 60.0 79 36.0 80 28.0 81 24.0 82 15.5 83 8.5 84 7.5 85 5.0 86 2.0 87 0.5 88 2.0 89 1.5 90 0.0 91 0.5 92 0.5 93 0.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19314353351735816 2 0.0 3 0.0 4 0.026702742593470774 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 3.438859316609243E-5 48-49 0.0033786792785685817 50-51 2.5791444874569323E-5 52-53 0.0024845758562501786 54-55 0.009396683082634758 56-57 0.013506119965982805 58-59 0.013643674338647174 60-61 0.0030691819400737496 62-63 0.0021836756660468695 64-65 0.009637403234797405 66-67 0.016644079092388738 68-69 0.02427834677526126 70-71 0.02065894734453003 72-73 0.016300193160727814 74-75 0.02118337339031294 76-77 0.015027815213582393 78-79 0.006542429849849086 80-81 0.0034388593166092434 82-83 6.189946769896639E-4 84-85 0.0022008699626299157 86-87 0.01543188118328398 88-89 0.014408820536592728 90-91 0.017796096963452836 92-93 0.0180798028570731 94-95 0.017374836697168203 96-97 0.01979923251537772 98-99 0.027760191833328115 100-101 0.015990695822232982 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5815882.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.649393199139897 #Duplication Level Percentage of deduplicated Percentage of total 1 83.67663087522259 25.646379612739867 2 6.634551782888145 4.066899725875867 3 2.857760926865999 2.627659149499634 4 1.4553098179574862 1.7841745134859082 5 0.8217154486439242 1.2592539941647642 6 0.561749316339735 1.0330365405506736 7 0.481901013017562 1.0338981541727363 8 0.31229108558627283 0.7657225819775941 9 0.3454021582909231 0.9527729905161054 >10 2.167769560813989 13.424453836598579 >50 0.33577230611588343 7.1230343102288805 >100 0.29176319061002515 18.506355255319423 >500 0.03529513899105332 7.6381367582368025 >1k 0.021302967927120844 11.120584665627957 >5k 5.042765547569776E-4 1.0095233445929046 >10k+ 2.801341746228615E-4 2.0081145664124485 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT 43721 0.7517518409073637 TruSeq Adapter, Index 7 (97% over 38bp) GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 24474 0.42081321457347315 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 24447 0.4203489685657309 No Hit TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT 13034 0.22411046166342438 No Hit GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 11266 0.19371094530459868 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 8164 0.14037423730398932 No Hit TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 8159 0.1402882658210741 No Hit GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 7613 0.13090017988673083 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 7034 0.12094468216514709 No Hit TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 6179 0.10624355858664257 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 6141 0.10559017531648683 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 4.126631179931092E-4 0.0 0.0 2 4.814403043252941E-4 0.0 0.0 3 5.330231940744327E-4 0.0 0.0 4 6.018003804066177E-4 0.0 0.0 5 6.533832701557563E-4 0.0 0.0 6 8.081319394031722E-4 0.0 0.0 7 9.112977189014495E-4 0.0 0.0 8 0.0011348235744810503 0.0 0.0 9 0.0012723779471454201 0.0 0.0 10-11 0.0020977041831316385 0.0 0.0 12-13 0.0030863762366567958 0.0 0.0 14-15 0.004401739925259831 0.0 0.0 16-17 0.006060989545523792 0.0 0.0 18-19 0.007806210648702983 0.0 0.0 20-21 0.010093052094248128 0.0 0.0 22-23 0.012508850764166123 0.0 0.0 24-25 0.015302923958911134 0.0 0.0 26-27 0.01844948023360859 0.0 0.0 28-29 0.021690605139512806 0.0 0.0 30-31 0.03280671788045218 0.0 0.0 32-33 0.036718420353095194 0.0 0.0 34-35 0.04087084297790086 0.0 1.7194296583046217E-5 36-37 0.04518661142024546 0.0 1.7194296583046217E-5 38-39 0.050044000204956016 0.0 1.7194296583046217E-5 40-41 0.05615657264022894 0.0 1.7194296583046217E-5 42-43 0.0621659792960036 0.0 1.7194296583046217E-5 44-45 0.06890614355655772 0.0 1.7194296583046217E-5 46-47 0.07666077131551155 0.0 1.7194296583046217E-5 48-49 0.0856447912801532 0.0 1.7194296583046217E-5 50-51 0.09547133177736412 0.0 1.7194296583046217E-5 52-53 0.10678517892900853 0.0 1.7194296583046217E-5 54-55 0.11973248425604233 0.0 1.7194296583046217E-5 56-57 0.13461414794866883 0.0 1.7194296583046217E-5 58-59 0.15114646411326776 0.0 1.7194296583046217E-5 60-61 0.16880500670405624 0.0 1.7194296583046217E-5 62-63 0.18999697724266068 0.0 1.7194296583046217E-5 64-65 0.21322647192635613 0.0 1.7194296583046217E-5 66-67 0.240144143227115 0.0 1.7194296583046217E-5 68-69 0.2694260303080427 0.0 1.7194296583046217E-5 70-71 0.30344494609759964 0.0 1.7194296583046217E-5 72-73 0.34018056074727787 0.0 3.438859316609243E-5 74-75 0.38223781018940894 0.0 6.877718633218487E-5 76-77 0.42929000278891494 0.0 8.597148291523109E-5 78-79 0.48307376250068346 0.0 8.597148291523109E-5 80-81 0.5433741606174265 0.0 1.031657794982773E-4 82-83 0.6107242203332186 0.0 1.3755437266436973E-4 84-85 0.6854248418382629 0.0 1.3755437266436973E-4 86-87 0.7692040519391556 0.0 1.5474866924741597E-4 88-89 0.8607292926507106 0.0 1.5474866924741597E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGTGTT 63585 0.0 45.837044 1 CGGAAGA 11390 0.0 42.710857 4 GAAGAGC 11575 0.0 41.933155 6 TCGGAAG 11645 0.0 41.77559 3 GCCGTCT 5810 0.0 41.61211 50-51 TATGCCG 5795 0.0 41.309288 48-49 ATGCCGT 5860 0.0 41.09424 48-49 TGCCGTC 5885 0.0 41.041435 50-51 CGAAATG 220 0.0 41.0165 9 TCGTATG 5905 0.0 40.738926 44-45 CGTATGC 5880 0.0 40.669815 46-47 CTCGTAT 5850 0.0 40.350655 44-45 TCTCGTA 5905 0.0 39.974823 42-43 CATCTCG 5910 0.0 39.85858 40-41 GATCGGA 12295 0.0 39.527943 1 ATCTCGT 5955 0.0 39.43979 42-43 CGGACCG 110 1.5897967E-9 38.94546 1 CTGCTTG 6205 0.0 38.736336 58-59 CTTGAAA 5985 0.0 38.09944 62-63 GTCCGGC 125 1.3278623E-10 38.080006 1 >>END_MODULE