FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n01_34310-1.34100000035813.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n01_34310-1.34100000035813.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5815882
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT437210.7517518409073637TruSeq Adapter, Index 7 (97% over 38bp)
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC244740.42081321457347315No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA244470.4203489685657309No Hit
TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT130340.22411046166342438No Hit
GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC112660.19371094530459868No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC81640.14037423730398932No Hit
TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT81590.1402882658210741No Hit
GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC76130.13090017988673083No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA70340.12094468216514709No Hit
TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT61790.10624355858664257No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG61410.10559017531648683No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTGTT635850.045.8370441
CGGAAGA113900.042.7108574
GAAGAGC115750.041.9331556
TCGGAAG116450.041.775593
GCCGTCT58100.041.6121150-51
TATGCCG57950.041.30928848-49
ATGCCGT58600.041.0942448-49
TGCCGTC58850.041.04143550-51
CGAAATG2200.041.01659
TCGTATG59050.040.73892644-45
CGTATGC58800.040.66981546-47
CTCGTAT58500.040.35065544-45
TCTCGTA59050.039.97482342-43
CATCTCG59100.039.8585840-41
GATCGGA122950.039.5279431
ATCTCGT59550.039.4397942-43
CGGACCG1101.5897967E-938.945461
CTGCTTG62050.038.73633658-59
CTTGAAA59850.038.0994462-63
GTCCGGC1251.3278623E-1038.0800061
Produced by FastQC (version 0.11.2)