##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n01_34302-1.341000000358ea.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8416479 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.67697406480786 33.0 33.0 33.0 33.0 33.0 2 32.70568999221646 33.0 33.0 33.0 33.0 33.0 3 32.74175317255589 33.0 33.0 33.0 33.0 33.0 4 36.663804899887474 37.0 37.0 37.0 37.0 37.0 5 36.665930848279906 37.0 37.0 37.0 37.0 37.0 6 36.53582038284656 37.0 37.0 37.0 37.0 37.0 7 36.427475788866104 37.0 37.0 37.0 37.0 37.0 8 36.36502223792158 37.0 37.0 37.0 37.0 37.0 9 36.42388842174976 37.0 37.0 37.0 37.0 37.0 10-11 36.355461886140276 37.0 37.0 37.0 37.0 37.0 12-13 36.332628703760804 37.0 37.0 37.0 37.0 37.0 14-15 38.15491430561402 40.0 37.0 40.0 37.0 40.0 16-17 38.16685391836658 40.0 37.0 40.0 37.0 40.0 18-19 38.1416373165073 40.0 37.0 40.0 37.0 40.0 20-21 38.13345087654826 40.0 37.0 40.0 37.0 40.0 22-23 37.96057240801052 40.0 37.0 40.0 33.0 40.0 24-25 37.989819673999065 40.0 37.0 40.0 33.0 40.0 26-27 37.91920196081996 40.0 37.0 40.0 33.0 40.0 28-29 37.74786635836672 40.0 37.0 40.0 33.0 40.0 30-31 37.74748306269166 40.0 37.0 40.0 33.0 40.0 32-33 37.616234829315204 40.0 37.0 40.0 33.0 40.0 34-35 37.44714357393394 40.0 37.0 40.0 33.0 40.0 36-37 37.165300477788875 40.0 37.0 40.0 33.0 40.0 38-39 37.32022185286745 40.0 37.0 40.0 33.0 40.0 40-41 37.10732136324465 38.5 37.0 40.0 33.0 40.0 42-43 36.911763339515254 37.0 37.0 40.0 33.0 40.0 44-45 36.915323735733196 37.0 37.0 40.0 33.0 40.0 46-47 36.927962987848005 37.0 37.0 40.0 33.0 40.0 48-49 36.60930681345489 37.0 37.0 40.0 33.0 40.0 50-51 36.5011676497975 37.0 37.0 40.0 33.0 40.0 52-53 36.413570508522625 37.0 37.0 40.0 33.0 40.0 54-55 36.15686120050914 37.0 37.0 40.0 33.0 40.0 56-57 36.03458821675905 37.0 37.0 40.0 33.0 40.0 58-59 35.74791798328018 37.0 37.0 40.0 27.0 40.0 60-61 35.46335557897786 37.0 33.0 40.0 27.0 40.0 62-63 35.2274298432872 37.0 33.0 38.5 27.0 40.0 64-65 34.965359980105696 37.0 33.0 37.0 27.0 40.0 66-67 34.3915860183338 37.0 33.0 37.0 27.0 40.0 68-69 34.20931627109151 37.0 33.0 37.0 27.0 40.0 70-71 33.75559797630339 37.0 33.0 37.0 27.0 38.5 72-73 33.012318096439145 37.0 33.0 37.0 22.0 37.0 74-75 32.31472050248091 37.0 33.0 37.0 22.0 37.0 76-77 29.820805291618978 33.0 27.0 35.0 18.5 37.0 78-79 31.9306170074208 33.0 33.0 37.0 22.0 37.0 80-81 32.24635474050372 37.0 33.0 37.0 22.0 37.0 82-83 32.137277476721565 37.0 33.0 37.0 22.0 37.0 84-85 31.95327036400851 35.0 33.0 37.0 15.0 37.0 86-87 31.84885104566886 33.0 33.0 37.0 15.0 37.0 88-89 31.725462690514643 33.0 33.0 37.0 15.0 37.0 90-91 31.574677130424732 33.0 33.0 37.0 15.0 37.0 92-93 31.42067377581528 33.0 33.0 37.0 15.0 37.0 94-95 31.288038976869068 33.0 33.0 37.0 15.0 37.0 96-97 31.124740286288365 33.0 33.0 37.0 15.0 37.0 98-99 30.95207889189767 33.0 33.0 37.0 15.0 37.0 100-101 29.34445900714539 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.08999708700385867 2306 2 0.09085361679711212 2306 3 0.11193301217703322 2306 4 0.14673282387530406 2306 5 0.15966279844274567 2306 6 0.19301261783919443 2306 7 0.2013453638542657 2306 8 0.2445622111361203 2306 9 0.23827121177296107 2306 10-11 0.2681697409824437 2306 12-13 0.2208391216308101 2306 14-15 0.4392188785071127 2306 16-17 0.462961590556894 2306 18-19 0.3813606928009463 2306 20-21 0.4612940524508815 2306 22-23 0.4877514936847902 2306 24-25 0.4471433571401917 2306 26-27 0.5668518872205155 2306 28-29 0.49215413038120204 2306 30-31 0.5015412023608192 2306 32-33 0.5887860291400813 2306 34-35 0.5239002822794134 2306 36-37 0.5475938657560562 2306 38-39 0.5949278054122047 2306 40-41 0.6278926126338717 2306 42-43 0.7121960174320705 2306 44-45 0.6073719480376312 2306 46-47 0.5952467600005491 2306 48-49 0.685465269223478 2306 50-51 0.6042441018767732 2306 52-53 0.6449728360481828 2306 54-55 0.6726497016347253 2306 56-57 0.5964646769781368 2306 58-59 0.6824768283669087 2306 60-61 0.6689078665725461 2306 62-63 0.6039549652232168 2306 64-65 0.5931464529518706 2306 66-67 0.5589819745138556 2306 68-69 0.7454903947407274 2306 70-71 0.752869900493728 2306 72-73 0.48849818877258144 2306 74-75 0.5229158205678246 2306 76-77 0.5816115606133927 2306 78-79 0.7479114328615815 2306 80-81 0.6778222064910793 2306 82-83 0.6423424479801696 2306 84-85 0.7648831955258188 2306 86-87 0.763428576932764 2306 88-89 0.7943359209289618 2306 90-91 0.7161072935189488 2306 92-93 0.8021353878619237 2306 94-95 0.6893634762613452 2306 96-97 0.7799896612472352 2306 98-99 0.8009083393661811 2306 100-101 0.6940746806660805 2307 1 0.12808942714979565 2307 2 0.1104365831522216 2307 3 0.116346848865021 2307 4 0.16894234282794685 2307 5 0.17607366436774896 2307 6 0.18367647420522815 2307 7 0.20721219094185983 2307 8 0.2421214606860147 2307 9 0.15302274748881928 2307 10-11 0.23049812256081026 2307 12-13 0.20267885184692602 2307 14-15 0.5093162081760525 2307 16-17 0.4885747764816628 2307 18-19 0.44900423531669986 2307 20-21 0.4510230335052796 2307 22-23 0.46714956722515666 2307 24-25 0.5314801584350448 2307 26-27 0.4985205065013574 2307 28-29 0.4346840192561885 2307 30-31 0.5229390020656908 2307 32-33 0.5533871080975032 2307 34-35 0.5421034683201853 2307 36-37 0.5120826763033222 2307 38-39 0.5483477171261484 2307 40-41 0.5811677612755801 2307 42-43 0.5382995646374127 2307 44-45 0.6671154038457203 2307 46-47 0.6514069608077193 2307 48-49 0.6054985857027404 2307 50-51 0.5804459952773016 2307 52-53 0.5576346283947089 2307 54-55 0.4775668845220338 2307 56-57 0.5509774709163082 2307 58-59 0.5364460888826486 2307 60-61 0.5113213391131879 2307 62-63 0.5595665577443967 2307 64-65 0.46960458294542917 2307 66-67 0.6141232411086577 2307 68-69 0.7219466915692863 2307 70-71 0.5860256826482768 2307 72-73 0.40153471836545407 2307 74-75 0.2239085234318665 2307 76-77 0.344502037704423 2307 78-79 0.38261714825577897 2307 80-81 0.4074481310483691 2307 82-83 0.3637577999809878 2307 84-85 0.4500447967881982 2307 86-87 0.40820434926485305 2307 88-89 0.33087755365963645 2307 90-91 0.3086811032666681 2307 92-93 0.49167066707055795 2307 94-95 0.33088712843972345 2307 96-97 0.33292044932590414 2307 98-99 0.4446368902999964 2307 100-101 0.239946747809217 2201 1 0.09806293782898479 2201 2 0.11556438558719861 2201 3 0.07937938306135806 2201 4 0.16324386797212043 2201 5 0.1254181414622053 2201 6 0.17983867331430048 2201 7 0.1819570261977148 2201 8 0.2252018218874099 2201 9 0.19547603282358494 2201 10-11 0.22947909902442376 2201 12-13 0.2321200339623246 2201 14-15 0.45757252831057826 2201 16-17 0.4114760978692331 2201 18-19 0.386573170322869 2201 20-21 0.41321070651920877 2201 22-23 0.48810139581562595 2201 24-25 0.4347114876005733 2201 26-27 0.44760468405051057 2201 28-29 0.5048177429466492 2201 30-31 0.5193987430600728 2201 32-33 0.48783016375393373 2201 34-35 0.49608112339718957 2201 36-37 0.4847648006054399 2201 38-39 0.5344404388882324 2201 40-41 0.5428376010168776 2201 42-43 0.48624592075643847 2201 44-45 0.5510747540179892 2201 46-47 0.5222757547331156 2201 48-49 0.4786356630076014 2201 50-51 0.5667208281136666 2201 52-53 0.5288549096808026 2201 54-55 0.5662378296284842 2201 56-57 0.5893644386707209 2201 58-59 0.5198797051631701 2201 60-61 0.5483115808264287 2201 62-63 0.6101232086707569 2201 64-65 0.43157068989071234 2201 66-67 0.6924612827460024 2201 68-69 0.6188835602112164 2201 70-71 0.4655069570987038 2201 72-73 0.4113794309152041 2201 74-75 0.47330098145517496 2201 76-77 0.26541396636460135 2201 78-79 0.5467034350417599 2201 80-81 0.6530754329905477 2201 82-83 0.6125745590099356 2201 84-85 0.6668878224294943 2201 86-87 0.7142055128324394 2201 88-89 0.6425065434722832 2201 90-91 0.7021264754760175 2201 92-93 0.6013723267056363 2201 94-95 0.5986602505575576 2201 96-97 0.6637839112352992 2201 98-99 0.6877094302492317 2201 100-101 0.6832051868008087 1101 1 0.08516381400720974 1101 2 0.06564986639022408 1101 3 0.08379374242336013 1101 4 0.1076679234004203 1101 5 0.10899823291254052 1101 6 0.12230099729713828 1101 7 0.15781443927310335 1101 8 0.14304161249476266 1101 9 0.13729221730670105 1101 10-11 0.16940697901220148 1101 12-13 0.1799355049279967 1101 14-15 0.2934057630348619 1101 16-17 0.24234288027643203 1101 18-19 0.2780790012086385 1101 20-21 0.31556350380784437 1101 22-23 0.317011656867642 1101 24-25 0.32008826543349755 1101 26-27 0.2803969921911502 1101 28-29 0.2847712583446693 1101 30-31 0.33665434125790483 1101 32-33 0.3956174384175313 1101 34-35 0.35173323242407406 1101 36-37 0.3727133761314363 1101 38-39 0.3557446304500047 1101 40-41 0.3857036628421042 1101 42-43 0.39930390817804096 1101 44-45 0.36656015781764495 1101 46-47 0.42479726180912536 1101 48-49 0.4694357915820291 1101 50-51 0.5019775972572162 1101 52-53 0.44022045667846044 1101 54-55 0.45855087127918637 1101 56-57 0.4199766772610687 1101 58-59 0.4238788026695701 1101 60-61 0.3807367983697745 1101 62-63 0.5582405116454581 1101 64-65 0.40458953099003025 1101 66-67 0.49250764371871725 1101 68-69 0.5057332923772577 1101 70-71 0.41270331038874275 1101 72-73 0.21815162104287822 1101 74-75 0.23836975464669763 1101 76-77 0.06675613860135243 1101 78-79 0.32078438394931297 1101 80-81 0.3660625985071633 1101 82-83 0.2925899773448677 1101 84-85 0.37500757149742014 1101 86-87 0.36682349488500776 1101 88-89 0.27896061785117965 1101 90-91 0.3738727299509499 1101 92-93 0.49136391991591566 1101 94-95 0.29956742441032347 1101 96-97 0.3393881050930432 1101 98-99 0.4833333076513924 1101 100-101 0.31753623770250883 2304 1 0.061369382776312875 2304 2 0.04500449792755745 2304 3 0.06655202921847803 2304 4 0.11866022963482692 2304 5 0.10544116987917818 2304 6 0.10082717012622311 2304 7 0.0690746550803425 2304 8 0.07701669257350119 2304 9 0.09701219311798326 2304 10-11 0.13387768349522133 2304 12-13 0.11724266144832995 2304 14-15 0.19795443423952008 2304 16-17 0.24856938648230908 2304 18-19 0.23712163702364109 2304 20-21 0.22976042615576375 2304 22-23 0.21947831331387846 2304 24-25 0.24947977925485532 2304 26-27 0.2774506229660787 2304 28-29 0.2344126390559822 2304 30-31 0.18231376214021822 2304 32-33 0.2404999956799756 2304 34-35 0.21173646263717671 2304 36-37 0.2719111991144558 2304 38-39 0.297114206372008 2304 40-41 0.2646384821409953 2304 42-43 0.2391561671515987 2304 44-45 0.32235756993604525 2304 46-47 0.3370900883861907 2304 48-49 0.401013918213998 2304 50-51 0.33525664804695765 2304 52-53 0.18573642417327818 2304 54-55 0.271586648897582 2304 56-57 0.23780675678839458 2304 58-59 0.24373465283953522 2304 60-61 0.24877744743963603 2304 62-63 0.20304552029487155 2304 64-65 0.20386037915331912 2304 66-67 0.1908304528964564 2304 68-69 0.32745044498308573 2304 70-71 0.2417747280386493 2304 72-73 0.12444566272781543 2304 74-75 0.16292162081693817 2304 76-77 0.014663847365373073 2304 78-79 0.21016380543771973 2304 80-81 0.22283986962086288 2304 82-83 0.2529745108592323 2304 84-85 0.2557972109281721 2304 86-87 0.16678095071356935 2304 88-89 0.1344387137003089 2304 90-91 0.17019024041237785 2304 92-93 0.2922782954865113 2304 94-95 0.004922216708795446 2304 96-97 0.26715775658250607 2304 98-99 0.282350120398128 2304 100-101 0.14728811495748673 2202 1 0.07043566403425672 2202 2 0.07030015185871008 2202 3 0.07608814759513649 2202 4 0.09047315277832979 2202 5 0.08917095558219046 2202 6 0.06940913124033443 2202 7 0.09459843306065352 2202 8 0.10918141923818325 2202 9 0.11734468569048317 2202 10-11 0.13473167606965575 2202 12-13 0.10698693081022981 2202 14-15 0.1780091541863058 2202 16-17 0.08225915354543645 2202 18-19 0.1371905905838844 2202 20-21 0.20148894451951094 2202 22-23 0.18370729550314735 2202 24-25 0.14634028866864668 2202 26-27 0.13222456606976607 2202 28-29 0.1171924254757215 2202 30-31 0.12168562057632215 2202 32-33 0.21180438375361632 2202 34-35 0.19199762757860128 2202 36-37 0.16193439011059496 2202 38-39 0.1792571356141579 2202 40-41 0.25951030459136604 2202 42-43 0.26150840943596165 2202 44-45 0.2369322901713815 2202 46-47 0.26633052123581535 2202 48-49 0.3352684945496307 2202 50-51 0.2220578338371837 2202 52-53 0.3236318196968142 2202 54-55 0.25009125120427456 2202 56-57 0.31341739917979083 2202 58-59 0.21554532046661734 2202 60-61 0.14667440392965858 2202 62-63 0.30971630144600937 2202 64-65 0.23627651201598354 2202 66-67 0.2618835354315294 2202 68-69 0.26769268110877675 2202 70-71 0.13430925016211148 2202 72-73 -0.02301397056510268 2202 74-75 0.05998124013684247 2202 76-77 0.13580975931362715 2202 78-79 0.2576011743602926 2202 80-81 0.16533389404020227 2202 82-83 0.150957652309323 2202 84-85 0.24597410138786557 2202 86-87 0.1330363279998572 2202 88-89 0.10018165070442464 2202 90-91 0.035666133592688 2202 92-93 0.1396867658379648 2202 94-95 -0.12181471967097224 2202 96-97 0.12214628131650684 2202 98-99 0.17380770799358203 2202 100-101 0.22085459772997496 1102 1 0.06942014977472866 1102 2 0.05097814549679214 1102 3 0.04227193238465077 1102 4 0.12281704665988258 1102 5 0.11095535635624998 1102 6 0.12980189662290798 1102 7 0.11729423054563881 1102 8 0.09932865932673707 1102 9 0.09044291840554308 1102 10-11 0.08637177459318934 1102 12-13 0.11403728505956678 1102 14-15 0.11060810643978414 1102 16-17 0.10146692005441338 1102 18-19 0.07175867803558589 1102 20-21 0.09207472244153792 1102 22-23 0.11476775905695291 1102 24-25 0.14019580448748314 1102 26-27 0.11565107002371633 1102 28-29 0.14379140383052658 1102 30-31 0.16667739298792128 1102 32-33 0.18279340245832998 1102 34-35 0.09633180533442243 1102 36-37 0.06538699640677237 1102 38-39 0.0931796661658737 1102 40-41 0.13517233588022037 1102 42-43 0.13207615408347806 1102 44-45 0.16987365729895032 1102 46-47 0.15791760325933524 1102 48-49 0.11952730243975651 1102 50-51 0.011009760119321754 1102 52-53 0.10331120738609911 1102 54-55 0.166262482135906 1102 56-57 0.15860435433258857 1102 58-59 0.14383143474536553 1102 60-61 0.0014582684416311054 1102 62-63 0.05222507260046427 1102 64-65 0.05410146511103875 1102 66-67 0.08984474913102503 1102 68-69 0.03648379545887792 1102 70-71 -0.08772857384240496 1102 72-73 -0.12873526963862503 1102 74-75 -0.08546074291940187 1102 76-77 -0.09377896519765372 1102 78-79 0.13684691609897115 1102 80-81 0.17125155735465114 1102 82-83 0.09422391375317574 1102 84-85 0.15522683039412044 1102 86-87 0.12911903853359163 1102 88-89 0.05280944407745025 1102 90-91 0.09844836370797694 1102 92-93 0.13555073811422602 1102 94-95 -0.05926729147006071 1102 96-97 0.0594838769411794 1102 98-99 0.13710705075303764 1102 100-101 0.13061731822080347 2305 1 0.05314150836182563 2305 2 0.04926902427919089 2305 3 0.04292193923861731 2305 4 0.05502299863537985 2305 5 0.060962312940937124 2305 6 -0.005085876282322488 2305 7 -0.020430022129950487 2305 8 0.0691052531340901 2305 9 0.06984573681436501 2305 10-11 0.06636537403770859 2305 12-13 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16709.0 21 23028.0 22 30486.0 23 40335.0 24 52309.0 25 68114.0 26 92796.0 27 137568.0 28 226493.0 29 295113.0 30 216522.0 31 194375.0 32 255528.0 33 362389.0 34 553812.0 35 948333.0 36 1784929.0 37 2512442.0 38 572622.0 39 161.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 38.04981393371657 24.276183051463242 23.31824018591272 14.355762828907467 2 27.47018082026938 27.102853818087112 32.516756710258534 12.91020865138498 3 34.80519585446598 18.310733027433443 31.011519187536734 15.872551930563839 4 26.412839631767977 18.326662118831603 33.47777463496493 21.782723614435497 5 35.65008598013492 17.34058862381763 31.494512135062656 15.51481326098479 6 29.205137826059786 18.4610474832822 36.794571315935244 15.539243374722775 7 30.2772216267634 24.77885348493117 28.41542169831351 16.52850318999192 8 26.366120559440592 31.574818876159494 25.805185279972775 16.25387528442713 9 34.39568969399199 23.318729839401964 24.607796205515395 17.677784261090654 10-11 26.17642128020518 29.41784206911227 27.47781465384753 16.927921996835018 12-13 23.066878679314712 27.83685434253445 27.133513907656635 21.962753070494205 14-15 22.286706828354234 28.461064300166377 27.588086419511054 21.664142451968335 16-17 24.435515136436507 26.25780923352865 28.44864224101314 20.858033389021706 18-19 20.356142990435785 26.477319078441237 31.805925019239044 21.360612911883937 20-21 23.608156094727974 26.745287429577143 31.405104200937235 18.241452274757652 22-23 20.634388798451226 33.07087203568143 28.049674929385553 18.245064236481788 24-25 20.73481083954466 26.107081120264187 31.576304057789486 21.581803982401667 26-27 20.4934034766795 26.201764419539337 28.280519680498223 25.02431242328294 28-29 23.13390195591292 29.73270651539676 27.95574016165192 19.1776513670384 30-31 19.412589278723324 27.10889553695791 31.154352075256174 22.32416310906259 32-33 19.385101537115464 30.122388471473645 28.458123640538997 22.034386350871902 34-35 22.852673903184456 30.333973387208594 28.527796481165108 18.285556228441845 36-37 19.368681369014286 30.86242477406526 31.770346008110995 17.99854784880946 38-39 19.92849979189635 27.31175946616156 30.811702851037825 21.94803789090426 40-41 23.14762503417403 26.899473045676224 30.70005283682167 19.252849083328076 42-43 20.08170518811964 30.22760467886868 31.125949461764236 18.564740671247442 44-45 19.516165845598856 27.40808240595622 31.320650832729456 21.755100915715467 46-47 22.985490655378484 27.24014722319832 28.136603547388766 21.63775857403443 48-49 20.230220672404343 30.520790533859397 30.624810335703135 18.62417845803313 50-51 23.179307952146207 27.518484590333003 30.085403975776064 19.21680348174472 52-53 19.59268664568146 27.349171943909685 27.356306952712593 25.70183445769626 54-55 22.98811956342467 27.274586355666397 30.470630788893686 19.26666329201524 56-57 19.839603094929814 27.02769071425991 30.130867399806448 23.001838791003827 58-59 19.780793958227648 26.80152969378674 30.733562131451613 22.684114216533995 60-61 22.614683796259246 27.040501851460082 30.9192912640872 19.425523088193472 62-63 19.62015435072497 27.160682614554375 30.74625425677618 22.47290877794448 64-65 23.054685579278246 27.224782870992613 30.473331789378772 19.247199760350366 66-67 20.18563246767831 33.337860954496215 26.972227619320936 19.504278958504532 68-69 20.160926791139346 33.22086637273136 27.02557888495604 19.592627951173256 70-71 19.765968521678783 34.22977463195273 26.981715288044228 19.022541558324264 72-73 19.72902081541526 34.312663490804404 26.8055489948797 19.152766698900628 74-75 20.549625656050406 33.17102324284378 26.731994759856153 19.54735634124966 76-77 20.78766342934775 31.93944725237867 27.338705197208483 19.93418412106509 78-79 20.634197797444898 31.624521776172088 27.870149475858664 19.87113095052435 80-81 20.06928440630336 31.942290441880427 27.67072636699348 20.31769878482273 82-83 19.323162342183494 31.63006955451368 28.07937935606826 20.967388747234573 84-85 19.463371772837206 31.155294130227755 28.308107347032617 21.07322674990242 86-87 19.38075652607811 31.157262941967694 28.831533419059944 20.630447112894252 88-89 19.562436974330563 31.423514436101623 28.514886475959873 20.499162113607934 90-91 20.075496833155622 30.718090392395176 28.10476269983473 21.101650074614472 92-93 19.66418319060907 31.471456147561366 27.785066118843805 21.07929454298576 94-95 19.448662455442513 31.953594141489717 27.605599876030485 20.992143527037285 96-97 19.925455075149515 32.268767443614024 27.048905489815166 20.756871991421296 98-99 20.218046365604707 31.22616347214111 27.04812004446831 21.507670117785874 100-101 20.243443044713615 31.383784644526337 26.74707757830854 21.625694732451507 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 121.0 1 88.0 2 151.5 3 421.0 4 466.5 5 289.0 6 233.0 7 231.5 8 224.5 9 224.0 10 226.0 11 224.5 12 229.0 13 248.0 14 268.5 15 284.0 16 326.0 17 438.0 18 591.0 19 860.5 20 1316.0 21 1917.0 22 2955.5 23 4526.0 24 6399.5 25 8995.5 26 12698.0 27 17611.0 28 23302.0 29 29368.5 30 36358.5 31 44432.5 32 55837.5 33 81373.5 34 125082.0 35 207478.0 36 321994.5 37 444615.5 38 582825.0 39 653088.0 40 655912.5 41 611164.0 42 548235.0 43 539434.0 44 514751.0 45 473011.5 46 462008.5 47 409974.0 48 312709.5 49 252040.5 50 208192.5 51 170901.5 52 148218.0 53 123321.5 54 91238.5 55 61056.5 56 42460.0 57 30952.0 58 23765.0 59 18242.5 60 13611.5 61 10193.0 62 7363.5 63 5217.5 64 3915.5 65 2994.5 66 2180.5 67 1563.0 68 1158.0 69 776.0 70 484.0 71 332.0 72 245.0 73 163.0 74 109.5 75 87.0 76 63.0 77 50.5 78 33.5 79 23.5 80 20.5 81 12.5 82 7.0 83 6.5 84 5.0 85 3.0 86 3.5 87 2.0 88 1.5 89 1.0 90 1.0 91 1.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18984185667189332 2 0.0 3 0.0 4 0.026364944295589637 5 0.0 6 1.1881453039923227E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 7.722944475950097E-5 48-49 0.0034812657406975054 50-51 1.0693307735930904E-4 52-53 0.002750556378742227 54-55 0.009445755166738965 56-57 0.013865655697590407 58-59 0.014257743647907873 60-61 0.003368391936818235 62-63 0.0022812389836652596 64-65 0.010218049614333976 66-67 0.017394447250447604 68-69 0.025616412754074475 70-71 0.021374734018821884 72-73 0.017127114557049333 74-75 0.02167771107133993 76-77 0.01587956198785739 78-79 0.006790250412316124 80-81 0.003558495185457006 82-83 7.010057293554704E-4 84-85 0.0026614454809428026 86-87 0.016111250322135895 88-89 0.014934986471183498 90-91 0.0184934816566405 92-93 0.01893309541911766 94-95 0.017905349731164304 96-97 0.020049952004870447 98-99 0.028479842936695976 100-101 0.016265709211654898 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8416479.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.73346185997407 #Duplication Level Percentage of deduplicated Percentage of total 1 89.32514759120374 31.0257160699563 2 4.666824997522169 3.241899761172198 3 1.5797982817777663 1.6461559009974185 4 0.9833322965108033 1.3661813926615483 5 0.45777512166519374 0.79500573644015 6 0.40033415250757726 0.8342994610420188 7 0.3353696313516219 0.8153983809681588 8 0.24818632096328866 0.6896296090676536 9 0.20209113543307408 0.631739227032318 >10 1.3986654355788273 9.725880196844713 >50 0.17464628145695224 4.191227294891671 >100 0.16964047953826794 13.132464782403689 >500 0.031193821249004043 7.512969683100974 >1k 0.02613708282401463 16.420745300670045 >5k 6.174732751158325E-4 1.309997343973404 >10k+ 2.39897142539789E-4 6.660689858777892 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT 482461 5.7323377150944 TruSeq Adapter, Index 7 (97% over 35bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 16355 0.19432116446794437 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 14042 0.16683936358660195 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 13564 0.16116002903351867 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 11594 0.1377535665448699 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 10958 0.1301969624114787 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 10583 0.12574141752150753 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 9906 0.11769767381347948 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 9020 0.10717070642010751 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0015921147073497124 0.0 1.1881453039923227E-5 2 0.0017703365029485608 0.0 1.1881453039923227E-5 3 0.0021148986411063343 0.0 1.1881453039923227E-5 4 0.0022337131715055667 0.0 1.1881453039923227E-5 5 0.002566393856623417 0.0 1.1881453039923227E-5 6 0.0029941261660606532 0.0 1.1881453039923227E-5 7 0.0036832504423762002 0.0 2.3762906079846454E-5 8 0.004336730359571978 0.0 2.3762906079846454E-5 9 0.005037736088927448 0.0 2.3762906079846454E-5 10-11 0.00791304772458887 0.0 2.3762906079846454E-5 12-13 0.01067548555637102 0.0 2.3762906079846454E-5 14-15 0.013966648048429753 0.0 2.9703632599808068E-5 16-17 0.01660433062329271 0.0 3.564435911976968E-5 18-19 0.020133122176149907 0.0 3.564435911976968E-5 20-21 0.024238164201443382 0.0 3.564435911976968E-5 22-23 0.029240255931251062 0.0 3.564435911976968E-5 24-25 0.03466413924397602 0.0 4.752581215969291E-5 26-27 0.04057516213133782 0.0 4.752581215969291E-5 28-29 0.04719313147457506 0.0 5.9407265199616135E-5 30-31 0.0717461541815764 0.0 6.534799171957775E-5 32-33 0.0793740470332071 0.0 7.128871823953936E-5 34-35 0.08645539304500136 0.0 7.128871823953936E-5 36-37 0.09312682892691826 0.0 8.317017127946258E-5 38-39 0.10035669310171152 0.0 8.317017127946258E-5 40-41 0.10845390334841921 0.0 8.317017127946258E-5 42-43 0.11670557248464589 0.0 8.317017127946258E-5 44-45 0.12605627602706548 0.0 8.317017127946258E-5 46-47 0.13601293367452114 0.0 1.0099235083934743E-4 48-49 0.14607058367281617 0.0 1.0693307735930904E-4 50-51 0.15710251282038484 0.0 1.0693307735930904E-4 52-53 0.16842553756743173 0.0 1.0693307735930904E-4 54-55 0.18125750685054878 0.0 1.0693307735930904E-4 56-57 0.1952122734459386 0.0 1.1881453039923227E-4 58-59 0.20977893487288451 0.0 1.1881453039923227E-4 60-61 0.22544463070602327 0.0 1.1881453039923227E-4 62-63 0.24193608752543672 0.0 1.1881453039923227E-4 64-65 0.2597048005466419 0.0 1.1881453039923227E-4 66-67 0.2799389150736311 0.0 1.1881453039923227E-4 68-69 0.3025909052942448 0.0 1.4257743647907872E-4 70-71 0.32687659530784785 0.0 1.5445888951900194E-4 72-73 0.35314054725259814 0.0 1.6039961603896355E-4 74-75 0.3827372467750469 0.0 1.722810690788868E-4 76-77 0.4146330074607208 0.0 1.9010324863877163E-4 78-79 0.45097837230984594 0.0 1.9010324863877163E-4 80-81 0.49274167974517613 0.0 1.9010324863877163E-4 82-83 0.5396853007059128 0.0 1.9604397515873324E-4 84-85 0.5926706405374504 0.0 2.0198470167869485E-4 86-87 0.6508125309883147 0.0 2.0198470167869485E-4 88-89 0.7173308458323249 0.0 2.1386615471861807E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 62510 0.0 81.67692 3 CGGAAGA 62475 0.0 81.54018 4 GATCGGA 62845 0.0 81.38505 1 ATCGGAA 63555 0.0 80.34143 2 GAAGAGC 63555 0.0 80.19005 6 GGAAGAG 66640 0.0 76.61318 5 GAGCACA 92290 0.0 55.22758 9 AGAGCAC 92330 0.0 55.224228 8 AAGAGCA 93210 0.0 54.616234 7 CGCGCGG 40 5.3344155E-4 47.60022 1 TTGTGTT 94975 0.0 46.735672 1 GCCGTCT 55485 0.0 45.73187 50-51 ATGCCGT 55485 0.0 45.64504 48-49 TCGTATG 55875 0.0 45.548737 44-45 CGTCTTC 55730 0.0 45.51621 52-53 TCTCGTA 55630 0.0 45.399292 42-43 CGTATCT 55115 0.0 45.35601 38-39 TATGCCG 55435 0.0 45.352047 48-49 TGCCGTC 55515 0.0 45.31785 50-51 CGTATGC 55660 0.0 45.234295 46-47 >>END_MODULE