FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n01_34302-1.341000000358ea.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n01_34302-1.341000000358ea.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8416479
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT4824615.7323377150944TruSeq Adapter, Index 7 (97% over 35bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC163550.19432116446794437No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA140420.16683936358660195No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG135640.16116002903351867No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA115940.1377535665448699No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC109580.1301969624114787No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC105830.12574141752150753No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA99060.11769767381347948No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG90200.10717070642010751No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG625100.081.676923
CGGAAGA624750.081.540184
GATCGGA628450.081.385051
ATCGGAA635550.080.341432
GAAGAGC635550.080.190056
GGAAGAG666400.076.613185
GAGCACA922900.055.227589
AGAGCAC923300.055.2242288
AAGAGCA932100.054.6162347
CGCGCGG405.3344155E-447.600221
TTGTGTT949750.046.7356721
GCCGTCT554850.045.7318750-51
ATGCCGT554850.045.6450448-49
TCGTATG558750.045.54873744-45
CGTCTTC557300.045.5162152-53
TCTCGTA556300.045.39929242-43
CGTATCT551150.045.3560138-39
TATGCCG554350.045.35204748-49
TGCCGTC555150.045.3178550-51
CGTATGC556600.045.23429546-47
Produced by FastQC (version 0.11.2)