FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n01_34298-1.341000000357c1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n01_34298-1.341000000357c1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5401618
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT1221682.261692700224266TruSeq Adapter, Index 2 (97% over 37bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175190.3243287474234572No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC134540.24907351834209676No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG121960.22578420021556506No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA92810.17181888834049355No Hit
TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA84600.1566197387523516No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA60390.11179983479024248No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA185200.067.367474
TCGGAAG186350.067.15573
GATCGGA188500.066.498781
GAAGAGC189250.065.873856
ATCGGAA192750.064.950552
GGAAGAG203600.061.5575455
CGAGTGG1350.045.844131
GCCGTCT145250.043.1015950-51
CGTCTTC146050.042.8841452-53
ATGCCGT145450.042.81273348-49
CTGCTTG146950.042.76775458-59
TGCCGTC145650.042.57556550-51
TCGTATG147050.042.55449344-45
TATGCCG145250.042.49561348-49
TCTCGTA146600.042.36113442-43
CGTATGC145950.042.33826446-47
CGAGGCG2700.042.317661
CGCGTTG459.636317E-442.215036
CTCGTAT146200.042.08718544-45
ATCTCGT148000.041.71972742-43