##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n01_34298-1.341000000357c1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5401618 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.67115242136708 33.0 33.0 33.0 33.0 33.0 2 32.702171830736646 33.0 33.0 33.0 33.0 33.0 3 32.73210712049612 33.0 33.0 33.0 33.0 33.0 4 36.66071554856341 37.0 37.0 37.0 37.0 37.0 5 36.653275555583534 37.0 37.0 37.0 37.0 37.0 6 36.517820401220526 37.0 37.0 37.0 37.0 37.0 7 36.444367780172534 37.0 37.0 37.0 37.0 37.0 8 36.36645186682953 37.0 37.0 37.0 37.0 37.0 9 36.38564556027472 37.0 37.0 37.0 37.0 37.0 10-11 36.33390023137512 37.0 37.0 37.0 37.0 37.0 12-13 36.314993674117645 37.0 37.0 37.0 37.0 37.0 14-15 38.10489134922166 40.0 37.0 40.0 37.0 40.0 16-17 38.07791480256471 40.0 37.0 40.0 37.0 40.0 18-19 38.071813297423105 40.0 37.0 40.0 37.0 40.0 20-21 38.01578221562502 40.0 37.0 40.0 33.0 40.0 22-23 37.8984864349904 40.0 37.0 40.0 33.0 40.0 24-25 37.854446945341195 40.0 37.0 40.0 33.0 40.0 26-27 37.73593088219123 40.0 37.0 40.0 33.0 40.0 28-29 37.59866265996596 40.0 37.0 40.0 33.0 40.0 30-31 37.54310939796187 40.0 37.0 40.0 33.0 40.0 32-33 37.415812077048024 40.0 37.0 40.0 33.0 40.0 34-35 37.315007281151686 40.0 37.0 40.0 33.0 40.0 36-37 37.227210902362955 40.0 37.0 40.0 33.0 40.0 38-39 37.11731031331723 40.0 37.0 40.0 33.0 40.0 40-41 37.01020666400326 40.0 37.0 40.0 33.0 40.0 42-43 36.83891993102807 37.0 37.0 40.0 33.0 40.0 44-45 36.718607091430755 37.0 37.0 40.0 33.0 40.0 46-47 36.66233460048452 37.0 37.0 40.0 33.0 40.0 48-49 36.406372405453325 37.0 37.0 40.0 33.0 40.0 50-51 36.22673632233897 37.0 37.0 40.0 33.0 40.0 52-53 36.08967822234004 37.0 37.0 40.0 33.0 40.0 54-55 35.899874630157115 37.0 37.0 40.0 30.0 40.0 56-57 35.72209271370171 37.0 37.0 40.0 27.0 40.0 58-59 35.40153653960721 37.0 33.0 40.0 27.0 40.0 60-61 35.13588409991229 37.0 33.0 40.0 27.0 40.0 62-63 34.85640515489989 37.0 33.0 37.0 27.0 40.0 64-65 34.57463115681264 37.0 33.0 37.0 27.0 40.0 66-67 34.168654373559924 37.0 33.0 37.0 27.0 40.0 68-69 33.886069229627125 37.0 33.0 37.0 27.0 40.0 70-71 33.494588751000165 37.0 33.0 37.0 27.0 37.0 72-73 33.02896650596173 37.0 33.0 37.0 24.5 37.0 74-75 32.568216412193536 37.0 33.0 37.0 22.0 37.0 76-77 30.02973858943746 33.0 30.0 35.0 22.0 37.0 78-79 32.30111079680199 33.0 33.0 37.0 22.0 37.0 80-81 32.695523637547126 37.0 33.0 37.0 24.5 37.0 82-83 32.62479520025296 37.0 33.0 37.0 22.0 37.0 84-85 32.44354367524693 35.0 33.0 37.0 22.0 37.0 86-87 32.34128487057026 33.0 33.0 37.0 22.0 37.0 88-89 32.213977274957244 33.0 33.0 37.0 22.0 37.0 90-91 32.054378706528304 33.0 33.0 37.0 22.0 37.0 92-93 31.88120568688863 33.0 33.0 37.0 22.0 37.0 94-95 31.749258092667787 33.0 33.0 37.0 22.0 37.0 96-97 31.571560780492067 33.0 33.0 37.0 15.0 37.0 98-99 31.397369362291077 33.0 33.0 37.0 15.0 37.0 100-101 29.72270253098238 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.0722320004660304 2306 2 0.07693524537086915 2306 3 0.09799392009558261 2306 4 0.17096995891585465 2306 5 0.1727318293221174 2306 6 0.1927913642330239 2306 7 0.2129940525842855 2306 8 0.2160756762374092 2306 9 0.1845878924746458 2306 10-11 0.18176598783472997 2306 12-13 0.22821436116547744 2306 14-15 0.4609170667559894 2306 16-17 0.48375713604661286 2306 18-19 0.3290744552052516 2306 20-21 0.4743290928117787 2306 22-23 0.4043571522360665 2306 24-25 0.3317917219605988 2306 26-27 0.4768627175526845 2306 28-29 0.4504451523323638 2306 30-31 0.4618017804732162 2306 32-33 0.47681355111651413 2306 34-35 0.5271284018716855 2306 36-37 0.5219086386729543 2306 38-39 0.5157276760976046 2306 40-41 0.5062722418194952 2306 42-43 0.5419741144148702 2306 44-45 0.5713690171045371 2306 46-47 0.516372850112333 2306 48-49 0.6180287606171859 2306 50-51 0.6220004131239492 2306 52-53 0.5634857210811361 2306 54-55 0.5261008339407951 2306 56-57 0.5871164179072039 2306 58-59 0.5206884588418745 2306 60-61 0.5307325439181412 2306 62-63 0.5502584798741594 2306 64-65 0.5297526197205826 2306 66-67 0.5549149688989417 2306 68-69 0.6031731681110131 2306 70-71 0.6244832806139513 2306 72-73 0.38122277257379267 2306 74-75 0.3939645390761868 2306 76-77 0.401167594687994 2306 78-79 0.46677797876920835 2306 80-81 0.577997714501933 2306 82-83 0.5696417568212624 2306 84-85 0.5248080287383772 2306 86-87 0.6096106221021529 2306 88-89 0.41938612833352806 2306 90-91 0.5501867862425982 2306 92-93 0.6514900551911325 2306 94-95 0.5097955207681935 2306 96-97 0.6186561235166366 2306 98-99 0.6210829340726285 2306 100-101 0.4102364312444422 2307 1 0.1193765688128181 2307 2 0.07925980738598781 2307 3 0.12307167010906284 2307 4 0.15919067716563973 2307 5 0.1784778231516242 2307 6 0.16661523234611053 2307 7 0.21405642469151331 2307 8 0.22252752868786985 2307 9 0.2516903837176372 2307 10-11 0.23106156568423586 2307 12-13 0.19280449638242914 2307 14-15 0.473959210447056 2307 16-17 0.4338102462515607 2307 18-19 0.42582589266975646 2307 20-21 0.4545972530044935 2307 22-23 0.49133073411631045 2307 24-25 0.48561869486596265 2307 26-27 0.42709948876584036 2307 28-29 0.4315802910486468 2307 30-31 0.415725632530318 2307 32-33 0.48214882080082333 2307 34-35 0.40210968995389607 2307 36-37 0.42308319548261863 2307 38-39 0.36658908514799293 2307 40-41 0.333954169962702 2307 42-43 0.4036654466513312 2307 44-45 0.5457299235582838 2307 46-47 0.4934021194439282 2307 48-49 0.47991125822993297 2307 50-51 0.43526160595894225 2307 52-53 0.4466098365527529 2307 54-55 0.3811712414341457 2307 56-57 0.4439063337389939 2307 58-59 0.42265122631169305 2307 60-61 0.5243218254852238 2307 62-63 0.47906154380684285 2307 64-65 0.3456089518419603 2307 66-67 0.5233331158722123 2307 68-69 0.5486549421010665 2307 70-71 0.4017854457305461 2307 72-73 0.34578997466591943 2307 74-75 0.2460738573340322 2307 76-77 0.40849586212485534 2307 78-79 0.4850184654285954 2307 80-81 0.4625605405394282 2307 82-83 0.3908920495056165 2307 84-85 0.557055110393236 2307 86-87 0.3628844446766024 2307 88-89 0.4100799558843846 2307 90-91 0.39793091439658923 2307 92-93 0.39647048699134757 2307 94-95 0.2922170089773459 2307 96-97 0.45885248146760205 2307 98-99 0.48410769171093193 2307 100-101 0.5125492494835484 2201 1 0.1144391711953503 2201 2 0.11062907493755603 2201 3 0.10046912447425171 2201 4 0.16197669461912056 2201 5 0.18690715570161132 2201 6 0.20647340912913137 2201 7 0.13373212418927238 2201 8 0.24558068586274828 2201 9 0.18700265846813835 2201 10-11 0.19135084780746325 2201 12-13 0.2469037507100893 2201 14-15 0.4073013770163598 2201 16-17 0.392766414071815 2201 18-19 0.40599525143453974 2201 20-21 0.45834542062436157 2201 22-23 0.44366295466314654 2201 24-25 0.3881649333875856 2201 26-27 0.4579673324876765 2201 28-29 0.49167740792385217 2201 30-31 0.40833547617621235 2201 32-33 0.4565776882820032 2201 34-35 0.44478973611338546 2201 36-37 0.3431640693713831 2201 38-39 0.5007069454056747 2201 40-41 0.48468343885303256 2201 42-43 0.512142721218865 2201 44-45 0.4639626758309703 2201 46-47 0.5336780214388597 2201 48-49 0.5736398619267433 2201 50-51 0.592885565041783 2201 52-53 0.6213681816173917 2201 54-55 0.5939794124104267 2201 56-57 0.6586160864735291 2201 58-59 0.601561615939481 2201 60-61 0.5802569948288934 2201 62-63 0.6615689616332503 2201 64-65 0.5927457892056154 2201 66-67 0.660344681744931 2201 68-69 0.6345661849198905 2201 70-71 0.46986128361567125 2201 72-73 0.37952952034844145 2201 74-75 0.45100908900814574 2201 76-77 0.39039718121613376 2201 78-79 0.5704794144496503 2201 80-81 0.544481045969988 2201 82-83 0.5098253494124592 2201 84-85 0.6140457931827825 2201 86-87 0.6940098132378125 2201 88-89 0.6166419487047818 2201 90-91 0.6422482124940032 2201 92-93 0.7237702912038486 2201 94-95 0.5900142816574885 2201 96-97 0.4941869346307186 2201 98-99 0.5110711445148404 2201 100-101 0.5404246226917415 1101 1 0.13510497013749756 1101 2 0.09386729611019007 1101 3 0.10724077080584493 1101 4 0.1467212942380769 1101 5 0.13801188586613478 1101 6 0.13892571388914376 1101 7 0.13232202250206626 1101 8 0.20376454594987337 1101 9 0.18447720705794524 1101 10-11 0.16139029512458336 1101 12-13 0.17753479132417738 1101 14-15 0.3414276192875647 1101 16-17 0.2757131451427526 1101 18-19 0.30299392339756537 1101 20-21 0.28638896041928064 1101 22-23 0.3176631518553208 1101 24-25 0.31112418191762714 1101 26-27 0.28470111851422786 1101 28-29 0.314265272926761 1101 30-31 0.3855063123026099 1101 32-33 0.36467145483805297 1101 34-35 0.362959228214244 1101 36-37 0.46747283579716026 1101 38-39 0.46505663858334145 1101 40-41 0.3910662573549004 1101 42-43 0.4330762593636095 1101 44-45 0.29684142100182953 1101 46-47 0.41587250088705474 1101 48-49 0.4623026246226445 1101 50-51 0.4554737340246149 1101 52-53 0.455601438639917 1101 54-55 0.527150832020375 1101 56-57 0.5579722066553501 1101 58-59 0.3829323690043509 1101 60-61 0.3796981701731994 1101 62-63 0.42345354045524886 1101 64-65 0.3596786060888988 1101 66-67 0.3606350565956049 1101 68-69 0.5022618581801623 1101 70-71 0.4447569089497492 1101 72-73 0.4110346710316932 1101 74-75 0.48691156099659594 1101 76-77 0.1955482197615801 1101 78-79 0.47511977658844273 1101 80-81 0.4669074167023268 1101 82-83 0.40267987685790274 1101 84-85 0.5796990717049866 1101 86-87 0.44651091171073176 1101 88-89 0.483230661298478 1101 90-91 0.5323826377469132 1101 92-93 0.5894351712975805 1101 94-95 0.5032485905689938 1101 96-97 0.5462463344795907 1101 98-99 0.6135358690261263 1101 100-101 0.4251737178579873 2304 1 0.08292712224214682 2304 2 0.07394454245674353 2304 3 0.09537172755172918 2304 4 0.10955242641725249 2304 5 0.126800256365307 2304 6 0.11598493405939792 2304 7 0.11416863309106162 2304 8 0.10447030898508558 2304 9 0.07804455780132002 2304 10-11 0.14681194457375568 2304 12-13 0.10307934090400295 2304 14-15 0.2465906134498752 2304 16-17 0.23194143730188443 2304 18-19 0.2631279439552756 2304 20-21 0.21855965782193465 2304 22-23 0.1940574472117973 2304 24-25 0.23733043333315607 2304 26-27 0.3332993628165255 2304 28-29 0.2953899871468124 2304 30-31 0.33614829357005505 2304 32-33 0.21294482848366414 2304 34-35 0.19448028574205267 2304 36-37 0.26688457083092487 2304 38-39 0.2955881404044405 2304 40-41 0.3358877200465642 2304 42-43 0.34675015189485947 2304 44-45 0.3894125441511278 2304 46-47 0.3686623964680962 2304 48-49 0.3734335920656804 2304 50-51 0.43326434889689835 2304 52-53 0.3577411502668397 2304 54-55 0.2881134069280975 2304 56-57 0.2059192802834673 2304 58-59 0.3295548954724268 2304 60-61 0.3309947986891544 2304 62-63 0.29871658895170583 2304 64-65 0.2685740532335288 2304 66-67 0.23025261405221897 2304 68-69 0.35282077975232795 2304 70-71 0.28451064668214343 2304 72-73 0.19309219072367512 2304 74-75 0.1595669804688029 2304 76-77 0.1271526597435475 2304 78-79 0.35283551600876706 2304 80-81 0.3054882025151002 2304 82-83 0.3345127840265434 2304 84-85 0.27993227883651883 2304 86-87 0.26380242883244165 2304 88-89 0.32263835539091446 2304 90-91 0.38196028007251925 2304 92-93 0.42949571441042167 2304 94-95 0.31762243647541766 2304 96-97 0.4348169395547643 2304 98-99 0.4448530351514215 2304 100-101 0.5040029892860431 2202 1 0.09203753478454502 2202 2 0.05635645190666594 2202 3 0.03825179078469887 2202 4 0.04410305698107919 2202 5 0.10362406596212992 2202 6 0.0656982804640478 2202 7 0.12941942613040425 2202 8 0.12446559890076969 2202 9 0.1291216234980439 2202 10-11 0.12959188319794634 2202 12-13 0.13734727165577 2202 14-15 0.1254891385250474 2202 16-17 0.0520708500556708 2202 18-19 0.17906381393489568 2202 20-21 0.19339548536945728 2202 22-23 0.18848396946574297 2202 24-25 0.10159255228190744 2202 26-27 0.1595668949166722 2202 28-29 0.12657009498259697 2202 30-31 0.03163966269371343 2202 32-33 0.22556504201552485 2202 34-35 0.2612462693201536 2202 36-37 0.1661470610390623 2202 38-39 0.25294428342078845 2202 40-41 0.20643146603140394 2202 42-43 0.23416167199618343 2202 44-45 0.1993573470968215 2202 46-47 0.28167468489546366 2202 48-49 0.3171503025455493 2202 50-51 0.18398298561864124 2202 52-53 0.32813340777717315 2202 54-55 0.3035492912929456 2202 56-57 0.3180069045627647 2202 58-59 0.18128750532945048 2202 60-61 0.03464722013591626 2202 62-63 0.13101000119073092 2202 64-65 0.07372741351002077 2202 66-67 0.14101025540600176 2202 68-69 0.21667311361289165 2202 70-71 0.14042169052056153 2202 72-73 0.1052277772368484 2202 74-75 0.14939750960216003 2202 76-77 0.21250415969177894 2202 78-79 0.19593522525495644 2202 80-81 0.22363870797054375 2202 82-83 0.2001649076044103 2202 84-85 0.14245919083528946 2202 86-87 0.10184181230410871 2202 88-89 0.17550095909589913 2202 90-91 0.17769469919456782 2202 92-93 0.3688023198815955 2202 94-95 0.08263294458049941 2202 96-97 0.10221243606639518 2202 98-99 0.2459035484799763 2202 100-101 0.34410434826768466 1102 1 0.04329758125744121 1102 2 0.06742254403670955 1102 3 0.03053993562915025 1102 4 0.06736535137515176 1102 5 0.06345622107415494 1102 6 0.016443171636332465 1102 7 0.09491527506470021 1102 8 0.10356051078859707 1102 9 0.08032560055966087 1102 10-11 0.11190875648139809 1102 12-13 0.10353785877927635 1102 14-15 0.14612901229290998 1102 16-17 0.1919757474916537 1102 18-19 0.14325553659461576 1102 20-21 0.06677465735568688 1102 22-23 0.08686833038458275 1102 24-25 0.05314470230065638 1102 26-27 0.07837907066867444 1102 28-29 0.12349284893844725 1102 30-31 0.16490330918455243 1102 32-33 0.18454656989774065 1102 34-35 0.1548138457823498 1102 36-37 0.11779696074113133 1102 38-39 0.12042832956313987 1102 40-41 0.05601455880887585 1102 42-43 0.16389864094249873 1102 44-45 0.2064526999836218 1102 46-47 0.19100445808244615 1102 48-49 0.19618794324761524 1102 50-51 0.04124756764468884 1102 52-53 0.19799680661405006 1102 54-55 0.150834723905227 1102 56-57 0.1487255343501488 1102 58-59 0.2710451070152544 1102 60-61 0.009025408355185505 1102 62-63 0.17528094128705618 1102 64-65 0.11961801601945155 1102 66-67 0.11814691798422672 1102 68-69 0.17998374265300754 1102 70-71 0.04020479233344787 1102 72-73 0.08141095837488876 1102 74-75 -0.02452407666298484 1102 76-77 -0.060314402587227534 1102 78-79 0.22060082246291302 1102 80-81 0.2517354115071697 1102 82-83 0.11572192243840362 1102 84-85 0.08644271390429736 1102 86-87 0.11316297001405928 1102 88-89 0.1685982920568847 1102 90-91 0.17534746080800545 1102 92-93 0.1029816421579639 1102 94-95 -0.056244797414677805 1102 96-97 0.0353829832186392 1102 98-99 -0.010103294158628984 1102 100-101 0.05687263135719789 2305 1 0.04430348077677593 2305 2 0.06194760069639926 2305 3 0.05801081026579169 2305 4 0.04610176878631478 2305 5 0.04187723597011228 2305 6 -0.005935259544152416 2305 7 -0.0032175442893205286 2305 8 -0.0014709952410214555 2305 9 0.0012944371978775848 2305 10-11 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1213 100-101 0.033872264176878275 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 13.0 8 88.0 9 248.0 10 207.0 11 102.0 12 132.0 13 330.0 14 937.0 15 2531.0 16 5160.0 17 7879.0 18 10306.0 19 12282.0 20 14718.0 21 18003.0 22 22085.0 23 27383.0 24 33979.0 25 41963.0 26 53329.0 27 70541.0 28 99508.0 29 133633.0 30 131137.0 31 140836.0 32 184341.0 33 257350.0 34 389655.0 35 656027.0 36 1198185.0 37 1582314.0 38 306385.0 39 31.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.540022511109015 24.987535048401853 24.25201015893555 14.220432281553578 2 30.79632806318403 23.23046539018494 32.850786560619426 13.122419986011598 3 36.670697557657725 18.075491454597493 28.776970159681785 16.476840828063 4 29.863124552675707 17.67077487447143 33.856224763502595 18.60987580935027 5 31.30543477898659 16.650751682181152 35.70317264197505 16.3406408968572 6 26.841346426200445 17.532506000979705 39.468729554737116 16.15741801808273 7 33.09208463093836 20.133078644213644 29.269248584405638 17.505588140442363 8 29.701637546379622 28.642306805109136 24.52703986101942 17.129015787491824 9 30.392134356779763 25.197635227074556 25.492954148183006 18.917276267962674 10-11 24.905074738717175 27.276243895810477 29.48574852942211 18.332932836050237 12-13 23.938993834810237 26.16175375600422 28.87409846457117 21.025153944614374 14-15 23.18226686892705 26.909520443689278 29.638249132019332 20.26996355536434 16-17 23.122840230464277 26.789084307701877 30.656943900883032 19.431131560950814 18-19 20.9157793090885 27.042721273514715 31.896257750918338 20.14524166647845 20-21 22.346258102664795 27.289656543650437 31.192866655879776 19.17121869780499 22-23 21.028634383253316 29.819204171787046 30.168701303942637 18.983460141017005 24-25 21.524680197674105 26.662298222495558 31.586720867710376 20.22630071211996 26-27 21.236840517045078 26.737905198035104 30.31620340423925 21.709050880680568 28-29 21.933640994235432 28.40122163396227 29.82395274897262 19.841184622829676 30-31 20.166587122599193 27.77656620664401 30.78317459694484 21.27367207381196 32-33 19.853069950522233 29.287557913203045 30.232774698247823 20.626597438026902 34-35 21.501002107146412 28.055982485247938 30.36718812770544 20.075827279900206 36-37 19.886198912992366 28.861926185820618 31.749727581624615 19.5021473195624 38-39 20.432812909020964 29.828358836185746 29.58200672465176 20.156821530141524 40-41 20.486880042239193 27.85306180481478 32.23266621223493 19.427391940711097 42-43 20.329075473311885 29.390351187366452 31.408052179920904 18.87252115940076 44-45 19.995157006659856 28.475116159639573 31.566995296594463 19.962731537106105 46-47 21.654427885297604 28.033392244743165 30.309804653739107 20.002375216220116 48-49 20.8656874198129 30.144285865039443 30.227143552725856 18.762883162421804 50-51 21.545464433074383 29.22667073952454 29.8147729034617 19.41309192393938 52-53 19.922363655045437 28.801195601563652 29.172900315007134 22.10354042838378 54-55 21.69476490764485 28.640978160014125 30.144659044548412 19.519597887792617 56-57 20.536368118828857 28.389790260520442 29.66221199172055 21.41162962893015 58-59 20.353955346616186 28.28059985307793 30.360523790589305 21.004921009716575 60-61 21.204538471955946 28.391183691182032 30.509912992348404 19.894364844513614 62-63 20.168279898257456 28.37634965399279 30.07230244616426 21.383068001585492 64-65 21.616190753737506 28.192263441718268 29.89175454142503 20.29979126311919 66-67 20.900455026918422 30.881402759417142 28.24621095588241 19.97193125778202 68-69 21.446491220598144 30.791477586054285 28.071216650179785 19.690814543167782 70-71 20.692335332323978 31.625863315184006 28.271566087097334 19.410235265394686 72-73 20.26911050661944 31.683828391271398 28.3262795932587 19.720781508850457 74-75 21.319115960103215 30.84595037251629 27.8289619262152 20.005971741165297 76-77 21.70707979856913 29.555288597292122 28.4522681155489 20.28536348858985 78-79 21.47369993790317 29.464630542382658 29.205579013123277 19.856090506590892 80-81 20.496653099853816 30.080973211468994 29.09861043844799 20.3237632502292 82-83 19.552825698382975 29.925503347628517 29.22234862544975 21.299322328538754 84-85 19.410419323730167 29.317994706268628 29.88870834176466 21.382877628236542 86-87 19.6430599920622 29.365765558586933 30.518552135314064 20.47262231403681 88-89 19.7791023187202 29.96887857515422 30.048746653493264 20.203272452632316 90-91 20.203053843746012 29.660124320531477 29.430290300041484 20.706531535681023 92-93 19.555250691914758 30.405864689303563 29.248417720054075 20.7904668987276 94-95 19.429808055067575 30.765738304042813 29.344924557517515 20.459529083372104 96-97 20.160667697986057 31.112685237760658 28.498800718559703 20.22784634569358 98-99 20.44030369117854 30.370775233232184 28.289319568873932 20.89960150671535 100-101 20.183654666199075 30.37715799370974 28.37717058459091 21.06201675550027 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5414.0 1 3256.5 2 1273.0 3 2009.0 4 2130.0 5 1447.5 6 1247.0 7 1381.0 8 1455.5 9 1376.5 10 1300.0 11 1209.5 12 1109.5 13 1099.5 14 1113.0 15 1101.0 16 1151.5 17 1226.0 18 1304.5 19 1450.5 20 1696.0 21 2040.5 22 2475.5 23 3145.0 24 4098.5 25 5176.5 26 6639.0 27 8616.0 28 10985.0 29 13725.5 30 17151.0 31 22391.5 32 34451.0 33 56153.0 34 86287.0 35 129002.0 36 184379.0 37 275439.5 38 385863.5 39 447127.5 40 444722.5 41 389931.5 42 340382.0 43 331891.5 44 323771.5 45 311065.5 46 306350.0 47 273524.5 48 214136.0 49 170924.0 50 141260.5 51 116987.0 52 94283.0 53 67449.5 54 44724.5 55 31056.5 56 21311.0 57 14683.0 58 10403.5 59 7177.5 60 5062.5 61 3866.0 62 2845.0 63 1886.5 64 1290.0 65 920.0 66 649.5 67 496.5 68 400.0 69 269.5 70 170.5 71 130.5 72 112.0 73 93.5 74 59.5 75 38.0 76 24.5 77 17.5 78 15.5 79 14.5 80 10.5 81 6.5 82 5.5 83 4.0 84 1.5 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19442322652212726 2 0.0 3 0.0 4 0.026899347565858968 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 8.330837167678277E-5 48-49 0.0034804386389411467 50-51 1.0182134316051228E-4 52-53 0.002832484637010614 54-55 0.009969235143988339 56-57 0.014347552899890367 58-59 0.014727068815306821 60-61 0.0036377988965528474 62-63 0.0026103289792058604 64-65 0.010367264030888522 66-67 0.017559553452317436 68-69 0.02525169310380705 70-71 0.02115106992016096 72-73 0.017346654280254545 74-75 0.021900845265252003 76-77 0.015865616561556186 78-79 0.006960877277882293 80-81 0.0037396202397133598 82-83 6.109280589630736E-4 84-85 0.0024992511503034833 86-87 0.01626364544845637 88-89 0.015495357131881595 90-91 0.01817048151128051 92-93 0.018975795770822743 94-95 0.018151968539796778 96-97 0.020225421345974482 98-99 0.028269307455654955 100-101 0.016365466791616882 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5401618.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.82201801326196 #Duplication Level Percentage of deduplicated Percentage of total 1 85.94806331519302 29.069369456514377 2 6.1941846453879 4.189996493075605 3 2.1067525804452343 2.137638711759145 4 1.0242190684898176 1.3856462313595599 5 0.8864609192123568 1.4990948588826543 6 0.5985579120569071 1.2146661890141508 7 0.3711153820806111 0.8786309796410317 8 0.27967151449822925 0.7567244000924291 9 0.24546223676477963 0.7471825374090554 >10 1.8100527514249403 12.413806705133048 >50 0.24642085156106364 5.811043406608228 >100 0.2285129112007823 16.603248640794938 >500 0.0421783715700151 9.761485837516423 >1k 0.017851299471080447 9.807244108842195 >5k 2.75692566247833E-4 0.6409422392311471 >10k+ 2.2054807696897277E-4 3.0832792041259873 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT 122168 2.261692700224266 TruSeq Adapter, Index 2 (97% over 37bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17519 0.3243287474234572 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 13454 0.24907351834209676 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 12196 0.22578420021556506 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 9281 0.17181888834049355 No Hit TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA 8460 0.1566197387523516 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 6039 0.11179983479024248 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0013514469183122538 0.0 0.0 2 0.0014625247472146309 0.0 0.0 3 0.0015550896046332783 0.0 0.0 4 0.0016291414905681965 0.0 0.0 5 0.0018698101198566799 0.0 0.0 6 0.002277095492498729 0.0 0.0 7 0.0027028938366245076 0.0 0.0 8 0.0033138218955875813 0.0 0.0 9 0.0037951591541645485 0.0 0.0 10-11 0.006590617848207704 0.0 0.0 12-13 0.009784105429151042 0.0 0.0 14-15 0.0141809361565368 0.0 0.0 16-17 0.020845605890679422 0.0 0.0 18-19 0.029583728430999746 0.0 0.0 20-21 0.035554161734502515 0.0 0.0 22-23 0.04250578252664294 0.0 0.0 24-25 0.04955922466194389 0.0 0.0 26-27 0.05705697811285433 0.0 9.256485741864753E-6 28-29 0.06602651279672128 0.0 1.8512971483729506E-5 30-31 0.09607306551481426 0.0 1.8512971483729506E-5 32-33 0.10656992034608889 0.0 3.702594296745901E-5 34-35 0.11705751869162165 0.0 3.702594296745901E-5 36-37 0.1279986848385058 0.0 4.6282428709323766E-5 38-39 0.13953226607286928 0.0 7.405188593491802E-5 40-41 0.15158421050877718 0.0 7.405188593491802E-5 42-43 0.1645803164903553 0.0 7.405188593491802E-5 44-45 0.1784465321316687 0.0 9.256485741864752E-5 46-47 0.19356237334813384 0.0 1.0182134316051228E-4 48-49 0.20836349404937557 0.0 1.1107782890237703E-4 50-51 0.22415505872499686 0.0 1.1107782890237703E-4 52-53 0.2412332749187373 0.0 1.1107782890237703E-4 54-55 0.2592278832009224 0.0 1.1107782890237703E-4 56-57 0.27789821494226363 0.0 1.1107782890237703E-4 58-59 0.2980680973737869 0.0 1.1107782890237703E-4 60-61 0.3205243317835508 0.0 1.1107782890237703E-4 62-63 0.34348967290911725 0.0 1.2033431464424178E-4 64-65 0.36751025340925625 0.0 1.2959080038610652E-4 66-67 0.39348395240092876 0.0 1.2959080038610652E-4 68-69 0.4214477958271021 0.0 1.388472861279713E-4 70-71 0.451161115058488 0.0 1.4810377186983605E-4 72-73 0.4816797485494161 0.0 1.4810377186983605E-4 74-75 0.5137997540736868 0.0 1.4810377186983605E-4 76-77 0.5474841056883326 0.0 1.6661674335356554E-4 78-79 0.5844452532555986 0.0 1.6661674335356554E-4 80-81 0.6234798536290422 0.0 1.6661674335356554E-4 82-83 0.667161209844902 0.0 1.6661674335356554E-4 84-85 0.7146192122434427 0.0 1.8512971483729504E-4 86-87 0.7645301833635774 0.0 1.8512971483729504E-4 88-89 0.8188564981825815 0.0 1.8512971483729504E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAAGA 18520 0.0 67.36747 4 TCGGAAG 18635 0.0 67.1557 3 GATCGGA 18850 0.0 66.49878 1 GAAGAGC 18925 0.0 65.87385 6 ATCGGAA 19275 0.0 64.95055 2 GGAAGAG 20360 0.0 61.557545 5 CGAGTGG 135 0.0 45.84413 1 GCCGTCT 14525 0.0 43.10159 50-51 CGTCTTC 14605 0.0 42.88414 52-53 ATGCCGT 14545 0.0 42.812733 48-49 CTGCTTG 14695 0.0 42.767754 58-59 TGCCGTC 14565 0.0 42.575565 50-51 TCGTATG 14705 0.0 42.554493 44-45 TATGCCG 14525 0.0 42.495613 48-49 TCTCGTA 14660 0.0 42.361134 42-43 CGTATGC 14595 0.0 42.338264 46-47 CGAGGCG 270 0.0 42.31766 1 CGCGTTG 45 9.636317E-4 42.21503 6 CTCGTAT 14620 0.0 42.087185 44-45 ATCTCGT 14800 0.0 41.719727 42-43 >>END_MODULE