##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n01_34274-2.3410000003583d.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4679685 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.694045646234734 33.0 33.0 33.0 33.0 33.0 2 32.72850587165589 33.0 33.0 33.0 33.0 33.0 3 32.75515894766421 33.0 33.0 33.0 33.0 33.0 4 36.69056571115364 37.0 37.0 37.0 37.0 37.0 5 36.68258803744269 37.0 37.0 37.0 37.0 37.0 6 36.55286178450045 37.0 37.0 37.0 37.0 37.0 7 36.49862138156735 37.0 37.0 37.0 37.0 37.0 8 36.438392755067916 37.0 37.0 37.0 37.0 37.0 9 36.44974437382003 37.0 37.0 37.0 37.0 37.0 10-11 36.412603732943566 37.0 37.0 37.0 37.0 37.0 12-13 36.39484751644609 37.0 37.0 37.0 37.0 37.0 14-15 38.2786386263178 40.0 37.0 40.0 37.0 40.0 16-17 38.25426230611676 40.0 37.0 40.0 37.0 40.0 18-19 38.247248906710595 40.0 37.0 40.0 37.0 40.0 20-21 38.176302571647454 40.0 37.0 40.0 37.0 40.0 22-23 38.07407934508412 40.0 37.0 40.0 37.0 40.0 24-25 38.037997963538146 40.0 37.0 40.0 35.0 40.0 26-27 37.955901732702095 40.0 37.0 40.0 33.0 40.0 28-29 37.82170861927673 40.0 37.0 40.0 33.0 40.0 30-31 37.78470527396609 40.0 37.0 40.0 33.0 40.0 32-33 37.67157949733796 40.0 37.0 40.0 33.0 40.0 34-35 37.56754760202877 40.0 37.0 40.0 33.0 40.0 36-37 37.46907184992152 40.0 37.0 40.0 33.0 40.0 38-39 37.41132234327738 40.0 37.0 40.0 33.0 40.0 40-41 37.330235688940604 40.0 37.0 40.0 33.0 40.0 42-43 37.18446914696182 40.0 37.0 40.0 33.0 40.0 44-45 37.058741133217296 40.0 37.0 40.0 33.0 40.0 46-47 37.00071596699351 40.0 37.0 40.0 33.0 40.0 48-49 36.802916542459585 37.0 37.0 40.0 33.0 40.0 50-51 36.651886077802246 37.0 37.0 40.0 33.0 40.0 52-53 36.51306273819712 37.0 37.0 40.0 33.0 40.0 54-55 36.33760317200837 37.0 37.0 40.0 33.0 40.0 56-57 36.17068467215208 37.0 37.0 40.0 33.0 40.0 58-59 35.94368253418766 37.0 37.0 40.0 30.0 40.0 60-61 35.67785459918777 37.0 37.0 40.0 27.0 40.0 62-63 35.405810113287544 37.0 33.0 40.0 27.0 40.0 64-65 35.16176195620004 37.0 33.0 38.5 27.0 40.0 66-67 34.85127150652234 37.0 33.0 37.0 27.0 40.0 68-69 34.59045523363218 37.0 33.0 37.0 27.0 40.0 70-71 34.26245666107869 37.0 33.0 37.0 27.0 40.0 72-73 33.9448335731999 37.0 33.0 37.0 27.0 38.5 74-75 33.58735929875622 37.0 33.0 37.0 27.0 37.0 76-77 30.986850824361042 33.0 30.0 35.0 24.5 37.0 78-79 33.28858021426656 35.0 33.0 37.0 27.0 37.0 80-81 33.648719839048994 37.0 33.0 37.0 27.0 37.0 82-83 33.55350018217038 37.0 33.0 37.0 27.0 37.0 84-85 33.36267815034559 37.0 33.0 37.0 27.0 37.0 86-87 33.24515442812924 37.0 33.0 37.0 27.0 37.0 88-89 33.09934910148867 37.0 33.0 37.0 27.0 37.0 90-91 32.94615299961429 35.0 33.0 37.0 27.0 37.0 92-93 32.81003721404325 33.0 33.0 37.0 27.0 37.0 94-95 32.67574580340343 33.0 33.0 37.0 27.0 37.0 96-97 32.51028317076897 33.0 33.0 37.0 27.0 37.0 98-99 32.33596663023259 33.0 33.0 37.0 24.5 37.0 100-101 30.64517056169379 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.10920192325531985 2306 2 0.06895312168318668 2306 3 0.11814769985394236 2306 4 0.11656987672590446 2306 5 0.10634416017630599 2306 6 0.17430537651587485 2306 7 0.2095127709262954 2306 8 0.23373743415777426 2306 9 0.1928163571616679 2306 10-11 0.205822050213051 2306 12-13 0.17701154825827103 2306 14-15 0.3897212488899342 2306 16-17 0.34487878679691875 2306 18-19 0.307389236774668 2306 20-21 0.4407872353700242 2306 22-23 0.42834820553878217 2306 24-25 0.34172046731267613 2306 26-27 0.47365457847720904 2306 28-29 0.3807710075809183 2306 30-31 0.4430745408585821 2306 32-33 0.47626780564433346 2306 34-35 0.5397118508126937 2306 36-37 0.4215870468413314 2306 38-39 0.5012883709809159 2306 40-41 0.4663617438322092 2306 42-43 0.5374418880462883 2306 44-45 0.46134667056219314 2306 46-47 0.45574539418890936 2306 48-49 0.5328634753640671 2306 50-51 0.5451675913909995 2306 52-53 0.5603139549342941 2306 54-55 0.46283859195905563 2306 56-57 0.5142892642253045 2306 58-59 0.4614723953484585 2306 60-61 0.46753476702451024 2306 62-63 0.3903836405402217 2306 64-65 0.3656556280748049 2306 66-67 0.5029494794514235 2306 68-69 0.5192668603151134 2306 70-71 0.48476512499317437 2306 72-73 0.37937500368251875 2306 74-75 0.3892317495765738 2306 76-77 0.3377539562075036 2306 78-79 0.3635224399498753 2306 80-81 0.30874860367590173 2306 82-83 0.4070012609268403 2306 84-85 0.43877259100757726 2306 86-87 0.42820448292071944 2306 88-89 0.3870861228081921 2306 90-91 0.5060002726536652 2306 92-93 0.5373775612541607 2306 94-95 0.38928870968256746 2306 96-97 0.5741819851384662 2306 98-99 0.5923798086369274 2306 100-101 0.28539622118017505 2307 1 0.1184416032466018 2307 2 0.10894480569687204 2307 3 0.0892024241973175 2307 4 0.11826050655508169 2307 5 0.1377729647816892 2307 6 0.22034339393692903 2307 7 0.16135108103322438 2307 8 0.18788968117391391 2307 9 0.20275566554946067 2307 10-11 0.16708045841688346 2307 12-13 0.12990811857034146 2307 14-15 0.366859487564426 2307 16-17 0.4204308157437566 2307 18-19 0.4109961096904655 2307 20-21 0.3480282766044098 2307 22-23 0.40189321899886465 2307 24-25 0.4476578836372269 2307 26-27 0.36812340905730423 2307 28-29 0.4343616022082273 2307 30-31 0.4399824498337992 2307 32-33 0.3840681772968182 2307 34-35 0.48981617783944387 2307 36-37 0.4462993306641465 2307 38-39 0.3928783007604082 2307 40-41 0.4049880404740591 2307 42-43 0.42985577452856916 2307 44-45 0.4655114728757752 2307 46-47 0.35355306192410296 2307 48-49 0.3635969036206106 2307 50-51 0.3405551385933805 2307 52-53 0.3475047005190959 2307 54-55 0.3530631637587476 2307 56-57 0.39063143923782206 2307 58-59 0.46313353516959666 2307 60-61 0.45334331299370234 2307 62-63 0.41589524074349526 2307 64-65 0.321056693653631 2307 66-67 0.45017867765533026 2307 68-69 0.48001350421013456 2307 70-71 0.4264843944208465 2307 72-73 0.40909084051244093 2307 74-75 0.3849835718582213 2307 76-77 0.40680623062682386 2307 78-79 0.45285218105405534 2307 80-81 0.4869360498088682 2307 82-83 0.42110963347323604 2307 84-85 0.6189752040182412 2307 86-87 0.6357694635655804 2307 88-89 0.4899869990533219 2307 90-91 0.5521216654794827 2307 92-93 0.6892599692103047 2307 94-95 0.5949677504284523 2307 96-97 0.5916777406837355 2307 98-99 0.5769840159891118 2307 100-101 0.5044283081704073 2201 1 0.12984658365798651 2201 2 0.09615368743378383 2201 3 0.0749673452068862 2201 4 0.13036061460150705 2201 5 0.11615150572793453 2201 6 0.11206817960515991 2201 7 0.11278648916650269 2201 8 0.2111975810945097 2201 9 0.18488123624826613 2201 10-11 0.2032052552200483 2201 12-13 0.1932204975260774 2201 14-15 0.3329437304817304 2201 16-17 0.3390201452391821 2201 18-19 0.3353709043230353 2201 20-21 0.3790784447517126 2201 22-23 0.4269069385054607 2201 24-25 0.42628919826895384 2201 26-27 0.46298465608549577 2201 28-29 0.47933473113023695 2201 30-31 0.4930769180016341 2201 32-33 0.47976494697621774 2201 34-35 0.4907938856887455 2201 36-37 0.4782854059131907 2201 38-39 0.47530619737990776 2201 40-41 0.4467897278816224 2201 42-43 0.4319917748271749 2201 44-45 0.4735770479551533 2201 46-47 0.5118482860141569 2201 48-49 0.4505536919411739 2201 50-51 0.43359371191149165 2201 52-53 0.5544875914820651 2201 54-55 0.5501677861964112 2201 56-57 0.5480377485012369 2201 58-59 0.41260296553103615 2201 60-61 0.43007860121164043 2201 62-63 0.6188937238328549 2201 64-65 0.33488159721429156 2201 66-67 0.542822202580787 2201 68-69 0.5203909228913446 2201 70-71 0.18861724041892813 2201 72-73 0.34015299392763154 2201 74-75 0.41197647172824503 2201 76-77 0.23079693278002367 2201 78-79 0.365961539955002 2201 80-81 0.4613419533275547 2201 82-83 0.4038100763373791 2201 84-85 0.46113271693283764 2201 86-87 0.5741999609613515 2201 88-89 0.4090024365974969 2201 90-91 0.5692346409059255 2201 92-93 0.6387928167658501 2201 94-95 0.4863933966997891 2201 96-97 0.449884860971153 2201 98-99 0.4653704256799429 2201 100-101 0.4566805315539604 1101 1 0.09631228385715218 1101 2 0.09815445473061146 1101 3 0.09051930916026407 1101 4 0.10501051501617553 1101 5 0.15851331067128882 1101 6 0.14539432973413113 1101 7 0.16191797445726053 1101 8 0.11330732086661044 1101 9 0.17058886350525881 1101 10-11 0.15138059887107858 1101 12-13 0.16811944290106595 1101 14-15 0.354509587405353 1101 16-17 0.3180098846847699 1101 18-19 0.2611135497012995 1101 20-21 0.27244818387079306 1101 22-23 0.31054617022910946 1101 24-25 0.26473313662400244 1101 26-27 0.29836916158717486 1101 28-29 0.3545856943326342 1101 30-31 0.36426418740445143 1101 32-33 0.43064684856631885 1101 34-35 0.4976812318212325 1101 36-37 0.4426766701571623 1101 38-39 0.40122940239508864 1101 40-41 0.4465253369847062 1101 42-43 0.3950125163297642 1101 44-45 0.39062662839072004 1101 46-47 0.49462512507772516 1101 48-49 0.5430187146335683 1101 50-51 0.5398073469394049 1101 52-53 0.49191673896631727 1101 54-55 0.503455081719963 1101 56-57 0.4717731372105405 1101 58-59 0.5347541311692012 1101 60-61 0.48665041692979827 1101 62-63 0.53023277924148 1101 64-65 0.3828301393503182 1101 66-67 0.5289224164443809 1101 68-69 0.6079664689781339 1101 70-71 0.4236072410719487 1101 72-73 0.3816284077781589 1101 74-75 0.454485001783425 1101 76-77 0.17075367029920585 1101 78-79 0.554611750553498 1101 80-81 0.6427967970928634 1101 82-83 0.6038563590291233 1101 84-85 0.6450475633102144 1101 86-87 0.6169832077697279 1101 88-89 0.5003442265214275 1101 90-91 0.625472682503208 1101 92-93 0.6575400822505557 1101 94-95 0.41389670260633693 1101 96-97 0.5251124379457224 1101 98-99 0.6693086174714935 1101 100-101 0.7024642762889997 2304 1 0.07887472318289923 2304 2 0.08139004735262745 2304 3 0.07071258424630855 2304 4 0.1090099413686616 2304 5 0.08133609771567762 2304 6 0.15174504899986374 2304 7 0.07801099360826669 2304 8 0.1068652592656889 2304 9 0.10797942920996206 2304 10-11 0.11021157142425153 2304 12-13 0.09772053724402241 2304 14-15 0.17154978685817923 2304 16-17 0.20728543406258382 2304 18-19 0.2356824315299022 2304 20-21 0.21623469874150203 2304 22-23 0.28665430278581283 2304 24-25 0.20437284185742755 2304 26-27 0.18929659517644382 2304 28-29 0.21696577993849786 2304 30-31 0.23992101380988373 2304 32-33 0.24138906019812367 2304 34-35 0.1321902116111886 2304 36-37 0.24660648658994688 2304 38-39 0.17558735844486506 2304 40-41 0.2427408078169364 2304 42-43 0.1955158920247655 2304 44-45 0.28837991583225175 2304 46-47 0.20813617882408408 2304 48-49 0.32424135605975124 2304 50-51 0.28040702922693583 2304 52-53 0.2608866004083765 2304 54-55 0.2534339008886164 2304 56-57 0.13874482991197823 2304 58-59 0.19650651401271801 2304 60-61 0.25744513937265623 2304 62-63 0.21020972302402186 2304 64-65 0.17913584934943572 2304 66-67 0.24132103527767157 2304 68-69 0.2978688385836392 2304 70-71 0.1935146852516283 2304 72-73 0.0998856224988387 2304 74-75 0.14504287399241633 2304 76-77 -0.07096506591118867 2304 78-79 0.2242463812127511 2304 80-81 0.31203362526336065 2304 82-83 0.3276603039589645 2304 84-85 0.29831911921878174 2304 86-87 0.2653070538556932 2304 88-89 0.18529480458160918 2304 90-91 0.16667976925636196 2304 92-93 0.40443863489374365 2304 94-95 0.20762684557694655 2304 96-97 0.3245798282907941 2304 98-99 0.39388764844846236 2304 100-101 0.2121793279172728 2202 1 0.00891139050148837 2202 2 0.011076006936086458 2202 3 0.018894518540491845 2202 4 0.07132402702064411 2202 5 0.10536144020785798 2202 6 0.11776172225227555 2202 7 0.13028760036457498 2202 8 0.10627493672367194 2202 9 0.10851320744718862 2202 10-11 0.14436705894924273 2202 12-13 0.1255510453577955 2202 14-15 0.1186120441334424 2202 16-17 0.08056222974339278 2202 18-19 0.15376569933837203 2202 20-21 0.19321591139731709 2202 22-23 0.14924312723636746 2202 24-25 0.16458377872339014 2202 26-27 0.1960652810734942 2202 28-29 0.20913831856491782 2202 30-31 0.16466480158646846 2202 32-33 0.2281035766511863 2202 34-35 0.2546026444398848 2202 36-37 0.19461902079925864 2202 38-39 0.1447386151254122 2202 40-41 0.20772997126723425 2202 42-43 0.16762767545875334 2202 44-45 0.16178380315342622 2202 46-47 0.22030561083987266 2202 48-49 0.20762438185858656 2202 50-51 0.09566161829619091 2202 52-53 0.13482433248947956 2202 54-55 0.15809494771662003 2202 56-57 0.2744287765181923 2202 58-59 0.2767369619899682 2202 60-61 0.027535397387644878 2202 62-63 0.1604498367512548 2202 64-65 -0.03565673948832426 2202 66-67 0.0677212718324327 2202 68-69 0.1301796180568573 2202 70-71 -0.03973935300957265 2202 72-73 0.09592070258552354 2202 74-75 0.08726626923178316 2202 76-77 -0.00456179592078243 2202 78-79 0.20789483263055075 2202 80-81 0.19389813712636084 2202 82-83 0.23389404747133824 2202 84-85 0.2867951903342245 2202 86-87 0.09313200732304949 2202 88-89 0.24363010033493282 2202 90-91 0.28817089579187183 2202 92-93 0.21682679358499968 2202 94-95 0.0972195669279401 2202 96-97 0.20848206385304024 2202 98-99 0.26090828246312014 2202 100-101 0.2466749128049095 1102 1 0.09119951672522575 1102 2 0.052745378159364975 1102 3 0.047152855410558914 1102 4 0.09027922410852085 1102 5 0.09871952469226386 1102 6 0.09807629487883673 1102 7 0.09557856904759632 1102 8 0.11127711820120112 1102 9 0.10467903778173593 1102 10-11 0.07983271226907362 1102 12-13 0.06582545977671117 1102 14-15 0.14711328585185157 1102 16-17 0.12519731075526863 1102 18-19 0.1429320182763263 1102 20-21 0.07678498696203206 1102 22-23 0.15664219972205018 1102 24-25 0.12246773957328827 1102 26-27 0.08789965997058147 1102 28-29 0.14297511551393427 1102 30-31 0.09622085485969478 1102 32-33 0.18441390163784632 1102 34-35 0.14251868549207813 1102 36-37 0.0842085879869856 1102 38-39 0.14507846792620427 1102 40-41 0.11388536379647007 1102 42-43 0.1182355468478633 1102 44-45 0.24352210193381296 1102 46-47 0.16105373875172546 1102 48-49 0.12197315434652722 1102 50-51 -0.008928162878433454 1102 52-53 0.08241216519453332 1102 54-55 -0.020635289701843362 1102 56-57 -0.0327005833162346 1102 58-59 0.08133001240884852 1102 60-61 -0.013576300206075587 1102 62-63 -0.002867429732575033 1102 64-65 -0.02238711480944744 1102 66-67 -0.019683667460675736 1102 68-69 -0.004211494116262315 1102 70-71 -0.09008381247272723 1102 72-73 -0.017299318741834213 1102 74-75 -0.17328301730024265 1102 76-77 -0.09272436771041015 1102 78-79 0.08044804106686598 1102 80-81 0.13335333837509467 1102 82-83 0.07515574393568158 1102 84-85 0.14478890862997673 1102 86-87 0.04751175441431599 1102 88-89 0.07446495832490285 1102 90-91 0.046992475894889196 1102 92-93 0.029354805064208733 1102 94-95 -0.16231426261722248 1102 96-97 0.08989557807662152 1102 98-99 0.14860480862360959 1102 100-101 0.2312982602019389 2305 1 0.0034715705882533143 2305 2 0.00931969785641229 2305 3 0.03748042401753082 2305 4 0.07084534681358434 2305 5 0.05505822713191577 2305 6 0.011245831944364681 2305 7 0.02917278827310099 2305 8 0.01655786405983406 2305 9 0.04928313937249129 2305 10-11 0.033011957977983286 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82-83 -0.3328265495166036 1213 84-85 -0.21774437405443337 1213 86-87 -0.31586745023460594 1213 88-89 -0.2834430465087294 1213 90-91 -0.2153190049863838 1213 92-93 -0.23146583145926058 1213 94-95 -0.10685854476997036 1213 96-97 -0.22929452523935367 1213 98-99 -0.11762041801076606 1213 100-101 0.0394926371085873 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 3.0 8 30.0 9 141.0 10 214.0 11 186.0 12 173.0 13 284.0 14 570.0 15 1284.0 16 2434.0 17 3943.0 18 5672.0 19 7558.0 20 9775.0 21 12300.0 22 15624.0 23 19336.0 24 23587.0 25 29277.0 26 36047.0 27 44935.0 28 57845.0 29 74201.0 30 87468.0 31 107450.0 32 143173.0 33 202263.0 34 306203.0 35 522859.0 36 995469.0 37 1528662.0 38 440466.0 39 253.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 33.15187660514267 33.2158949670648 15.764960545205206 17.867267882587328 2 30.170705934266945 25.8315036161622 29.969453072161905 14.02833737740895 3 31.57355676717557 23.74025174771379 25.324119037926696 19.362072447183944 4 27.602048461035828 23.597272509056975 29.354940825347942 19.445738204559255 5 29.540706265485817 21.94827215934406 29.953789624729016 18.557231950441107 6 27.469434374322205 22.258848619084404 31.506458233834117 18.765258772759278 7 30.86451331660144 23.332147356072046 25.096966996710247 20.706372330616272 8 27.231512377435664 26.93486847939551 25.28285557681767 20.550763566351154 9 26.15806405773038 24.182290047300192 28.662741188776597 20.996904706192833 10-11 22.39112247939765 29.259415964963452 31.02559253454025 17.32386902109864 12-13 22.00780608096485 29.003212823085317 30.10968686994958 18.879294226000255 14-15 21.762212627559332 29.03769163950138 30.514382912525097 18.685712820414192 16-17 22.05811929649111 28.442181471616145 30.75544614648208 18.744253085410666 18-19 21.228554913418318 28.389261243011017 31.23413221189033 19.14805163168034 20-21 21.772416305798362 28.758207016070525 30.784443824744613 18.6849328533865 22-23 21.315547948205914 29.816974860487406 30.160726630104378 18.706750561202306 24-25 21.178893451161777 28.951168721826363 30.661283825727587 19.208654001284277 26-27 21.031629265645016 28.927534652439213 30.48552413250037 19.555311949415398 28-29 21.080211595438584 29.70385827251193 30.28836342617078 18.927566705878707 30-31 20.468450333729727 29.662049902931503 30.719129599535012 19.15037016380376 32-33 20.382760805481563 30.07484478121925 30.336860280125695 19.205534133173494 34-35 20.905082286521424 30.088585022282484 30.38863726938886 18.617695421807237 36-37 20.75395031930568 29.788372507978632 30.76091660015578 18.696760572559906 38-39 20.771932298861994 29.105719722588162 31.100907432872084 19.021440545677752 40-41 20.6218687796294 29.3819028417511 29.89329409992339 20.102934278696107 42-43 20.94070434227945 29.565312622537625 30.293887302243633 19.20009573293929 44-45 20.50537589602719 29.23385868920664 30.5614159927431 19.69934942202306 46-47 20.979013208484382 29.34653779322375 30.077591819015886 19.596857179275982 48-49 20.54451507488533 30.061075689042138 30.20273547199217 19.191673764080367 50-51 21.146326122050425 29.377165812922485 29.867188582639347 19.60931948238774 52-53 20.54144360457513 29.250096803890273 29.67904702232943 20.529412569205167 54-55 21.05542106055011 29.450067874386228 29.87491323362466 19.619597831439005 56-57 20.55631307410526 29.246391338357174 30.098621078772908 20.098674508764656 58-59 20.684334641586727 29.5455536339723 30.11647544396125 19.653636280479724 60-61 20.797161353714333 29.720991602939982 29.882556127939104 19.599290915406584 62-63 20.312412018190912 29.718354569348442 29.668264705696938 20.300968706763705 64-65 20.6504881487448 29.537513712253507 29.852192966802722 19.959805172198976 66-67 20.593532262440746 29.83518775208413 29.603080402221092 19.96819958325403 68-69 20.47821203830852 30.033336614306815 29.775817600958597 19.712633746426064 70-71 20.5114964498026 30.592227493054402 29.41892735424399 19.47734870289901 72-73 20.409074000437496 30.491648169558367 29.39102258241254 19.708255247591598 74-75 20.556685386354044 30.003024360529423 29.390415021346854 20.04987523176968 76-77 20.64402200918795 29.76755493480916 29.44173431888103 20.146688737121856 78-79 20.363487508876744 29.57068088113703 29.912650503925427 20.153181106060803 80-81 20.352925727396972 29.831992078660345 29.66179268925827 20.15328950468441 82-83 20.041099152854848 29.88540723644685 29.578034380072822 20.49545923062548 84-85 20.09171587801889 29.949479229940508 29.637783291828367 20.32102160021224 86-87 20.129018152347726 29.74301521237439 30.000936106659736 20.127030528618146 88-89 20.342129647093447 30.129644528788447 29.608762495895213 19.919463328222896 90-91 20.577213829587603 29.87637101348668 29.211665375035423 20.33474978189029 92-93 20.20125854718323 30.15151830888533 29.235633224918956 20.41158991901248 94-95 19.762203190792818 30.470743086709962 29.21843176400448 20.548621958492745 96-97 19.959403834386283 30.607358733615076 29.06224011582539 20.370997316173256 98-99 20.146533300287782 30.22356727169312 28.94700645446335 20.682892973555756 100-101 20.142601085954226 30.113766480409552 28.86806407409905 20.875568359537173 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 80.0 1 56.0 2 48.0 3 97.0 4 104.5 5 82.0 6 80.5 7 74.5 8 76.5 9 76.5 10 82.5 11 93.0 12 95.0 13 104.5 14 130.5 15 164.5 16 215.5 17 302.0 18 427.0 19 644.0 20 1002.5 21 1577.0 22 2519.5 23 4023.5 24 6217.0 25 9184.0 26 13721.5 27 19991.0 28 26892.5 29 34335.0 30 42523.0 31 51275.0 32 65521.5 33 89477.5 34 118840.5 35 156305.0 36 204273.0 37 261817.0 38 315320.0 39 330482.5 40 335839.0 41 322471.0 42 286100.0 43 272255.0 44 255788.5 45 240232.0 46 242525.5 47 225162.5 48 178233.0 49 133435.0 50 101767.5 51 81749.0 52 65848.5 53 49825.5 54 38505.0 55 29726.5 56 20324.5 57 13219.0 58 8683.0 59 5888.0 60 4213.0 61 3149.5 62 2113.5 63 1304.5 64 847.0 65 603.0 66 461.5 67 312.5 68 202.5 69 155.5 70 120.5 71 90.5 72 65.0 73 49.5 74 34.0 75 23.5 76 19.0 77 15.0 78 11.5 79 6.5 80 4.5 81 3.5 82 2.5 83 2.0 84 0.5 85 1.0 86 1.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19552598091538212 2 0.0 3 0.0 4 0.026625723739952582 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 7.479135882009151E-5 48-49 0.003493824904881418 50-51 9.616031848297482E-5 52-53 0.0025856441192088782 54-55 0.009680138727286132 56-57 0.01421035817581739 58-59 0.014381309853120456 60-61 0.00278864923600627 62-63 0.002040735647805354 64-65 0.010171624799532448 66-67 0.017426386605081324 68-69 0.025546591276976977 70-71 0.021401013102377617 72-73 0.01710585221013808 74-75 0.021582649259512126 76-77 0.015599340553904804 78-79 0.00688080501144842 80-81 0.003707514501510251 82-83 5.662774310664073E-4 84-85 0.0026604354780289698 86-87 0.015620709513567687 88-89 0.01492621832452398 90-91 0.01809950883446215 92-93 0.01875126210418009 94-95 0.01744775556474421 96-97 0.019616704970526862 98-99 0.027662118283602422 100-101 0.016208355904296978 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4679685.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.65023597102502 #Duplication Level Percentage of deduplicated Percentage of total 1 90.65878969089408 45.01230301000422 2 4.336666700317636 4.306330499969141 3 1.4432371872266945 2.1497120072389135 4 0.6869466584144932 1.3642825475914682 5 0.447227411552473 1.1102473258145502 6 0.33201489129496087 0.9890770619213417 7 0.24325098660144023 0.8454228219462305 8 0.19062422865929154 0.7571630347782764 9 0.17210192752394085 0.7690411181368718 >10 1.1944924714494316 12.184917055478241 >50 0.1536863784488352 5.252618720457778 >100 0.11336534578457512 10.670291300396512 >500 0.01655160556453527 5.833283336052548 >1k 0.011001353389369964 8.146684784989517 >5k 0.0 0.0 >10k+ 4.3162878361981054E-5 0.6086253752245266 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT 28400 0.6068784544258855 TruSeq Adapter, Index 7 (97% over 38bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 7.906515075266818E-4 0.0 0.0 2 0.001025710063818398 0.0 0.0 3 0.0015599340553904804 0.0 0.0 4 0.002350585562917162 0.0 0.0 5 0.0028848095544892443 0.0 0.0 6 0.00506444344010334 0.0 0.0 7 0.006303843100550571 0.0 0.0 8 0.008120204671895651 0.0 2.136895966288329E-5 9 0.009210021614702699 0.0 2.136895966288329E-5 10-11 0.01317396363216755 0.0 2.136895966288329E-5 12-13 0.019242748176426405 0.0 2.136895966288329E-5 14-15 0.02808949747686009 0.0 2.136895966288329E-5 16-17 0.039853109771277345 0.0 2.136895966288329E-5 18-19 0.05465111433782402 0.0 2.136895966288329E-5 20-21 0.07022908593206595 0.0 2.136895966288329E-5 22-23 0.0891512997135491 0.0 2.136895966288329E-5 24-25 0.10947318035295112 0.0 2.136895966288329E-5 26-27 0.13035065394358808 0.0 2.136895966288329E-5 28-29 0.1520080945619203 0.0 2.136895966288329E-5 30-31 0.1761763879406413 0.0 3.205343949432494E-5 32-33 0.19991730212610465 0.0 4.273791932576658E-5 34-35 0.2263827586685856 0.0 5.342239915720823E-5 36-37 0.25548728172943264 0.0 6.410687898864988E-5 38-39 0.28789330905819516 0.0 8.547583865153318E-5 40-41 0.322628552990212 0.0 1.175292781458581E-4 42-43 0.36045161159351535 0.0 1.4958271764018306E-4 44-45 0.40214245189580067 0.0 1.4958271764018306E-4 46-47 0.44801092381217966 0.0 1.4958271764018306E-4 48-49 0.4973411671939457 0.0 1.4958271764018306E-4 50-51 0.5487869375823373 0.0 1.6026719747162468E-4 52-53 0.6029786192874094 0.0 1.7095167730306633E-4 54-55 0.6581105352176482 0.0 1.7095167730306633E-4 56-57 0.7183068945879905 0.0 1.7095167730306633E-4 58-59 0.7830227889270325 0.0 1.7095167730306633E-4 60-61 0.8482194848584894 0.0 1.7095167730306633E-4 62-63 0.9187049983065099 0.0 2.1368959662883293E-4 64-65 0.9922355885064913 0.0 2.350585562917162E-4 66-67 1.067347481721526 0.0 2.564275159545995E-4 68-69 1.1462630497565542 0.0 2.564275159545995E-4 70-71 1.2302537457115168 0.0 2.564275159545995E-4 72-73 1.3168728237050145 0.0 2.777964756174828E-4 74-75 1.4087379812957495 0.0 2.777964756174828E-4 76-77 1.504043541392209 0.0 2.777964756174828E-4 78-79 1.6050652981984899 0.0 2.777964756174828E-4 80-81 1.7112583432431885 0.0 2.777964756174828E-4 82-83 1.8243428777791668 0.0 2.777964756174828E-4 84-85 1.9416370973687331 0.0 2.777964756174828E-4 86-87 2.064626144708458 0.0 2.777964756174828E-4 88-89 2.195468284724292 0.0 2.777964756174828E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTGG 25 5.228538E-5 76.14168 1 CGGTGCG 20 0.0020592075 71.38283 1 CGATCGC 35 2.7632958E-4 54.386913 1 CGACGGG 135 0.0 52.876167 1 CGAGCGG 75 2.3501343E-9 50.761124 1 CGCTCGG 55 4.9113823E-5 43.262318 1 CGCTCCG 45 9.540263E-4 42.300934 1 CGAGCGC 45 9.540263E-4 42.300934 1 GTGCGAC 45 9.540263E-4 42.300934 1 CGAGGCG 155 0.0 39.91298 1 CGTGCGG 60 8.204134E-5 39.657124 1 AGCGCCG 120 8.54925E-11 39.657124 1 CGACGTG 75 6.838867E-6 38.070843 1 CGACGCC 55 0.002556971 34.60985 1 CGGAGCG 55 0.002556971 34.60985 1 CCGCCGA 115 1.0372605E-7 33.044582 4 CGCGGGG 190 0.0 32.56059 1 GTGTTTG 58460 0.0 31.967196 1 AACGCCG 75 3.0464216E-4 31.725702 1 GTCCGGC 75 3.0464216E-4 31.725702 1 >>END_MODULE