FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n01_34274-2.3410000003583d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n01_34274-2.3410000003583d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4679685
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT284000.6068784544258855TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGG255.228538E-576.141681
CGGTGCG200.002059207571.382831
CGATCGC352.7632958E-454.3869131
CGACGGG1350.052.8761671
CGAGCGG752.3501343E-950.7611241
CGCTCGG554.9113823E-543.2623181
CGCTCCG459.540263E-442.3009341
CGAGCGC459.540263E-442.3009341
GTGCGAC459.540263E-442.3009341
CGAGGCG1550.039.912981
CGTGCGG608.204134E-539.6571241
AGCGCCG1208.54925E-1139.6571241
CGACGTG756.838867E-638.0708431
CGACGCC550.00255697134.609851
CGGAGCG550.00255697134.609851
CCGCCGA1151.0372605E-733.0445824
CGCGGGG1900.032.560591
GTGTTTG584600.031.9671961
AACGCCG753.0464216E-431.7257021
GTCCGGC753.0464216E-431.7257021
Produced by FastQC (version 0.11.2)