##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n01_34271-1.341000000358dd.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5261404 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.67197348844529 33.0 33.0 33.0 33.0 33.0 2 32.69468738762505 33.0 33.0 33.0 33.0 33.0 3 32.73414263569192 33.0 33.0 33.0 33.0 33.0 4 36.65378233642579 37.0 37.0 37.0 37.0 37.0 5 36.65481495053412 37.0 37.0 37.0 37.0 37.0 6 36.52652200819401 37.0 37.0 37.0 37.0 37.0 7 36.40281320347192 37.0 37.0 37.0 37.0 37.0 8 36.34000905461736 37.0 37.0 37.0 37.0 37.0 9 36.406889301790926 37.0 37.0 37.0 37.0 37.0 10-11 36.33441682106145 37.0 37.0 37.0 37.0 37.0 12-13 36.31669940190869 37.0 37.0 37.0 35.0 37.0 14-15 38.141191115527334 40.0 37.0 40.0 37.0 40.0 16-17 38.15713096732355 40.0 37.0 40.0 37.0 40.0 18-19 38.095269247524044 40.0 37.0 40.0 37.0 40.0 20-21 38.086496399060024 40.0 37.0 40.0 37.0 40.0 22-23 37.88013361072444 40.0 37.0 40.0 33.0 40.0 24-25 37.91725488101655 40.0 37.0 40.0 33.0 40.0 26-27 37.84411100155016 40.0 37.0 40.0 33.0 40.0 28-29 37.63389049766944 40.0 37.0 40.0 33.0 40.0 30-31 37.622999773444505 40.0 37.0 40.0 33.0 40.0 32-33 37.47353510583867 40.0 37.0 40.0 33.0 40.0 34-35 37.28210958519817 40.0 37.0 40.0 33.0 40.0 36-37 36.94895678035749 37.0 37.0 40.0 33.0 40.0 38-39 37.12947865246615 38.5 37.0 40.0 33.0 40.0 40-41 36.895869619592034 38.5 37.0 40.0 33.0 40.0 42-43 36.680896296881976 37.0 37.0 40.0 33.0 40.0 44-45 36.68453230354483 37.0 37.0 40.0 33.0 40.0 46-47 36.68254737708794 37.0 37.0 40.0 33.0 40.0 48-49 36.34966902370546 37.0 37.0 40.0 33.0 40.0 50-51 36.26626200915193 37.0 37.0 40.0 33.0 40.0 52-53 36.173387464638715 37.0 37.0 40.0 33.0 40.0 54-55 35.85756396961723 37.0 37.0 40.0 30.0 40.0 56-57 35.73078146061393 37.0 37.0 40.0 27.0 40.0 58-59 35.436589073943 37.0 33.0 40.0 27.0 40.0 60-61 35.1468952013569 37.0 33.0 40.0 27.0 40.0 62-63 34.90426110597095 37.0 33.0 37.0 27.0 40.0 64-65 34.64867599979017 37.0 33.0 37.0 27.0 40.0 66-67 34.02927184454948 37.0 33.0 37.0 27.0 40.0 68-69 33.853489106709915 37.0 33.0 37.0 27.0 40.0 70-71 33.38567006069103 37.0 33.0 37.0 27.0 38.5 72-73 32.56120286524281 37.0 33.0 37.0 22.0 37.0 74-75 31.822988217593632 35.0 33.0 37.0 18.5 37.0 76-77 29.343223690862743 33.0 27.0 35.0 15.0 37.0 78-79 31.430978689338435 33.0 33.0 37.0 15.0 37.0 80-81 31.714827924257477 35.0 33.0 37.0 15.0 37.0 82-83 31.59183641856812 35.0 33.0 37.0 15.0 37.0 84-85 31.398106284938393 33.0 33.0 37.0 15.0 37.0 86-87 31.278765325757156 33.0 33.0 37.0 15.0 37.0 88-89 31.14410801755577 33.0 33.0 37.0 15.0 37.0 90-91 31.002600066446142 33.0 33.0 37.0 15.0 37.0 92-93 30.876176492054213 33.0 33.0 37.0 15.0 37.0 94-95 30.747145533777676 33.0 33.0 37.0 15.0 37.0 96-97 30.574381001725015 33.0 27.0 37.0 15.0 37.0 98-99 30.39159205413612 33.0 27.0 37.0 15.0 37.0 100-101 28.743561509437406 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.11084309054918862 2306 2 0.11040495988947185 2306 3 0.10882973008870067 2306 4 0.1652531132202455 2306 5 0.14916212331171153 2306 6 0.21879152196179774 2306 7 0.2631856792449909 2306 8 0.25783704570932287 2306 9 0.22190721375237388 2306 10-11 0.22414115639026022 2306 12-13 0.2024362005239695 2306 14-15 0.42168346854695926 2306 16-17 0.4912806304150408 2306 18-19 0.3988152334432158 2306 20-21 0.47332931591864025 2306 22-23 0.46971426871350275 2306 24-25 0.47598016128466014 2306 26-27 0.4728347033090188 2306 28-29 0.4128735458713635 2306 30-31 0.5037202661310332 2306 32-33 0.506090783280797 2306 34-35 0.62433361561407 2306 36-37 0.5587210150750721 2306 38-39 0.5254415092112126 2306 40-41 0.6648647014140465 2306 42-43 0.6157156500662708 2306 44-45 0.6114033501672722 2306 46-47 0.6250860807552243 2306 48-49 0.6034563079807072 2306 50-51 0.6250142662139595 2306 52-53 0.5765671693048589 2306 54-55 0.5888450665779033 2306 56-57 0.7499451480659829 2306 58-59 0.6612991942726367 2306 60-61 0.600960959227173 2306 62-63 0.6729412924365619 2306 64-65 0.6585948194743523 2306 66-67 0.7054020725652279 2306 68-69 0.7434928702656762 2306 70-71 0.6750275570258069 2306 72-73 0.39123627086641477 2306 74-75 0.5060321287321052 2306 76-77 0.34853513564052463 2306 78-79 0.48786452816391446 2306 80-81 0.5902771593899843 2306 82-83 0.44710185511187106 2306 84-85 0.46667880433291486 2306 86-87 0.6164804247657116 2306 88-89 0.5811882361923892 2306 90-91 0.609684072094737 2306 92-93 0.7924666719341111 2306 94-95 0.6843915531122207 2306 96-97 0.7525105887207353 2306 98-99 0.8912371717757885 2306 100-101 0.5961583461591857 2307 1 0.0949299212608139 2307 2 0.10210070472828647 2307 3 0.10744266958860038 2307 4 0.17931385053525162 2307 5 0.15298106664684497 2307 6 0.15016643210164204 2307 7 0.20559188516372018 2307 8 0.1903020477826729 2307 9 0.18117181817756745 2307 10-11 0.21394282122501806 2307 12-13 0.20753989506645354 2307 14-15 0.48701926937521733 2307 16-17 0.47386541584619835 2307 18-19 0.3469118279018488 2307 20-21 0.4331860466930877 2307 22-23 0.4798573749684891 2307 24-25 0.528568043156362 2307 26-27 0.47095963653635664 2307 28-29 0.47356378049464354 2307 30-31 0.4826988753403185 2307 32-33 0.5069572433973732 2307 34-35 0.47348897544400614 2307 36-37 0.49003979091269656 2307 38-39 0.4692248163183521 2307 40-41 0.44926371127738207 2307 42-43 0.5314997104595349 2307 44-45 0.48967521040372475 2307 46-47 0.4905701847883819 2307 48-49 0.45037716042535436 2307 50-51 0.4113218423528977 2307 52-53 0.40995693873774286 2307 54-55 0.3757692146493312 2307 56-57 0.4604324843484804 2307 58-59 0.46089882994290576 2307 60-61 0.524933384609291 2307 62-63 0.3450499684456645 2307 64-65 0.42067139028414857 2307 66-67 0.5111158202180022 2307 68-69 0.5705113963961992 2307 70-71 0.3913664416191125 2307 72-73 0.12267256658191883 2307 74-75 0.1641869036788215 2307 76-77 0.21350588242593105 2307 78-79 0.31260508809452503 2307 80-81 0.3693941124924933 2307 82-83 0.42360874423937744 2307 84-85 0.49856127721189836 2307 86-87 0.37797211236202344 2307 88-89 0.27229384654427946 2307 90-91 0.4169034865958601 2307 92-93 0.4831829637383187 2307 94-95 0.2861476513798422 2307 96-97 0.388307614327033 2307 98-99 0.47603708920403776 2307 100-101 0.35265498624957914 2201 1 0.07062351673124567 2201 2 0.05232158650945706 2201 3 0.058177034668254635 2201 4 0.13985876570480826 2201 5 0.12673952739029914 2201 6 0.14881969218762947 2201 7 0.1538151067089899 2201 8 0.1523232795399636 2201 9 0.1940319457558033 2201 10-11 0.2575059510876372 2201 12-13 0.24390526698663706 2201 14-15 0.43399016452493555 2201 16-17 0.41076330263882 2201 18-19 0.37071639078720864 2201 20-21 0.36274701162485457 2201 22-23 0.4704164399584556 2201 24-25 0.3901084001567696 2201 26-27 0.4128488156097774 2201 28-29 0.4289187509093608 2201 30-31 0.4203719593473281 2201 32-33 0.4478797781029016 2201 34-35 0.4653823943424058 2201 36-37 0.4943541998983605 2201 38-39 0.47668419885453517 2201 40-41 0.5262636075445926 2201 42-43 0.4289758295857595 2201 44-45 0.49566583896135086 2201 46-47 0.47938657452262134 2201 48-49 0.5268365683290313 2201 50-51 0.5151591459766465 2201 52-53 0.5447830755437977 2201 54-55 0.5394721027204454 2201 56-57 0.6139078069592614 2201 58-59 0.5383226491605839 2201 60-61 0.5584859125275372 2201 62-63 0.6314027459419123 2201 64-65 0.5853173714481343 2201 66-67 0.742577393886215 2201 68-69 0.7290734073499507 2201 70-71 0.5538680657936439 2201 72-73 0.36548802831851646 2201 74-75 0.377579409168856 2201 76-77 0.3664347023147094 2201 78-79 0.5338138181323622 2201 80-81 0.6310495223841244 2201 82-83 0.6308585353148608 2201 84-85 0.6301533267301949 2201 86-87 0.6568610412143059 2201 88-89 0.4966343602276595 2201 90-91 0.5832942569732538 2201 92-93 0.5198638810601857 2201 94-95 0.6291248398113609 2201 96-97 0.5280634111265137 2201 98-99 0.5755965761518702 2201 100-101 0.7732881719552225 1101 1 0.06825538319495195 1101 2 0.058187995494243694 1101 3 0.06543571036169737 1101 4 0.12407276546548474 1101 5 0.13198817674160068 1101 6 0.07466249400776803 1101 7 0.14251736865203668 1101 8 0.10096181840107477 1101 9 0.1528196204951371 1101 10-11 0.1782758606773882 1101 12-13 0.18618891403645677 1101 14-15 0.32081595685770736 1101 16-17 0.29435049943307234 1101 18-19 0.3392846828400593 1101 20-21 0.3603636044156602 1101 22-23 0.33816912337246663 1101 24-25 0.32055429141239244 1101 26-27 0.2606338363561491 1101 28-29 0.24695285449364235 1101 30-31 0.3569339676573051 1101 32-33 0.43284001765562863 1101 34-35 0.3503275744931713 1101 36-37 0.312522525003871 1101 38-39 0.34919839485444015 1101 40-41 0.4429103122166964 1101 42-43 0.5347775232492822 1101 44-45 0.44956678996176436 1101 46-47 0.5097732720545665 1101 48-49 0.44045219063725227 1101 50-51 0.4132870991385573 1101 52-53 0.32364133443079623 1101 54-55 0.5080214400353427 1101 56-57 0.46885771865215986 1101 58-59 0.4676807856933678 1101 60-61 0.3751458800220817 1101 62-63 0.4997656620355002 1101 64-65 0.37402934700762813 1101 66-67 0.5169801905286846 1101 68-69 0.43499421917972825 1101 70-71 0.3877116295393108 1101 72-73 0.2067436637818716 1101 74-75 0.1858084191041094 1101 76-77 0.17178058672465113 1101 78-79 0.4398047734808266 1101 80-81 0.4399061813813603 1101 82-83 0.35579441061224415 1101 84-85 0.5116201476426454 1101 86-87 0.5640613637854841 1101 88-89 0.5687625223861588 1101 90-91 0.48127142382120525 1101 92-93 0.6421594108077535 1101 94-95 0.4436350378449667 1101 96-97 0.5007585616914128 1101 98-99 0.5013747120978884 1101 100-101 0.5880227776038218 2304 1 0.07854277588668879 2304 2 0.09416965604470562 2304 3 0.08427949375656141 2304 4 0.14102428904455877 2304 5 0.12163466834072523 2304 6 0.09972206113733506 2304 7 0.08947604099216022 2304 8 0.1478714179686662 2304 9 0.12023435214346989 2304 10-11 0.21585717367690904 2304 12-13 0.1670520193441618 2304 14-15 0.2620706626886573 2304 16-17 0.29570859269633587 2304 18-19 0.3137844283046576 2304 20-21 0.28478193483476133 2304 22-23 0.30037190126027014 2304 24-25 0.3217192280345387 2304 26-27 0.3505536700509495 2304 28-29 0.3936373330951852 2304 30-31 0.3662619014738695 2304 32-33 0.31872780599343997 2304 34-35 0.29566308601661007 2304 36-37 0.31915264056828363 2304 38-39 0.32165273596050525 2304 40-41 0.3989373436646986 2304 42-43 0.28344533202451316 2304 44-45 0.37995279383054026 2304 46-47 0.3808943032096721 2304 48-49 0.4043536685551601 2304 50-51 0.38872052742251384 2304 52-53 0.25368524493713807 2304 54-55 0.33833418749681243 2304 56-57 0.2444218464050465 2304 58-59 0.3149357844088527 2304 60-61 0.404974798569647 2304 62-63 0.31612852399506153 2304 64-65 0.23728202925441622 2304 66-67 0.28368854234367546 2304 68-69 0.2703473823880742 2304 70-71 0.36492872057105785 2304 72-73 0.16583167114813335 2304 74-75 0.1415202922861134 2304 76-77 0.030412854274771917 2304 78-79 0.24768328163225917 2304 80-81 0.23432364201605083 2304 82-83 0.3166689783278791 2304 84-85 0.30613841961586274 2304 86-87 0.13863963173330518 2304 88-89 0.16342749362834397 2304 90-91 0.11779393808470928 2304 92-93 0.2893570924442841 2304 94-95 0.0767648638004026 2304 96-97 0.2537589147452657 2304 98-99 0.3552432218608885 2304 100-101 0.3568713735369613 2202 1 0.07075927950950955 2202 2 0.08659882555254228 2202 3 0.08621889785699466 2202 4 0.09554520194006244 2202 5 0.08539904579228619 2202 6 0.08137503392135415 2202 7 0.14529333436681213 2202 8 0.09351202987630103 2202 9 0.1296505374050838 2202 10-11 0.15702181570433993 2202 12-13 0.10043733767161456 2202 14-15 0.08620722794332636 2202 16-17 0.02027218692231969 2202 18-19 0.11060421065781156 2202 20-21 0.10173990125380072 2202 22-23 0.183864612108934 2202 24-25 0.09202281889988484 2202 26-27 0.10007718782224373 2202 28-29 0.15856103030098012 2202 30-31 0.17464337531222185 2202 32-33 0.2020048503802201 2202 34-35 0.22750990544265193 2202 36-37 0.16476705368921074 2202 38-39 0.29132384273078316 2202 40-41 0.3048101852184857 2202 42-43 0.33043239653766676 2202 44-45 0.21674218221546226 2202 46-47 0.26144616743057725 2202 48-49 0.3384495609904832 2202 50-51 0.3154992787624593 2202 52-53 0.3508577544770972 2202 54-55 0.4343389100297159 2202 56-57 0.38922373144244915 2202 58-59 0.3286612668828539 2202 60-61 0.23209328084785597 2202 62-63 0.29382650205634775 2202 64-65 0.17107927052405358 2202 66-67 0.18250885617401025 2202 68-69 0.2762832266791335 2202 70-71 0.07266001628766361 2202 72-73 0.13633829792212993 2202 74-75 0.17213766285060572 2202 76-77 0.14867130821254193 2202 78-79 0.20003008905598207 2202 80-81 0.24061632053349769 2202 82-83 0.20954594642499913 2202 84-85 0.27349786321889624 2202 86-87 0.21535317678734245 2202 88-89 0.2731901423725631 2202 90-91 0.26670051849979615 2202 92-93 0.27861240177750446 2202 94-95 0.06172587880553593 2202 96-97 0.19053971397892155 2202 98-99 0.31122954148083437 2202 100-101 0.34110137300435994 1102 1 0.03982761873294294 1102 2 0.028387200882171726 1102 3 0.05003242761156912 1102 4 0.120350337563778 1102 5 0.08991498576119739 1102 6 0.15014810502098186 1102 7 0.09569512415450276 1102 8 0.12686122900608865 1102 9 0.10969162183548065 1102 10-11 0.10936890625055185 1102 12-13 0.056546793837789266 1102 14-15 0.06252905621446558 1102 16-17 0.08669145524749666 1102 18-19 0.14019679063908086 1102 20-21 0.05094288532869484 1102 22-23 0.08383067078289486 1102 24-25 0.07140596512402198 1102 26-27 0.04613956137801978 1102 28-29 0.08686217494565085 1102 30-31 0.035710388870562326 1102 32-33 0.08372981700262017 1102 34-35 0.09620440938625308 1102 36-37 0.030335848610000937 1102 38-39 0.11780286649311478 1102 40-41 0.06061739883620021 1102 42-43 0.11997908865020435 1102 44-45 0.12104649908716425 1102 46-47 0.06608674246577095 1102 48-49 0.004084543026529275 1102 50-51 0.0471193755583883 1102 52-53 0.13439370300409337 1102 54-55 0.07040466941521828 1102 56-57 0.10081591131198309 1102 58-59 0.02328507795321144 1102 60-61 -0.18316776539973745 1102 62-63 -0.031750762682676736 1102 64-65 0.03819738406628659 1102 66-67 0.15863453967636332 1102 68-69 0.10089329253938217 1102 70-71 0.07069417680990853 1102 72-73 0.002616137500567106 1102 74-75 -0.038199251677909274 1102 76-77 -0.12431182451722833 1102 78-79 0.035980739844969634 1102 80-81 0.0510299144038342 1102 82-83 0.03032377132044317 1102 84-85 0.012976926135898736 1102 86-87 0.0026253805412252973 1102 88-89 -0.047922048502229586 1102 90-91 -0.12003947896913303 1102 92-93 -0.012773904088252408 1102 94-95 -0.25998899238085826 1102 96-97 -0.018054774267863394 1102 98-99 0.13538904465009338 1102 100-101 -0.04740838472265452 2305 1 -0.018501229461300284 2305 2 -0.01665357371066989 2305 3 0.021431846709418778 2305 4 0.045857316568437057 2305 5 0.06982853528123911 2305 6 0.038685796599132516 2305 7 -0.023653335107546525 2305 8 0.02210673311748934 2305 9 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0.08314281344317109 1213 98-99 0.2682396009378074 1213 100-101 0.2945752940615485 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 25.0 9 56.0 10 61.0 11 48.0 12 77.0 13 253.0 14 707.0 15 1790.0 16 3653.0 17 5958.0 18 8073.0 19 10594.0 20 13909.0 21 18061.0 22 23515.0 23 29931.0 24 38384.0 25 50073.0 26 66730.0 27 98067.0 28 162374.0 29 206345.0 30 145955.0 31 131960.0 32 170902.0 33 239972.0 34 361071.0 35 599891.0 36 1070996.0 37 1443881.0 38 357542.0 39 550.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.21929393591083 24.25993676242186 20.300923122391602 15.219846179275715 2 29.518014583179696 28.35680362123874 28.57168922971891 13.55349256586265 3 34.012157211269084 18.749311020404438 30.837016127254245 16.40151564107223 4 28.65980604429563 18.63415427978389 28.992539448642002 23.713500227278477 5 36.93244236709441 17.283352504388564 28.986008297405025 16.798196831112 6 33.002673811020784 17.83088696477214 32.30430508662707 16.862134137580007 7 30.818808059597778 25.258619182256297 25.44398795454597 18.47858480359995 8 28.17213808329488 30.748883758023528 23.063197579961546 18.015780578720054 9 33.995640707309306 22.54421063275126 24.44393169579831 19.01621696414113 10-11 26.43941617104484 29.39528118350159 27.838985563549194 16.326317081904374 12-13 22.694341662415585 28.170655589268566 27.511677491407237 21.62332525690861 14-15 22.230653643020002 28.474072699986543 27.681394547919147 21.613879109074308 16-17 25.608611313634157 24.828401316454695 28.09719610963157 21.465791260279577 18-19 21.158591889161144 24.80698117840789 31.96843466116649 22.065992271264477 20-21 24.830244930820747 25.123275080187724 31.47945871482213 18.567021274169406 22-23 21.09393234201365 32.57563950610901 27.4632588563813 18.86716929549603 24-25 20.9272182862217 24.803826127018567 31.544232299971643 22.72472328678809 26-27 20.86507707828557 25.05007218605528 27.747384538423585 26.337466197235564 28-29 24.378930034644743 29.295421906396086 27.235724532843324 19.089923526115843 30-31 20.466951406886828 26.045110772713898 30.85201212452037 22.635925695878896 32-33 20.22727203613332 29.937959525632323 27.20282456925946 22.631943868974897 34-35 24.112261670078937 29.994151751129543 27.366088975490193 18.527497603301324 36-37 20.209044962143185 29.979364823533793 31.4020079051143 18.409582309208723 38-39 20.358814871467768 26.05312194235607 31.15956311281171 22.42850007336445 40-41 24.060649970996334 25.905442729735256 30.89126020355023 19.142647095718175 42-43 20.44714680720203 29.551750825445072 30.75668585799532 19.244416509357578 44-45 20.15623586403933 25.885105952707683 31.013546954387078 22.94511122886591 46-47 23.939612281975847 25.850814260308237 27.3528286477309 22.85674480998502 48-49 20.216563200791906 29.837791902681417 30.72470335152266 19.22094154500402 50-51 23.919457915989447 26.184511589456683 30.25219435478989 19.64383613976398 52-53 20.248158903199347 25.972094929243266 26.80564905861061 26.974097108946783 54-55 24.076504818973323 26.03577109608228 30.56125032040477 19.326473764539628 56-57 20.471911541226227 25.97562629610582 30.3693198855934 23.183142277074552 58-59 20.379572999622006 26.20546091570577 30.266413555904286 23.148552528767937 60-61 23.897934433966252 26.277313993426084 30.175944080161248 19.64880749244642 62-63 20.158116815235775 26.237849961036247 30.3214414688385 23.282591754889477 64-65 24.16726193893134 26.281017043097965 30.066473442366682 19.48524757560401 66-67 20.6611910376955 33.39351113824826 26.31971694063821 19.625580883418035 68-69 20.77920263234139 33.4170039854695 26.16099327238148 19.64280010980763 70-71 20.62610016123579 33.64001369881633 26.07616074228346 19.657725397664414 72-73 20.36543369838304 33.60391512886615 26.100459907311386 19.93019126543943 74-75 20.673473123866152 33.011555046585755 25.99319924589838 20.321772583649715 76-77 20.678161800082613 32.27479815526851 26.213981553065302 20.83305849158357 78-79 20.60194028971293 31.754871157388138 26.667151358944093 20.97603719395484 80-81 20.47729446653745 31.42980688405298 26.823515659635817 21.269382989773757 82-83 20.14878062664471 31.375589307006607 26.929922536342954 21.54570753000573 84-85 20.29343717406231 31.15065062805743 26.928907272731596 21.62700492514867 86-87 20.156066589678883 31.040941341963567 27.419407574267073 21.383584494090474 88-89 20.230956536020734 31.0851419602944 27.446262039995677 21.237639463689188 90-91 20.491149156375897 30.676363032199284 27.17318013504145 21.65930767638337 92-93 20.250261954875327 30.960965682010354 27.006369039290952 21.78240332382337 94-95 20.24929067858851 30.880391173020055 26.90311569150794 21.967202456883495 96-97 20.46121431088224 31.121510455196677 26.703326920925498 21.713948312995587 98-99 20.63261797720233 30.784562832374913 26.78775106500183 21.795068125420926 100-101 20.670430779396405 30.855291396844216 26.345161482869305 22.12911634089007 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2543.0 1 1531.5 2 596.0 3 849.5 4 903.0 5 690.0 6 607.0 7 604.5 8 604.0 9 588.5 10 561.0 11 569.0 12 556.5 13 561.0 14 584.0 15 596.5 16 627.5 17 687.5 18 725.0 19 774.0 20 947.0 21 1271.5 22 1701.5 23 2379.0 24 3429.0 25 5192.5 26 8561.0 27 13443.0 28 18537.5 29 23707.5 30 29430.5 31 34651.0 32 45156.0 33 66381.0 34 98015.5 35 133857.5 36 180017.5 37 239977.0 38 288136.0 39 305858.5 40 313652.5 41 316709.5 42 313308.5 43 312357.5 44 300005.0 45 278308.5 46 257315.5 47 228144.0 48 193800.0 49 169480.0 50 150731.0 51 136355.5 52 125454.5 53 110168.5 54 91992.5 55 76155.0 56 63300.5 57 53925.0 58 47072.0 59 40046.0 60 32976.0 61 27053.5 62 22192.0 63 18055.0 64 14749.5 65 11659.5 66 9028.0 67 7200.0 68 6270.5 69 5208.0 70 3692.5 71 2608.5 72 1847.0 73 1342.5 74 1063.0 75 855.0 76 664.5 77 485.0 78 351.0 79 224.5 80 127.5 81 88.5 82 69.0 83 60.0 84 44.5 85 22.0 86 11.0 87 11.0 88 9.5 89 4.5 90 5.0 91 3.0 92 1.0 93 0.0 94 1.0 95 1.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1918879447387047 2 0.0 3 0.0 4 0.025411468117635522 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 5.701900101189721E-5 48-49 0.0032025672235015596 50-51 1.0453483518847822E-4 52-53 0.0026228740465472715 54-55 0.00913254332873887 56-57 0.013180892400583571 58-59 0.013865120412726337 60-61 0.0030125038867952355 62-63 0.0019766587017457696 64-65 0.009617204837339997 66-67 0.01676358629749778 68-69 0.02463220843713959 70-71 0.020821438536177798 72-73 0.016868121132686258 74-75 0.021001998706048804 76-77 0.015224073270176554 78-79 0.006348115445991223 80-81 0.003544681229572943 82-83 6.367121779661854E-4 84-85 0.0026893962143944845 86-87 0.01575625061295426 88-89 0.01445431675651594 90-91 0.017960985318747617 92-93 0.018170054989124575 94-95 0.01745731747647586 96-97 0.019567020513916056 98-99 0.027302598317863445 100-101 0.016079358285355012 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5261404.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.300050892411946 #Duplication Level Percentage of deduplicated Percentage of total 1 90.38457612461994 47.27117931206705 2 5.04200818717312 5.2739456957822375 3 1.3791484336079594 2.1638859979765948 4 0.6535783367525113 1.367287210973372 5 0.4772686575292398 1.2480587539066188 6 0.30379204662611076 0.9533003699553346 7 0.27260383922925646 0.9980036265609898 8 0.18626749936177697 0.7793439756978596 9 0.11203338187597274 0.5273416416386156 >10 0.9459634221154409 9.756050377951292 >50 0.12006624867523712 4.425027185332005 >100 0.10647738747419011 11.846640570069706 >500 0.01361899298046178 4.671019179328104 >1k 0.0025243097251001984 1.9589696948715867 >5k 3.6566359761746105E-5 0.12277752299063012 >10k+ 3.6565893887604466E-5 6.637168884898172 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT 347060 6.596338163729682 TruSeq Adapter, Index 7 (97% over 35bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6420 0.12202066216546001 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0027749247159123305 0.0 0.0 2 0.003117038721983714 0.0 0.0 3 0.0036302097310907887 0.0 0.0 4 0.003934311069820907 0.0 0.0 5 0.004390463077916085 0.0 0.0 6 0.005093697423729483 0.0 0.0 7 0.006424140780673751 0.0 0.0 8 0.007298432129522842 0.0 0.0 9 0.008191729812042565 0.0 0.0 10-11 0.011631876206427031 0.0 0.0 12-13 0.015537677775741988 0.0 0.0 14-15 0.020004166188340604 0.0 1.90063336706324E-5 16-17 0.025344945949788308 0.0 1.90063336706324E-5 18-19 0.032339276740581034 0.0 1.90063336706324E-5 20-21 0.03775608183671127 0.0 1.90063336706324E-5 22-23 0.043553013606254146 0.0 1.90063336706324E-5 24-25 0.04954951187933867 0.0 1.90063336706324E-5 26-27 0.055859614657988624 0.0 1.90063336706324E-5 28-29 0.06285394544878135 0.0 1.90063336706324E-5 30-31 0.08941529675349014 0.0 1.90063336706324E-5 32-33 0.09833877041185204 0.0 1.90063336706324E-5 34-35 0.1063499400540236 0.0 1.90063336706324E-5 36-37 0.11495980920682008 0.0 2.8509500505948604E-5 38-39 0.12513770088744375 0.0 3.80126673412648E-5 40-41 0.13512552923136106 0.0 3.80126673412648E-5 42-43 0.14638678193121074 0.0 3.80126673412648E-5 44-45 0.15825623730852068 0.0 3.80126673412648E-5 46-47 0.1711140220367035 0.0 3.80126673412648E-5 48-49 0.18537827545651314 0.0 3.80126673412648E-5 50-51 0.2003647695558068 0.0 3.80126673412648E-5 52-53 0.2171568653538105 0.0 3.80126673412648E-5 54-55 0.23562151851482988 0.0 3.80126673412648E-5 56-57 0.25393412100648416 0.0 3.80126673412648E-5 58-59 0.2752307178844278 0.0 3.80126673412648E-5 60-61 0.2981048404570339 0.0 3.80126673412648E-5 62-63 0.3212925675352054 0.0 3.80126673412648E-5 64-65 0.3460673234748748 0.0 3.80126673412648E-5 66-67 0.374263219475258 0.0 3.80126673412648E-5 68-69 0.4039701190024564 0.0 3.80126673412648E-5 70-71 0.43604330707164857 0.0 3.80126673412648E-5 72-73 0.4713855845321895 0.0 3.80126673412648E-5 74-75 0.5100729767187617 0.0 3.80126673412648E-5 76-77 0.5525331261389546 0.0 3.80126673412648E-5 78-79 0.5999349223135118 0.0 3.80126673412648E-5 80-81 0.6511474883890307 0.0 3.80126673412648E-5 82-83 0.7077198405596681 0.0 3.80126673412648E-5 84-85 0.770801862012497 0.0 3.80126673412648E-5 86-87 0.8423226956150868 0.0 3.80126673412648E-5 88-89 0.9204862428355625 0.0 3.80126673412648E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 46105 0.0 81.09141 3 GATCGGA 46315 0.0 81.06734 1 ATCGGAA 46460 0.0 80.55359 2 CGGAAGA 46365 0.0 80.411255 4 GAAGAGC 47900 0.0 77.824356 6 GGAAGAG 52315 0.0 71.47443 5 GAGCACA 54215 0.0 68.671715 9 AGAGCAC 54635 0.0 68.152504 8 AAGAGCA 56120 0.0 66.2814 7 ATGCCGT 40595 0.0 45.315063 48-49 GCCGTCT 40925 0.0 45.207573 50-51 TCGTATG 41075 0.0 45.09045 44-45 TATGCCG 40715 0.0 45.012352 48-49 CGTATGC 40805 0.0 44.99909 46-47 TGCCGTC 40930 0.0 44.935135 50-51 GTATGCC 41035 0.0 44.9147 46-47 CGTATCT 40620 0.0 44.863026 38-39 TCTCGTA 40970 0.0 44.81765 42-43 TATCTCG 40780 0.0 44.809296 40-41 TCGTATC 40575 0.0 44.690384 38-39 >>END_MODULE