FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n01_34271-1.341000000358dd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n01_34271-1.341000000358dd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5261404
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT3470606.596338163729682TruSeq Adapter, Index 7 (97% over 35bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64200.12202066216546001No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG461050.081.091413
GATCGGA463150.081.067341
ATCGGAA464600.080.553592
CGGAAGA463650.080.4112554
GAAGAGC479000.077.8243566
GGAAGAG523150.071.474435
GAGCACA542150.068.6717159
AGAGCAC546350.068.1525048
AAGAGCA561200.066.28147
ATGCCGT405950.045.31506348-49
GCCGTCT409250.045.20757350-51
TCGTATG410750.045.0904544-45
TATGCCG407150.045.01235248-49
CGTATGC408050.044.9990946-47
TGCCGTC409300.044.93513550-51
GTATGCC410350.044.914746-47
CGTATCT406200.044.86302638-39
TCTCGTA409700.044.8176542-43
TATCTCG407800.044.80929640-41
TCGTATC405750.044.69038438-39
Produced by FastQC (version 0.11.2)