##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n01_34211-2.34100000035863.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3569610 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.6804267132824 33.0 33.0 33.0 33.0 33.0 2 32.70876230176406 33.0 33.0 33.0 33.0 33.0 3 32.73866836993397 33.0 33.0 33.0 33.0 33.0 4 36.656312314230405 37.0 37.0 37.0 37.0 37.0 5 36.64987883830447 37.0 37.0 37.0 37.0 37.0 6 36.51996520628304 37.0 37.0 37.0 37.0 37.0 7 36.44078652850031 37.0 37.0 37.0 37.0 37.0 8 36.39534402917966 37.0 37.0 37.0 37.0 37.0 9 36.39224901319752 37.0 37.0 37.0 37.0 37.0 10-11 36.35221438756615 37.0 37.0 37.0 37.0 37.0 12-13 36.326108454425 37.0 37.0 37.0 37.0 37.0 14-15 38.14792694440009 40.0 37.0 40.0 37.0 40.0 16-17 38.12849554993403 40.0 37.0 40.0 37.0 40.0 18-19 38.11472541818294 40.0 37.0 40.0 37.0 40.0 20-21 38.05297679578442 40.0 37.0 40.0 33.0 40.0 22-23 37.9209994929418 40.0 37.0 40.0 33.0 40.0 24-25 37.89888349147386 40.0 37.0 40.0 33.0 40.0 26-27 37.82548303596191 40.0 37.0 40.0 33.0 40.0 28-29 37.64137931034483 40.0 37.0 40.0 33.0 40.0 30-31 37.58722731614938 40.0 37.0 40.0 33.0 40.0 32-33 37.444468163188695 40.0 37.0 40.0 33.0 40.0 34-35 37.330613568429044 40.0 37.0 40.0 33.0 40.0 36-37 37.27810475093918 40.0 37.0 40.0 33.0 40.0 38-39 37.149175960399035 40.0 37.0 40.0 33.0 40.0 40-41 37.02701499603598 40.0 37.0 40.0 33.0 40.0 42-43 36.87399617885427 38.5 37.0 40.0 33.0 40.0 44-45 36.72855900224394 37.0 37.0 40.0 33.0 40.0 46-47 36.70688170416376 37.0 37.0 40.0 33.0 40.0 48-49 36.48997215382073 37.0 37.0 40.0 33.0 40.0 50-51 36.36761705060216 37.0 37.0 40.0 33.0 40.0 52-53 36.239796924594 37.0 37.0 40.0 33.0 40.0 54-55 36.01058911197582 37.0 37.0 40.0 30.0 40.0 56-57 35.85109998010987 37.0 37.0 40.0 27.0 40.0 58-59 35.6433835909245 37.0 37.0 40.0 27.0 40.0 60-61 35.40647857889237 37.0 33.0 40.0 27.0 40.0 62-63 35.17964721636257 37.0 33.0 40.0 27.0 40.0 64-65 34.95645154512678 37.0 33.0 40.0 27.0 40.0 66-67 34.57120077543485 37.0 33.0 40.0 27.0 40.0 68-69 34.33676241942397 37.0 33.0 37.0 27.0 40.0 70-71 33.996763511980305 37.0 33.0 37.0 27.0 40.0 72-73 33.55229268743644 37.0 33.0 37.0 24.5 40.0 74-75 33.12828180109312 37.0 33.0 37.0 22.0 40.0 76-77 30.568013032236014 33.0 30.0 35.0 22.0 37.0 78-79 32.71948210028546 35.0 33.0 37.0 22.0 37.0 80-81 32.98710839559504 37.0 33.0 37.0 22.0 37.0 82-83 32.81638246194962 37.0 33.0 37.0 22.0 37.0 84-85 32.58611024733794 37.0 33.0 37.0 22.0 37.0 86-87 32.43022459036141 37.0 33.0 37.0 22.0 37.0 88-89 32.2357914450038 35.0 33.0 37.0 22.0 37.0 90-91 32.04562613282683 33.0 33.0 37.0 22.0 37.0 92-93 31.890033505060778 33.0 33.0 37.0 22.0 37.0 94-95 31.735440426265054 33.0 33.0 37.0 22.0 37.0 96-97 31.548908984454883 33.0 33.0 37.0 15.0 37.0 98-99 31.359555525673674 33.0 33.0 37.0 15.0 37.0 100-101 29.587552001479153 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.10321279574451836 2306 2 0.09900802627393546 2306 3 0.10992940724693767 2306 4 0.12595948724245432 2306 5 0.12122319816269567 2306 6 0.21491520203172598 2306 7 0.2642582650847629 2306 8 0.2448712023526909 2306 9 0.2174389775219865 2306 10-11 0.23820079874090538 2306 12-13 0.21658402347869554 2306 14-15 0.4055721454501722 2306 16-17 0.4341266833869284 2306 18-19 0.3938558885895631 2306 20-21 0.3912357076916777 2306 22-23 0.4404968261133675 2306 24-25 0.40883278397938483 2306 26-27 0.3618463131236851 2306 28-29 0.3971506556953841 2306 30-31 0.40725616473594073 2306 32-33 0.5430810128990018 2306 34-35 0.7193253588256923 2306 36-37 0.5695635923629325 2306 38-39 0.5826782477482126 2306 40-41 0.580999944747397 2306 42-43 0.4697018974071625 2306 44-45 0.4964150311299562 2306 46-47 0.5119547648213185 2306 48-49 0.47866221690472344 2306 50-51 0.4263220696866554 2306 52-53 0.48765248840588526 2306 54-55 0.5011375057015286 2306 56-57 0.6000587727055589 2306 58-59 0.5901419075830105 2306 60-61 0.6532591356893178 2306 62-63 0.5924876064822158 2306 64-65 0.5674549560423756 2306 66-67 0.48864375306239083 2306 68-69 0.6113324796666788 2306 70-71 0.5846968820152512 2306 72-73 0.3099078387313057 2306 74-75 0.27885124521873195 2306 76-77 0.485487151526371 2306 78-79 0.42611077546921194 2306 80-81 0.4662797817491722 2306 82-83 0.4419187800395932 2306 84-85 0.46304752077475086 2306 86-87 0.5701591656133473 2306 88-89 0.529876214019346 2306 90-91 0.45239643107921523 2306 92-93 0.464698352181351 2306 94-95 0.38092819229321506 2306 96-97 0.4753921687465201 2306 98-99 0.6329739355413224 2306 100-101 0.3334292601083497 2307 1 0.09729540991438768 2307 2 0.06609877168989442 2307 3 0.088858164950679 2307 4 0.17565979742167315 2307 5 0.1289894472659796 2307 6 0.17835358968650183 2307 7 0.26283170674374645 2307 8 0.18876365991155808 2307 9 0.23724995683620875 2307 10-11 0.21611950401811697 2307 12-13 0.18681152576334625 2307 14-15 0.45123173424524055 2307 16-17 0.4611548812807982 2307 18-19 0.4351865107115884 2307 20-21 0.394889429927602 2307 22-23 0.44701893009248295 2307 24-25 0.4443212369413416 2307 26-27 0.43958180355513576 2307 28-29 0.40249862916044776 2307 30-31 0.38640306783879197 2307 32-33 0.513367017038135 2307 34-35 0.4716691000312281 2307 36-37 0.4746288125499163 2307 38-39 0.610267244760351 2307 40-41 0.47178516925978187 2307 42-43 0.5073825495988018 2307 44-45 0.45230053941897097 2307 46-47 0.43265157819290323 2307 48-49 0.5690069036912533 2307 50-51 0.4607759096767481 2307 52-53 0.48264367219346127 2307 54-55 0.37966471554511827 2307 56-57 0.34523433114242863 2307 58-59 0.42870100861247096 2307 60-61 0.5079265432657607 2307 62-63 0.579700943131229 2307 64-65 0.3908276237712798 2307 66-67 0.5288374266070335 2307 68-69 0.5592630149398801 2307 70-71 0.5009260639683006 2307 72-73 0.2794182242602261 2307 74-75 0.2865476502712454 2307 76-77 0.3308778364619869 2307 78-79 0.3149424132721208 2307 80-81 0.3387688497481136 2307 82-83 0.38484152245643344 2307 84-85 0.5079719988034697 2307 86-87 0.48735917134413853 2307 88-89 0.461355533187394 2307 90-91 0.46603486778685266 2307 92-93 0.5907996112163048 2307 94-95 0.28578882654882776 2307 96-97 0.5238470546065024 2307 98-99 0.6136680677200346 2307 100-101 0.5899740484800269 2201 1 0.08210523313033491 2201 2 0.09348306233651016 2201 3 0.09991133345302217 2201 4 0.15936968651191563 2201 5 0.15477365896597206 2201 6 0.15598100005773574 2201 7 0.23979684975761018 2201 8 0.23110006994188126 2201 9 0.1947991639096216 2201 10-11 0.24169000873825297 2201 12-13 0.21906189550524857 2201 14-15 0.43771116326632864 2201 16-17 0.4806424266815199 2201 18-19 0.4581446318331359 2201 20-21 0.481023115017706 2201 22-23 0.5273932601355185 2201 24-25 0.5029865263140465 2201 26-27 0.5045743574528672 2201 28-29 0.544170817391759 2201 30-31 0.5517803904203475 2201 32-33 0.6305716784775797 2201 34-35 0.658201491216893 2201 36-37 0.6037494804178749 2201 38-39 0.5986466473804555 2201 40-41 0.5163160007369285 2201 42-43 0.45201161579862514 2201 44-45 0.5629084797066142 2201 46-47 0.6338062366846628 2201 48-49 0.6178838046260537 2201 50-51 0.6297638228969831 2201 52-53 0.65050201426304 2201 54-55 0.6698808767910407 2201 56-57 0.6939619896316245 2201 58-59 0.7114866070048365 2201 60-61 0.7440367109760828 2201 62-63 0.7888457435474905 2201 64-65 0.6608813164484744 2201 66-67 0.6090145502841011 2201 68-69 0.7588430663064614 2201 70-71 0.391418858807036 2201 72-73 0.3834433808078046 2201 74-75 0.4061498669284731 2201 76-77 0.39923603499224924 2201 78-79 0.5637678258835876 2201 80-81 0.48388726327977594 2201 82-83 0.5288278016352876 2201 84-85 0.48201797496056287 2201 86-87 0.5762691247707536 2201 88-89 0.581694350064538 2201 90-91 0.47035146041969966 2201 92-93 0.4806903739483701 2201 94-95 0.45937656624026957 2201 96-97 0.5612408098770878 2201 98-99 0.5499263564750123 2201 100-101 0.3329840359972316 1101 1 0.06125204881882951 1101 2 0.09257323396602146 1101 3 0.09799686024628329 1101 4 0.1408450658363094 1101 5 0.15569870479393444 1101 6 0.14260456230064733 1101 7 0.17006285091414242 1101 8 0.08743439514128681 1101 9 0.11292602365850968 1101 10-11 0.1303450430243558 1101 12-13 0.21572737013774912 1101 14-15 0.29682530824421605 1101 16-17 0.24181657643534749 1101 18-19 0.2143327231191563 1101 20-21 0.2771222075890165 1101 22-23 0.31356164481411497 1101 24-25 0.38733209370280264 1101 26-27 0.3440682248175122 1101 28-29 0.2949063766099087 1101 30-31 0.3316486013858153 1101 32-33 0.3016794040295636 1101 34-35 0.3452868155009057 1101 36-37 0.3877599125702531 1101 38-39 0.28013092306459697 1101 40-41 0.4681746961091804 1101 42-43 0.449883512900513 1101 44-45 0.45769029648374016 1101 46-47 0.5076175191417462 1101 48-49 0.42151670921727913 1101 50-51 0.44396408112552166 1101 52-53 0.4058012011825838 1101 54-55 0.4374293853631528 1101 56-57 0.4758763633396015 1101 58-59 0.49418980103209975 1101 60-61 0.4261678749242819 1101 62-63 0.3950732539012165 1101 64-65 0.35663239747324127 1101 66-67 0.5056047570308237 1101 68-69 0.47544294199153825 1101 70-71 0.30839899534274906 1101 72-73 0.3613984061975515 1101 74-75 0.42938575390724054 1101 76-77 0.33788612360467596 1101 78-79 0.4618295900317264 1101 80-81 0.6426368038296104 1101 82-83 0.5397553993138047 1101 84-85 0.5132087878303224 1101 86-87 0.597478602437377 1101 88-89 0.5325621548409458 1101 90-91 0.47475969446887945 1101 92-93 0.6076711308881215 1101 94-95 0.4649250853475415 1101 96-97 0.6323041414233437 1101 98-99 0.7188290139113178 1101 100-101 0.3292523935185194 2304 1 0.06971549776545771 2304 2 0.060035936086663355 2304 3 0.05929216913698809 2304 4 0.12013019705189976 2304 5 0.12200816864234554 2304 6 0.1394157890057528 2304 7 0.09667938182057867 2304 8 0.09353578979540345 2304 9 0.1268482495473151 2304 10-11 0.1224998965248787 2304 12-13 0.10097842524655931 2304 14-15 0.1478595753995009 2304 16-17 0.22770243363526532 2304 18-19 0.23114325510240974 2304 20-21 0.20210006333002184 2304 22-23 0.2587168283075201 2304 24-25 0.23337217915358366 2304 26-27 0.21425857860460695 2304 28-29 0.28314820928614637 2304 30-31 0.21940499716884432 2304 32-33 0.11286442582646572 2304 34-35 0.11054985456164701 2304 36-37 0.2445534238681688 2304 38-39 0.13557233196051044 2304 40-41 0.2307830292977755 2304 42-43 0.26633886833758424 2304 44-45 0.3281932751036791 2304 46-47 0.16409753720451192 2304 48-49 0.31672052324024236 2304 50-51 0.22769543218584687 2304 52-53 0.2647535606316751 2304 54-55 0.11590342881800098 2304 56-57 0.18387000663467035 2304 58-59 0.18868152515805292 2304 60-61 0.10373964837548755 2304 62-63 0.24937184535266255 2304 64-65 0.10860627593164907 2304 66-67 0.1444822602004905 2304 68-69 0.17188857132268964 2304 70-71 0.1196239636460632 2304 72-73 0.05880796209660133 2304 74-75 0.1264436068693655 2304 76-77 0.06823765286114991 2304 78-79 0.2979169165683686 2304 80-81 0.16058146923420225 2304 82-83 0.22168828918302808 2304 84-85 0.20209931703469408 2304 86-87 0.17039313428168867 2304 88-89 0.13254912880624659 2304 90-91 0.15447468482961568 2304 92-93 0.37468400061040086 2304 94-95 0.26663931151424336 2304 96-97 0.2937939724322547 2304 98-99 0.24483245278447896 2304 100-101 0.21319563474331105 2202 1 0.08691900766346805 2202 2 0.06421093965424518 2202 3 0.0788527514866999 2202 4 0.08794874594642721 2202 5 0.04720733602604099 2202 6 -0.009070743600133824 2202 7 0.10591198150662962 2202 8 0.05053538456632367 2202 9 0.11494956765575637 2202 10-11 0.1354509095674743 2202 12-13 0.09435344157915893 2202 14-15 0.016310956038466884 2202 16-17 -0.09168955414885005 2202 18-19 0.023574864415820684 2202 20-21 0.10599429673354166 2202 22-23 0.15986266923012238 2202 24-25 0.12496924536729637 2202 26-27 0.0772650171106406 2202 28-29 0.04047198732177293 2202 30-31 0.13598880161360682 2202 32-33 0.12404001766865491 2202 34-35 0.12907622967612298 2202 36-37 0.11881306792891877 2202 38-39 0.14831657581087399 2202 40-41 0.12668317073657676 2202 42-43 0.2308541679933498 2202 44-45 0.09981302932892788 2202 46-47 0.1823880605641932 2202 48-49 0.21088871679161514 2202 50-51 0.06509091108171816 2202 52-53 0.22700776631343444 2202 54-55 0.24660365624003333 2202 56-57 0.23711469936807106 2202 58-59 0.24869727670746755 2202 60-61 0.16794160298862693 2202 62-63 0.15393172622855644 2202 64-65 0.10392669291417889 2202 66-67 0.03255515808216103 2202 68-69 0.07110183682480908 2202 70-71 0.1605452968196346 2202 72-73 0.05035776161960115 2202 74-75 0.26457915569585566 2202 76-77 0.14547178675774575 2202 78-79 0.21873659926134792 2202 80-81 0.22022878144544933 2202 82-83 0.21142401118022036 2202 84-85 0.3369280038817237 2202 86-87 0.17032455242090094 2202 88-89 0.09398017152148697 2202 90-91 -0.006324128264644457 2202 92-93 0.22134025856972528 2202 94-95 0.14847673938738026 2202 96-97 0.15427341924939597 2202 98-99 0.408363822512964 2202 100-101 0.4485036118553971 1102 1 0.07247997925948368 1102 2 0.038232774963645966 1102 3 0.049867259687040644 1102 4 0.10207751317892644 1102 5 0.1089520802913242 1102 6 0.12013805950371648 1102 7 0.11520755968274443 1102 8 0.09470566135424718 1102 9 0.13293235205100018 1102 10-11 0.0465866636982355 1102 12-13 0.0748338465238163 1102 14-15 0.09125885326496075 1102 16-17 0.16924675246209375 1102 18-19 0.1593983269816519 1102 20-21 0.1771733360813954 1102 22-23 0.2052522479080281 1102 24-25 0.17589614928481723 1102 26-27 0.06293139863404207 1102 28-29 0.15722387366918866 1102 30-31 0.0727957100246357 1102 32-33 0.2145741422407994 1102 34-35 0.07148995500791244 1102 36-37 0.10346409285660485 1102 38-39 0.14109370426539414 1102 40-41 0.18218101788003338 1102 42-43 0.152302562213265 1102 44-45 0.22013270598370838 1102 46-47 0.13921544770469296 1102 48-49 0.12420216115105376 1102 50-51 -0.027633000813914066 1102 52-53 0.1062728981802934 1102 54-55 0.04940054719074283 1102 56-57 0.05430361591388788 1102 58-59 -0.0037340794738511818 1102 60-61 -0.11455412465854664 1102 62-63 -0.09437292189092972 1102 64-65 -0.18655285084522433 1102 66-67 0.1455872144241397 1102 68-69 0.13019022960373405 1102 70-71 -0.004296431613269647 1102 72-73 -0.06133300935223218 1102 74-75 -0.05853850970766672 1102 76-77 -0.034670315203598534 1102 78-79 0.21218077324719786 1102 80-81 0.24995768501806737 1102 82-83 0.20917363180040383 1102 84-85 0.23433904440453546 1102 86-87 0.18871517411351846 1102 88-89 0.11232437660806482 1102 90-91 0.10690308032913265 1102 92-93 0.1978260109631833 1102 94-95 -0.09772328069272973 1102 96-97 0.2870251789770073 1102 98-99 0.09850974225348352 1102 100-101 0.203474292121399 2305 1 0.03207576068707141 2305 2 0.015263867738006809 2305 3 0.06109478601578644 2305 4 0.040920606855941344 2305 5 0.0814910986144497 2305 6 0.046976498811851286 2305 7 0.05391281042529528 2305 8 0.07963850468721034 2305 9 0.10746360674647804 2305 10-11 0.06362286515386728 2305 12-13 0.11803337421810767 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52-53 -0.2990921000316149 1213 54-55 -0.3089848204276464 1213 56-57 -0.3500013443725365 1213 58-59 -0.16162402404590637 1213 60-61 -0.3257347531919308 1213 62-63 -0.18215912877317209 1213 64-65 -0.165505658645408 1213 66-67 -0.09765797410621246 1213 68-69 -0.2874440177528612 1213 70-71 -0.1540575567918836 1213 72-73 -0.13728154611040821 1213 74-75 -0.09928843342815696 1213 76-77 -0.12681886141201204 1213 78-79 -0.21059362633630485 1213 80-81 -0.3672815693999496 1213 82-83 -0.36919500133306826 1213 84-85 -0.342827400474647 1213 86-87 -0.3030494166394391 1213 88-89 -0.24218511288026434 1213 90-91 -0.23783255913001966 1213 92-93 -0.3434548169164202 1213 94-95 -0.21853112800136287 1213 96-97 -0.38328468126819004 1213 98-99 -0.21517983195241186 1213 100-101 0.026999374862842984 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 30.0 9 119.0 10 206.0 11 152.0 12 146.0 13 305.0 14 661.0 15 1372.0 16 2585.0 17 4096.0 18 5926.0 19 7887.0 20 10199.0 21 12976.0 22 16485.0 23 20592.0 24 25303.0 25 30785.0 26 37525.0 27 46410.0 28 60867.0 29 82550.0 30 91545.0 31 95806.0 32 123370.0 33 168198.0 34 245333.0 35 382098.0 36 636084.0 37 998869.0 38 459472.0 39 1656.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.97916652047518 29.228622617979333 16.02123009117577 17.77098077036971 2 31.030952961247866 27.042365972753323 27.489221511593705 14.437459554405102 3 35.057443250102956 22.695084336944372 24.109972798148817 18.137499614803858 4 29.971424056024322 23.71514732387041 26.57913053809528 19.73429808200999 5 34.259260815607306 21.47088897666692 26.346827804718163 17.92302240300761 6 31.593675499564377 22.097596095932047 28.16727877835394 18.141449626149637 7 32.34465389776474 24.25323774866162 23.082409562949454 20.319698790624187 8 29.18626964850502 28.45417286482277 22.82403399811184 19.535523488560376 9 30.618695039514122 23.27565196197904 25.172105636189947 20.933547362316894 10-11 25.64729760393993 29.98746361647351 27.89894414235729 16.46629463722928 12-13 24.506360638837297 29.44507943444802 27.449301184162977 18.599258742551708 14-15 23.93879163269937 29.54848008605982 27.60347769084018 18.90925059040063 16-17 24.92249853625466 28.1078605225781 27.658469692767557 19.311171248399685 18-19 23.00838186804721 28.15401402394099 29.17831359728374 19.659290510728063 20-21 24.068861864461383 28.32351433349862 29.07334414683957 18.53427965520043 22-23 22.704623194130452 31.27167953922137 27.468911169567544 18.554786097080633 24-25 22.300587459134192 28.613223853586245 29.07143917682884 20.01474951045072 26-27 22.277951933124346 28.454831200047064 27.77898706021106 21.48822980661753 28-29 23.36980790618583 29.920481509184476 27.69944055513067 19.010270029499022 30-31 21.892139477421903 28.5688072366449 29.151279831690296 20.3877734542429 32-33 21.90799555133474 29.855222279184563 27.732091180829276 20.504690988651422 34-35 21.70508823092719 29.811239883348602 29.260927664366697 19.222744221357516 36-37 21.765557021635416 28.42295096663221 29.155790128333347 20.65570188339903 38-39 22.89346735357644 29.89012805320469 27.811553643115072 19.404850950103793 40-41 21.49296421737949 31.040239690050175 27.924227016396745 19.54256907617359 42-43 21.522771395194436 29.730558800541235 29.046254352716407 19.70041545154793 44-45 21.383134291981477 28.4167878283622 29.196536876577557 21.00354100307877 46-47 22.692180388969827 28.36252210851694 27.80103196190843 21.144265540604803 48-49 21.330390792489936 29.7701134129575 29.020872102429557 19.878623692123007 50-51 22.511974007222644 28.376443121049462 29.085554796368342 20.026028075359555 52-53 21.289482943906123 28.431725757777805 27.77193042418076 22.506860874135317 54-55 22.32770639035138 28.39841132157514 29.297982548744052 19.975899739329428 56-57 21.13993098230092 28.369614112526616 29.18964232984677 21.30081257532569 58-59 21.028452422346255 28.617352758315096 29.092433038084593 21.261761781254055 60-61 22.25072314556062 28.439840592804266 29.10237499912452 20.207061262510592 62-63 20.97495625454894 28.517845846801553 29.028209442517944 21.47898845613156 64-65 22.156728701608895 28.53713341131478 29.07268384593294 20.233454041143386 66-67 20.980481022145792 30.813086436184182 27.728282378982687 20.478150162687335 68-69 20.879440664943147 30.96919128884575 27.681436144486483 20.46993190172462 70-71 20.807169685862505 30.908272198231145 27.737699764080592 20.546858351825758 72-73 20.802478250721705 30.909178533072158 27.626816035667446 20.661527180538695 74-75 20.66636712607899 30.814348302376835 27.863635338335857 20.65564923320832 76-77 20.760993213823404 30.547335122806825 27.847798555345726 20.843873108024052 78-79 20.69694423611226 30.573159897606097 27.85810201456679 20.871793851714862 80-81 20.701186363612166 30.360201026536366 27.874025873243685 21.06458673660778 82-83 20.78128475546997 30.394118975274974 27.71272658815514 21.111869681099915 84-85 20.667262920725058 30.285830748359544 27.86014597251862 21.186760358396782 86-87 20.828711371301463 29.996012901052982 27.81854693078839 21.35672879685717 88-89 20.793715392455248 30.174157598512203 27.69899447680222 21.33313253223033 90-91 20.736448118391195 29.950273388487364 27.817117476072557 21.496161017048884 92-93 20.773335347023156 29.957610241462053 27.714316816266727 21.554737595248067 94-95 20.631465335476644 29.912085867584658 27.869769065690992 21.586679731247706 96-97 20.714711020761563 29.75790554673698 27.788072084744265 21.73931134775719 98-99 20.638959237502757 29.858310302189622 27.773016256659766 21.729714203647855 100-101 20.761442099461725 29.718357373274408 27.68454725719398 21.835653270069887 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3.0 1 4.5 2 10.5 3 23.5 4 24.0 5 16.5 6 16.5 7 18.5 8 25.5 9 29.5 10 27.5 11 23.0 12 26.0 13 48.0 14 75.0 15 118.5 16 206.5 17 340.0 18 577.5 19 968.0 20 1596.5 21 2543.5 22 3865.5 23 5810.0 24 8535.5 25 11971.0 26 16108.5 27 21243.0 28 27434.0 29 34490.0 30 42508.0 31 51775.0 32 62183.5 33 73815.5 34 87145.5 35 101938.5 36 117586.5 37 133654.0 38 148831.0 39 160676.0 40 169332.5 41 175201.0 42 177278.0 43 175927.5 44 172141.5 45 168322.5 46 163748.0 47 157710.0 48 150261.0 49 140181.0 50 129052.0 51 117099.0 52 103290.5 53 87943.5 54 72987.5 55 59765.0 56 48594.5 57 39621.0 58 32006.5 59 25619.0 60 20507.0 61 16017.0 62 12176.5 63 9353.0 64 7154.5 65 5354.0 66 3989.5 67 2944.0 68 2213.5 69 1664.0 70 1316.5 71 1066.0 72 826.5 73 625.5 74 451.5 75 343.5 76 273.0 77 231.5 78 182.5 79 130.0 80 102.5 81 72.0 82 48.5 83 43.5 84 38.0 85 29.5 86 22.0 87 15.5 88 14.5 89 8.5 90 5.0 91 8.0 92 3.0 93 0.5 94 1.5 95 1.5 96 1.0 97 0.5 98 0.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19416686977008693 2 0.0 3 0.0 4 0.02448446749084634 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 4.202139729550287E-5 48-49 0.003711890094436087 50-51 8.404279459100574E-5 52-53 0.0026333408971848465 54-55 0.009917049761738678 56-57 0.014721496185857838 58-59 0.01481954611288068 60-61 0.003781925756595258 62-63 0.0026893694269121836 64-65 0.010785491972512403 66-67 0.018139236499225407 68-69 0.026445466031303138 70-71 0.02259350461254871 72-73 0.01795714377761156 74-75 0.02273357593686705 76-77 0.016528416269564465 78-79 0.007199666069962825 80-81 0.003669868697140584 82-83 7.843994161827203E-4 84-85 0.0028714621485260296 86-87 0.01678054465333748 88-89 0.016038166634450263 90-91 0.01949792834511333 92-93 0.019764063861318182 94-95 0.018853600253248955 96-97 0.02115076997206978 98-99 0.030045299066284552 100-101 0.01734082995061085 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3569610.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 93.09738087403056 #Duplication Level Percentage of deduplicated Percentage of total 1 97.68346304521788 90.94074564214938 2 1.6297062127685815 3.0344276000578105 3 0.32894655854038674 0.9187218914290793 4 0.13831591357782638 0.5150739714917759 5 0.05275214985762945 0.24555434936098328 6 0.030807331996005223 0.1720849152326884 7 0.028324839876318474 0.18458778843130846 8 0.014887559744099539 0.11087942558250558 9 0.017664701032880287 0.14800836600755435 >10 0.0706136184553681 1.1888788645764814 >50 0.0036918504090002694 0.223779059766134 >100 7.293328650859406E-4 0.14766807941570192 >500 3.477780246241432E-5 0.02826531586935925 >1k 3.202110032855061E-5 0.03645861541136876 >5k 0.0 0.0 >10k+ 3.0086756156410062E-5 2.104866115217865 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT 75147 2.105187961710103 TruSeq Adapter, Index 13 (97% over 38bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.00355781163768591 0.0 0.0 2 0.004622353702505316 0.0 0.0 3 0.007199666069962825 0.0 0.0 4 0.009636907113101991 0.0 2.801426486366858E-5 5 0.01263443345351453 0.0 5.602852972733716E-5 6 0.021991197917979835 0.0 5.602852972733716E-5 7 0.031600090766218156 0.0 5.602852972733716E-5 8 0.04059266978745577 0.0 5.602852972733716E-5 9 0.045074952165642744 0.0 5.602852972733716E-5 10-11 0.05499200192738142 0.0 5.602852972733716E-5 12-13 0.06839682766464683 0.0 5.602852972733716E-5 14-15 0.08084916839654752 0.0 5.602852972733716E-5 16-17 0.09502438641756383 0.0 5.602852972733716E-5 18-19 0.11313560865192557 0.0 5.602852972733716E-5 20-21 0.13362804339969914 0.0 8.404279459100574E-5 22-23 0.15528307013931494 0.0 8.404279459100574E-5 24-25 0.17684004695190791 0.0 8.404279459100574E-5 26-27 0.19796280265911403 0.0 1.1205705945467433E-4 28-29 0.22155081367432297 0.0 1.1205705945467433E-4 30-31 0.2530248402486546 0.0 1.1205705945467433E-4 32-33 0.2875804359579898 0.0 1.1205705945467433E-4 34-35 0.32657629264821647 0.0 1.400713243183429E-4 36-37 0.3736542647516115 0.0 1.540784567501772E-4 38-39 0.42633508982774027 0.0 1.9609985404568005E-4 40-41 0.488316650838607 0.0 2.521283837730172E-4 42-43 0.5542342160628192 0.0 3.5017831079585724E-4 44-45 0.625586548670583 0.0 3.921997080913601E-4 46-47 0.7031300338132176 0.0 4.062068405231944E-4 48-49 0.7850017228772892 0.0 4.34221105386863E-4 50-51 0.8730225430789358 0.0 4.482282378186973E-4 52-53 0.9639988682236995 0.0 4.482282378186973E-4 54-55 1.0602138608979694 0.0 4.902496351142002E-4 56-57 1.15872602329106 2.801426486366858E-5 5.042567675460345E-4 58-59 1.2599135479786308 2.801426486366858E-5 5.042567675460345E-4 60-61 1.3648129627606376 2.801426486366858E-5 5.042567675460345E-4 62-63 1.4740545885965133 2.801426486366858E-5 5.042567675460345E-4 64-65 1.5862657265079378 2.801426486366858E-5 5.042567675460345E-4 66-67 1.7046960312190969 2.801426486366858E-5 5.32271032409703E-4 68-69 1.8292474528029672 2.801426486366858E-5 5.602852972733716E-4 70-71 1.9532385890895645 2.801426486366858E-5 5.602852972733716E-4 72-73 2.0883653956594697 2.801426486366858E-5 5.602852972733716E-4 74-75 2.2245707514266266 2.801426486366858E-5 5.742924297052059E-4 76-77 2.3694465221690884 2.801426486366858E-5 5.882995621370402E-4 78-79 2.5204153955193984 2.801426486366858E-5 5.882995621370402E-4 80-81 2.6741576810912115 2.801426486366858E-5 5.882995621370402E-4 82-83 2.837354781054513 2.801426486366858E-5 6.163138270007088E-4 84-85 3.0069531405391627 2.801426486366858E-5 6.443280918643773E-4 86-87 3.1873369919963244 2.801426486366858E-5 6.723423567280459E-4 88-89 3.3750885951126315 2.801426486366858E-5 7.28370886455383E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCCGAA 55 4.9701295E-5 43.174885 5 CGAGCGT 50 0.0015995952 38.08031 1 CGGAAGA 22460 0.0 37.096153 4 TCGGAAG 22790 0.0 36.663216 3 GAGCACA 22670 0.0 36.43107 9 AGAGCAC 22815 0.0 36.428513 8 CGTCTTC 11395 0.0 36.350567 52-53 GCCGTCT 11385 0.0 36.256565 50-51 CCGTCTT 11515 0.0 35.703613 52-53 ATCGGAA 23445 0.0 35.69971 2 TGCCGTC 11495 0.0 35.661674 50-51 GAAGAGC 23450 0.0 35.583836 6 GATCGGA 23635 0.0 35.486305 1 ATGCCGT 11600 0.0 35.440006 48-49 CGACGGG 95 8.5641295E-7 35.07397 1 CTGCTTG 11905 0.0 34.934216 58-59 TATGCCG 11760 0.0 34.91744 48-49 TGCTTGA 11880 0.0 34.007843 60-61 AACGCGG 70 2.0288781E-4 34.000275 1 CGTATGC 12080 0.0 33.99057 46-47 >>END_MODULE