FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n01_34211-2.34100000035863.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n01_34211-2.34100000035863.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3569610
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT751472.105187961710103TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGAA554.9701295E-543.1748855
CGAGCGT500.001599595238.080311
CGGAAGA224600.037.0961534
TCGGAAG227900.036.6632163
GAGCACA226700.036.431079
AGAGCAC228150.036.4285138
CGTCTTC113950.036.35056752-53
GCCGTCT113850.036.25656550-51
CCGTCTT115150.035.70361352-53
ATCGGAA234450.035.699712
TGCCGTC114950.035.66167450-51
GAAGAGC234500.035.5838366
GATCGGA236350.035.4863051
ATGCCGT116000.035.44000648-49
CGACGGG958.5641295E-735.073971
CTGCTTG119050.034.93421658-59
TATGCCG117600.034.9174448-49
TGCTTGA118800.034.00784360-61
AACGCGG702.0288781E-434.0002751
CGTATGC120800.033.9905746-47
Produced by FastQC (version 0.11.2)