##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n01_34211-1.3410000003589a.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3899824 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.67969631450035 33.0 33.0 33.0 33.0 33.0 2 32.702047066739425 33.0 33.0 33.0 33.0 33.0 3 32.731884310676584 33.0 33.0 33.0 33.0 33.0 4 36.652374312276656 37.0 37.0 37.0 37.0 37.0 5 36.63837880888984 37.0 37.0 37.0 37.0 37.0 6 36.49125191290684 37.0 37.0 37.0 37.0 37.0 7 36.42652591501565 37.0 37.0 37.0 37.0 37.0 8 36.347388241110366 37.0 37.0 37.0 37.0 37.0 9 36.34348165455672 37.0 37.0 37.0 37.0 37.0 10-11 36.2877015731992 37.0 37.0 37.0 35.0 37.0 12-13 36.259527481240184 37.0 37.0 37.0 33.0 37.0 14-15 37.99350316834811 40.0 37.0 40.0 35.0 40.0 16-17 37.95974677318771 40.0 37.0 40.0 33.0 40.0 18-19 37.94365630346395 40.0 37.0 40.0 33.0 40.0 20-21 37.85210153073575 40.0 37.0 40.0 33.0 40.0 22-23 37.73411300099697 40.0 37.0 40.0 33.0 40.0 24-25 37.67853613393835 40.0 37.0 40.0 33.0 40.0 26-27 37.57394577293745 40.0 37.0 40.0 33.0 40.0 28-29 37.39182768248003 40.0 37.0 40.0 33.0 40.0 30-31 37.31490510853823 40.0 37.0 40.0 33.0 40.0 32-33 37.163948039706405 40.0 37.0 40.0 33.0 40.0 34-35 37.03310675045848 40.0 37.0 40.0 33.0 40.0 36-37 36.93273337463434 40.0 37.0 40.0 33.0 40.0 38-39 36.804804268090045 37.0 37.0 40.0 33.0 40.0 40-41 36.67096040744403 37.0 37.0 40.0 33.0 40.0 42-43 36.51261659500531 37.0 37.0 40.0 33.0 40.0 44-45 36.33369826433193 37.0 37.0 40.0 33.0 40.0 46-47 36.24793247079869 37.0 37.0 40.0 33.0 40.0 48-49 36.02084260725612 37.0 37.0 40.0 30.0 40.0 50-51 35.850679030643434 37.0 37.0 40.0 27.0 40.0 52-53 35.67740646757392 37.0 37.0 40.0 27.0 40.0 54-55 35.45328814838824 37.0 33.0 40.0 27.0 40.0 56-57 35.24407960461805 37.0 33.0 40.0 27.0 40.0 58-59 34.990361744530006 37.0 33.0 40.0 27.0 40.0 60-61 34.74403870533645 37.0 33.0 40.0 27.0 40.0 62-63 34.478721219213995 37.0 33.0 40.0 27.0 40.0 64-65 34.23814908057389 37.0 33.0 38.5 27.0 40.0 66-67 33.89350634797878 37.0 33.0 37.0 27.0 40.0 68-69 33.603483772601024 37.0 33.0 37.0 24.5 40.0 70-71 33.25452199894149 37.0 33.0 37.0 22.0 40.0 72-73 32.9140250944658 37.0 33.0 37.0 22.0 40.0 74-75 32.55370691087598 37.0 33.0 37.0 22.0 38.5 76-77 30.044160710842334 33.0 27.0 35.0 18.5 37.0 78-79 32.28156578348151 35.0 33.0 37.0 22.0 37.0 80-81 32.60340325614695 37.0 33.0 37.0 22.0 37.0 82-83 32.45332673987339 37.0 33.0 37.0 22.0 37.0 84-85 32.2179163726363 33.0 33.0 37.0 22.0 37.0 86-87 32.05839943033327 33.0 33.0 37.0 22.0 37.0 88-89 31.85735061377129 33.0 33.0 37.0 22.0 37.0 90-91 31.657407744554625 33.0 33.0 37.0 22.0 37.0 92-93 31.493507527519192 33.0 33.0 37.0 18.5 37.0 94-95 31.32034663615589 33.0 33.0 37.0 15.0 37.0 96-97 31.123285179023462 33.0 33.0 37.0 15.0 37.0 98-99 30.922005711026962 33.0 27.0 37.0 15.0 37.0 100-101 29.0553481387878 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.07055901938368692 2306 2 0.08081597158712128 2306 3 0.0931527232244278 2306 4 0.15131148790332105 2306 5 0.1584610486209428 2306 6 0.16428132045817279 2306 7 0.2072789165012381 2306 8 0.20146288004485768 2306 9 0.23095648446835781 2306 10-11 0.2630788034944018 2306 12-13 0.20858596804264806 2306 14-15 0.44259863345198625 2306 16-17 0.4794451436611382 2306 18-19 0.34469901444989404 2306 20-21 0.40943382161859887 2306 22-23 0.4077469738883508 2306 24-25 0.343443789267468 2306 26-27 0.36331590777566447 2306 28-29 0.3722669382087318 2306 30-31 0.4501567979534755 2306 32-33 0.4885435265564979 2306 34-35 0.4067092361880711 2306 36-37 0.45091485847201795 2306 38-39 0.36318656383348724 2306 40-41 0.3627132388340968 2306 42-43 0.47475305399805023 2306 44-45 0.2991820047806826 2306 46-47 0.39299355934400637 2306 48-49 0.4460121693613175 2306 50-51 0.23432188472503412 2306 52-53 0.3386674625492887 2306 54-55 0.39315211568797537 2306 56-57 0.4524257616719538 2306 58-59 0.34121017995532554 2306 60-61 0.33371443010770463 2306 62-63 0.22736883578141232 2306 64-65 0.3972196511307402 2306 66-67 0.3481233463098192 2306 68-69 0.3142653641751636 2306 70-71 0.2090273566875851 2306 72-73 0.009360645739441509 2306 74-75 0.14505930015420176 2306 76-77 0.1230158447911407 2306 78-79 0.20301006092720542 2306 80-81 0.22316041755074423 2306 82-83 0.20492867496090383 2306 84-85 0.4593149521323241 2306 86-87 0.3779426647205 2306 88-89 0.19767400323786433 2306 90-91 0.2837527135544491 2306 92-93 0.4192390161753359 2306 94-95 0.2164823224676411 2306 96-97 0.2727457440146104 2306 98-99 0.4069295176066632 2306 100-101 0.4085993369118093 2307 1 0.08792715653904537 2307 2 0.10322395003325369 2307 3 0.10979723451877987 2307 4 0.1657560928433739 2307 5 0.14351737211310223 2307 6 0.18635323505218082 2307 7 0.19477379634236058 2307 8 0.26239901605023164 2307 9 0.16449162358664893 2307 10-11 0.2390413061373451 2307 12-13 0.17629215780009133 2307 14-15 0.4312088880050027 2307 16-17 0.43805381419225853 2307 18-19 0.49471314504167907 2307 20-21 0.44058497289548626 2307 22-23 0.535035623848124 2307 24-25 0.504369179045014 2307 26-27 0.462264731307954 2307 28-29 0.42994257099756084 2307 30-31 0.5058006958754717 2307 32-33 0.5213218939811952 2307 34-35 0.5349912841957902 2307 36-37 0.430332482704344 2307 38-39 0.4823019399409958 2307 40-41 0.5425048876194651 2307 42-43 0.5931782989806393 2307 44-45 0.5843538851123711 2307 46-47 0.5660242956490151 2307 48-49 0.44265557812060763 2307 50-51 0.44185694429333466 2307 52-53 0.4896454041721512 2307 54-55 0.3062987996044271 2307 56-57 0.5292150260507924 2307 58-59 0.5533602559116204 2307 60-61 0.5436518996222617 2307 62-63 0.4148136828604976 2307 64-65 0.35255162932287476 2307 66-67 0.4301414181155536 2307 68-69 0.5024821253597906 2307 70-71 0.31288211234697627 2307 72-73 0.18870018536824773 2307 74-75 0.3404332584155725 2307 76-77 0.4210317003618371 2307 78-79 0.34653242319929234 2307 80-81 0.32994191533764905 2307 82-83 0.42107432402957556 2307 84-85 0.5115014257018373 2307 86-87 0.3173263336641057 2307 88-89 0.30772200538152816 2307 90-91 0.42294678833563637 2307 92-93 0.4730993194003865 2307 94-95 0.42655391048796787 2307 96-97 0.42709548907081896 2307 98-99 0.5816327577339138 2307 100-101 0.46343440673786773 2201 1 0.09399678694673241 2201 2 0.11589338229870805 2201 3 0.10089748780251995 2201 4 0.1529332347563539 2201 5 0.13060796612673897 2201 6 0.2042723219456164 2201 7 0.11850791857290943 2201 8 0.13997819262565514 2201 9 0.1383329144092329 2201 10-11 0.20034574034028907 2201 12-13 0.19605117435369124 2201 14-15 0.3649381124112452 2201 16-17 0.39803445893476663 2201 18-19 0.3707869782678088 2201 20-21 0.3914666796200592 2201 22-23 0.3976239830245305 2201 24-25 0.33802621463521376 2201 26-27 0.3232842786755512 2201 28-29 0.3390651129880453 2201 30-31 0.3380269373296869 2201 32-33 0.38402210976084916 2201 34-35 0.31886360768560706 2201 36-37 0.3196937133105635 2201 38-39 0.39615838975927886 2201 40-41 0.3046506723105793 2201 42-43 0.321069539743128 2201 44-45 0.2769973136680619 2201 46-47 0.47675157993985096 2201 48-49 0.3776073571716907 2201 50-51 0.4540383644263386 2201 52-53 0.4516552037547541 2201 54-55 0.4346621198178937 2201 56-57 0.4972679015719663 2201 58-59 0.39781250278031877 2201 60-61 0.39529581796037405 2201 62-63 0.45719218960763186 2201 64-65 0.3032422750029866 2201 66-67 0.5701636583030307 2201 68-69 0.5017124391549359 2201 70-71 0.18828041299209275 2201 72-73 0.345785147211771 2201 74-75 0.24650006945987712 2201 76-77 0.01999764966443962 2201 78-79 0.26387458966368627 2201 80-81 0.3016022646633232 2201 82-83 0.23367287881079335 2201 84-85 0.26416306865634454 2201 86-87 0.43771931358040206 2201 88-89 0.3479038346500616 2201 90-91 0.3968468923898172 2201 92-93 0.4362532559856973 2201 94-95 0.21818230265748184 2201 96-97 0.2765905935413464 2201 98-99 0.3882132830140961 2201 100-101 0.2509239078875787 1101 1 0.09639397029204844 1101 2 0.05052139642964448 1101 3 0.06563591703729088 1101 4 0.12260795976131789 1101 5 0.11567919581530361 1101 6 0.16626464395183405 1101 7 0.1381407262363652 1101 8 0.15488420185044305 1101 9 0.10011788410778877 1101 10-11 0.2024054399296915 1101 12-13 0.16680782556625218 1101 14-15 0.323095846741694 1101 16-17 0.25727042526504107 1101 18-19 0.3117531804374991 1101 20-21 0.3105289109334777 1101 22-23 0.33137360987974773 1101 24-25 0.33131568620262897 1101 26-27 0.27128300119466786 1101 28-29 0.27780275452238357 1101 30-31 0.3436510707549374 1101 32-33 0.31072807405930547 1101 34-35 0.2535590709005433 1101 36-37 0.39322358247007116 1101 38-39 0.5173592098734048 1101 40-41 0.5224052136538333 1101 42-43 0.3890514238248457 1101 44-45 0.3244791450179392 1101 46-47 0.3473331733708349 1101 48-49 0.3982133502492218 1101 50-51 0.3360043741397192 1101 52-53 0.2999207434099844 1101 54-55 0.3649318625282305 1101 56-57 0.5612788697940303 1101 58-59 0.32452162503059157 1101 60-61 0.3143528654661054 1101 62-63 0.36407211534213246 1101 64-65 0.29967527905530034 1101 66-67 0.48158745960708416 1101 68-69 0.3718771829467258 1101 70-71 0.48722202246307944 1101 72-73 0.2713488267142239 1101 74-75 0.2787920334258587 1101 76-77 0.15096988769297326 1101 78-79 0.31835253554115894 1101 80-81 0.35270525381367435 1101 82-83 0.32576143492967446 1101 84-85 0.4788416457666784 1101 86-87 0.4728854307833146 1101 88-89 0.3071188869975856 1101 90-91 0.39500830901124573 1101 92-93 0.6215669925398224 1101 94-95 0.42360766387056614 1101 96-97 0.4892672675388319 1101 98-99 0.5909862626250479 1101 100-101 0.39765605339407983 2304 1 0.05499923881788504 2304 2 0.07885539734891012 2304 3 0.03306932466976775 2304 4 0.13246116282570597 2304 5 0.06368741050577853 2304 6 0.09954472079242294 2304 7 0.0717576573755494 2304 8 0.15318360323586688 2304 9 0.023566348354023603 2304 10-11 0.11443265294481364 2304 12-13 0.15747666073246336 2304 14-15 0.278040295321631 2304 16-17 0.28629795289950266 2304 18-19 0.23074407194453528 2304 20-21 0.23758728028582965 2304 22-23 0.3242051441683742 2304 24-25 0.2750880869866279 2304 26-27 0.2866770063267552 2304 28-29 0.21704405681227712 2304 30-31 0.285873630261122 2304 32-33 0.31208446042903404 2304 34-35 0.3636050521567924 2304 36-37 0.33298858792730357 2304 38-39 0.2823685340562463 2304 40-41 0.2873478329516459 2304 42-43 0.34258389863085625 2304 44-45 0.40825500242545587 2304 46-47 0.32317810997058416 2304 48-49 0.39233553276001487 2304 50-51 0.37389903262521784 2304 52-53 0.25259056615222875 2304 54-55 0.19812963778604598 2304 56-57 0.28413591628432755 2304 58-59 0.2709730164504478 2304 60-61 0.26701398757116834 2304 62-63 0.3156538750685556 2304 64-65 0.26028846129400307 2304 66-67 0.2713660477256852 2304 68-69 0.3270098230863354 2304 70-71 0.18665169032538387 2304 72-73 0.03191572371145668 2304 74-75 0.15787815072140177 2304 76-77 0.16131199137599594 2304 78-79 0.3199969022599163 2304 80-81 0.30987378934498366 2304 82-83 0.34685167203063827 2304 84-85 0.42609553669699096 2304 86-87 0.29634440024332775 2304 88-89 0.1933131026206425 2304 90-91 0.11181610323537683 2304 92-93 0.30892248542930645 2304 94-95 0.25388685872433925 2304 96-97 0.32875055495750516 2304 98-99 0.416153296061168 2304 100-101 0.31402901984839815 2202 1 0.08332892321395491 2202 2 0.05994688813601812 2202 3 0.08879411474901389 2202 4 0.11223909448837333 2202 5 0.09176062007835384 2202 6 0.032706701741780364 2202 7 0.12623125834424798 2202 8 0.08872429102362389 2202 9 0.09131893330936691 2202 10-11 0.15368699902727911 2202 12-13 0.16203788088423465 2202 14-15 0.20093502561892507 2202 16-17 0.09431187792802831 2202 18-19 0.16372098299284943 2202 20-21 0.18666566132348095 2202 22-23 0.15455676337941782 2202 24-25 0.1468462063577789 2202 26-27 0.128924734192978 2202 28-29 0.1725640847758001 2202 30-31 0.08041315497441559 2202 32-33 0.26145759321460105 2202 34-35 0.34514294514616495 2202 36-37 0.27857891136537205 2202 38-39 0.2866401513864787 2202 40-41 0.3327867196643908 2202 42-43 0.24475318017493208 2202 44-45 0.2573364728189347 2202 46-47 0.28077378673769005 2202 48-49 0.31895215963422885 2202 50-51 0.06121585714047484 2202 52-53 0.3574663274161338 2202 54-55 0.3814833919557046 2202 56-57 0.2987209788797216 2202 58-59 0.35980836621993717 2202 60-61 0.16581732850548292 2202 62-63 0.2603896710265161 2202 64-65 0.061117691030169397 2202 66-67 0.24493107108821022 2202 68-69 0.32231424510089113 2202 70-71 0.16674594093704087 2202 72-73 0.09356479076485869 2202 74-75 0.0940291805843998 2202 76-77 0.009237042049200284 2202 78-79 0.20484776992201148 2202 80-81 0.22633411035820217 2202 82-83 0.3505554987904418 2202 84-85 0.4131127617015764 2202 86-87 0.3233169053650542 2202 88-89 0.23618490950909177 2202 90-91 0.1497880752003482 2202 92-93 0.2998486684777184 2202 94-95 0.10538477855808637 2202 96-97 0.20080192025848476 2202 98-99 0.2643204434238271 2202 100-101 0.19477425019387695 1102 1 0.0644498586431439 1102 2 0.03399343894228224 1102 3 0.017331044378174454 1102 4 0.09001529617234638 1102 5 0.077239980254312 1102 6 0.0824906893291697 1102 7 0.0568161467568018 1102 8 0.05482829220044039 1102 9 0.05538057959188336 1102 10-11 0.10129842885958595 1102 12-13 0.05845598880113556 1102 14-15 0.04544426503107957 1102 16-17 0.06398757035857017 1102 18-19 0.06960156411614804 1102 20-21 -0.044243377049149046 1102 22-23 0.11631687489681042 1102 24-25 0.10285234962120171 1102 26-27 0.13179055432214426 1102 28-29 0.0899143821321644 1102 30-31 0.07324591215789411 1102 32-33 0.21221420580373973 1102 34-35 0.05917665954395801 1102 36-37 0.02509740770182134 1102 38-39 0.08259725186421463 1102 40-41 -0.030497562127173694 1102 42-43 0.07500319243343512 1102 44-45 0.11803622344586984 1102 46-47 0.09894150763599896 1102 48-49 0.1493839918257649 1102 50-51 -0.06117671455427853 1102 52-53 -0.018153515254674346 1102 54-55 -0.005171383958774811 1102 56-57 0.025406173404725507 1102 58-59 0.02346999113763104 1102 60-61 -0.18674509356543467 1102 62-63 -0.10681120388067455 1102 64-65 -0.09358414704545481 1102 66-67 -0.05786824663829293 1102 68-69 0.1197981426758119 1102 70-71 -0.12235396940073429 1102 72-73 -0.11486704843154172 1102 74-75 -0.2084408648771543 1102 76-77 -0.0369568227429653 1102 78-79 0.04902243964279762 1102 80-81 0.14889221568329702 1102 82-83 0.06888800905256431 1102 84-85 0.08993358785492944 1102 86-87 0.06415340839815542 1102 88-89 -0.13278942117640824 1102 90-91 0.00144271101760296 1102 92-93 -0.06767386090767502 1102 94-95 -0.3857686506718032 1102 96-97 -0.13630261153658196 1102 98-99 -0.05305296997507014 1102 100-101 -0.07122783024994916 2305 1 0.0171529603068592 2305 2 0.0697850846901602 2305 3 0.019681763194974167 2305 4 0.025990395714181602 2305 5 0.05013002480738038 2305 6 0.027701629616693424 2305 7 -0.05566218453990501 2305 8 0.07091475861513175 2305 9 0.08108771345000321 2305 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-0.07905240915828671 1213 90-91 0.06073805607839233 1213 92-93 -0.0713433735297393 1213 94-95 0.1132111718532336 1213 96-97 0.01060488264038284 1213 98-99 0.024568751782730658 1213 100-101 0.1746348120316732 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 7.0 8 41.0 9 154.0 10 216.0 11 241.0 12 284.0 13 478.0 14 1204.0 15 2604.0 16 4485.0 17 6944.0 18 9523.0 19 12456.0 20 15295.0 21 18839.0 22 22713.0 23 27511.0 24 32458.0 25 38993.0 26 46808.0 27 56561.0 28 70331.0 29 89709.0 30 105183.0 31 122332.0 32 156088.0 33 211138.0 34 300105.0 35 452842.0 36 712434.0 37 996955.0 38 383700.0 39 1192.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.36762378905986 24.597162571387248 14.977364440346353 16.057849199206544 2 36.9751045175372 21.80180438912115 28.589008119340768 12.63408297400088 3 43.002017526944805 18.998472751590842 22.322443269234714 15.67706645222964 4 35.665631736822604 20.075612718223358 28.24114506587258 16.017610479081466 5 38.04694775969377 17.660950853166707 28.01262313376193 16.27947825337759 6 34.45047776515043 18.37190601422013 30.50163289420241 16.675983326427037 7 40.10163022741539 19.075604437533592 21.442300985890643 19.380464349160373 8 34.444374925637675 26.55473682914921 20.285966751320057 18.71492149389306 9 34.30393269029577 21.674798657580443 23.406851180976375 20.614417471147416 10-11 28.774029289526915 28.636279483381816 27.934811929974273 14.654879297116999 12-13 28.39821745801862 28.078690217814955 26.93071533484588 16.59237698932054 14-15 27.205458502742687 28.69219226303546 27.21290755685385 16.889441677368005 16-17 27.038899704191778 28.099652702275797 27.419391234065948 17.44205635946648 18-19 25.727314873696866 28.39796103619035 27.99439154177214 17.880332548340643 20-21 25.66717113387681 28.430129154546464 28.21217060051941 17.69052911105732 22-23 25.07801634125027 29.7413549944818 27.25287089878928 17.927757765478646 24-25 24.42612794833818 28.778708987892788 28.11320459589971 18.68195846786932 26-27 24.47147614866722 28.41755166386996 27.613515379155572 19.49745680830725 28-29 24.69388362141471 29.324887482101758 27.334656640915078 18.646572255568454 30-31 23.858410020554775 28.559070870890586 28.343330365678042 19.2391887428766 32-33 23.90542496276755 29.12172446756572 27.540999286121632 19.431851283545104 34-35 23.543216309248827 29.198099709115077 28.346035615966258 18.912648365669835 36-37 23.59784697976114 28.34317651258108 28.373370182859535 19.685606324798247 38-39 23.912284246673696 29.266346378708374 27.620823401261184 19.200545973356746 40-41 23.16941738909243 29.588335268463396 27.89354083671468 19.348706505729492 42-43 23.277178662421687 28.933959583817114 28.16756602349234 19.621295730268855 44-45 22.949279249525105 28.514838105514507 28.33740702144507 20.198475623515318 46-47 23.602256001559045 28.21821263545497 27.741280366786157 20.438250996199827 48-49 22.898257277626634 29.04592421752123 28.088536620171762 19.967281884680375 50-51 23.235699717268858 28.337078807267417 28.375913952907545 20.05130752255618 52-53 22.879255921964223 28.38824591183139 27.66862617712626 21.06387198907813 54-55 22.93246059307973 28.519708717298524 28.544519959522415 20.003310730099333 56-57 22.56420659413426 28.288652089488064 28.49380595360386 20.653335362773813 58-59 22.433069632305173 28.71561604713125 28.23000847088368 20.6213058496799 60-61 22.8182771055373 28.48457728984694 28.44444586906002 20.252699735555733 62-63 22.383604530745416 28.555997026959556 28.18841855352633 20.871979888768692 64-65 22.69268003708258 28.641908188556975 28.286879664974528 20.37853210938592 66-67 22.32629288683184 29.24841762108233 27.757912151056903 20.667377341028928 68-69 22.22973880219458 29.597283874208618 27.53814422095479 20.634833102642013 70-71 22.066089058313377 29.413719228582252 27.8020677869509 20.71812392615347 72-73 22.08253716182988 29.422215759239535 27.64242060757936 20.85282647135123 74-75 21.782951591716795 29.572188721041726 27.895673463923178 20.7491862233183 76-77 21.992824415401692 29.17776992145429 27.858959074752494 20.970446588391525 78-79 21.819204549665415 29.459734925111032 27.807424440757682 20.91363608446587 80-81 21.730614089118493 29.327862260883126 27.949294067024272 20.992229582974115 82-83 21.887879304223805 29.412595067655595 27.58245735634125 21.117068271779356 84-85 21.673525289290446 29.456878119780068 27.796038376147585 21.073558214781904 86-87 21.89658873467654 29.005231627559613 27.759196895543642 21.3389827422202 88-89 21.836935466787306 29.39694046266212 27.50044431997501 21.265679750575565 90-91 21.760148240287762 29.064380654385975 27.755531760738123 21.41993934458814 92-93 21.811378552824287 29.091896041190402 27.526430385142486 21.570295020842824 94-95 21.519774768391915 29.17904720258983 27.781069850138394 21.520108178879855 96-97 21.658212852481988 28.894270855641235 27.737572611027 21.70994368084977 98-99 21.51152620295782 29.140783988024673 27.636878309131134 21.710811499886372 100-101 21.64833466802679 28.885160153597678 27.67044591157426 21.79605926680127 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1.0 1 6.0 2 55.0 3 133.5 4 113.5 5 45.0 6 31.0 7 33.5 8 42.0 9 39.5 10 27.0 11 32.0 12 40.0 13 40.5 14 51.0 15 80.5 16 119.5 17 182.0 18 269.0 19 416.5 20 707.0 21 1198.5 22 1894.5 23 2885.5 24 4486.5 25 6801.5 26 9912.0 27 13786.5 28 18610.5 29 24746.0 30 31957.5 31 40769.5 32 51201.5 33 63302.0 34 77104.5 35 92390.5 36 109561.5 37 127410.0 38 143847.5 39 158283.5 40 170962.5 41 181873.5 42 189067.5 43 192730.5 44 194160.5 45 194184.0 46 193442.5 47 190576.5 48 185215.5 49 176574.5 50 165498.0 51 151843.5 52 134617.0 53 114985.5 54 95554.0 55 78360.5 56 63750.5 57 51459.0 58 41208.5 59 33197.0 60 26726.0 61 20909.5 62 16121.0 63 12522.5 64 9618.5 65 7433.5 66 5713.0 67 4309.0 68 3235.5 69 2502.0 70 1954.0 71 1553.0 72 1236.5 73 920.0 74 729.5 75 576.0 76 423.5 77 303.0 78 243.5 79 207.5 80 173.5 81 128.0 82 94.0 83 79.5 84 59.5 85 45.5 86 33.5 87 26.0 88 20.0 89 15.5 90 11.0 91 7.5 92 7.5 93 5.0 94 2.5 95 1.0 96 0.5 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1922394446518612 2 0.0 3 0.0 4 0.025872962472152587 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 1.0256873130684873E-4 48-49 0.003833506332593471 50-51 1.5385309696027308E-4 52-53 0.0032180939447523788 54-55 0.009795313839804053 56-57 0.014244232560238615 58-59 0.015000676953626625 60-61 0.0035001579558462128 62-63 0.00288474556800512 64-65 0.010744074604392403 66-67 0.018218770898379005 68-69 0.02655248031706046 70-71 0.02238562560771973 72-73 0.017834138155978323 74-75 0.02239844669913309 76-77 0.016141754089415317 78-79 0.007295201014199615 80-81 0.004154033617927373 82-83 9.102974903482824E-4 84-85 0.00282064011093834 86-87 0.01679562975149648 88-89 0.015564804975814294 90-91 0.019205994937207423 92-93 0.019500880039714612 94-95 0.0186290458236064 96-97 0.020385535347236184 98-99 0.029283372788105312 100-101 0.01734693668227079 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3899824.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 93.61941780379817 #Duplication Level Percentage of deduplicated Percentage of total 1 96.995244076303 90.80638280160791 2 2.342370578161367 4.385827396164267 3 0.2870282882362335 0.8061426371369087 4 0.13261196316260457 0.4966021914040708 5 0.05575475916200477 0.26098640462689343 6 0.03765131236308595 0.21149363657886328 7 0.03230598348177359 0.21171271555999346 8 0.026632477148313093 0.19946536042384247 9 0.013330022843659377 0.1123154080141249 >10 0.07327415075322277 1.2538616606012698 >50 0.0028450277525980377 0.17211689440723232 >100 7.729591520043308E-4 0.10138373303496245 >500 1.5099074703977915E-4 0.09027063864599473 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 2.7410733074339916E-5 0.8914385217936293 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT 34738 0.8907581470343278 TruSeq Adapter, Index 13 (97% over 38bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 6.923389363212289E-4 0.0 0.0 2 9.744029474150628E-4 0.0 0.0 3 0.00141032005546917 0.0 2.564218282671218E-5 4 0.0020770168089636865 0.0 7.692654848013654E-5 5 0.0026155026483246423 0.0 7.692654848013654E-5 6 0.004692519457288329 0.0 1.282109141335609E-4 7 0.007282379922786259 0.0 1.5385309696027308E-4 8 0.009692745108497203 0.0 1.5385309696027308E-4 9 0.011462055723540344 0.0 1.7949527978698525E-4 10-11 0.01610329081517525 0.0 2.307796454404096E-4 12-13 0.021295832837584464 0.0 2.564218282671218E-4 14-15 0.025321655541378277 0.0 2.6924291968047787E-4 16-17 0.02968082662191935 0.0 2.9488510250719007E-4 18-19 0.03437334607920768 0.0 3.0770619392054616E-4 20-21 0.040194121580871336 0.0 3.333483767472583E-4 22-23 0.04609182363101515 0.0 3.589905595739705E-4 24-25 0.052297231875079495 0.0 3.718116509873266E-4 26-27 0.057566700445968844 0.0 3.974538338140388E-4 28-29 0.06314387521077874 0.0 4.102749252273949E-4 30-31 0.09060665301818749 0.0 4.359171080541071E-4 32-33 0.09950449045905661 0.0 4.359171080541071E-4 34-35 0.10520987613800006 0.0 4.359171080541071E-4 36-37 0.1126461091577466 0.0 4.615592908808192E-4 38-39 0.12040286946282705 0.0 4.615592908808192E-4 40-41 0.12987765601729717 0.0 4.615592908808192E-4 42-43 0.14122432191811735 0.0 4.615592908808192E-4 44-45 0.15359667513200595 0.0 5.384858393609557E-4 46-47 0.16727677967005689 0.0 5.641280221876679E-4 48-49 0.18343135485088558 0.0 5.897702050143801E-4 50-51 0.20218861158862553 0.0 6.154123878410923E-4 52-53 0.22244593602172816 0.0 6.410545706678045E-4 54-55 0.24478027726379448 0.0 6.666967534945167E-4 56-57 0.26869161274970355 0.0 7.17981119147941E-4 58-59 0.2947056072274031 0.0 7.820865762147215E-4 60-61 0.32377102146148135 0.0 7.949076676280776E-4 62-63 0.35528526415551065 0.0 7.949076676280776E-4 64-65 0.389145766578184 2.564218282671218E-5 8.077287590414336E-4 66-67 0.42586537238603583 2.564218282671218E-5 8.205498504547898E-4 68-69 0.4639311927922901 2.564218282671218E-5 8.718342161082142E-4 70-71 0.5055228133372173 2.564218282671218E-5 8.718342161082142E-4 72-73 0.5523710813616204 2.564218282671218E-5 8.846553075215702E-4 74-75 0.6031297822670971 2.564218282671218E-5 9.231185817616384E-4 76-77 0.6577348105965808 2.564218282671218E-5 9.231185817616384E-4 78-79 0.7169938951091126 2.564218282671218E-5 9.231185817616384E-4 80-81 0.7789838720926894 2.564218282671218E-5 9.359396731749945E-4 82-83 0.8455894419850742 2.564218282671218E-5 9.744029474150628E-4 84-85 0.9178106499165091 2.564218282671218E-5 9.872240388284189E-4 86-87 0.9986860945519593 5.128436565342436E-5 0.0010385084044818433 88-89 1.0853182092320064 5.128436565342436E-5 0.0011282560443753359 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCGCCG 50 0.0015984289 38.086052 1 ACGTCGG 50 0.0016158636 38.00204 2 TCGGAAG 9520 0.0 37.722614 3 CGGAAGA 9510 0.0 37.56248 4 CGTATGC 4805 0.0 36.573757 46-47 TGCCGTC 4810 0.0 36.537613 50-51 TATGCCG 4825 0.0 36.471848 48-49 ATGCCGT 4780 0.0 36.467415 48-49 GCCGTCT 4835 0.0 36.25045 50-51 CTCGTAT 4775 0.0 36.15699 44-45 CGTCTTC 4850 0.0 35.994434 52-53 TCTCGTA 4790 0.0 35.746296 42-43 GATCGGA 10110 0.0 35.693897 1 ATCGGAA 10070 0.0 35.662296 2 CCGTCTT 5030 0.0 35.084118 52-53 TCGTATG 5010 0.0 34.93502 44-45 ATCTCGT 4930 0.0 34.875698 42-43 AATCTCG 4930 0.0 34.679012 40-41 CGACGGG 155 3.6379788E-11 33.78601 1 GAGCACA 10710 0.0 33.212646 9 >>END_MODULE