FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n01_34211-1.3410000003589a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n01_34211-1.3410000003589a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3899824
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT347380.8907581470343278TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCCG500.001598428938.0860521
ACGTCGG500.001615863638.002042
TCGGAAG95200.037.7226143
CGGAAGA95100.037.562484
CGTATGC48050.036.57375746-47
TGCCGTC48100.036.53761350-51
TATGCCG48250.036.47184848-49
ATGCCGT47800.036.46741548-49
GCCGTCT48350.036.2504550-51
CTCGTAT47750.036.1569944-45
CGTCTTC48500.035.99443452-53
TCTCGTA47900.035.74629642-43
GATCGGA101100.035.6938971
ATCGGAA100700.035.6622962
CCGTCTT50300.035.08411852-53
TCGTATG50100.034.9350244-45
ATCTCGT49300.034.87569842-43
AATCTCG49300.034.67901240-41
CGACGGG1553.6379788E-1133.786011
GAGCACA107100.033.2126469
Produced by FastQC (version 0.11.2)