##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n01_34139-1.341000000356ec.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2829985 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.69283194080534 33.0 33.0 33.0 33.0 33.0 2 32.709539096496975 33.0 33.0 33.0 33.0 33.0 3 32.74546331517658 33.0 33.0 33.0 33.0 33.0 4 36.66141516651148 37.0 37.0 37.0 37.0 37.0 5 36.66016639664168 37.0 37.0 37.0 37.0 37.0 6 36.53406890849245 37.0 37.0 37.0 37.0 37.0 7 36.40242156760548 37.0 37.0 37.0 37.0 37.0 8 36.35201458664975 37.0 37.0 37.0 37.0 37.0 9 36.407099684273945 37.0 37.0 37.0 37.0 37.0 10-11 36.32485949572171 37.0 37.0 37.0 37.0 37.0 12-13 36.321671492958444 37.0 37.0 37.0 35.0 37.0 14-15 38.155550471115575 40.0 37.0 40.0 37.0 40.0 16-17 38.17071132885863 40.0 37.0 40.0 37.0 40.0 18-19 38.12103173691733 40.0 37.0 40.0 37.0 40.0 20-21 38.111710486097984 40.0 37.0 40.0 37.0 40.0 22-23 37.894382655738454 40.0 37.0 40.0 33.0 40.0 24-25 37.98137604969638 40.0 37.0 40.0 33.0 40.0 26-27 37.914909796341675 40.0 37.0 40.0 33.0 40.0 28-29 37.70487882444607 40.0 37.0 40.0 33.0 40.0 30-31 37.63952070417334 40.0 37.0 40.0 33.0 40.0 32-33 37.56165474375306 40.0 37.0 40.0 33.0 40.0 34-35 37.42932718724657 40.0 37.0 40.0 33.0 40.0 36-37 37.39034800537812 40.0 37.0 40.0 33.0 40.0 38-39 37.358823986699576 40.0 37.0 40.0 33.0 40.0 40-41 37.28989181921459 40.0 37.0 40.0 33.0 40.0 42-43 36.86178796000686 37.0 37.0 40.0 33.0 40.0 44-45 36.691811970734825 37.0 37.0 40.0 33.0 40.0 46-47 36.900939404272464 37.0 37.0 40.0 33.0 40.0 48-49 36.64363309346163 37.0 37.0 40.0 33.0 40.0 50-51 36.56018424125923 37.0 37.0 40.0 33.0 40.0 52-53 36.47401116967051 37.0 37.0 40.0 33.0 40.0 54-55 36.195014284528014 37.0 37.0 40.0 33.0 40.0 56-57 36.07310268428984 37.0 37.0 40.0 33.0 40.0 58-59 35.87030991330343 37.0 37.0 40.0 27.0 40.0 60-61 35.594864107053574 37.0 35.0 40.0 27.0 40.0 62-63 35.23953572192079 37.0 33.0 38.5 27.0 40.0 64-65 34.98016968287818 37.0 33.0 37.0 27.0 40.0 66-67 34.40743078143524 37.0 33.0 37.0 27.0 40.0 68-69 34.18320839156391 37.0 33.0 37.0 27.0 40.0 70-71 33.73540248446547 37.0 33.0 37.0 27.0 40.0 72-73 32.81485573245088 37.0 33.0 37.0 22.0 37.0 74-75 32.120556999418724 37.0 33.0 37.0 18.5 37.0 76-77 29.609013652015825 33.0 27.0 35.0 18.5 37.0 78-79 31.656009837507973 33.0 33.0 37.0 15.0 37.0 80-81 31.93919791094299 37.0 33.0 37.0 15.0 37.0 82-83 31.827889017079595 37.0 33.0 37.0 15.0 37.0 84-85 31.61811423028744 33.0 33.0 37.0 15.0 37.0 86-87 31.492407556930516 33.0 33.0 37.0 15.0 37.0 88-89 31.351678895824534 33.0 33.0 37.0 15.0 37.0 90-91 31.202505313632408 33.0 33.0 37.0 15.0 37.0 92-93 31.07482407150568 33.0 33.0 37.0 15.0 37.0 94-95 30.94680219153105 33.0 33.0 37.0 15.0 37.0 96-97 30.778621264777023 33.0 33.0 37.0 15.0 37.0 98-99 30.60553783853978 33.0 30.0 37.0 15.0 37.0 100-101 28.991836705848264 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.094928980041324 2306 2 0.09959732974591162 2306 3 0.0676378768807453 2306 4 0.12912758374979205 2306 5 0.092897651023371 2306 6 0.15704362272842332 2306 7 0.23162439466190676 2306 8 0.218543773944063 2306 9 0.26266061951967146 2306 10-11 0.2517845053971328 2306 12-13 0.19146661737703852 2306 14-15 0.3727827941004307 2306 16-17 0.3756091199517826 2306 18-19 0.3424358304662789 2306 20-21 0.39747444489178463 2306 22-23 0.43337380033982953 2306 24-25 0.2851544659534895 2306 26-27 0.45597397423781416 2306 28-29 0.34938003767560133 2306 30-31 0.4176154405237398 2306 32-33 0.47563667809085075 2306 34-35 0.4758787535319726 2306 36-37 0.5046390477954859 2306 38-39 0.6587179871118991 2306 40-41 0.6598587674083589 2306 42-43 0.6627160595885684 2306 44-45 0.5494483099692857 2306 46-47 0.5761990115348894 2306 48-49 0.5274779674429624 2306 50-51 0.6200078984053832 2306 52-53 0.5679066233668522 2306 54-55 0.6163567380757087 2306 56-57 0.6865807564826056 2306 58-59 0.5121427210544098 2306 60-61 0.49711104157504593 2306 62-63 0.4365840974727959 2306 64-65 0.3862513656960971 2306 66-67 0.43658153373544195 2306 68-69 0.6614222224486994 2306 70-71 0.5996145590187751 2306 72-73 0.3412649392117544 2306 74-75 0.4276889146266072 2306 76-77 0.48833967392639366 2306 78-79 0.6107454239018004 2306 80-81 0.6051429467746772 2306 82-83 0.5914697321767655 2306 84-85 0.700012904712441 2306 86-87 0.704879267709952 2306 88-89 0.6965790391954734 2306 90-91 0.6819006805004477 2306 92-93 0.714854560312137 2306 94-95 0.6812324349318644 2306 96-97 0.687738790806872 2306 98-99 0.7384315365185863 2306 100-101 0.5231212200685214 2307 1 0.07214771588916591 2307 2 0.030421767433260527 2307 3 0.059565374670306426 2307 4 0.16877305182251234 2307 5 0.1116108553967976 2307 6 0.12998231285803286 2307 7 0.1528022860164171 2307 8 0.16631651984128126 2307 9 0.18720677983538536 2307 10-11 0.25832436867375463 2307 12-13 0.1807655326473636 2307 14-15 0.5227655419385187 2307 16-17 0.4398733152462526 2307 18-19 0.45759239383562544 2307 20-21 0.4201306308700907 2307 22-23 0.43463072816108905 2307 24-25 0.418251875972679 2307 26-27 0.40395471651208226 2307 28-29 0.46584938274040866 2307 30-31 0.4841977872490659 2307 32-33 0.5425826141284631 2307 34-35 0.650973532596467 2307 36-37 0.5846985677540815 2307 38-39 0.5903616868380226 2307 40-41 0.600272927120244 2307 42-43 0.5717873541476663 2307 44-45 0.6299047374778581 2307 46-47 0.7303374816994292 2307 48-49 0.6382603529856468 2307 50-51 0.5963917374427155 2307 52-53 0.5818379597624386 2307 54-55 0.6131113907681254 2307 56-57 0.6276785331102488 2307 58-59 0.701664620517434 2307 60-61 0.5009389728251747 2307 62-63 0.5949387803565003 2307 64-65 0.44643725774069054 2307 66-67 0.7112865217667519 2307 68-69 0.6931135812720939 2307 70-71 0.6756007357240463 2307 72-73 0.38779660771145785 2307 74-75 0.5238468581689162 2307 76-77 0.6349459580263677 2307 78-79 0.5364726025263771 2307 80-81 0.6120054254790332 2307 82-83 0.7177546947611368 2307 84-85 0.6429458800634187 2307 86-87 0.7933118009742834 2307 88-89 0.6064114775729088 2307 90-91 0.5344447274263295 2307 92-93 0.721311936689613 2307 94-95 0.6815703616533071 2307 96-97 0.6725087979233884 2307 98-99 0.8052481891678731 2307 100-101 0.826062929326465 2201 1 0.099802258891458 2201 2 0.11726197184213305 2201 3 0.06383518240008357 2201 4 0.14970188336501877 2201 5 0.14858128804227277 2201 6 0.19560055979714264 2201 7 0.24085155994529828 2201 8 0.23436669292944146 2201 9 0.1773292153581707 2201 10-11 0.20971371963060648 2201 12-13 0.19821341887373478 2201 14-15 0.39585070730890237 2201 16-17 0.34427200247369427 2201 18-19 0.35260505699557143 2201 20-21 0.48278464936279875 2201 22-23 0.47708093721562506 2201 24-25 0.41418108216488747 2201 26-27 0.4093412800751466 2201 28-29 0.4185281281134863 2201 30-31 0.4706915156130478 2201 32-33 0.47094359660984253 2201 34-35 0.444906005734083 2201 36-37 0.4863596146752087 2201 38-39 0.5955243597388602 2201 40-41 0.5597631038050466 2201 42-43 0.45900160881955543 2201 44-45 0.5587695488791979 2201 46-47 0.6114713215591578 2201 48-49 0.5474878252990365 2201 50-51 0.658396562930875 2201 52-53 0.5157686345814909 2201 54-55 0.7032410195651977 2201 56-57 0.6633604704987874 2201 58-59 0.5318107339014233 2201 60-61 0.5924341248580234 2201 62-63 0.727076831278886 2201 64-65 0.6019900159118023 2201 66-67 0.7688959569448599 2201 68-69 0.7305120137377443 2201 70-71 0.5501782057895284 2201 72-73 0.31385903933788484 2201 74-75 0.3694003656394287 2201 76-77 0.2629604644628465 2201 78-79 0.3561914653895961 2201 80-81 0.5525518146052022 2201 82-83 0.5219771467685099 2201 84-85 0.6059697527424568 2201 86-87 0.5649864851445834 2201 88-89 0.49550527487243556 2201 90-91 0.5054291569846896 2201 92-93 0.6432433308360856 2201 94-95 0.5006508214234202 2201 96-97 0.4639416082457366 2201 98-99 0.6250039175618234 2201 100-101 0.577781972021544 1101 1 0.07747500986928912 1101 2 0.0699347228199727 1101 3 0.09961310504240828 1101 4 0.14422600331286617 1101 5 0.11342266135822143 1101 6 0.13093302394316453 1101 7 0.1935786351300024 1101 8 0.15553140392466247 1101 9 0.18229444786577886 1101 10-11 0.1913304080269569 1101 12-13 0.1391738708511383 1101 14-15 0.22620164168525747 1101 16-17 0.2442937321564429 1101 18-19 0.30352313609161286 1101 20-21 0.2962298905622802 1101 22-23 0.3461921931912926 1101 24-25 0.29314131684546396 1101 26-27 0.22686626921031205 1101 28-29 0.29734168743551237 1101 30-31 0.2510967741962702 1101 32-33 0.3533316887436939 1101 34-35 0.38861526257893786 1101 36-37 0.29933766769563164 1101 38-39 0.4300582580439425 1101 40-41 0.39022811901582344 1101 42-43 0.5394992185544467 1101 44-45 0.5018758070278295 1101 46-47 0.4041973102597254 1101 48-49 0.5122531447253706 1101 50-51 0.3535834382025058 1101 52-53 0.42111956895784886 1101 54-55 0.4185604459015764 1101 56-57 0.4717589928803605 1101 58-59 0.4250310728844724 1101 60-61 0.5163096137758885 1101 62-63 0.5859099473153933 1101 64-65 0.41756476602045467 1101 66-67 0.5126921325206979 1101 68-69 0.40323582947002734 1101 70-71 0.42516190852747116 1101 72-73 0.15455873512232898 1101 74-75 0.28463830566550286 1101 76-77 0.11736072521904006 1101 78-79 0.32035921854039984 1101 80-81 0.38433690804284026 1101 82-83 0.22185220109271953 1101 84-85 0.3880101699523628 1101 86-87 0.4239391144361946 1101 88-89 0.33521627009190524 1101 90-91 0.39027270326891284 1101 92-93 0.4804065207535153 1101 94-95 0.27607455023697725 1101 96-97 0.40963913106190475 1101 98-99 0.42484637736190933 1101 100-101 0.23973329753219375 2304 1 0.05628340596451409 2304 2 0.04593742146651891 2304 3 0.07662056762622882 2304 4 0.1113176609152049 2304 5 0.13816622109331433 2304 6 0.10796974481223742 2304 7 0.13729223438098614 2304 8 0.12007862335492092 2304 9 0.1358966591037074 2304 10-11 0.12797692663522753 2304 12-13 0.1814662058688299 2304 14-15 0.201080994285455 2304 16-17 0.2592698436270737 2304 18-19 0.2619720683363127 2304 20-21 0.2795232375724268 2304 22-23 0.31077693206970025 2304 24-25 0.3437631414609754 2304 26-27 0.29889500428858895 2304 28-29 0.29864488588241755 2304 30-31 0.2950404812554339 2304 32-33 0.2888481491107697 2304 34-35 0.32121415558310673 2304 36-37 0.3324895422911851 2304 38-39 0.4087016029887991 2304 40-41 0.4375046889809866 2304 42-43 0.42770124529307907 2304 44-45 0.5303590919415768 2304 46-47 0.3189859965971351 2304 48-49 0.43732680676119884 2304 50-51 0.4724770619616123 2304 52-53 0.36560575904719883 2304 54-55 0.35251520226611177 2304 56-57 0.33051203918417826 2304 58-59 0.3378076497339393 2304 60-61 0.3553988549880742 2304 62-63 0.31770337693716755 2304 64-65 0.30350925789009153 2304 66-67 0.14477401441360627 2304 68-69 0.3031010692502818 2304 70-71 0.4268308355400947 2304 72-73 0.0945200453427617 2304 74-75 0.2422976084233497 2304 76-77 0.14063276531321733 2304 78-79 0.48648156636289386 2304 80-81 0.44622076297682156 2304 82-83 0.44990900347193374 2304 84-85 0.3785748892793599 2304 86-87 0.32417111542753574 2304 88-89 0.3188065026436391 2304 90-91 0.40686421630089953 2304 92-93 0.6331029299013728 2304 94-95 0.5049156467686693 2304 96-97 0.48798318095932913 2304 98-99 0.5961493348582287 2304 100-101 0.3486828906699202 2202 1 0.02934527278982557 2202 2 0.02283519534996259 2202 3 0.04651305253907623 2202 4 0.12629544233495693 2202 5 0.10008008384849632 2202 6 0.1320207852818669 2202 7 0.11157617151211241 2202 8 0.0788482682641316 2202 9 0.15570723175398626 2202 10-11 0.1807429092049162 2202 12-13 0.11484530769232038 2202 14-15 0.13369968963829848 2202 16-17 0.09505704982853302 2202 18-19 0.15308005508839528 2202 20-21 0.13677474725511019 2202 22-23 0.1925011416437954 2202 24-25 0.17045869948704961 2202 26-27 0.16036036595106395 2202 28-29 0.20263510028671305 2202 30-31 0.10723814951432331 2202 32-33 0.11168005508233136 2202 34-35 0.16589040696310065 2202 36-37 0.2435742490556052 2202 38-39 0.3084489299519646 2202 40-41 0.20458979966093693 2202 42-43 0.2346705948148653 2202 44-45 0.36349132782049765 2202 46-47 0.26079506069388714 2202 48-49 0.23490866901826024 2202 50-51 0.2506583968056475 2202 52-53 0.29281337356467674 2202 54-55 0.3375074175363153 2202 56-57 0.3157820270344516 2202 58-59 0.30497951192002404 2202 60-61 0.196519391511778 2202 62-63 0.21519368070616451 2202 64-65 0.10331701087445566 2202 66-67 0.13545266347747287 2202 68-69 0.17781563453458915 2202 70-71 0.1718220571522835 2202 72-73 0.2525286062872283 2202 74-75 0.059298548527067396 2202 76-77 -0.10381258962593876 2202 78-79 0.0690156717383914 2202 80-81 0.09598002674075445 2202 82-83 0.08531442561850255 2202 84-85 0.16001142195490203 2202 86-87 0.06679933441169439 2202 88-89 -0.06828765419559701 2202 90-91 -0.02402267509163636 2202 92-93 0.16471593365856307 2202 94-95 -0.08076299906903373 2202 96-97 -0.0708444380876756 2202 98-99 0.12339395345808768 2202 100-101 0.17548200850808016 1102 1 0.013533032318413518 1102 2 0.009968351581548518 1102 3 0.0313244175267684 1102 4 0.066805406338176 1102 5 0.01899964490964834 1102 6 0.024791924334003568 1102 7 0.16323076356106014 1102 8 0.06104100159888759 1102 9 0.06976276045923413 1102 10-11 0.022850954301802062 1102 12-13 0.04076257312750187 1102 14-15 0.008180331703805166 1102 16-17 0.0024487068386136457 1102 18-19 0.015541968839869469 1102 20-21 0.02158262354092244 1102 22-23 0.06266338733320964 1102 24-25 0.07445481199047066 1102 26-27 0.049215437155169184 1102 28-29 0.06506032251852645 1102 30-31 0.0193356442955519 1102 32-33 0.07822002071861789 1102 34-35 -0.012096621080949888 1102 36-37 0.007429508228561588 1102 38-39 0.07980098839133376 1102 40-41 0.04152802467559269 1102 42-43 0.064282621092012 1102 44-45 0.009763725060935258 1102 46-47 0.04624072280889635 1102 48-49 0.022228982075112924 1102 50-51 -0.1645553959576489 1102 52-53 -0.024341430663511687 1102 54-55 0.08625148486344614 1102 56-57 0.06731938667059012 1102 58-59 0.019838509718233865 1102 60-61 -0.14936442148297147 1102 62-63 0.048011327763219924 1102 64-65 -0.023487753248694787 1102 66-67 0.08279574632902609 1102 68-69 0.12908080023739643 1102 70-71 -0.023648329633473963 1102 72-73 0.0074550443015652945 1102 74-75 -0.1202355383458169 1102 76-77 -0.19514304518122927 1102 78-79 -0.0041034593101834105 1102 80-81 0.13591686619942323 1102 82-83 -0.044710846417533645 1102 84-85 0.18509188014175493 1102 86-87 0.1472587994553507 1102 88-89 0.06227250200200629 1102 90-91 -0.02012638156669766 1102 92-93 -0.07160053551048762 1102 94-95 -0.29071608505140034 1102 96-97 0.02742033874517702 1102 98-99 -0.006850415070335458 1102 100-101 0.15004365762948524 2305 1 6.491702601607585E-5 2305 2 -0.012362004794411519 2305 3 0.03575491666079955 2305 4 0.06338559237936892 2305 5 0.046443220395914864 2305 6 -0.021934485854210095 2305 7 -0.05335883473611602 2305 8 0.0573178025697203 2305 9 0.0408620528553314 2305 10-11 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 10.0 10 11.0 11 17.0 12 32.0 13 75.0 14 272.0 15 775.0 16 1632.0 17 2590.0 18 3330.0 19 4264.0 20 5730.0 21 7568.0 22 10138.0 23 13290.0 24 17467.0 25 23350.0 26 31179.0 27 45037.0 28 74972.0 29 115198.0 30 93614.0 31 69051.0 32 86244.0 33 121797.0 34 184361.0 35 312439.0 36 574474.0 37 818810.0 38 211797.0 39 459.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.16301256723216 24.493659033244626 18.314407020572254 15.028921378950958 2 29.493831239388197 28.700222792700313 27.497248218630133 14.308697749281357 3 35.72824590943062 18.537766101233753 28.77577089631217 16.95821709302346 4 29.85323256650731 18.50646711234615 26.93074955164606 24.70955076950048 5 38.0753961593436 16.610688749233653 27.55926268160432 17.754652409818426 6 35.02435525276636 17.44684159103317 29.64135852310171 17.887444633098763 7 32.09472841728843 25.164232319252577 23.011394053325372 19.729645210133622 8 28.716724646950425 31.464548398666427 20.882760862690088 18.935966091693064 9 34.57311611192285 22.18775011174971 23.466202117679067 19.772931658648368 10-11 26.345333985869186 30.047774104809744 27.287441452869892 16.319450456451182 12-13 22.47840536257259 29.262469589061425 26.96781078344938 21.2913142649166 14-15 22.40840499154589 29.495598033205123 27.08645098825612 21.009545986992865 16-17 25.86130315178349 25.634835520329613 27.411470378818265 21.092390949068633 18-19 21.172462044851827 25.63741503930233 31.460608448454675 21.72951446739117 20-21 24.661473470707442 25.816656272029707 31.284141082019868 18.23772917524298 22-23 20.54896050685781 33.48745664729672 27.436046480811736 18.527536365033736 24-25 20.69717684015993 25.19329607754105 31.635697715712276 22.47382936658675 26-27 20.693113214380997 25.34833223497651 27.794864637091717 26.16368991355078 28-29 24.148131527198906 29.938692254552585 27.36585176246517 18.547324455783336 30-31 19.739380244064897 27.074807817002565 30.890216732597526 22.295595206335015 32-33 19.404643487509652 31.23815143896522 27.337636065208827 22.019569008316296 34-35 19.658355079620563 30.990270266450175 31.533824384228186 17.817550269701076 36-37 19.53884914584353 31.142974256047296 31.76232382857153 17.555852769537648 38-39 19.78376210474614 27.12198121191455 31.255024319916892 21.83923236342242 40-41 23.678358719215826 26.567013606079183 27.303289593407737 22.45133808129725 42-43 19.981607676365776 30.32553176076905 30.99880388058594 18.694056682279232 44-45 19.505863105281477 26.54909831677553 31.46711024970097 22.477928328242022 46-47 23.3876711251748 26.354314911841325 27.730100804510275 22.527913158473595 48-49 19.472936458759037 30.92479150665583 30.965906229808155 18.636365804776975 50-51 23.373566818999844 26.960021993080517 30.426794384838622 19.23961680308101 52-53 19.499029119352063 26.7815174007608 26.75455536493275 26.964898114954387 54-55 23.675182700366097 26.531031630014684 30.861718099948 18.93206756967122 56-57 19.90026093946335 26.538075303308773 30.46232775954482 23.099335997683053 58-59 19.766731753001775 26.97106455916525 30.61629396640838 22.645909721424594 60-61 23.210189124627192 27.010297256420767 30.690046927784927 19.089466691167118 62-63 19.43211238101868 27.095257673525484 30.362589461814753 23.11004048364108 64-65 23.367412388967715 26.93466963754044 30.481111921956256 19.21680605153559 66-67 19.978830200715496 34.21672184421113 26.561648204940617 19.242799750132754 68-69 20.22603400989904 34.27164551069721 26.560648536041786 18.94167194336196 70-71 19.786604481383645 34.64976952661948 26.57862425664294 18.985001735353933 72-73 19.657226748527748 34.47935666576781 26.449376906028572 19.414039679675874 74-75 20.043984706964483 33.56858406884163 26.531455073836526 19.855976150357364 76-77 20.319736692834578 32.50426658401504 26.5875244341232 20.588472289027184 78-79 20.059446128315574 32.0891946360665 27.130715399628734 20.720643835989186 80-81 19.65762584471144 31.839686856699025 27.376274631509073 21.126412667080462 82-83 19.32188830130728 31.639193678076538 27.327368234514854 21.71154978610133 84-85 19.390479818383206 31.37953461138137 27.796952876138132 21.433032694097296 86-87 19.545847960284668 31.221274010553667 28.213661811101957 21.019216218059704 88-89 19.84167149662845 31.47415145817724 27.993274572725085 20.69090247246922 90-91 20.28375213577494 30.930721637023666 27.42533822069206 21.360188006509333 92-93 19.949884341684175 31.210109367351663 27.20437328036671 21.635633010597445 94-95 19.666323729562933 31.21751389566772 27.41543584525632 21.70072652951303 96-97 19.789317468614627 31.677942817316463 27.11280709883252 21.41993261523639 98-99 19.98937151998575 31.34562141615748 27.007119703123617 21.65788736073316 100-101 20.106919062520596 31.225257538946043 26.756784313683912 21.911039084849445 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 90.0 1 58.5 2 33.0 3 51.0 4 51.0 5 38.5 6 38.5 7 54.5 8 67.0 9 62.5 10 63.5 11 75.5 12 88.0 13 84.0 14 80.0 15 92.5 16 107.5 17 133.0 18 178.0 19 244.0 20 319.0 21 450.0 22 679.5 23 988.0 24 1485.0 25 2207.5 26 3112.0 27 4340.0 28 5955.0 29 8003.0 30 10542.5 31 13864.5 32 19698.0 33 29643.5 34 44279.5 35 65536.5 36 96153.5 37 135403.0 38 172573.5 39 194978.0 40 205735.0 41 201875.0 42 189243.5 43 181303.5 44 167867.0 45 153753.5 46 148920.0 47 137963.5 48 115887.5 49 95656.0 50 78666.0 51 66040.5 52 56908.5 53 47270.0 54 38562.0 55 30928.0 56 24390.5 57 18804.5 58 14168.0 59 10731.0 60 8348.0 61 6420.0 62 4892.0 63 3524.0 64 2564.0 65 1921.5 66 1392.0 67 1051.5 68 806.0 69 599.5 70 461.0 71 357.0 72 260.0 73 208.0 74 169.0 75 121.0 76 79.5 77 58.5 78 48.5 79 45.5 80 38.0 81 22.5 82 14.0 83 10.0 84 9.0 85 12.0 86 9.5 87 4.0 88 2.5 89 3.0 90 1.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18579603778818615 2 0.0 3 0.0 4 0.026395899624909673 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 8.833969084641791E-5 48-49 0.003480583819348866 50-51 1.4134350535426868E-4 52-53 0.002826870107085373 54-55 0.010017720941983792 56-57 0.014187354349934716 58-59 0.014487709298812539 60-61 0.0037102670155495523 62-63 0.002703194539900388 64-65 0.010247404138184478 66-67 0.01704956033335866 68-69 0.02545949890193764 70-71 0.020883502916093196 72-73 0.016819877137157973 74-75 0.020936506730601046 76-77 0.015477113836292418 78-79 0.006925831762359165 80-81 0.003480583819348866 82-83 8.303930939563284E-4 84-85 0.0024911792818689852 86-87 0.01583047259967809 88-89 0.015017747443891045 90-91 0.017968293118161404 92-93 0.018728014459440598 94-95 0.017879953427314986 96-97 0.019858762502274748 98-99 0.02805668581282233 100-101 0.016307506930248745 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2829985.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.20937453870478 #Duplication Level Percentage of deduplicated Percentage of total 1 91.18323908928814 47.60619881264911 2 3.9853046566936547 4.161405269443266 3 1.4398258598963918 2.2551722276953017 4 0.6920603987959149 1.4452816225656526 5 0.4395467230920204 1.1474229746585827 6 0.28117359016592264 0.880793836761896 7 0.28262161077940545 1.0328848270939817 8 0.18061760385751105 0.7543945702464164 9 0.1470310085612656 0.6908757295300764 >10 1.120615592576852 11.65665541895482 >50 0.11699553801021449 4.327872952415481 >100 0.12052996544036015 12.565315725169507 >500 0.008102290983673271 2.776726285611311 >1k 0.0022004419407532904 1.6502428643410931 >5k 6.781495913796267E-5 0.20060907021044191 >10k+ 6.78149587105695E-5 6.848147812653092 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT 193419 6.8346298655293225 TruSeq Adapter, Index 27 (97% over 39bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT 5666 0.20021307533432153 TruSeq Adapter, Index 27 (97% over 39bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.004735007429368 0.0 0.0 2 0.005088366192753672 0.0 0.0 3 0.005830419595863582 0.0 0.0 4 0.006501801246296358 0.0 0.0 5 0.007809228670823344 0.0 0.0 6 0.009151991971688896 0.0 0.0 7 0.01109546517031009 0.0 3.533587633856716E-5 8 0.012932930739915582 0.0 3.533587633856716E-5 9 0.014664388680505373 0.0 3.533587633856716E-5 10-11 0.024558434055304182 0.0 3.533587633856716E-5 12-13 0.034434811491933705 0.0 3.533587633856716E-5 14-15 0.04402850191785469 0.0 3.533587633856716E-5 16-17 0.053816539663637794 0.0 3.533587633856716E-5 18-19 0.06570706205156565 0.0 3.533587633856716E-5 20-21 0.07422300824916034 0.0 3.533587633856716E-5 22-23 0.08252693918872361 0.0 3.533587633856716E-5 24-25 0.09068952662293263 0.0 3.533587633856716E-5 26-27 0.09952349570757443 0.0 3.533587633856716E-5 28-29 0.10881683118461759 0.0 3.533587633856716E-5 30-31 0.21097284967941526 0.0 7.067175267713433E-5 32-33 0.22491285289498003 0.0 7.067175267713433E-5 34-35 0.2353369364148573 0.0 7.067175267713433E-5 36-37 0.24618505045079744 0.0 8.833969084641791E-5 38-39 0.25996604222283864 0.0 1.060076290157015E-4 40-41 0.27570817513167034 0.0 1.060076290157015E-4 42-43 0.29284607515587535 0.0 1.2367556718498508E-4 44-45 0.31367657425746076 0.0 1.5901144352355222E-4 46-47 0.3366802297538679 0.0 1.7667938169283582E-4 48-49 0.3619100454596049 0.0 1.7667938169283582E-4 50-51 0.3884296206516996 0.0 1.7667938169283582E-4 52-53 0.41956052770597724 0.0 1.7667938169283582E-4 54-55 0.4544193697139738 0.0 1.7667938169283582E-4 56-57 0.49180472688017784 0.0 1.9434731986211942E-4 58-59 0.5336954082795491 0.0 2.12015258031403E-4 60-61 0.577918257517266 0.0 2.12015258031403E-4 62-63 0.625144656243761 0.0 2.4735113436997016E-4 64-65 0.678166138689781 0.0 2.4735113436997016E-4 66-67 0.7323537050549738 0.0 2.826870107085373E-4 68-69 0.7896508285379604 0.0 2.826870107085373E-4 70-71 0.8506935549128352 0.0 2.826870107085373E-4 72-73 0.9168776512949715 0.0 2.826870107085373E-4 74-75 0.9873020528377359 0.0 2.826870107085373E-4 76-77 1.0654296754223078 0.0 2.826870107085373E-4 78-79 1.1523735991533526 0.0 2.826870107085373E-4 80-81 1.2418087021662658 0.0 2.826870107085373E-4 82-83 1.340060106325652 0.0 2.826870107085373E-4 84-85 1.4483645673033603 0.0 2.826870107085373E-4 86-87 1.5621990929280543 0.0 3.1802288704710444E-4 88-89 1.6828181068097534 0.0 3.7102670155495524E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 28155 0.0 75.0725 3 CGGAAGA 28140 0.0 75.011215 4 GATCGGA 28420 0.0 74.542946 1 GAAGAGC 28555 0.0 73.94744 6 ATCGGAA 28710 0.0 73.621254 2 GGAAGAG 30635 0.0 69.05072 5 TCGGACG 100 0.0 66.512314 3 GAGCACA 35150 0.0 60.032555 9 AGAGCAC 35145 0.0 60.01407 8 AAGAGCA 35545 0.0 59.191734 7 GTCGCGC 25 0.004981944 57.115536 1 TCGCCGG 45 1.514878E-5 52.787544 2 ATCGGAC 145 0.0 45.87056 2 GCCGTCT 23485 0.0 45.02194 50-51 CGTCTTC 23520 0.0 44.93673 52-53 ATGCCGT 23485 0.0 44.85934 48-49 CGGACGA 170 0.0 44.714157 4 TCTCGTA 22945 0.0 44.60776 42-43 TATGCCG 23525 0.0 44.510487 48-49 TGCCGTC 23540 0.0 44.503094 50-51 >>END_MODULE