FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n01_34139-1.341000000356ec.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n01_34139-1.341000000356ec.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2829985
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT1934196.8346298655293225TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT56660.20021307533432153TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG281550.075.07253
CGGAAGA281400.075.0112154
GATCGGA284200.074.5429461
GAAGAGC285550.073.947446
ATCGGAA287100.073.6212542
GGAAGAG306350.069.050725
TCGGACG1000.066.5123143
GAGCACA351500.060.0325559
AGAGCAC351450.060.014078
AAGAGCA355450.059.1917347
GTCGCGC250.00498194457.1155361
TCGCCGG451.514878E-552.7875442
ATCGGAC1450.045.870562
GCCGTCT234850.045.0219450-51
CGTCTTC235200.044.9367352-53
ATGCCGT234850.044.8593448-49
CGGACGA1700.044.7141574
TCTCGTA229450.044.6077642-43
TATGCCG235250.044.51048748-49
TGCCGTC235400.044.50309450-51
Produced by FastQC (version 0.11.2)