##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n01_34098-1.34100000035707.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2385241 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.676335850339655 33.0 33.0 33.0 33.0 33.0 2 32.69760833391678 33.0 33.0 33.0 33.0 33.0 3 32.73441467759442 33.0 33.0 33.0 33.0 33.0 4 36.65843451458364 37.0 37.0 37.0 37.0 37.0 5 36.65376161151011 37.0 37.0 37.0 37.0 37.0 6 36.52752573010442 37.0 37.0 37.0 37.0 37.0 7 36.40045764767585 37.0 37.0 37.0 37.0 37.0 8 36.33774364938386 37.0 37.0 37.0 37.0 37.0 9 36.38460222677708 37.0 37.0 37.0 37.0 37.0 10-11 36.3113693752539 37.0 37.0 37.0 35.0 37.0 12-13 36.30423843963776 37.0 37.0 37.0 35.0 37.0 14-15 38.1270666150716 40.0 37.0 40.0 37.0 40.0 16-17 38.14349744952397 40.0 37.0 40.0 37.0 40.0 18-19 38.10423873310915 40.0 37.0 40.0 37.0 40.0 20-21 38.09484450418218 40.0 37.0 40.0 37.0 40.0 22-23 37.883300681147105 40.0 37.0 40.0 33.0 40.0 24-25 37.964962869580056 40.0 37.0 40.0 33.0 40.0 26-27 37.889990990428224 40.0 37.0 40.0 33.0 40.0 28-29 37.7017777239281 40.0 37.0 40.0 33.0 40.0 30-31 37.62976969622776 40.0 37.0 40.0 33.0 40.0 32-33 37.554872023413985 40.0 37.0 40.0 33.0 40.0 34-35 37.421264350227084 40.0 37.0 40.0 33.0 40.0 36-37 37.369605628949024 40.0 37.0 40.0 33.0 40.0 38-39 37.33537365825927 40.0 37.0 40.0 33.0 40.0 40-41 37.26241646022352 40.0 37.0 40.0 33.0 40.0 42-43 36.84632077010247 37.0 37.0 40.0 33.0 40.0 44-45 36.674069412692475 37.0 37.0 40.0 33.0 40.0 46-47 36.87146246437991 37.0 37.0 40.0 33.0 40.0 48-49 36.60110907032036 37.0 37.0 40.0 33.0 40.0 50-51 36.49407858576974 37.0 37.0 40.0 33.0 40.0 52-53 36.39806648468645 37.0 37.0 40.0 33.0 40.0 54-55 36.1349970506125 37.0 37.0 40.0 33.0 40.0 56-57 36.000613145589895 37.0 37.0 40.0 33.0 40.0 58-59 35.78043350755752 37.0 37.0 40.0 27.0 40.0 60-61 35.49923005683702 37.0 33.0 40.0 27.0 40.0 62-63 35.14073357786488 37.0 33.0 37.0 27.0 40.0 64-65 34.85112929888426 37.0 33.0 37.0 27.0 40.0 66-67 34.271035924671764 37.0 33.0 37.0 27.0 40.0 68-69 34.03835692074721 37.0 33.0 37.0 27.0 40.0 70-71 33.59505622283031 37.0 33.0 37.0 27.0 37.0 72-73 32.709762661299216 37.0 33.0 37.0 22.0 37.0 74-75 32.044375809404585 35.0 33.0 37.0 22.0 37.0 76-77 29.519683964848838 33.0 27.0 33.0 18.5 37.0 78-79 31.64660132875462 33.0 33.0 37.0 22.0 37.0 80-81 31.981751529510014 35.0 33.0 37.0 18.5 37.0 82-83 31.886322807632435 37.0 33.0 37.0 15.0 37.0 84-85 31.70135785021304 33.0 33.0 37.0 15.0 37.0 86-87 31.591281761465613 33.0 33.0 37.0 15.0 37.0 88-89 31.46984225074112 33.0 33.0 37.0 15.0 37.0 90-91 31.334733261754263 33.0 33.0 37.0 15.0 37.0 92-93 31.20064115114573 33.0 33.0 37.0 15.0 37.0 94-95 31.080857447947608 33.0 33.0 37.0 15.0 37.0 96-97 30.902112197467677 33.0 33.0 37.0 15.0 37.0 98-99 30.724738925752156 33.0 33.0 37.0 15.0 37.0 100-101 29.11828133928605 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.0941493615668989 2306 2 0.08058735811575701 2306 3 0.07646357176930962 2306 4 0.1722613558246593 2306 5 0.16985026213752974 2306 6 0.16615475514569766 2306 7 0.231109916062195 2306 8 0.19517807684766098 2306 9 0.15979132951007813 2306 10-11 0.18873764114817249 2306 12-13 0.22654706702183347 2306 14-15 0.5018040789229659 2306 16-17 0.48880979303439887 2306 18-19 0.42107178534370604 2306 20-21 0.47801499584693374 2306 22-23 0.5604193124931953 2306 24-25 0.5088445566772961 2306 26-27 0.5075209233570135 2306 28-29 0.3935108763383255 2306 30-31 0.39836013357994204 2306 32-33 0.5795038102347547 2306 34-35 0.6298491804265964 2306 36-37 0.5657677973623692 2306 38-39 0.610924348177214 2306 40-41 0.5528238499949794 2306 42-43 0.56749502355526 2306 44-45 0.46969149085887807 2306 46-47 0.4683403188548141 2306 48-49 0.5910780638502189 2306 50-51 0.561880370013121 2306 52-53 0.587490120336625 2306 54-55 0.7163687897034734 2306 56-57 0.6223232703015142 2306 58-59 0.5816893473948639 2306 60-61 0.6690093698235202 2306 62-63 0.6222124167715037 2306 64-65 0.5079943392641368 2306 66-67 0.6644809150702997 2306 68-69 0.599663181708209 2306 70-71 0.5255952292746926 2306 72-73 0.20440787041006558 2306 74-75 0.3417846181521682 2306 76-77 0.2547214858654918 2306 78-79 0.5182777456091188 2306 80-81 0.5182731130962495 2306 82-83 0.4197123606345876 2306 84-85 0.6038173990668163 2306 86-87 0.5232673741364557 2306 88-89 0.49901269081193433 2306 90-91 0.6539897576591862 2306 92-93 0.660791477055966 2306 94-95 0.4505091200742406 2306 96-97 0.6370592902754062 2306 98-99 0.6593318672231305 2306 100-101 0.36780925377547646 2307 1 0.08896014091041593 2307 2 0.07691890259091849 2307 3 0.09120898354843376 2307 4 0.18176039304658076 2307 5 0.1504426829410761 2307 6 0.16626641147752252 2307 7 0.2773076497038076 2307 8 0.2772263312379053 2307 9 0.2324913858390758 2307 10-11 0.24334274304710135 2307 12-13 0.25211283171744725 2307 14-15 0.4971631232626095 2307 16-17 0.4830262012711586 2307 18-19 0.4193679215043389 2307 20-21 0.3927515399332151 2307 22-23 0.5000335649083922 2307 24-25 0.521496485886594 2307 26-27 0.4954729400058895 2307 28-29 0.6171887062813184 2307 30-31 0.4945408683552017 2307 32-33 0.47061045705663673 2307 34-35 0.5454135722501618 2307 36-37 0.5118536979075117 2307 38-39 0.4720182738370795 2307 40-41 0.576470245292569 2307 42-43 0.4969243919398423 2307 44-45 0.5727903517875319 2307 46-47 0.6309522992551564 2307 48-49 0.607757575414162 2307 50-51 0.4960040180582226 2307 52-53 0.49455257481973547 2307 54-55 0.39904399881604746 2307 56-57 0.5987281367499335 2307 58-59 0.7734593919159849 2307 60-61 0.6228506717068925 2307 62-63 0.471280307356551 2307 64-65 0.5002442408651575 2307 66-67 0.6381512325160301 2307 68-69 0.6127685122227433 2307 70-71 0.6772433827912465 2307 72-73 0.2632092175923191 2307 74-75 0.29574391043367854 2307 76-77 0.236885344331224 2307 78-79 0.4444883771774002 2307 80-81 0.3325753759193404 2307 82-83 0.3270542505549976 2307 84-85 0.41677669252677063 2307 86-87 0.30422637534552877 2307 88-89 0.363179817066662 2307 90-91 0.3681642785680843 2307 92-93 0.5211032191775651 2307 94-95 0.2130770860788651 2307 96-97 0.47213257689573496 2307 98-99 0.5907041736246015 2307 100-101 0.28219462544081253 2201 1 0.1150858373115966 2201 2 0.09561687309471978 2201 3 0.05147206106062896 2201 4 0.13894679947261324 2201 5 0.14134436891256286 2201 6 0.15970884120657303 2201 7 0.14883643428591853 2201 8 0.18536335155942396 2201 9 0.25458393166266546 2201 10-11 0.23858953523014037 2201 12-13 0.21452625884129617 2201 14-15 0.3437354571279059 2201 16-17 0.32720512297542115 2201 18-19 0.3147202944145775 2201 20-21 0.269194006183902 2201 22-23 0.4984481939260519 2201 24-25 0.43566870642414557 2201 26-27 0.4843673194368989 2201 28-29 0.4853034096703013 2201 30-31 0.49495325848594973 2201 32-33 0.5138704722850562 2201 34-35 0.5195276182497182 2201 36-37 0.5751227273395187 2201 38-39 0.5283935679469138 2201 40-41 0.5030208100498612 2201 42-43 0.46853905770048243 2201 44-45 0.5396544381822252 2201 46-47 0.5058726156874513 2201 48-49 0.3536167966843635 2201 50-51 0.5837193020305378 2201 52-53 0.6871483113061743 2201 54-55 0.6470664352739348 2201 56-57 0.7299033365675456 2201 58-59 0.6505357298805521 2201 60-61 0.6843889353134145 2201 62-63 0.6872085268493677 2201 64-65 0.6478872396676891 2201 66-67 0.7799592083984663 2201 68-69 0.8332772918024958 2201 70-71 0.5423662172242913 2201 72-73 0.38718578176968066 2201 74-75 0.46496912054374917 2201 76-77 0.22920971400100143 2201 78-79 0.4718966193357659 2201 80-81 0.5525081097076097 2201 82-83 0.6509531509217616 2201 84-85 0.6652254914076572 2201 86-87 0.6777883359597148 2201 88-89 0.5630805722898025 2201 90-91 0.6058963292911024 2201 92-93 0.7488228082759676 2201 94-95 0.7010620214577052 2201 96-97 0.6339593890882078 2201 98-99 0.659436230987918 2201 100-101 0.6385180975637361 1101 1 0.048278335075679024 1101 2 0.004543175965181945 1101 3 0.06877159559820001 1101 4 0.12467531671691745 1101 5 0.15133908105049443 1101 6 0.22626722638489127 1101 7 0.25952718574987443 1101 8 0.28167137931743724 1101 9 0.19133666209267375 1101 10-11 0.21108938834508706 1101 12-13 0.2672100773305388 1101 14-15 0.3772975829790184 1101 16-17 0.36027032035628537 1101 18-19 0.37227456028305994 1101 20-21 0.389179121831404 1101 22-23 0.37676795943221464 1101 24-25 0.3609589186567419 1101 26-27 0.4408450810391855 1101 28-29 0.31477155519748834 1101 30-31 0.4156666628898904 1101 32-33 0.39754925751299197 1101 34-35 0.4812386659645185 1101 36-37 0.4924635202577008 1101 38-39 0.47963412650182846 1101 40-41 0.47316932716360327 1101 42-43 0.5411279558014144 1101 44-45 0.5270026079944046 1101 46-47 0.5071833526587497 1101 48-49 0.5505358662817699 1101 50-51 0.6944537926005196 1101 52-53 0.738013758065236 1101 54-55 0.6027275159562677 1101 56-57 0.7273438807603654 1101 58-59 0.5499877506925301 1101 60-61 0.6142418544091228 1101 62-63 0.5161074111757529 1101 64-65 0.5792488315100215 1101 66-67 0.6077356297562062 1101 68-69 0.6053854514306494 1101 70-71 0.5402612564262768 1101 72-73 0.3273196103776712 1101 74-75 0.40588516104485706 1101 76-77 0.27456285832046845 1101 78-79 0.5462473547402809 1101 80-81 0.6477679656949746 1101 82-83 0.2922285049147568 1101 84-85 0.5255759265138416 1101 86-87 0.5562254789474217 1101 88-89 0.4029262450560154 1101 90-91 0.5539082889989011 1101 92-93 0.5921267428370385 1101 94-95 0.28540120190436724 1101 96-97 0.389144039926105 1101 98-99 0.6023405076279715 1101 100-101 0.5616015086917479 2304 1 0.05299862907322961 2304 2 0.05918430271201203 2304 3 0.014626559244646842 2304 4 0.09470975050957264 2304 5 0.12104343799179418 2304 6 0.09330013046393049 2304 7 0.04428880029860949 2304 8 0.013544394803503224 2304 9 0.08719449014311209 2304 10-11 0.017221041228076217 2304 12-13 0.0864699640951585 2304 14-15 0.11275805115671034 2304 16-17 0.08855856472042234 2304 18-19 0.17942118729499867 2304 20-21 0.25012193735769017 2304 22-23 0.2290031087164195 2304 24-25 0.09289129434464627 2304 26-27 0.1717772312314665 2304 28-29 0.17079454356313306 2304 30-31 0.0990622614589114 2304 32-33 0.10503632198646073 2304 34-35 0.07659906283682716 2304 36-37 0.10719667360082497 2304 38-39 0.1766524647818457 2304 40-41 0.24383258904934735 2304 42-43 0.06229212271693996 2304 44-45 0.1535840332486984 2304 46-47 0.15898128419340196 2304 48-49 0.17006269112041394 2304 50-51 0.1367295920434799 2304 52-53 -0.01690698326689244 2304 54-55 0.0018172449348909936 2304 56-57 0.039503458535030234 2304 58-59 0.15612135380803238 2304 60-61 0.21508776775785776 2304 62-63 0.1976187194770418 2304 64-65 0.04755023994355412 2304 66-67 0.08025161252102464 2304 68-69 0.04572418243873244 2304 70-71 0.18009548666341146 2304 72-73 0.0828669857913269 2304 74-75 0.2471558594109311 2304 76-77 0.01770005108266659 2304 78-79 0.26080547471615034 2304 80-81 0.22592962778062287 2304 82-83 0.41896253586250154 2304 84-85 0.22652532407482795 2304 86-87 0.37623218287165017 2304 88-89 0.391598441444426 2304 90-91 0.3732645903829557 2304 92-93 0.43479466090289876 2304 94-95 0.3305041071413477 2304 96-97 0.4517866049694099 2304 98-99 0.4045755959674082 2304 100-101 0.3519721138123266 2202 1 0.10713423968308433 2202 2 0.11732407850038129 2202 3 0.1270638597513809 2202 4 0.14720933052374363 2202 5 0.15409215679856203 2202 6 0.11376909137169378 2202 7 0.14336965061115592 2202 8 0.10987045333668988 2202 9 0.13195169329420509 2202 10-11 0.20512439089093704 2202 12-13 0.1517216876134242 2202 14-15 0.24974515612795045 2202 16-17 0.17269219813157122 2202 18-19 0.1612898472542028 2202 20-21 0.20858635205958365 2202 22-23 0.17925224025778164 2202 24-25 0.19128384080093497 2202 26-27 0.178736047398381 2202 28-29 0.25066976836252053 2202 30-31 0.24521811876370947 2202 32-33 0.20251841122342285 2202 34-35 0.12328482290759268 2202 36-37 0.21349666373735232 2202 38-39 0.1287965994151179 2202 40-41 0.12973088356908136 2202 42-43 0.191700003497715 2202 44-45 0.22139249088507285 2202 46-47 0.2603374893770791 2202 48-49 0.27539750202320334 2202 50-51 0.014949464647031618 2202 52-53 0.0882876446977221 2202 54-55 0.11714096280709896 2202 56-57 0.17548552502745451 2202 58-59 0.1410919412882663 2202 60-61 0.07671358357752212 2202 62-63 0.19096312632417778 2202 64-65 0.10256827118509904 2202 66-67 0.25790235365527536 2202 68-69 0.2508834325084379 2202 70-71 0.10992283533001768 2202 72-73 -0.1808412166107587 2202 74-75 -0.13868399740205106 2202 76-77 -0.21778228856944537 2202 78-79 -0.09618754833960708 2202 80-81 -0.008926448817582866 2202 82-83 -0.21422138910663335 2202 84-85 -0.059338876152800424 2202 86-87 -0.14766174559699508 2202 88-89 -0.31847610660353 2202 90-91 -0.1646719527981162 2202 92-93 -0.1262417785540606 2202 94-95 -0.1500656368542046 2202 96-97 0.04618196724490886 2202 98-99 0.023138721977119303 2202 100-101 0.12853807545827678 1102 1 0.07827329012980044 1102 2 0.06177304204479839 1102 3 0.034964037352004596 1102 4 0.08410730167381075 1102 5 0.11614485063405766 1102 6 0.07959368024657465 1102 7 0.09479342130298107 1102 8 0.13468963284889668 1102 9 0.11478052560396179 1102 10-11 0.09840937550340101 1102 12-13 0.01703224298821482 1102 14-15 0.14747587595278588 1102 16-17 0.15138880778979313 1102 18-19 0.11895039957860121 1102 20-21 0.009772361603921809 1102 22-23 0.11158267232893593 1102 24-25 0.11878512027112009 1102 26-27 0.077667223765296 1102 28-29 0.14302141760806109 1102 30-31 0.023599266999227098 1102 32-33 0.11537568844309476 1102 34-35 0.056256896564406134 1102 36-37 -0.0610270090029843 1102 38-39 0.10988797900595415 1102 40-41 0.11993975919442335 1102 42-43 -0.001297057957529546 1102 44-45 0.059631024798662224 1102 46-47 0.09197192356317174 1102 48-49 0.04411949772553925 1102 50-51 -0.07812944900653207 1102 52-53 -0.10323463672470723 1102 54-55 0.08052608502615755 1102 56-57 0.01234502733897358 1102 58-59 0.05299109870207275 1102 60-61 -0.1626746517365305 1102 62-63 -0.08047647251531487 1102 64-65 -0.1661522416875556 1102 66-67 0.06361734870685609 1102 68-69 -0.0020782020274339175 1102 70-71 -0.2535943434636536 1102 72-73 -0.2489655895856373 1102 74-75 -0.48085075896981877 1102 76-77 -0.36018753151626015 1102 78-79 -0.28647336164203097 1102 80-81 -0.028495563932615653 1102 82-83 -0.06576429457158284 1102 84-85 -0.16012956330454742 1102 86-87 -0.11039738841637003 1102 88-89 -0.24180809268018066 1102 90-91 -0.15534368311630686 1102 92-93 0.012734314842177241 1102 94-95 -0.3890107179268547 1102 96-97 -0.15248352840997725 1102 98-99 -0.08468905116857783 1102 100-101 -0.14657978831797536 2305 1 -0.013179279040166136 2305 2 0.020929827288540537 2305 3 0.03712383407702191 2305 4 0.08103347332824029 2305 5 0.11037245274209084 2305 6 0.014999759674388713 2305 7 -0.04427466640640887 2305 8 -0.08388508953017038 2305 9 0.09811621165703599 2305 10-11 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-0.26403318999235026 1213 96-97 -0.2816201170812924 1213 98-99 -0.05254881165093295 1213 100-101 -4.871619212494238E-4 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 21.0 9 79.0 10 57.0 11 23.0 12 30.0 13 66.0 14 178.0 15 464.0 16 891.0 17 1604.0 18 2238.0 19 3128.0 20 4286.0 21 5989.0 22 8113.0 23 11267.0 24 14591.0 25 19123.0 26 25746.0 27 38120.0 28 63650.0 29 93785.0 30 74550.0 31 57996.0 32 73983.0 33 105749.0 34 161719.0 35 280105.0 36 524541.0 37 685280.0 38 127809.0 39 59.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.87747824870635 24.364431252974487 20.252677510424615 14.505412987894548 2 29.88318580805881 27.698333208258617 28.785896267924286 13.63258471575828 3 34.659851981414036 18.16726276296609 30.606089699112164 16.566795556507707 4 29.807484244974912 17.51294537195614 28.852327986325793 23.827242396743152 5 36.46407218390092 16.219031955261542 30.23388412323954 17.083011737598003 6 33.10114156179606 17.112275027974114 32.85969845395078 16.92688495627905 7 31.69738403792321 24.466458525574563 25.39286386574774 18.443293570754484 8 28.98713379486601 30.908868328189897 21.954804566917975 18.149193310026117 9 33.3007021093466 23.343762747663654 24.186193344823437 19.169341798166307 10-11 25.949159854287263 29.355146922260683 27.974007657926393 16.721685565525664 12-13 22.24783575328447 28.504415277114553 27.609432338283636 21.638316631317338 14-15 22.11977741452541 28.702466543213035 27.97008771859951 21.207668323662055 16-17 25.268746428557954 25.39971851900919 28.458361230584245 20.87317382184861 18-19 20.737506189102064 25.434369105679465 32.10769897046043 21.720425734758038 20-21 24.05979102321317 25.771986981608986 31.794187673279133 18.374034321898712 22-23 20.366348725348928 32.652025518595394 28.302129638053348 18.67949611800233 24-25 20.66399160504117 24.689161388723406 32.18982903614352 22.45701797009191 26-27 20.394186583242533 24.825499813226422 28.852807745632415 25.927505857898637 28-29 23.481715264830683 29.217362103032773 28.172499131115053 19.12842350102149 30-31 19.28740114730545 26.857894024125862 31.22988410814672 22.624820720421962 32-33 19.03906146171393 30.547835627511017 28.180569594435113 22.232533316339943 34-35 19.547647386574354 30.477402493081414 31.96163406548856 18.013316054855675 36-37 19.158085074003004 31.14261829307814 32.23525421540213 17.46404241751672 38-39 19.525469334125987 27.133589436036022 31.542389217693305 21.798552012144683 40-41 23.22721267997657 26.3633108771818 27.94134848428314 22.468127958558483 42-43 19.90476014792635 30.1162230567058 31.356139694060264 18.622877101307584 44-45 19.429776697616717 26.78593064600181 31.599008234388055 22.18528442199342 46-47 23.047837134978927 26.594693445814027 28.275845732859473 22.081623686347573 48-49 19.57799798713249 30.8653799994927 31.100920296777417 18.455701716597396 50-51 23.00348414088079 27.18719925676133 30.46146577079464 19.347850831563242 52-53 19.24531015183979 26.7890222891075 27.453207380495844 26.512460178556868 54-55 23.335805026782968 26.692633074696502 31.0610734696727 18.910488428847827 56-57 19.90607135616634 26.735217538881685 30.409068238000366 22.949642866951613 58-59 19.740323997492553 26.829231475599553 30.761962266682602 22.668482260225293 60-61 22.827988203736265 27.095434188537297 30.775226169123943 19.30135143860249 62-63 19.3620032328736 27.16391541199433 30.55966949307276 22.914411862059307 64-65 23.368454330893215 27.077773393884176 30.400571406289245 19.153200868933368 66-67 20.276956725747468 33.814013928974724 26.792876422595818 19.11615292268199 68-69 20.585171539077344 33.68912072672467 26.799481845512297 18.92622588868569 70-71 20.008898360345015 34.50393737768424 26.74443716193464 18.742727100036106 72-73 19.84566072531534 34.4749332337028 26.61049450040276 19.068911540579105 74-75 20.61915717616758 33.196202724063454 26.493373721201678 19.691266378567285 76-77 20.70160416864967 32.14840855752293 26.763539326808534 20.38644794701887 78-79 20.51836835661697 31.81576718600634 27.32843342780955 20.337431029567142 80-81 19.77258534390901 31.753130871596163 27.628729914594775 20.84555386990005 82-83 19.08377920378061 31.596367130606712 27.816446435593832 21.50340723001884 84-85 19.247391960120286 31.36196648250808 28.040613414753018 21.350028142618616 86-87 19.165977766647288 31.50854323381375 28.741565735737883 20.58391326380108 88-89 19.44306013971822 31.73126937866464 28.57598407293642 20.24968640868072 90-91 20.15478115126868 31.13154993119929 27.756384062053353 20.95728485547868 92-93 19.542862725891236 31.712035008738766 27.531390788904282 21.21371147646571 94-95 19.475250163169264 31.669618354796757 27.63620833418072 21.218923147853264 96-97 19.87696771975142 32.30423442387849 27.051125070238264 20.767672786131822 98-99 20.118336867545366 31.558007731862027 27.09884072393579 21.224814676656816 100-101 20.10971830540023 31.627247089892403 26.731750148700044 21.531284456007324 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 66.0 1 42.0 2 32.5 3 66.0 4 71.5 5 53.0 6 52.5 7 51.5 8 54.0 9 61.0 10 60.0 11 63.0 12 60.5 13 60.5 14 65.0 15 69.0 16 84.5 17 95.0 18 102.0 19 123.5 20 150.0 21 191.5 22 268.0 23 376.0 24 540.0 25 730.0 26 1091.5 27 1789.0 28 2583.0 29 3398.0 30 4420.5 31 5910.5 32 10140.5 33 19250.5 34 34799.0 35 56803.0 36 88744.0 37 129377.0 38 162973.5 39 180660.5 40 187653.0 41 178079.0 42 166018.5 43 165156.0 44 156551.5 45 141424.0 46 130804.0 47 114135.0 48 90218.5 49 73529.0 50 61803.0 51 49847.0 52 38823.5 53 30675.5 54 23751.5 55 16823.5 56 12152.0 57 9520.5 58 7632.0 59 5922.0 60 4478.5 61 3480.0 62 2696.0 63 1978.0 64 1524.5 65 1210.0 66 901.5 67 685.5 68 606.5 69 519.0 70 363.0 71 260.5 72 170.5 73 100.0 74 76.5 75 59.5 76 41.5 77 26.5 78 16.0 79 9.0 80 6.0 81 5.0 82 2.5 83 2.5 84 2.0 85 1.0 86 1.5 87 1.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19193029132066738 2 0.0 3 0.0 4 0.02536431329161288 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 8.384896955905084E-5 48-49 0.0036264679334289487 50-51 1.886601815078644E-4 52-53 0.002620280298720339 54-55 0.009915140650357762 56-57 0.014484909491326034 58-59 0.014505871733715795 60-61 0.0033120342975825083 62-63 0.0023687333900431866 64-65 0.010397272225322305 66-67 0.017943679485636882 68-69 0.02616087850242386 70-71 0.022303825902707526 72-73 0.01802752845519593 74-75 0.022282863660317762 76-77 0.016434398033573966 78-79 0.006749842049503594 80-81 0.0034587699943108476 82-83 7.96565210810983E-4 84-85 0.0028718272073974915 86-87 0.016790756154199935 88-89 0.015491097126034645 90-91 0.019054678332294305 92-93 0.019494885422479322 94-95 0.018761206938837625 96-97 0.02100416687454224 98-99 0.029032705709821357 100-101 0.017042303062877084 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2385241.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.515758904421205 #Duplication Level Percentage of deduplicated Percentage of total 1 84.03235062413718 28.16408003690371 2 6.646118171387911 4.4549938856506 3 2.4406893702663885 2.4540466948329573 4 1.4366233785336036 1.9259809116554925 5 0.7796603634725285 1.3065454384739337 6 0.6165848349401313 1.239918520318547 7 0.49150269124875884 1.1531159990537303 8 0.31952556934904325 0.8567313556880362 9 0.3375867130922852 1.0183027396803341 >10 2.259538270166139 15.336665096745847 >50 0.30557018516528245 7.269863190549758 >100 0.3092613454055697 21.207345562000093 >500 0.019293590861848692 4.2312612482513146 >1k 0.005568910501245292 2.984309110619859 >5k 0.0 0.0 >10k+ 1.2598147201237832E-4 6.396840209575881 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT 151499 6.3515175196133224 TruSeq Adapter, Index 27 (97% over 39bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT 4572 0.19167874441199023 TruSeq Adapter, Index 27 (97% over 39bp) TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 3079 0.1290854886361588 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 3043 0.12757620718409587 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 2798 0.11730470841311214 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 2637 0.11055486636360853 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 2558 0.10724283206602603 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0048632402344249495 0.0 0.0 2 0.005072862658322576 0.0 0.0 3 0.005617880960456407 0.0 0.0 4 0.006162899262590237 0.0 0.0 5 0.007252935866857898 0.0 0.0 6 0.008636443864582238 0.0 0.0 7 0.009936102892747526 0.0 0.0 8 0.011529233314369492 0.0 0.0 9 0.013415835129448135 0.0 0.0 10-11 0.02100416687454224 0.0 0.0 12-13 0.027795933408825357 0.0 0.0 14-15 0.03546811412347851 0.0 0.0 16-17 0.04290971017184427 0.0 0.0 18-19 0.05028841949304075 0.0 0.0 20-21 0.05728980845122149 0.0 0.0 22-23 0.06420734843984319 0.0 0.0 24-25 0.07152317103387038 0.0 0.0 26-27 0.07902765380940542 0.0 0.0 28-29 0.08760121094681837 0.0 0.0 30-31 0.14688243242506732 0.0 0.0 32-33 0.15935496664697613 0.0 0.0 34-35 0.16941684299406223 0.0 0.0 36-37 0.1818684149735813 0.0 0.0 38-39 0.1955148347693168 0.0 0.0 40-41 0.21083823395623336 0.0 0.0 42-43 0.22855132877558285 0.0 0.0 44-45 0.24819294989479052 0.0 0.0 46-47 0.2706644737366162 0.0 0.0 48-49 0.29397448727403225 0.0 0.0 50-51 0.3199257433525585 0.0 0.0 52-53 0.34902133578954914 0.0 0.0 54-55 0.3801921902231263 0.0 0.0 56-57 0.41576511555855367 0.0 0.0 58-59 0.4519040214385045 0.0 0.0 60-61 0.48995049137592384 0.0 0.0 62-63 0.5319378628826186 0.0 0.0 64-65 0.5776984380194705 0.0 0.0 66-67 0.6260583312126531 0.0 0.0 68-69 0.676158090524186 0.0 0.0 70-71 0.7294860351637424 0.0 0.0 72-73 0.7870902772508103 0.0 0.0 74-75 0.8484467607256458 0.0 0.0 76-77 0.9136812590425873 0.0 0.0 78-79 0.9853302035307963 0.0 0.0 80-81 1.06186335049582 0.0 0.0 82-83 1.1414779470921388 0.0 0.0 84-85 1.2294355161595831 0.0 0.0 86-87 1.3253587373351372 0.0 0.0 88-89 1.430148987041561 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 21245 0.0 75.401596 3 GATCGGA 21230 0.0 75.35883 1 CGGAAGA 21260 0.0 75.25902 4 GAAGAGC 21275 0.0 75.18703 6 ATCGGAA 21455 0.0 74.575 2 GGAAGAG 22070 0.0 72.67234 5 GAGCACA 28970 0.0 55.248672 9 AGAGCAC 29085 0.0 55.111866 8 AAGAGCA 29125 0.0 54.873108 7 CACGCCG 50 2.7926542E-5 47.600952 1 GGCGACT 40 5.333236E-4 47.600952 1 CGTCTTC 17935 0.0 44.785954 52-53 GCCGTCT 17930 0.0 44.78473 50-51 ATGCCGT 17905 0.0 44.661556 48-49 TCGTATG 18115 0.0 44.417274 44-45 CTGCTTG 18170 0.0 44.390182 58-59 TGCCGTC 17950 0.0 44.165886 50-51 TCTCGTA 17580 0.0 44.10737 42-43 TATGCCG 17955 0.0 44.034534 48-49 GTATGCC 18185 0.0 43.986027 46-47 >>END_MODULE