FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n01_34098-1.34100000035707.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n01_34098-1.34100000035707.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2385241
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT1514996.3515175196133224TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT45720.19167874441199023TruSeq Adapter, Index 27 (97% over 39bp)
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA30790.1290854886361588No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC30430.12757620718409587No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG27980.11730470841311214No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC26370.11055486636360853No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC25580.10724283206602603No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG212450.075.4015963
GATCGGA212300.075.358831
CGGAAGA212600.075.259024
GAAGAGC212750.075.187036
ATCGGAA214550.074.5752
GGAAGAG220700.072.672345
GAGCACA289700.055.2486729
AGAGCAC290850.055.1118668
AAGAGCA291250.054.8731087
CACGCCG502.7926542E-547.6009521
GGCGACT405.333236E-447.6009521
CGTCTTC179350.044.78595452-53
GCCGTCT179300.044.7847350-51
ATGCCGT179050.044.66155648-49
TCGTATG181150.044.41727444-45
CTGCTTG181700.044.39018258-59
TGCCGTC179500.044.16588650-51
TCTCGTA175800.044.1073742-43
TATGCCG179550.044.03453448-49
GTATGCC181850.043.98602746-47
Produced by FastQC (version 0.11.2)