##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n01_34042-1.3410000003584a.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5509837 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.68725935086646 33.0 33.0 33.0 33.0 33.0 2 32.70979177786929 33.0 33.0 33.0 33.0 33.0 3 32.74889910536373 33.0 33.0 33.0 33.0 33.0 4 36.666581969666254 37.0 37.0 37.0 37.0 37.0 5 36.66507285061246 37.0 37.0 37.0 37.0 37.0 6 36.53670353587592 37.0 37.0 37.0 37.0 37.0 7 36.41031340854548 37.0 37.0 37.0 37.0 37.0 8 36.35757881766738 37.0 37.0 37.0 37.0 37.0 9 36.39493890654116 37.0 37.0 37.0 37.0 37.0 10-11 36.33555257623774 37.0 37.0 37.0 37.0 37.0 12-13 36.33298226426662 37.0 37.0 37.0 37.0 37.0 14-15 38.172002456696994 40.0 37.0 40.0 37.0 40.0 16-17 38.190262071273615 40.0 37.0 40.0 37.0 40.0 18-19 38.159117865011254 40.0 37.0 40.0 37.0 40.0 20-21 38.14280277256841 40.0 37.0 40.0 37.0 40.0 22-23 37.91539341363456 40.0 37.0 40.0 33.0 40.0 24-25 38.01859728336791 40.0 37.0 40.0 35.0 40.0 26-27 37.969438660345126 40.0 37.0 40.0 35.0 40.0 28-29 37.77486167013652 40.0 37.0 40.0 33.0 40.0 30-31 37.730393476249844 40.0 37.0 40.0 33.0 40.0 32-33 37.5787940732185 40.0 37.0 40.0 33.0 40.0 34-35 37.489960229313496 40.0 37.0 40.0 33.0 40.0 36-37 37.497537495210835 40.0 37.0 40.0 33.0 40.0 38-39 37.34185602949779 40.0 37.0 40.0 33.0 40.0 40-41 37.08461157743868 38.5 37.0 40.0 33.0 40.0 42-43 36.9121448601837 37.0 37.0 40.0 33.0 40.0 44-45 36.801255463637126 37.0 37.0 40.0 33.0 40.0 46-47 36.92693967171806 37.0 37.0 40.0 33.0 40.0 48-49 36.636112737999326 37.0 37.0 40.0 33.0 40.0 50-51 36.54471076004607 37.0 37.0 40.0 33.0 40.0 52-53 36.43238683830393 37.0 37.0 40.0 33.0 40.0 54-55 36.15778189082545 37.0 37.0 40.0 33.0 40.0 56-57 36.035884909118 37.0 37.0 40.0 33.0 40.0 58-59 35.83678146921588 37.0 37.0 40.0 27.0 40.0 60-61 35.540470525716096 37.0 33.0 40.0 27.0 40.0 62-63 35.286473356652834 37.0 33.0 38.5 27.0 40.0 64-65 34.94891101134208 37.0 33.0 37.0 27.0 40.0 66-67 34.23782010248216 37.0 33.0 37.0 27.0 40.0 68-69 34.02715760919969 37.0 33.0 37.0 27.0 38.5 70-71 33.527681399649396 37.0 33.0 37.0 27.0 37.0 72-73 32.48731922559597 37.0 33.0 37.0 22.0 37.0 74-75 31.72294606900349 35.0 33.0 37.0 18.5 37.0 76-77 29.220610337474593 33.0 27.0 33.0 15.0 37.0 78-79 31.271588070572687 33.0 33.0 37.0 15.0 37.0 80-81 31.575531454015792 33.0 33.0 37.0 15.0 37.0 82-83 31.474397064740753 33.0 33.0 37.0 15.0 37.0 84-85 31.283327256323552 33.0 33.0 37.0 15.0 37.0 86-87 31.169525069434904 33.0 33.0 37.0 15.0 37.0 88-89 31.046839407408967 33.0 33.0 37.0 15.0 37.0 90-91 30.90470298849131 33.0 33.0 37.0 15.0 37.0 92-93 30.763931945718177 33.0 33.0 37.0 15.0 37.0 94-95 30.641181218246565 33.0 33.0 37.0 15.0 37.0 96-97 30.468349789658024 33.0 27.0 37.0 15.0 37.0 98-99 30.287954979430427 33.0 27.0 37.0 15.0 37.0 100-101 28.710368655188894 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.09045324064414473 2306 2 0.0989558142053042 2306 3 0.09183692937284604 2306 4 0.15093558351594538 2306 5 0.16047765291320815 2306 6 0.17181431704310057 2306 7 0.23901857837721963 2306 8 0.18998182425797694 2306 9 0.24241876644462224 2306 10-11 0.22053601148452628 2306 12-13 0.23358278203339466 2306 14-15 0.4070787060406218 2306 16-17 0.41483050777050323 2306 18-19 0.34916898056194157 2306 20-21 0.4554250367915458 2306 22-23 0.4502292955722069 2306 24-25 0.496478412136689 2306 26-27 0.4720163515884721 2306 28-29 0.46970880337230625 2306 30-31 0.37726826934134294 2306 32-33 0.5009851338980766 2306 34-35 0.5626823100753597 2306 36-37 0.5300167482184932 2306 38-39 0.582850342965493 2306 40-41 0.5119901266937461 2306 42-43 0.5249792418327814 2306 44-45 0.4787574452446677 2306 46-47 0.44625752811838737 2306 48-49 0.5127718582197502 2306 50-51 0.41448388902431077 2306 52-53 0.4745547578820819 2306 54-55 0.5454190029967876 2306 56-57 0.4637212322338016 2306 58-59 0.5049814681682037 2306 60-61 0.517548394279352 2306 62-63 0.4911666537860597 2306 64-65 0.4211844829746667 2306 66-67 0.4630422731680426 2306 68-69 0.4596415472651074 2306 70-71 0.4412868230814908 2306 72-73 0.10479634501983526 2306 74-75 0.1430973859949738 2306 76-77 0.28837703336413867 2306 78-79 0.42984155350382025 2306 80-81 0.3445325780161461 2306 82-83 0.34378277115300904 2306 84-85 0.49203571968859805 2306 86-87 0.40998894490221716 2306 88-89 0.32967118999080824 2306 90-91 0.35136957281789805 2306 92-93 0.48586313817117954 2306 94-95 0.4164055758575351 2306 96-97 0.43070231322278474 2306 98-99 0.4805375576136477 2306 100-101 0.40319375086856013 2307 1 0.08872832697203137 2307 2 0.0656993690016563 2307 3 0.11678515456135585 2307 4 0.139998479978928 2307 5 0.1408931009057568 2307 6 0.18695355141708347 2307 7 0.15926454366850606 2307 8 0.23316426395176393 2307 9 0.16153949615297591 2307 10-11 0.21492134212815017 2307 12-13 0.22029731608201075 2307 14-15 0.470067100481657 2307 16-17 0.41808119595489046 2307 18-19 0.42379471950728487 2307 20-21 0.44147645867392526 2307 22-23 0.545441379119957 2307 24-25 0.5297722601546653 2307 26-27 0.46476232921035887 2307 28-29 0.4630167137524239 2307 30-31 0.4776113887853839 2307 32-33 0.5980882516761099 2307 34-35 0.5675618017697417 2307 36-37 0.5483802969975216 2307 38-39 0.49051046674277643 2307 40-41 0.46078594788144045 2307 42-43 0.534076757135054 2307 44-45 0.6557991456834742 2307 46-47 0.5724888753485757 2307 48-49 0.5674057243993289 2307 50-51 0.5520541802840029 2307 52-53 0.502679961679469 2307 54-55 0.5098987284793068 2307 56-57 0.6080719138966657 2307 58-59 0.6354702284169846 2307 60-61 0.6625538992063866 2307 62-63 0.6028996837318772 2307 64-65 0.4279029463738908 2307 66-67 0.7405309440884906 2307 68-69 0.7099500862317214 2307 70-71 0.4877141281772026 2307 72-73 0.41294991193306174 2307 74-75 0.46436810619506375 2307 76-77 0.6100232202773732 2307 78-79 0.6568227244064566 2307 80-81 0.7101224135478823 2307 82-83 0.7920190571987753 2307 84-85 0.7256414359346799 2307 86-87 0.5971581081039865 2307 88-89 0.5538781133202413 2307 90-91 0.6736496894817954 2307 92-93 0.7943863490150882 2307 94-95 0.6332133182675044 2307 96-97 0.815285859601456 2307 98-99 0.7175948137870307 2307 100-101 0.7082358968529796 2201 1 0.12237212779010065 2201 2 0.08552624408569898 2201 3 0.06989475381896426 2201 4 0.18316278611803227 2201 5 0.13217421558812958 2201 6 0.12786955807243316 2201 7 0.27493281804031966 2201 8 0.19191703329468623 2201 9 0.19837300082006948 2201 10-11 0.25029806334350013 2201 12-13 0.2413920673495369 2201 14-15 0.45959775314973683 2201 16-17 0.3970030294888929 2201 18-19 0.4596282882172318 2201 20-21 0.479641627222712 2201 22-23 0.5633979351047671 2201 24-25 0.456903809014392 2201 26-27 0.435835277842628 2201 28-29 0.4610314492210108 2201 30-31 0.5160385653045694 2201 32-33 0.468361455663306 2201 34-35 0.37147592974980626 2201 36-37 0.3437242925721833 2201 38-39 0.41014504743179714 2201 40-41 0.5182578347292051 2201 42-43 0.41338022608868386 2201 44-45 0.4184434302674447 2201 46-47 0.4913314041889123 2201 48-49 0.4974032545396554 2201 50-51 0.5890590824961777 2201 52-53 0.547308673900254 2201 54-55 0.4944061318071391 2201 56-57 0.4978572644649262 2201 58-59 0.5852984290093914 2201 60-61 0.4755119746141574 2201 62-63 0.5600522110897828 2201 64-65 0.5168789713847914 2201 66-67 0.567864821148163 2201 68-69 0.6440346855594967 2201 70-71 0.45199480323475427 2201 72-73 0.06661396662105545 2201 74-75 0.016231459677658933 2201 76-77 -0.00594380377900805 2201 78-79 0.24275850014523925 2201 80-81 0.3132760199384954 2201 82-83 0.263974668105039 2201 84-85 0.23341916565906118 2201 86-87 0.39183118466887024 2201 88-89 0.2824336141034287 2201 90-91 0.3029464926209684 2201 92-93 0.3395796142588843 2201 94-95 0.2268581562735399 2201 96-97 0.1767317710779075 2201 98-99 0.21046194579771438 2201 100-101 0.28507647174853545 1101 1 0.08104471599636298 1101 2 0.08486455689551775 1101 3 0.06587977052937077 1101 4 0.13510635205123833 1101 5 0.12457042041956612 1101 6 0.1309088331324375 1101 7 0.17665544774222042 1101 8 0.14028300238604174 1101 9 0.15020441638205284 1101 10-11 0.17501712666360447 1101 12-13 0.1766700881197849 1101 14-15 0.2978937404094495 1101 16-17 0.3024939116637171 1101 18-19 0.27182688409457967 1101 20-21 0.2569782802070293 1101 22-23 0.2796014207383024 1101 24-25 0.24441250066815456 1101 26-27 0.2047494379485073 1101 28-29 0.22922784695108334 1101 30-31 0.2771190219808801 1101 32-33 0.36854201776096573 1101 34-35 0.23769175192418146 1101 36-37 0.28965099108955883 1101 38-39 0.38009996298256254 1101 40-41 0.4323450428578681 1101 42-43 0.32817398155602007 1101 44-45 0.3614037514661135 1101 46-47 0.3587360202981884 1101 48-49 0.41894846235430094 1101 50-51 0.4268447855668569 1101 52-53 0.41413010807919903 1101 54-55 0.43812346801151847 1101 56-57 0.3940172357544256 1101 58-59 0.4373328367432805 1101 60-61 0.41405917870540776 1101 62-63 0.5307697491648327 1101 64-65 0.37926485838207213 1101 66-67 0.405235567845331 1101 68-69 0.5526209179799295 1101 70-71 0.39766916341688585 1101 72-73 -0.04548593692961589 1101 74-75 0.13038371502162605 1101 76-77 -0.04664878101248249 1101 78-79 0.18908333248043618 1101 80-81 0.2897749096498181 1101 82-83 0.262432600500798 1101 84-85 0.21311994846865545 1101 86-87 0.20886346154570745 1101 88-89 0.20691155412540851 1101 90-91 0.23637403226612363 1101 92-93 0.300904558744449 1101 94-95 0.14091732680939373 1101 96-97 0.27005209496817884 1101 98-99 0.23759672811384291 1101 100-101 0.17371676064789554 2304 1 0.07547246667185448 2304 2 0.10348915745344556 2304 3 0.0886105651364133 2304 4 0.12489824973644659 2304 5 0.14023976664535098 2304 6 0.14164315194951627 2304 7 0.09250375842095337 2304 8 0.12171914763408154 2304 9 0.1297776725934554 2304 10-11 0.2035776385426118 2304 12-13 0.12917640075826853 2304 14-15 0.2483788912640108 2304 16-17 0.21658324199919576 2304 18-19 0.24019529305547138 2304 20-21 0.23950027659702045 2304 22-23 0.2979479781564365 2304 24-25 0.3570928990293041 2304 26-27 0.3434850206172939 2304 28-29 0.35409749767912047 2304 30-31 0.26137396858017325 2304 32-33 0.17350722741449687 2304 34-35 0.2189725107775189 2304 36-37 0.39596708790195834 2304 38-39 0.35317161729476254 2304 40-41 0.39622986054789777 2304 42-43 0.35446343706916394 2304 44-45 0.3677396926145988 2304 46-47 0.33592710880900967 2304 48-49 0.4191285548679886 2304 50-51 0.4417866120571361 2304 52-53 0.3554821773186134 2304 54-55 0.32811352836647956 2304 56-57 0.31123616671086296 2304 58-59 0.32720956141839963 2304 60-61 0.4324925120634546 2304 62-63 0.44182284841373587 2304 64-65 0.36360983799806945 2304 66-67 0.41434584992300927 2304 68-69 0.3743095221620578 2304 70-71 0.3048534380171688 2304 72-73 0.04552083980258459 2304 74-75 0.22051838958563863 2304 76-77 0.11752380338313984 2304 78-79 0.2488044038394399 2304 80-81 0.17771152873633156 2304 82-83 0.17477584639865285 2304 84-85 0.08058451597493388 2304 86-87 0.11469191835544024 2304 88-89 0.11028324823219648 2304 90-91 0.08434518060335705 2304 92-93 0.23603780919797757 2304 94-95 0.24902309463161387 2304 96-97 0.3368715282452932 2304 98-99 0.4097497331882103 2304 100-101 0.2981820836900617 2202 1 0.05642247855704596 2202 2 0.05720815263988044 2202 3 0.04150133562949776 2202 4 0.044020156914008624 2202 5 0.08140377863002612 2202 6 0.08079066090466114 2202 7 0.10640984738011383 2202 8 0.11624028231976524 2202 9 0.09610283909836426 2202 10-11 0.13637806225092675 2202 12-13 0.07217823981559945 2202 14-15 0.1322892359288943 2202 16-17 0.034868756577516535 2202 18-19 0.0786618794454057 2202 20-21 0.12469425616047403 2202 22-23 0.17160371718137668 2202 24-25 0.10094963752995767 2202 26-27 0.09313011714549191 2202 28-29 0.18898875573781027 2202 30-31 0.16413099624786298 2202 32-33 0.1314580952171056 2202 34-35 0.18060542520937872 2202 36-37 0.19548962483326449 2202 38-39 0.20137718915744784 2202 40-41 0.256487845761427 2202 42-43 0.10190276479792715 2202 44-45 0.08074953602672252 2202 46-47 0.24807877210680118 2202 48-49 0.22523814848113233 2202 50-51 0.17205374986032496 2202 52-53 0.2739545116371289 2202 54-55 0.36206053694210283 2202 56-57 0.3377667601499965 2202 58-59 0.2708806573607987 2202 60-61 0.1872195609594911 2202 62-63 0.25946274623626664 2202 64-65 0.24244689030166455 2202 66-67 0.2221247020133319 2202 68-69 0.2968262242752431 2202 70-71 0.1137995335530988 2202 72-73 0.12207303018841031 2202 74-75 0.02273433328970853 2202 76-77 0.057403287933762925 2202 78-79 0.058864480162320376 2202 80-81 0.1777742715693087 2202 82-83 0.00566828009544551 2202 84-85 0.08133166467380804 2202 86-87 0.042140699241205226 2202 88-89 -0.0686005246861221 2202 90-91 0.0034851555940278445 2202 92-93 0.10805000441700585 2202 94-95 -0.15510872716793145 2202 96-97 -0.021602592109115193 2202 98-99 -0.02572757332493225 2202 100-101 0.16911349079349947 1102 1 0.07091313441673464 1102 2 0.06475066699705678 1102 3 0.06922475505788839 1102 4 0.0855654809941413 1102 5 0.08241285658215958 1102 6 0.06628021150899599 1102 7 0.08504660551979981 1102 8 0.06830840382294667 1102 9 0.10265162203351252 1102 10-11 0.08512195966181224 1102 12-13 0.09394513465581156 1102 14-15 0.08313390763761674 1102 16-17 0.13055164686625886 1102 18-19 0.056107232391838124 1102 20-21 0.008430164874077661 1102 22-23 0.09419015160737132 1102 24-25 0.04071858331185041 1102 26-27 0.06418935165621065 1102 28-29 0.07367898319387933 1102 30-31 0.007935756819975381 1102 32-33 0.037669651232747015 1102 34-35 0.017637265030316485 1102 36-37 0.02575828807768943 1102 38-39 0.10646661735576401 1102 40-41 0.07938635972465846 1102 42-43 0.018781314582220432 1102 44-45 0.11272411868502985 1102 46-47 0.14760150810014494 1102 48-49 0.03923009443030878 1102 50-51 -0.00854506651238296 1102 52-53 -0.03877247912935644 1102 54-55 -0.004174376317955364 1102 56-57 0.0026829050963996792 1102 58-59 0.07037946235876547 1102 60-61 -0.06383351837190787 1102 62-63 -0.015547269336536829 1102 64-65 -0.1554160007465626 1102 66-67 0.04004320156215613 1102 68-69 0.06239345884055325 1102 70-71 -0.05150417667545781 1102 72-73 -0.06305272917598614 1102 74-75 -0.052706113214100014 1102 76-77 -0.15847539181645942 1102 78-79 0.04518256899963191 1102 80-81 0.05935421079443515 1102 82-83 -0.02876126779969823 1102 84-85 0.07459804760696542 1102 86-87 0.003873298074495324 1102 88-89 1.1587885385466734E-5 1102 90-91 0.07207734383526443 1102 92-93 0.020108130620503317 1102 94-95 -0.18923511569646934 1102 96-97 -0.007593346435104564 1102 98-99 0.09849441318660368 1102 100-101 0.16183618365959873 2305 1 0.06387012909247147 2305 2 0.06856093569395938 2305 3 0.050715988465221074 2305 4 0.05209466372549798 2305 5 0.07053727864980175 2305 6 -0.01082037764779642 2305 7 0.03579317828229733 2305 8 0.04301154234649829 2305 9 0.045323162928347926 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0.19906542509170322 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 7.0 9 24.0 10 39.0 11 36.0 12 58.0 13 111.0 14 375.0 15 1009.0 16 1928.0 17 3229.0 18 5063.0 19 7297.0 20 10096.0 21 13994.0 22 18937.0 23 25674.0 24 34130.0 25 45244.0 26 61642.0 27 90069.0 28 148996.0 29 240544.0 30 217847.0 31 140087.0 32 164629.0 33 235290.0 34 364230.0 35 632922.0 36 1199395.0 37 1579950.0 38 266957.0 39 27.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.24911959329729 24.641459404918347 20.532973588071673 14.576447413712687 2 28.059940793166838 29.6427280879634 28.5974884556476 13.699842663222162 3 32.84302239794027 18.159103436272254 31.991490855355615 17.00638331043187 4 27.229587721452873 17.841839436534315 28.989119950678415 25.93945289133439 5 36.911128223938384 16.86576209060268 29.180718050279893 17.042391635179044 6 32.91910450345446 17.700977361036273 32.49464548588279 16.885272649626476 7 29.641094645812572 26.93132301372981 25.362147736856826 18.065434603600796 8 26.759230808461304 32.70786413463774 22.62762038151038 17.90528467539058 9 34.5924570908359 22.9480291340742 23.765403586349287 18.694110188740613 10-11 26.236665440375095 30.008963967536605 26.838634246348846 16.91573634573945 12-13 21.70647697926454 28.825825518976334 26.434021550909765 23.033675950849364 14-15 21.292018257527403 29.15404756982829 26.92192164668392 22.632012525960388 16-17 25.2742685491422 25.19971098963545 27.580861285007163 21.945159176215196 18-19 20.015719158298147 25.341384872184058 32.1348254040909 22.50807056542689 20-21 24.42817273904836 25.758720267042385 31.752926629226963 18.060180364682292 22-23 19.73810659008606 34.79886973062906 27.24757556348763 18.215448115797255 24-25 19.975028299385265 25.115252955758944 31.846450267040566 23.063268477815225 26-27 20.05178193111702 25.178385494888506 26.964382067926874 27.805450506067604 28-29 24.13888105945784 30.020153046269787 26.858144805372646 18.982821088899726 30-31 19.022949317738437 26.382096239870616 31.144614622900825 23.450339819490125 32-33 18.71278587733176 30.873172836147422 27.260252889513794 23.153788397007023 34-35 19.07494722620651 30.80407823316733 32.310801571806934 17.810172968819224 36-37 18.771226807616994 26.958773916542363 32.34173715120792 21.928262124632724 38-39 23.997896852484022 31.053087414382674 26.85664748340105 18.092368249732253 40-41 19.363785171866247 34.98620739597197 26.816619076027116 18.833388356134673 42-43 19.48603924217722 30.77308820569465 31.448888596885897 18.291983955242234 44-45 19.14149184449558 26.421779809457156 31.544572008210043 22.892156337837218 46-47 23.97495712436163 26.197412061949997 26.982953468350797 22.844677345337576 48-49 19.881101383535658 31.019509036142388 30.830604183349514 18.26878539697244 50-51 24.17407395744751 26.358705589945096 30.556665944623884 18.910554507983505 52-53 19.304113424872646 26.18792052040668 26.562478730658395 27.945487324062274 54-55 24.030382949681815 26.394477378415566 30.88184791644734 18.69329175545528 56-57 19.617370137826253 26.003655762909073 30.851734672206582 23.527239427058092 58-59 19.508298446190633 25.93650275313989 31.367688232876183 23.187510567793296 60-61 23.776116272799598 26.459601389449645 30.8221091905159 18.942173147234854 62-63 19.229194740166854 26.61698596761341 30.701387012784775 23.452432279434966 64-65 24.06628757420637 26.320984996636593 30.840707836098176 18.77201959305886 66-67 19.954711627207477 34.987423577345794 26.183954947971195 18.873909847475534 68-69 19.89236931911994 35.04596969047556 26.240896627037667 18.82076436336683 70-71 19.487765879813214 35.59026353261355 26.390420811328713 18.531549776244525 72-73 19.44744587642878 35.77050921460545 26.060273433504367 18.721771475461406 74-75 20.376844824986865 34.642042163771706 25.726350555617934 19.254762455623496 76-77 20.78482116488017 33.187287813624685 26.314278074856603 19.713612946638538 78-79 20.436397772634713 32.65765720798139 27.10961800492933 19.79632701445457 80-81 19.873810049512393 32.76776909086735 26.92276732532245 20.435653534297806 82-83 19.05744179964329 32.616610793525645 27.275167551362543 21.050779855468516 84-85 19.28878971013355 31.989208109163986 27.580504719637254 21.141497461065203 86-87 19.217397381371175 31.971940410129623 28.05667645067426 20.753985757824935 88-89 19.476813179342955 32.406339718922275 27.727253296419498 20.389593805315275 90-91 19.962542176356735 31.550206598912204 27.434048317597327 21.053202907133734 92-93 19.586597816222394 32.03723972441791 26.937634970357234 21.438527489002464 94-95 19.288741208536376 32.30493592635184 26.85502264973284 21.551300215378948 96-97 19.921142149077482 32.57946914857446 26.591819993308857 20.9075687090392 98-99 20.281714917799487 31.555966733105812 26.670747176215382 21.491571172879322 100-101 20.17538135329013 31.61430551447354 26.35177578226333 21.858537349972995 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 64.0 1 43.0 2 32.5 3 82.5 4 102.0 5 76.0 6 72.0 7 65.0 8 66.0 9 74.5 10 71.0 11 70.5 12 82.0 13 88.5 14 83.0 15 90.0 16 115.5 17 129.5 18 143.5 19 179.5 20 214.0 21 296.5 22 418.0 23 565.0 24 743.5 25 995.5 26 1418.5 27 1983.0 28 2719.0 29 3647.5 30 5221.5 31 8728.5 32 19362.5 33 41581.5 34 78219.0 35 129476.0 36 202128.0 37 298795.0 38 383476.5 39 412609.5 40 417681.5 41 396204.0 42 363611.5 43 374452.5 44 368712.5 45 341653.0 46 341725.5 47 313606.5 48 242131.5 49 187451.5 50 150759.0 51 120577.5 52 91804.0 53 63522.0 54 44654.5 55 31707.0 56 22479.0 57 15295.0 58 9266.5 59 5848.5 60 3989.0 61 2830.5 62 1876.0 63 1109.0 64 711.5 65 482.0 66 371.5 67 323.0 68 239.0 69 147.5 70 89.0 71 62.5 72 43.0 73 27.0 74 23.5 75 18.0 76 15.0 77 13.5 78 9.0 79 7.0 80 3.5 81 1.5 82 1.0 83 1.0 84 0.5 85 0.5 86 0.5 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18857182163465092 2 0.0 3 0.0 4 0.02718773713269558 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 1.1797082200435331E-4 48-49 0.0033576310878162094 50-51 1.1797082200435331E-4 52-53 0.00264980615579009 54-55 0.009020230544025168 56-57 0.01319458270725613 58-59 0.013857034246203654 60-61 0.0031489134796546616 62-63 0.0021506988319255176 64-65 0.009782503547745606 66-67 0.016652035259845255 68-69 0.02487369408568711 70-71 0.02078101402999762 72-73 0.01619830132905928 74-75 0.020880835494770534 76-77 0.01495507035870571 78-79 0.006678963461169541 80-81 0.0037478422682921475 82-83 6.17078145868925E-4 84-85 0.0026225821199429315 86-87 0.015372505575028807 88-89 0.014365216248683943 90-91 0.017686548622037276 92-93 0.01794063962327742 94-95 0.01709669451201551 96-97 0.019310916094251063 98-99 0.027913711421953133 100-101 0.015799015469967623 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5509837.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.494683367851962 #Duplication Level Percentage of deduplicated Percentage of total 1 79.68970055467508 17.12904881101678 2 7.80910940064309 3.357086679034788 3 3.292114947336743 2.1228890521072783 4 1.7038175126689383 1.4649207180567971 5 1.2296752414211285 1.3215739979817038 6 0.829044244472062 1.0692026119720217 7 0.5485668120784312 0.8253888952216478 8 0.5280016346942117 0.9079382364368255 9 0.3241684642980381 0.6271108648136238 >10 3.1051728667398226 13.43825688302861 >50 0.4171586731480179 6.256672040574045 >100 0.41683901713821125 19.51332113979308 >500 0.07029908154057977 10.580431573342478 >1k 0.035736933276119466 12.491083071268429 >5k 5.096713975636169E-4 0.7395492714376847 >10k+ 8.49444718499034E-5 8.155526153914073 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT 446669 8.106755245209614 TruSeq Adapter, Index 13 (97% over 38bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 8783 0.15940580456372846 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 7402 0.13434154222711125 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 7276 0.13205472321594994 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 6554 0.11895088729485101 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.005027371953108595 0.0 0.0 2 0.0055537033128203244 0.0 0.0 3 0.005971138529143421 0.0 0.0 4 0.006424872459929396 0.0 0.0 5 0.007368639035964222 0.0 0.0 6 0.008185360111378975 0.0 0.0 7 0.00932876961695963 0.0 0.0 8 0.010454029765308846 0.0 0.0 9 0.011561140556426625 0.0 0.0 10-11 0.017241889369867022 0.0 0.0 12-13 0.022441680216674288 0.0 0.0 14-15 0.02751442556286148 0.0 0.0 16-17 0.03187934597702255 0.0 0.0 18-19 0.037433049289842875 0.0 0.0 20-21 0.04352215864099065 0.0 0.0 22-23 0.05050966117509465 0.0 0.0 24-25 0.058177764605377616 0.0 0.0 26-27 0.06638127407398803 0.0 0.0 28-29 0.0750748161878473 0.0 0.0 30-31 0.11660054553337966 0.0 0.0 32-33 0.12735403969300724 0.0 0.0 34-35 0.13712746856213714 0.0 0.0 36-37 0.1480533816154634 0.0 0.0 38-39 0.16028604838945326 0.0 0.0 40-41 0.17415215731427264 0.0 0.0 42-43 0.1872196945209087 0.0 0.0 44-45 0.20179362837775416 0.0 0.0 46-47 0.21747467302571744 0.0 0.0 48-49 0.23429912717926138 0.0 0.0 50-51 0.25288406898425486 0.0 0.0 52-53 0.2732476477979294 0.0 0.0 54-55 0.29374734679083975 0.0 0.0 56-57 0.31582603986288527 0.0 0.0 58-59 0.33981949012284757 0.0 0.0 60-61 0.3656369507845695 0.0 0.0 62-63 0.39288821066757507 0.0 0.0 64-65 0.42131917877062425 0.0 0.0 66-67 0.45296258310363813 0.0 0.0 68-69 0.487192270842132 0.0 0.0 70-71 0.5234002385188528 0.0 0.0 72-73 0.5617498303488833 0.0 1.814935723143897E-5 74-75 0.6034116798736515 0.0 1.814935723143897E-5 76-77 0.6503912910672311 0.0 1.814935723143897E-5 78-79 0.7007376080272429 0.0 1.814935723143897E-5 80-81 0.7555214428303414 0.0 1.814935723143897E-5 82-83 0.8141892400809678 0.0 1.814935723143897E-5 84-85 0.8776212436048472 0.0 1.814935723143897E-5 86-87 0.9476051650892758 0.0 1.814935723143897E-5 88-89 1.0231790886009877 0.0 1.814935723143897E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 57155 0.0 82.54639 3 GATCGGA 57435 0.0 82.40186 1 GAAGAGC 57020 0.0 82.37623 6 CGGAAGA 57060 0.0 82.3425 4 ATCGGAA 58010 0.0 81.35432 2 GGAAGAG 58330 0.0 80.64018 5 CGTCCGT 25 5.2757838E-5 76.00385 3 CGCGCTG 20 0.0020599284 71.37669 1 AAGAGCA 81885 0.0 57.315662 7 GAGCACA 81945 0.0 57.273697 9 AGAGCAC 82250 0.0 57.10751 8 GTATCGC 25 0.00498709 57.10136 1 CGAGCGT 25 0.00498709 57.10136 1 GCGTCCG 35 2.7881772E-4 54.28846 2 GCGATCC 45 1.516273E-5 52.78045 2 ATCGGAC 165 0.0 51.82081 2 TCGGACG 175 0.0 51.57404 3 GGCCCGC 65 4.3619366E-8 51.244812 1 CGGCAGA 150 0.0 50.66923 4 GCCGTGG 110 0.0 47.58447 1 >>END_MODULE