FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n01_34042-1.3410000003584a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n01_34042-1.3410000003584a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5509837
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT4466698.106755245209614TruSeq Adapter, Index 13 (97% over 38bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC87830.15940580456372846No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG74020.13434154222711125No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC72760.13205472321594994No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG65540.11895088729485101No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG571550.082.546393
GATCGGA574350.082.401861
GAAGAGC570200.082.376236
CGGAAGA570600.082.34254
ATCGGAA580100.081.354322
GGAAGAG583300.080.640185
CGTCCGT255.2757838E-576.003853
CGCGCTG200.002059928471.376691
AAGAGCA818850.057.3156627
GAGCACA819450.057.2736979
AGAGCAC822500.057.107518
GTATCGC250.0049870957.101361
CGAGCGT250.0049870957.101361
GCGTCCG352.7881772E-454.288462
GCGATCC451.516273E-552.780452
ATCGGAC1650.051.820812
TCGGACG1750.051.574043
GGCCCGC654.3619366E-851.2448121
CGGCAGA1500.050.669234
GCCGTGG1100.047.584471
Produced by FastQC (version 0.11.2)