##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n01_34012-1.3410000003579b.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2911263 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.674057616917466 33.0 33.0 33.0 33.0 33.0 2 32.69603742430691 33.0 33.0 33.0 33.0 33.0 3 32.734825744015566 33.0 33.0 33.0 33.0 33.0 4 36.65242130305644 37.0 37.0 37.0 37.0 37.0 5 36.65120808391409 37.0 37.0 37.0 37.0 37.0 6 36.528817561312735 37.0 37.0 37.0 37.0 37.0 7 36.38440429463089 37.0 37.0 37.0 37.0 37.0 8 36.3345262863575 37.0 37.0 37.0 37.0 37.0 9 36.40588844085883 37.0 37.0 37.0 37.0 37.0 10-11 36.33028379778811 37.0 37.0 37.0 35.0 37.0 12-13 36.323593059094975 37.0 37.0 37.0 35.0 37.0 14-15 38.16049820988348 40.0 37.0 40.0 37.0 40.0 16-17 38.186123342343166 40.0 37.0 40.0 37.0 40.0 18-19 38.13166278690726 40.0 37.0 40.0 37.0 40.0 20-21 38.14219876390419 40.0 37.0 40.0 37.0 40.0 22-23 37.961149679709465 40.0 37.0 40.0 33.0 40.0 24-25 38.00686488991204 40.0 37.0 40.0 35.0 40.0 26-27 37.876493638671604 40.0 37.0 40.0 33.0 40.0 28-29 37.69353266949774 40.0 37.0 40.0 33.0 40.0 30-31 37.67326569258772 40.0 37.0 40.0 33.0 40.0 32-33 37.56045640672107 40.0 37.0 40.0 33.0 40.0 34-35 37.51932752210982 40.0 37.0 40.0 33.0 40.0 36-37 37.49115023273404 40.0 37.0 40.0 33.0 40.0 38-39 37.329808917985076 40.0 37.0 40.0 33.0 40.0 40-41 37.2500124516404 40.0 37.0 40.0 33.0 40.0 42-43 36.9880086409232 38.5 37.0 40.0 33.0 40.0 44-45 36.94641380734066 37.0 37.0 40.0 33.0 40.0 46-47 36.984784782412305 37.0 37.0 40.0 33.0 40.0 48-49 36.6007466518827 37.0 37.0 40.0 33.0 40.0 50-51 36.568289948383224 37.0 37.0 40.0 33.0 40.0 52-53 36.51827797763376 37.0 37.0 40.0 33.0 40.0 54-55 36.192288020697546 37.0 37.0 40.0 33.0 40.0 56-57 36.09725023125702 37.0 37.0 40.0 33.0 40.0 58-59 35.77450044877429 37.0 37.0 40.0 27.0 40.0 60-61 35.496201648562845 37.0 35.0 40.0 27.0 40.0 62-63 35.27672989352044 37.0 33.0 38.5 27.0 40.0 64-65 35.03570924371999 37.0 33.0 37.0 27.0 40.0 66-67 34.33397669671204 37.0 33.0 37.0 27.0 40.0 68-69 34.11537981968651 37.0 33.0 37.0 27.0 40.0 70-71 33.62083346643708 37.0 33.0 37.0 27.0 40.0 72-73 32.54007916838843 37.0 33.0 37.0 22.0 38.5 74-75 31.60955897835407 37.0 30.0 37.0 15.0 37.0 76-77 29.22969841611699 33.0 27.0 35.0 15.0 37.0 78-79 31.140134367798446 33.0 33.0 37.0 15.0 37.0 80-81 31.35727929767939 37.0 33.0 37.0 15.0 37.0 82-83 31.21533489073299 37.0 33.0 37.0 15.0 37.0 84-85 31.000257620146307 33.0 33.0 37.0 15.0 37.0 86-87 30.869513506680775 33.0 33.0 37.0 15.0 37.0 88-89 30.72371407186503 33.0 33.0 37.0 15.0 37.0 90-91 30.56200023838451 33.0 33.0 37.0 15.0 37.0 92-93 30.430335218769308 33.0 27.0 37.0 15.0 37.0 94-95 30.301434978564288 33.0 27.0 37.0 15.0 37.0 96-97 30.131518176131802 33.0 27.0 37.0 15.0 37.0 98-99 29.965091611441494 33.0 27.0 37.0 10.5 37.0 100-101 28.439545310746574 33.0 24.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.11503529326221695 2306 2 0.16073055378529233 2306 3 0.1447133591506713 2306 4 0.15664276458431914 2306 5 0.20626727553076307 2306 6 0.2120883009236323 2306 7 0.19718549920746398 2306 8 0.26335729645833794 2306 9 0.2442804791861235 2306 10-11 0.28198023943820516 2306 12-13 0.24218487503067365 2306 14-15 0.5135591091803562 2306 16-17 0.5439775048134123 2306 18-19 0.3844610945324973 2306 20-21 0.5465574831095452 2306 22-23 0.49555085813226896 2306 24-25 0.4959598216384009 2306 26-27 0.6089750002495506 2306 28-29 0.5660817948689711 2306 30-31 0.4660371464197155 2306 32-33 0.5547223927481326 2306 34-35 0.5247088011073018 2306 36-37 0.556935792282772 2306 38-39 0.48307651017113784 2306 40-41 0.4713330026269418 2306 42-43 0.4599917226199395 2306 44-45 0.46900968918610175 2306 46-47 0.4198210044590027 2306 48-49 0.5181679719646937 2306 50-51 0.6130262569911267 2306 52-53 0.5926024575834248 2306 54-55 0.4446893892821464 2306 56-57 0.5007915787804507 2306 58-59 0.5881497868733234 2306 60-61 0.5621620023095488 2306 62-63 0.556088182907196 2306 64-65 0.5681847043697559 2306 66-67 0.5988139943619828 2306 68-69 0.743863324978129 2306 70-71 0.7202215537298926 2306 72-73 0.38843522488137694 2306 74-75 0.25465067563588306 2306 76-77 0.5054739069150251 2306 78-79 0.6026989024838052 2306 80-81 0.48231732709943387 2306 82-83 0.34572277525714057 2306 84-85 0.4150277619064795 2306 86-87 0.41599782400693996 2306 88-89 0.2664229141504961 2306 90-91 0.20697336119210163 2306 92-93 0.26230440794684284 2306 94-95 0.24962213729525118 2306 96-97 0.2539475316411277 2306 98-99 0.5185334001529007 2306 100-101 0.24733844384288162 2307 1 0.1379733718253675 2307 2 0.09526905087640358 2307 3 0.11271277706477889 2307 4 0.1829575562272865 2307 5 0.21176552773587787 2307 6 0.18081348157009103 2307 7 0.24203890681823736 2307 8 0.21688204177013404 2307 9 0.23346044518329023 2307 10-11 0.21147692984946076 2307 12-13 0.18661770508516184 2307 14-15 0.451204489744633 2307 16-17 0.5049257924253325 2307 18-19 0.508110076240861 2307 20-21 0.45523712168338903 2307 22-23 0.5154771545800116 2307 24-25 0.48769235726810223 2307 26-27 0.48093034770256793 2307 28-29 0.47530385961373156 2307 30-31 0.6700617374992461 2307 32-33 0.6689208819892798 2307 34-35 0.6182942760145878 2307 36-37 0.5608653844844653 2307 38-39 0.5627836430804152 2307 40-41 0.6265816650078975 2307 42-43 0.6465205333098254 2307 44-45 0.6810902260988527 2307 46-47 0.6483126743794045 2307 48-49 0.7414480259921064 2307 50-51 0.7526314633805455 2307 52-53 0.7024065057448752 2307 54-55 0.724621977996442 2307 56-57 0.7479187081849048 2307 58-59 0.7532083387706905 2307 60-61 0.7478108180004455 2307 62-63 0.6597643422880495 2307 64-65 0.5821169508011508 2307 66-67 0.7063721952656508 2307 68-69 0.8904389074995649 2307 70-71 0.5744195920594279 2307 72-73 0.2473786979870667 2307 74-75 0.17359808397016607 2307 76-77 0.40969997350032017 2307 78-79 0.47798054290024083 2307 80-81 0.5378257760557261 2307 82-83 0.5205268592865728 2307 84-85 0.4472958165610663 2307 86-87 0.5027415350709781 2307 88-89 0.4673329357635083 2307 90-91 0.5129887270300095 2307 92-93 0.6238014754841785 2307 94-95 0.40367967566629304 2307 96-97 0.5019825305804595 2307 98-99 0.49789261376305305 2307 100-101 0.48488236929489403 2201 1 0.09485565100737148 2201 2 0.07611346229143834 2201 3 0.10770442247434175 2201 4 0.14785297320599966 2201 5 0.11557286810676715 2201 6 0.15706578541894345 2201 7 0.16885805073813032 2201 8 0.1976811174568951 2201 9 0.18419141206812384 2201 10-11 0.18526168843671087 2201 12-13 0.26523837033437303 2201 14-15 0.41647158317928046 2201 16-17 0.35379292526570794 2201 18-19 0.29192856881832796 2201 20-21 0.32047666935907415 2201 22-23 0.42227416624774605 2201 24-25 0.4074889586187993 2201 26-27 0.46145922627454894 2201 28-29 0.48178677311334894 2201 30-31 0.5474757454741308 2201 32-33 0.5186817216758328 2201 34-35 0.4851579713737948 2201 36-37 0.57760612820082 2201 38-39 0.5170381716318744 2201 40-41 0.4548483003030057 2201 42-43 0.3792630675005171 2201 44-45 0.47691905216172614 2201 46-47 0.5384771168216886 2201 48-49 0.5380879216242818 2201 50-51 0.5882519555123125 2201 52-53 0.508265622578115 2201 54-55 0.5994341551348086 2201 56-57 0.47620303780434625 2201 58-59 0.44715687984973584 2201 60-61 0.4423566620818917 2201 62-63 0.5687639625774139 2201 64-65 0.5955107891591922 2201 66-67 0.506329059372284 2201 68-69 0.5272277032621062 2201 70-71 0.4303104152089645 2201 72-73 0.06659511678972763 2201 74-75 0.10374959520893157 2201 76-77 0.05371494002935506 2201 78-79 0.22508599055229794 2201 80-81 0.36546574533455356 2201 82-83 0.27755748385796153 2201 84-85 0.29418864538617484 2201 86-87 0.34849743182411785 2201 88-89 0.2865604493857461 2201 90-91 0.23276772970137571 2201 92-93 0.2628763779501959 2201 94-95 0.13972722270689175 2201 96-97 0.2774937769973569 2201 98-99 0.19856911082244721 2201 100-101 0.211097323772659 1101 1 0.08860170336346584 1101 2 0.06928788701306132 1101 3 0.059949499245568916 1101 4 0.06687503074878975 1101 5 0.12061740720669434 1101 6 0.19582439106273597 1101 7 0.1849118319432108 1101 8 0.19622029127990004 1101 9 0.25740056725913263 1101 10-11 0.21285235449111184 1101 12-13 0.19741534090144341 1101 14-15 0.3498359698451665 1101 16-17 0.3261965411755767 1101 18-19 0.2796003957923219 1101 20-21 0.27089707694066334 1101 22-23 0.35251281066861395 1101 24-25 0.3288736205597331 1101 26-27 0.3420083160512277 1101 28-29 0.36599074336549364 1101 30-31 0.46407256413498743 1101 32-33 0.4356317390058848 1101 34-35 0.38583094019554665 1101 36-37 0.522405970558971 1101 38-39 0.465511016240292 1101 40-41 0.5007192627099926 1101 42-43 0.5231548857831001 1101 44-45 0.4858821171696448 1101 46-47 0.5481027702172341 1101 48-49 0.6248642162315647 1101 50-51 0.6082767805844043 1101 52-53 0.501278270873172 1101 54-55 0.6330471444007344 1101 56-57 0.7808073761220484 1101 58-59 0.6652805899928254 1101 60-61 0.5903592202431085 1101 62-63 0.7026013627537111 1101 64-65 0.6663465196690481 1101 66-67 0.7832233566432905 1101 68-69 0.7374160113745063 1101 70-71 0.6337009464936543 1101 72-73 0.4264531863456682 1101 74-75 0.47626607634733276 1101 76-77 0.2098422111891658 1101 78-79 0.6629185383154912 1101 80-81 0.76906282966414 1101 82-83 0.6443910291984665 1101 84-85 0.7453017270203688 1101 86-87 0.7584434711606853 1101 88-89 0.667723744170722 1101 90-91 0.6082534548450731 1101 92-93 0.8416226150428265 1101 94-95 0.6848642532341671 1101 96-97 0.6583514647644577 1101 98-99 0.705775067432235 1101 100-101 0.6565259741226903 2304 1 0.06553689384129058 2304 2 0.068637300182111 2304 3 0.08347224372860751 2304 4 0.09700884481612349 2304 5 0.08185155169722691 2304 6 0.0534224147118465 2304 7 0.10377201032940064 2304 8 0.11279844882450618 2304 9 0.0852984471114624 2304 10-11 0.1404642692682998 2304 12-13 0.0953532493502749 2304 14-15 0.21123402313303785 2304 16-17 0.3402659088768232 2304 18-19 0.2542236824039179 2304 20-21 0.21420270944296504 2304 22-23 0.28447763175316254 2304 24-25 0.25753209260078336 2304 26-27 0.2549077674086675 2304 28-29 0.28918225039818424 2304 30-31 0.2043424233183515 2304 32-33 0.13145039545857884 2304 34-35 0.30222133137818474 2304 36-37 0.3329793500766485 2304 38-39 0.2606177727351735 2304 40-41 0.2715018071152002 2304 42-43 0.30528418564093585 2304 44-45 0.2867777936578051 2304 46-47 0.24094924638165338 2304 48-49 0.1674529004176648 2304 50-51 0.21940100114229466 2304 52-53 0.21182062741429775 2304 54-55 0.22945523740778384 2304 56-57 0.2072938326792979 2304 58-59 0.13303135091071994 2304 60-61 0.34446874682109296 2304 62-63 0.2076085200231006 2304 64-65 0.012415232712214674 2304 66-67 0.26105446788468356 2304 68-69 0.37115907598860787 2304 70-71 0.29304749349794434 2304 72-73 0.04581323363738932 2304 74-75 0.1990779793993056 2304 76-77 0.1554934575637148 2304 78-79 0.21252460351096758 2304 80-81 0.20169509258264995 2304 82-83 0.20964146848707088 2304 84-85 0.27366959275358127 2304 86-87 0.21396466530915248 2304 88-89 0.21547820178975385 2304 90-91 0.12005708673180848 2304 92-93 0.2359993137747125 2304 94-95 0.07633668024060469 2304 96-97 0.33550691023323154 2304 98-99 0.49917735157012544 2304 100-101 0.26985885147526645 2202 1 0.07090763462655048 2202 2 0.09453402811055156 2202 3 0.06615183354801957 2202 4 0.10163675493031121 2202 5 0.0727459483378965 2202 6 0.07164427617347968 2202 7 0.05641002714258292 2202 8 0.03885993509512531 2202 9 0.17861013480924015 2202 10-11 0.13555070699605665 2202 12-13 0.197258737578359 2202 14-15 0.2245680587790062 2202 16-17 0.06405873025126141 2202 18-19 0.11774910574612107 2202 20-21 0.09793537804039687 2202 22-23 0.10782889656869088 2202 24-25 0.07469940462973312 2202 26-27 0.04587424113444882 2202 28-29 0.16545562349021026 2202 30-31 0.06422354940162478 2202 32-33 0.0386935797620751 2202 34-35 0.058577748437237176 2202 36-37 0.19188166544233098 2202 38-39 0.1549722446283326 2202 40-41 0.3005479119887795 2202 42-43 0.14300067013400763 2202 44-45 0.22137980674393276 2202 46-47 0.3050796940504341 2202 48-49 0.3729639086429728 2202 50-51 0.14517968652409508 2202 52-53 0.14073458346182832 2202 54-55 0.1748883219453603 2202 56-57 0.3085015853103812 2202 58-59 0.036388330270078484 2202 60-61 -0.12010370760700084 2202 62-63 0.1250342620921785 2202 64-65 0.1365455393181989 2202 66-67 0.14098189257777705 2202 68-69 0.30403352004737627 2202 70-71 0.25348493222379176 2202 72-73 0.015403171598272536 2202 74-75 0.032360965320489044 2202 76-77 0.046313931297596866 2202 78-79 0.20818392125626062 2202 80-81 0.19219939057089874 2202 82-83 0.1472597492647587 2202 84-85 0.11261587469098444 2202 86-87 0.0872149426701192 2202 88-89 -0.05292752902131781 2202 90-91 -0.024074948643740157 2202 92-93 0.04721047442287585 2202 94-95 -0.26136322187081973 2202 96-97 -0.009954972123701111 2202 98-99 0.10557141288652261 2202 100-101 0.11829790912177884 1102 1 0.09346781509352553 1102 2 0.0957621072184267 1102 3 0.0732571086402416 1102 4 0.11703671113790648 1102 5 0.1261746233451504 1102 6 0.0709878075157846 1102 7 0.11994016752588266 1102 8 0.05819898147250058 1102 9 0.1436240696398059 1102 10-11 0.09999219670554282 1102 12-13 0.061516557957737916 1102 14-15 0.14750164318133585 1102 16-17 0.16417437390354905 1102 18-19 0.09793972232029091 1102 20-21 0.056248018491167784 1102 22-23 0.1149294418591893 1102 24-25 0.02977637380617182 1102 26-27 0.057807252674329845 1102 28-29 0.16793181380742084 1102 30-31 0.1746145481994219 1102 32-33 0.23447899255273796 1102 34-35 0.14690672617962264 1102 36-37 0.1174109372114529 1102 38-39 0.17198019431214817 1102 40-41 0.15244898623804204 1102 42-43 0.2092530896362632 1102 44-45 0.1430742526490505 1102 46-47 0.30833498009075555 1102 48-49 0.1247999063849079 1102 50-51 0.08834284159119932 1102 52-53 -0.01812443255577989 1102 54-55 -0.004044389947672755 1102 56-57 0.12211769679570494 1102 58-59 0.1859571114641696 1102 60-61 0.01865525084614461 1102 62-63 0.06270117888189475 1102 64-65 0.036359713754954726 1102 66-67 0.011747641913309792 1102 68-69 -0.013065255740819737 1102 70-71 -0.01623006383517378 1102 72-73 -0.1312146306520532 1102 74-75 -0.17138797290857966 1102 76-77 -0.10436739664153905 1102 78-79 -0.18990596222646516 1102 80-81 -0.0184492850428839 1102 82-83 -0.07737453272768846 1102 84-85 -0.09900609674045668 1102 86-87 -0.19744254039093434 1102 88-89 -0.225475565026791 1102 90-91 -0.15851511122649953 1102 92-93 -0.16662323576436577 1102 94-95 -0.3786053384737862 1102 96-97 -0.2760872129284806 1102 98-99 -0.0849096663763298 1102 100-101 -0.28390982321373315 2305 1 -0.04828285944572741 2305 2 -0.04202188525097483 2305 3 0.024558839618826767 2305 4 0.006859281294211428 2305 5 0.04278211092904627 2305 6 0.06626648026087167 2305 7 0.07035018459042419 2305 8 0.10592958618341441 2305 9 0.004774725817988212 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-0.1559531007628543 1213 96-97 -0.12024990551835302 1213 98-99 0.10953732555323725 1213 100-101 0.1133067945412165 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 7.0 9 15.0 10 32.0 11 21.0 12 36.0 13 82.0 14 280.0 15 623.0 16 1181.0 17 2006.0 18 3010.0 19 4364.0 20 6031.0 21 8481.0 22 11399.0 23 15075.0 24 19643.0 25 26652.0 26 36251.0 27 54689.0 28 97299.0 29 147769.0 30 102244.0 31 66707.0 32 83389.0 33 116977.0 34 178915.0 35 302702.0 36 570235.0 37 828260.0 38 226725.0 39 162.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.23986415679786 22.834233230306054 21.359134089065538 13.56676852383055 2 27.579370190875917 30.32886413903519 29.700374030103088 12.391391639985807 3 33.950969046767675 17.279819789555255 33.6021170193143 15.167094144362773 4 26.84056220221867 17.49984452752344 30.05710735984371 25.60248591041418 5 38.41229734311191 16.172396653960842 30.108993931499832 15.306312071427419 6 33.33268069562935 17.051327894456804 34.158507836633106 15.457483573280737 7 29.33833185115876 27.77145177196289 26.102176271947947 16.788040104930403 8 26.429113412288757 34.7497288977327 22.72501659932476 16.09614109065378 9 36.49076019583253 22.94540204715273 23.359277399534154 17.204560357480585 10-11 26.53662688668114 30.84798590852149 26.872546382789874 15.742840822007492 12-13 21.140601175503555 29.26552839781222 26.79222042117115 22.80165000551307 14-15 20.43554635908882 29.871983396896812 27.435755546647624 22.256714697366746 16-17 25.05805899363953 25.081674173717726 28.185121028227268 21.67514580441547 18-19 19.127231033403717 25.181098375516054 33.63426801357349 22.05740257750674 20-21 24.37387140907572 25.714560999813486 33.20600028235168 16.70556730875912 22-23 18.800122146298705 36.47552625784754 27.780107808878828 16.94424378697493 24-25 19.22220699400913 25.260170585756082 33.1236133595625 22.394009060672293 26-27 19.244911916237044 25.509667110116812 27.663388707925048 27.582032265721097 28-29 24.310960569347394 31.074073348921072 27.530594109841672 17.084371971889865 30-31 18.57020475305735 26.357907203849322 32.53874692873849 22.533141114354834 32-33 18.179206069668044 31.84964738671841 27.54306292492296 22.428083618690582 34-35 23.726746776227362 26.33607819011886 27.962228077641903 21.974946956011877 36-37 18.2681880682027 26.35217086192488 33.51596884238903 21.8636722274834 38-39 18.829851511182603 31.572413759938556 27.475034031621327 22.122700697257514 40-41 18.853483865937225 25.940201898626132 37.83694568302486 17.36936855241179 42-43 18.51692890680093 31.30938359055846 32.65287265355277 17.520814849087834 44-45 18.29142540539965 26.056560331375078 32.928199891249946 22.723814371975326 46-47 23.782472843798363 26.099631448175607 27.461739497973298 22.65615621005273 48-49 18.800737988366826 31.737604670582865 32.1264363275495 17.335221013500817 50-51 24.232213623040753 26.31349529533442 31.80476213212222 17.649528949502606 52-53 18.45832723615356 26.150065506038437 26.793670062538173 28.597937195269825 54-55 23.938724373732814 26.294032452323584 32.02078476718134 17.746458406762265 56-57 18.709836557516635 26.20569557143375 31.967381929720982 23.11708594132863 58-59 18.576520133288057 26.15749722975012 32.55369543861674 22.71228719834508 60-61 23.574116732126996 26.392271444755355 32.404974893324976 17.62863692979268 62-63 18.255079180461102 26.509722114401015 32.04716752347315 23.18803118166473 64-65 23.585403775783483 26.364509861310932 31.985810925010615 18.06427543789497 66-67 18.47735698768137 36.73152442090187 26.585941892385634 18.205176699031128 68-69 18.40517103650586 37.10479127605764 26.410083562416187 18.079954125020315 70-71 18.01435145325127 37.39173365637731 26.65016632046632 17.9437485699051 72-73 18.030275285776174 36.81978605565494 26.60660096325458 18.543337695314314 74-75 18.536284868418115 36.04978112918171 26.416939890147155 18.99699411225302 76-77 18.820004211890378 34.97751654438099 26.81869667071709 19.38378257301154 78-79 19.055182945523665 34.33742524015641 27.04146909479894 19.565922719520973 80-81 18.750933906392607 34.26061578910667 26.965789226902416 20.022661077598304 82-83 18.464433865161837 33.747686342494994 27.15210723382827 20.635772558514905 84-85 18.698614350042266 32.96168208472323 27.604521439119374 20.735182126115134 86-87 18.73033933874045 32.81684186803358 27.8957954248558 20.55702336837017 88-89 19.094812563438065 33.181039033273024 27.43104457296531 20.293103830323606 90-91 19.557247992897995 32.6223539037419 27.06271097610854 20.757687127251565 92-93 19.18772186439528 32.69662564277868 27.296090892147166 20.819561600678874 94-95 18.869746987585486 32.663201690700305 27.315724677501112 21.151326644213093 96-97 19.113483376524947 33.32540275049791 26.893711523073872 20.66740234990327 98-99 19.42473468706181 32.55019617369386 26.899950557151158 21.125118582093165 100-101 19.6106481204733 32.46267503004343 26.409000867806842 21.517675981676426 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 190.0 1 120.0 2 96.0 3 210.0 4 217.0 5 132.5 6 104.5 7 105.0 8 110.0 9 108.0 10 113.5 11 128.0 12 132.0 13 135.5 14 146.0 15 148.0 16 170.5 17 202.0 18 249.0 19 352.5 20 486.0 21 734.5 22 1203.5 23 1911.5 24 2949.5 25 4632.0 26 7477.5 27 11548.5 28 16085.0 29 20634.5 30 25611.0 31 30330.5 32 36794.0 33 48264.5 34 65139.0 35 89707.5 36 118594.0 37 151978.5 38 189891.0 39 211950.0 40 215149.0 41 205669.0 42 191639.5 43 184656.0 44 171196.5 45 155059.0 46 148674.0 47 133064.5 48 103593.0 49 79249.0 50 63729.5 51 50933.5 52 40216.5 53 32218.0 54 26098.5 55 20138.5 56 14484.0 57 10315.5 58 7222.5 59 5399.0 60 4012.0 61 2848.0 62 2026.5 63 1388.5 64 965.0 65 673.5 66 476.5 67 343.5 68 236.5 69 170.5 70 127.0 71 84.0 72 60.5 73 42.0 74 26.0 75 21.0 76 16.0 77 12.5 78 10.5 79 8.0 80 5.0 81 2.5 82 1.5 83 1.0 84 0.5 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19098240179605896 2 0.0 3 0.0 4 0.026792495215993885 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 1.202227349435623E-4 48-49 0.0036410314011478865 50-51 1.3739741136407118E-4 52-53 0.0027822975801224414 54-55 0.009789565559690072 56-57 0.01451260157533002 58-59 0.014581300281012056 60-61 0.002919694991486513 62-63 0.002232707934666157 64-65 0.00999566167673618 66-67 0.01746664591965755 68-69 0.025555918513717246 70-71 0.02119355070290798 72-73 0.017380772537555007 74-75 0.022052284523933426 76-77 0.015663304895504115 78-79 0.006577901069054908 80-81 0.0035723326954658513 82-83 6.011136747178115E-4 84-85 0.0022670572875071746 86-87 0.016127021158857856 88-89 0.015508732807719536 90-91 0.01842842779920605 92-93 0.019252812267390475 94-95 0.017741440742385692 96-97 0.0202661181762005 98-99 0.02857866156372681 100-101 0.016384641305165492 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2911263.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.4853231720533 #Duplication Level Percentage of deduplicated Percentage of total 1 90.29773092791787 45.58710127599046 2 4.7813138780133375 4.827723526370535 3 1.2934673314890388 1.9590334872815256 4 0.8131647879268212 1.6421154844247905 5 0.46719070546325686 1.1793136874146044 6 0.30102896627573456 0.9118526787947756 7 0.26138818496668764 0.9237386893979769 8 0.21879613641432757 0.8836794924539191 9 0.12834310851640232 0.583149897932083 >10 1.2088756306205042 11.68413915217555 >50 0.12252422430091188 4.262685564741039 >100 0.09194343724908723 9.957769182002776 >500 0.011646649617926682 3.8994095444228454 >1k 0.0025186699216695847 2.0916250151772986 >5k 0.0 0.0 >10k+ 6.736130633173584E-5 9.60666332141973 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT 282486 9.703211286647754 TruSeq Adapter, Index 2 (97% over 37bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 3296 0.11321546696399466 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0046715119863784205 0.0 0.0 2 0.00515240292615267 0.0 3.4349352841017796E-5 3 0.006182883511383204 0.0 3.4349352841017796E-5 4 0.006595075745475417 0.0 3.4349352841017796E-5 5 0.0075225082721828975 0.0 3.4349352841017796E-5 6 0.008518639504572415 0.0 3.4349352841017796E-5 7 0.009961312323895162 0.0 3.4349352841017796E-5 8 0.01075134743923857 0.0 3.4349352841017796E-5 9 0.011987924141515212 0.0 3.4349352841017796E-5 10-11 0.018187982329318925 0.0 3.4349352841017796E-5 12-13 0.023615180078199738 0.0 3.4349352841017796E-5 14-15 0.029248473944126656 0.0 3.4349352841017796E-5 16-17 0.03462414766374594 0.0 3.4349352841017796E-5 18-19 0.04063528441092405 0.0 3.4349352841017796E-5 20-21 0.0463201023061125 0.0 3.4349352841017796E-5 22-23 0.05234841372971113 0.0 3.4349352841017796E-5 24-25 0.0590465375337096 0.0 3.4349352841017796E-5 26-27 0.06572748666128755 0.0 3.4349352841017796E-5 28-29 0.07402285537239336 0.0 3.4349352841017796E-5 30-31 0.1397675167101014 0.0 3.4349352841017796E-5 32-33 0.15019254529735032 0.0 3.4349352841017796E-5 34-35 0.15896880494823037 0.0 3.4349352841017796E-5 36-37 0.16855227439087433 0.0 3.4349352841017796E-5 38-39 0.17980168744630767 0.0 3.4349352841017796E-5 40-41 0.19017519200429506 0.0 3.4349352841017796E-5 42-43 0.20200854405802568 0.0 3.4349352841017796E-5 44-45 0.21420256431658702 0.0 3.4349352841017796E-5 46-47 0.22739271580753784 0.0 3.4349352841017796E-5 48-49 0.2414244264430936 1.7174676420508898E-5 3.4349352841017796E-5 50-51 0.2568472858687106 3.4349352841017796E-5 3.4349352841017796E-5 52-53 0.27417653437700407 3.4349352841017796E-5 3.4349352841017796E-5 54-55 0.2929484557046203 3.4349352841017796E-5 3.4349352841017796E-5 56-57 0.3133004472629234 3.4349352841017796E-5 3.4349352841017796E-5 58-59 0.33591949610873356 3.4349352841017796E-5 3.4349352841017796E-5 60-61 0.3592942307170462 3.4349352841017796E-5 3.4349352841017796E-5 62-63 0.3840429394389995 3.4349352841017796E-5 3.4349352841017796E-5 64-65 0.41038889306806015 3.4349352841017796E-5 3.4349352841017796E-5 66-67 0.43980911377639187 3.4349352841017796E-5 3.4349352841017796E-5 68-69 0.47013959233501057 3.4349352841017796E-5 3.4349352841017796E-5 70-71 0.5052274562621103 3.4349352841017796E-5 3.4349352841017796E-5 72-73 0.5420843118605223 3.4349352841017796E-5 3.4349352841017796E-5 74-75 0.5822558800080927 3.4349352841017796E-5 3.4349352841017796E-5 76-77 0.6248834268837958 3.4349352841017796E-5 3.4349352841017796E-5 78-79 0.6726290273328106 3.4349352841017796E-5 3.4349352841017796E-5 80-81 0.7268151314395161 3.4349352841017796E-5 3.4349352841017796E-5 82-83 0.7856212235033386 3.4349352841017796E-5 3.4349352841017796E-5 84-85 0.8493220983470061 3.4349352841017796E-5 3.4349352841017796E-5 86-87 0.9200302411702412 3.4349352841017796E-5 3.4349352841017796E-5 88-89 0.9950148784221831 3.4349352841017796E-5 3.4349352841017796E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCGGA 35370 0.0 84.17756 1 TCGGAAG 35405 0.0 83.82169 3 CGGAAGA 35675 0.0 83.05414 4 ATCGGAA 35775 0.0 82.95478 2 GAAGAGC 36225 0.0 81.90177 6 GGAAGAG 37585 0.0 78.9761 5 CGCGTGG 20 0.002073338 71.25918 2 AGAGCAC 43085 0.0 68.79522 8 GAGCACA 43205 0.0 68.56017 9 AAGAGCA 44145 0.0 67.07877 7 TCGGACG 165 0.0 51.824852 3 CGAGGCG 85 1.2551027E-10 50.415787 1 ATCGGAC 185 0.0 46.222168 2 ATGCCGT 32445 0.0 45.49966 48-49 GCCGTCT 32675 0.0 45.487 50-51 TCGTATG 32690 0.0 45.390354 44-45 TTGTGTT 26430 0.0 45.326942 1 CGTCTTC 32855 0.0 45.24619 52-53 TCTCGTA 32550 0.0 45.24267 42-43 GTATGCC 32795 0.0 45.085716 46-47 >>END_MODULE