FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n01_34012-1.3410000003579b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n01_34012-1.3410000003579b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2911263
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT2824869.703211286647754TruSeq Adapter, Index 2 (97% over 37bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC32960.11321546696399466No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA353700.084.177561
TCGGAAG354050.083.821693
CGGAAGA356750.083.054144
ATCGGAA357750.082.954782
GAAGAGC362250.081.901776
GGAAGAG375850.078.97615
CGCGTGG200.00207333871.259182
AGAGCAC430850.068.795228
GAGCACA432050.068.560179
AAGAGCA441450.067.078777
TCGGACG1650.051.8248523
CGAGGCG851.2551027E-1050.4157871
ATCGGAC1850.046.2221682
ATGCCGT324450.045.4996648-49
GCCGTCT326750.045.48750-51
TCGTATG326900.045.39035444-45
TTGTGTT264300.045.3269421
CGTCTTC328550.045.2461952-53
TCTCGTA325500.045.2426742-43
GTATGCC327950.045.08571646-47
Produced by FastQC (version 0.11.2)