FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n01_30749-1.34100000035870.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n01_30749-1.34100000035870.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5141247
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT129422225.173309121308506TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATATCGTAT167060.32494062238207966TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCCCATTCAGAAATCTCGTAT96960.18859237846382404TruSeq Adapter, Index 27 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGACATCTCGTAT84780.1649016279513511TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAACTCTCGTAT73450.1428641728358898TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGCAATCTCGTAT69810.1357841784298634TruSeq Adapter, Index 13 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATG69460.13510340973697627TruSeq Adapter, Index 13 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCAGAAATCTCGTAT61430.11948463086873672TruSeq Adapter, Index 27 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTCT56790.1104595830544613TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1508750.091.8193741
TCGGAAG1515450.091.076163
ATCGGAA1517750.090.956922
CGGAAGA1518200.090.560724
GAAGAGC1527300.089.928216
GAGCACA1540150.089.073079
GGAAGAG1554700.088.4685755
AGAGCAC1556550.088.174258
AAGAGCA1561900.087.7445147
TCGGACG7050.051.212953
TTGTGTT259300.048.3534051
GCGCACA8750.047.765219
GCCGTCT1474850.046.62724750-51
ATGCCGT1472400.046.56382448-49
GTATGCC1476200.046.51846746-47
TCTCGTA1457500.046.44563342-43
TCGTATG1482650.046.41380744-45
AATCTCG1450350.046.2674640-41
CGTCTTC1486200.046.24649452-53
GTCACAT1477700.046.16802628-29
Produced by FastQC (version 0.11.2)