FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n01_30521-1.341000000356df.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n01_30521-1.341000000356df.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3162340
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT701292.217629982860793TruSeq Adapter, Index 27 (97% over 39bp)
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC40290.1274056553058811No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA37720.1192787619294573No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA132850.055.8159034
TCGGAAG133250.055.7909433
GAAGAGC134050.055.2703176
ATCGGAA138200.053.827022
GATCGGA139050.053.66731
GGAAGAG156650.047.326755
ATGCCGT85700.043.1749748-49
TATGCCG85850.042.9335548-49
GCCGTCT86350.042.9066750-51
TGCCGTC86900.042.4711350-51
GTATGCC88200.041.94952846-47
TCGTATG88900.041.83259244-45
GATCTCG84850.041.7002540-41
TCGATCT85050.041.54634538-39
CGTATGC89300.041.35301646-47
CTCGATC85150.041.2744538-39
GAGCACA180500.041.099649
TACTCGA89400.040.77322436-37
ACTCGAT89700.040.71627836-37
TCTCGTA87950.040.63735642-43
Produced by FastQC (version 0.11.2)