##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n01_30521-1.341000000356df.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3162340 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.69182567339375 33.0 33.0 33.0 33.0 33.0 2 32.7155040255001 33.0 33.0 33.0 33.0 33.0 3 32.74970717886122 33.0 33.0 33.0 33.0 33.0 4 36.67234579456984 37.0 37.0 37.0 37.0 37.0 5 36.66366614595521 37.0 37.0 37.0 37.0 37.0 6 36.52980166585503 37.0 37.0 37.0 37.0 37.0 7 36.45479961041507 37.0 37.0 37.0 37.0 37.0 8 36.37497043328674 37.0 37.0 37.0 37.0 37.0 9 36.38795891649854 37.0 37.0 37.0 37.0 37.0 10-11 36.32772519716413 37.0 37.0 37.0 37.0 37.0 12-13 36.328852685037035 37.0 37.0 37.0 37.0 37.0 14-15 38.164404365122024 40.0 37.0 40.0 37.0 40.0 16-17 38.14344947096138 40.0 37.0 40.0 37.0 40.0 18-19 38.115608694827245 40.0 37.0 40.0 37.0 40.0 20-21 38.04833778151622 40.0 37.0 40.0 35.0 40.0 22-23 37.91824882839922 40.0 37.0 40.0 33.0 40.0 24-25 37.917659865795585 40.0 37.0 40.0 33.0 40.0 26-27 37.82471461006723 40.0 37.0 40.0 33.0 40.0 28-29 37.6619052663534 40.0 37.0 40.0 33.0 40.0 30-31 37.5940608852938 40.0 37.0 40.0 33.0 40.0 32-33 37.48545444196387 40.0 37.0 40.0 33.0 40.0 34-35 37.35871901819539 40.0 37.0 40.0 33.0 40.0 36-37 37.27004101393272 40.0 37.0 40.0 33.0 40.0 38-39 37.19893860242732 40.0 37.0 40.0 33.0 40.0 40-41 37.09820892124186 38.5 37.0 40.0 33.0 40.0 42-43 36.864377011959505 37.0 37.0 40.0 33.0 40.0 44-45 36.696507175066564 37.0 37.0 40.0 33.0 40.0 46-47 36.668228432110396 37.0 37.0 40.0 33.0 40.0 48-49 36.43859325689205 37.0 37.0 40.0 33.0 40.0 50-51 36.275368714306495 37.0 37.0 40.0 33.0 40.0 52-53 36.105172593712254 37.0 37.0 40.0 33.0 40.0 54-55 35.85776987926662 37.0 37.0 40.0 30.0 40.0 56-57 35.65312853772839 37.0 35.0 40.0 27.0 40.0 58-59 35.37560256012952 37.0 33.0 40.0 27.0 40.0 60-61 35.086742412264336 37.0 33.0 38.5 27.0 40.0 62-63 34.75690754314843 37.0 33.0 37.0 27.0 40.0 64-65 34.49016250624537 37.0 33.0 37.0 27.0 40.0 66-67 34.1048298095714 37.0 33.0 37.0 27.0 40.0 68-69 33.838028643346384 37.0 33.0 37.0 27.0 38.5 70-71 33.466851761670156 37.0 33.0 37.0 27.0 37.0 72-73 32.99408143969339 37.0 33.0 37.0 27.0 37.0 74-75 32.585442741767174 35.0 33.0 37.0 22.0 37.0 76-77 29.878882884193352 33.0 27.0 33.0 22.0 37.0 78-79 32.315221797782655 33.0 33.0 37.0 24.5 37.0 80-81 32.7608211008304 37.0 33.0 37.0 27.0 37.0 82-83 32.691551667436144 37.0 33.0 37.0 27.0 37.0 84-85 32.52258375127279 33.0 33.0 37.0 22.0 37.0 86-87 32.42542152330236 33.0 33.0 37.0 22.0 37.0 88-89 32.30296109842712 33.0 33.0 37.0 22.0 37.0 90-91 32.169112587514306 33.0 33.0 37.0 22.0 37.0 92-93 32.04443070637566 33.0 33.0 37.0 22.0 37.0 94-95 31.918219261685966 33.0 33.0 37.0 22.0 37.0 96-97 31.734204576358014 33.0 33.0 37.0 22.0 37.0 98-99 31.553469898872354 33.0 33.0 37.0 22.0 37.0 100-101 29.725886210843868 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.10574176035289184 2306 2 0.12380708914304961 2306 3 0.08004969865845624 2306 4 0.133440539006493 2306 5 0.10395304575594366 2306 6 0.19697243648810314 2306 7 0.22526827010552353 2306 8 0.2144349762087714 2306 9 0.15448609019224335 2306 10-11 0.21305428059839215 2306 12-13 0.20849967405459324 2306 14-15 0.4270861263155652 2306 16-17 0.40255268742350836 2306 18-19 0.4249065389376341 2306 20-21 0.4882135092878954 2306 22-23 0.5490824742517475 2306 24-25 0.47291390588490856 2306 26-27 0.6109283538690704 2306 28-29 0.518853241500274 2306 30-31 0.40449999264330216 2306 32-33 0.5609171206180932 2306 34-35 0.5298369221153791 2306 36-37 0.5206411877132027 2306 38-39 0.6045178315245323 2306 40-41 0.5941959490236925 2306 42-43 0.6326757887519392 2306 44-45 0.6460465767293968 2306 46-47 0.5679255410514017 2306 48-49 0.6654646880692354 2306 50-51 0.6707337149970485 2306 52-53 0.6450515370797518 2306 54-55 0.6064609943100976 2306 56-57 0.6996069058052186 2306 58-59 0.6158569771868585 2306 60-61 0.5906757758343417 2306 62-63 0.6678207919705059 2306 64-65 0.5845005369223131 2306 66-67 0.7920892004085758 2306 68-69 0.9071925596319446 2306 70-71 0.8964629396049588 2306 72-73 0.6058157988993145 2306 74-75 0.6658495654809187 2306 76-77 0.7014798061280914 2306 78-79 0.7155768133517455 2306 80-81 0.7987014599999398 2306 82-83 0.6098795913721702 2306 84-85 0.849031649177725 2306 86-87 0.9143747983101136 2306 88-89 0.846538200436683 2306 90-91 0.7962621270342467 2306 92-93 0.8921517508285106 2306 94-95 0.6007735795626701 2306 96-97 0.7362453901004677 2306 98-99 0.7919341427750091 2306 100-101 0.7412002027274518 2307 1 0.036844361850072005 2307 2 0.08806070556995849 2307 3 0.08292354539044311 2307 4 0.16159561661837074 2307 5 0.13335184624609298 2307 6 0.17950660729854917 2307 7 0.16911570586783853 2307 8 0.27084009010361854 2307 9 0.3281420966817592 2307 10-11 0.24652906418859288 2307 12-13 0.17653387466589976 2307 14-15 0.5081597014388208 2307 16-17 0.5238115430772368 2307 18-19 0.42624328427295666 2307 20-21 0.47786528708595455 2307 22-23 0.5972615407137312 2307 24-25 0.5666475427717117 2307 26-27 0.43289195775940925 2307 28-29 0.4094885486831643 2307 30-31 0.4670743895631304 2307 32-33 0.5581024546890987 2307 34-35 0.5634259423338293 2307 36-37 0.4133640703621282 2307 38-39 0.46906509585613065 2307 40-41 0.5403016838202888 2307 42-43 0.634904118560236 2307 44-45 0.6943831499942164 2307 46-47 0.565422689765775 2307 48-49 0.5735014242790939 2307 50-51 0.6644770497906833 2307 52-53 0.6397941266835332 2307 54-55 0.6048876099460259 2307 56-57 0.6810893756600436 2307 58-59 0.6975225287064859 2307 60-61 0.7002643960141413 2307 62-63 0.7134618185612922 2307 64-65 0.5809274643728841 2307 66-67 0.7866625817420569 2307 68-69 0.8071802015353811 2307 70-71 0.6864925653634089 2307 72-73 0.6566883916380704 2307 74-75 0.6176757183785284 2307 76-77 0.6055225536932589 2307 78-79 0.5638836194303067 2307 80-81 0.5485164252844683 2307 82-83 0.609950557825826 2307 84-85 0.6564759207285462 2307 86-87 0.6374446707186578 2307 88-89 0.5223998914485719 2307 90-91 0.466719928848768 2307 92-93 0.6871101356960096 2307 94-95 0.51818622179443 2307 96-97 0.6913884460119313 2307 98-99 0.7501024910295406 2307 100-101 0.7089172851525838 2201 1 0.10520076267032152 2201 2 0.09416588410841342 2201 3 0.09649884504199235 2201 4 0.16120357903899674 2201 5 0.13785181141960834 2201 6 0.10055273098304696 2201 7 0.12915311398502638 2201 8 0.171106944045178 2201 9 0.22290349053351122 2201 10-11 0.2194781517027522 2201 12-13 0.2170391526355573 2201 14-15 0.4536455500643868 2201 16-17 0.3924925331207021 2201 18-19 0.35973620171630216 2201 20-21 0.4901614323373096 2201 22-23 0.47147275279679235 2201 24-25 0.408485141444082 2201 26-27 0.540900387685646 2201 28-29 0.4507306144751837 2201 30-31 0.32470308300764117 2201 32-33 0.41461494333854176 2201 34-35 0.4347165223769096 2201 36-37 0.46590812905977685 2201 38-39 0.40066683935300773 2201 40-41 0.43402675013438596 2201 42-43 0.4567629773634465 2201 44-45 0.37062225393753323 2201 46-47 0.46756218613008826 2201 48-49 0.5447043949462511 2201 50-51 0.46745627978670257 2201 52-53 0.5547147830061974 2201 54-55 0.5153982428870236 2201 56-57 0.5621384004865391 2201 58-59 0.46721898234796555 2201 60-61 0.5420580621949398 2201 62-63 0.5423775135477484 2201 64-65 0.3838058892700431 2201 66-67 0.5723800169024926 2201 68-69 0.4907994847999504 2201 70-71 0.3810128577013856 2201 72-73 0.3468822122056352 2201 74-75 0.30329752448638914 2201 76-77 -0.04082120862780769 2201 78-79 0.29199501433644315 2201 80-81 0.40791679629834476 2201 82-83 0.3339870943223886 2201 84-85 0.46991124347420055 2201 86-87 0.44029753489345325 2201 88-89 0.21559853468542656 2201 90-91 0.4141498301652078 2201 92-93 0.4773823318285437 2201 94-95 0.29902155898189164 2201 96-97 0.3538076661415097 2201 98-99 0.24135864716976485 2201 100-101 0.21453249659407803 1101 1 0.11683738478594563 1101 2 0.09883062705342383 1101 3 0.049059603054409706 1101 4 0.13809715943396128 1101 5 0.15167830897284773 1101 6 0.14315677877709732 1101 7 0.13766026326646852 1101 8 0.13853413355795396 1101 9 0.11152175952250332 1101 10-11 0.1272495197613992 1101 12-13 0.19624530918959238 1101 14-15 0.3715759938662515 1101 16-17 0.31865253848809516 1101 18-19 0.3072476437096867 1101 20-21 0.28469212323303594 1101 22-23 0.36555437718510575 1101 24-25 0.37392887077233183 1101 26-27 0.2910323060721467 1101 28-29 0.298024882235957 1101 30-31 0.4038257063611539 1101 32-33 0.4027926079388351 1101 34-35 0.31396012380628946 1101 36-37 0.3783248980777856 1101 38-39 0.3455031940656781 1101 40-41 0.4077692490705829 1101 42-43 0.29273931244400586 1101 44-45 0.39692176337642593 1101 46-47 0.45549324041336803 1101 48-49 0.45464750533096066 1101 50-51 0.4292839644543136 1101 52-53 0.4904424224619177 1101 54-55 0.5016074071198204 1101 56-57 0.4441114885312558 1101 58-59 0.4837436868991247 1101 60-61 0.41578949993309067 1101 62-63 0.4866192533109981 1101 64-65 0.29833441023676244 1101 66-67 0.5004990027538341 1101 68-69 0.566845523561291 1101 70-71 0.4368963875170593 1101 72-73 0.34871365647170194 1101 74-75 0.4370985812841255 1101 76-77 0.05983644910608632 1101 78-79 0.44912719021311887 1101 80-81 0.5034125616123646 1101 82-83 0.28480631808015033 1101 84-85 0.4016903978909383 1101 86-87 0.5125387935248185 1101 88-89 0.3590240426543261 1101 90-91 0.5374267312406218 1101 92-93 0.6329413091694036 1101 94-95 0.584648438781123 1101 96-97 0.5573013727429306 1101 98-99 0.652842769817294 1101 100-101 0.45845941531905154 2304 1 0.0037495162350325018 2304 2 0.05181744809730304 2304 3 0.08982515868856922 2304 4 0.12514813309815764 2304 5 0.12381866784017603 2304 6 0.15596071720896987 2304 7 0.16225528838187842 2304 8 0.13775950232994916 2304 9 0.09393572377727111 2304 10-11 0.11595090287747922 2304 12-13 0.09938186095032364 2304 14-15 0.23405007149407453 2304 16-17 0.2836240349330268 2304 18-19 0.25429152602985994 2304 20-21 0.24669235758454988 2304 22-23 0.2931094995342818 2304 24-25 0.2453964639381141 2304 26-27 0.2737335443766753 2304 28-29 0.2637801297199971 2304 30-31 0.27112913850533715 2304 32-33 0.17931674946061804 2304 34-35 0.26365234399943205 2304 36-37 0.32511577472929076 2304 38-39 0.33597851862041495 2304 40-41 0.25882048693294024 2304 42-43 0.2218778274513511 2304 44-45 0.20099215615839938 2304 46-47 0.2705173554047846 2304 48-49 0.30155793441531387 2304 50-51 0.34101220734859794 2304 52-53 0.4056811017363984 2304 54-55 0.307869456324795 2304 56-57 0.32657121765628716 2304 58-59 0.27941293732803985 2304 60-61 0.3206324236229321 2304 62-63 0.4106427978996905 2304 64-65 0.2794698046105566 2304 66-67 0.2857190223420787 2304 68-69 0.19423823036544263 2304 70-71 0.21821962236380443 2304 72-73 0.0972666464432308 2304 74-75 0.2609329865176804 2304 76-77 0.18915385059001721 2304 78-79 0.330888286536009 2304 80-81 0.26023266034952286 2304 82-83 0.4015165479657128 2304 84-85 0.2657539480510067 2304 86-87 0.27146750313234236 2304 88-89 0.2330069073583303 2304 90-91 0.3455399639207144 2304 92-93 0.45966586544913923 2304 94-95 0.274691069752663 2304 96-97 0.4487803186917425 2304 98-99 0.438714303183648 2304 100-101 0.5059313484094865 2202 1 0.08128247267257649 2202 2 0.07152573197235057 2202 3 0.09010317166048765 2202 4 0.11879105506006482 2202 5 0.10583163367648751 2202 6 0.12499822515943748 2202 7 0.1288737289974975 2202 8 0.14223227022507245 2202 9 0.1312558002637516 2202 10-11 0.12543132245931332 2202 12-13 0.20382042160272817 2202 14-15 0.22558156342613245 2202 16-17 0.08353344017770326 2202 18-19 0.16746893577945343 2202 20-21 0.18201294350827624 2202 22-23 0.2334572335006797 2202 24-25 0.15917294353429412 2202 26-27 0.13842402326794456 2202 28-29 0.2129432549706607 2202 30-31 0.23983027101574095 2202 32-33 0.19504866291907774 2202 34-35 0.15569924868706408 2202 36-37 0.28073624499153027 2202 38-39 0.2816597719373277 2202 40-41 0.24162242218680774 2202 42-43 0.3100185739237702 2202 44-45 0.35493183797787253 2202 46-47 0.25684028190403296 2202 48-49 0.3030302890889729 2202 50-51 0.21302280830224873 2202 52-53 0.26415882626352527 2202 54-55 0.318867933077037 2202 56-57 0.289749944042903 2202 58-59 0.18777778555017477 2202 60-61 0.19749192022195672 2202 62-63 0.19283242097689168 2202 64-65 0.08481438877607417 2202 66-67 0.15470832112145416 2202 68-69 0.16223913985778182 2202 70-71 0.1477497905692431 2202 72-73 0.07634173931677424 2202 74-75 0.13757612420550203 2202 76-77 0.15218660750641178 2202 78-79 0.33279449842949305 2202 80-81 0.2957559070954048 2202 82-83 0.3196022286265432 2202 84-85 0.3447950868812697 2202 86-87 0.3040007178333042 2202 88-89 0.20511066038153558 2202 90-91 0.34016403366602077 2202 92-93 0.5419494761540093 2202 94-95 0.2877383096763424 2202 96-97 0.3247275377517873 2202 98-99 0.3757933591346436 2202 100-101 0.4611058078793455 1102 1 0.06102291748109678 1102 2 0.052538436598077 1102 3 0.06851093613445158 1102 4 0.13510826017483168 1102 5 0.11253689222426999 1102 6 0.07218939236558697 1102 7 0.04360737265502479 1102 8 0.21924667371967388 1102 9 0.14719948597679178 1102 10-11 0.12098935027687929 1102 12-13 0.05433763778473377 1102 14-15 0.2048089659161647 1102 16-17 0.24179865952664414 1102 18-19 0.14587386249917245 1102 20-21 0.08196809516733339 1102 22-23 0.14140545205316357 1102 24-25 0.06708687255638068 1102 26-27 0.049757091428908495 1102 28-29 0.15462385816370983 1102 30-31 0.14669287121211738 1102 32-33 0.17289366525549354 1102 34-35 0.09209146037596128 1102 36-37 0.02627967019873978 1102 38-39 0.2022627781595716 1102 40-41 0.1983151000241321 1102 42-43 0.253380310023978 1102 44-45 0.16745489473106545 1102 46-47 0.1410172243941048 1102 48-49 0.18129098694949164 1102 50-51 -0.021075658459132285 1102 52-53 0.03468051900284763 1102 54-55 0.05970458815936297 1102 56-57 0.0864030966932745 1102 58-59 -0.01869260153842589 1102 60-61 -0.10356046093762217 1102 62-63 -0.01507310119826144 1102 64-65 0.05217087408501442 1102 66-67 -0.004703114460575364 1102 68-69 0.030024504424069676 1102 70-71 -0.009212958703813001 1102 72-73 -0.028165891404370313 1102 74-75 -0.0932234532528895 1102 76-77 -0.16368836908211648 1102 78-79 0.12827879193274327 1102 80-81 0.2013782904495116 1102 82-83 0.06461097363918356 1102 84-85 0.1862965213660388 1102 86-87 0.1742962533837371 1102 88-89 0.01600661979787077 1102 90-91 0.06931032368071754 1102 92-93 0.06336996842869524 1102 94-95 -0.2529395375843748 1102 96-97 -0.003484398031726954 1102 98-99 0.13661637644305102 1102 100-101 0.2509135287464872 2305 1 0.08706430868870285 2305 2 0.06171082996084465 2305 3 0.07614648186662265 2305 4 0.028212828197432316 2305 5 0.08427386791262137 2305 6 0.006730437592018745 2305 7 0.02496336493092599 2305 8 0.07783151739216265 2305 9 0.03627804098449161 2305 10-11 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0.12324894847883527 1213 100-101 0.24085045520851267 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 17.0 10 19.0 11 17.0 12 30.0 13 108.0 14 316.0 15 790.0 16 1477.0 17 2490.0 18 3789.0 19 5066.0 20 6759.0 21 8733.0 22 11489.0 23 14705.0 24 18735.0 25 23260.0 26 29896.0 27 39610.0 28 56523.0 29 78776.0 30 80327.0 31 85138.0 32 113173.0 33 159833.0 34 244154.0 35 418359.0 36 733225.0 37 863052.0 38 162434.0 39 38.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.95290187616292 25.55325088257499 18.27423679400733 16.21961044725476 2 32.89298430908757 23.811671104308836 28.121422743917478 15.173921842686111 3 38.60106756389256 18.92149484242681 24.247487619927018 18.229949973753612 4 33.222206125998014 18.590327125181318 27.480071433496693 20.70739531532398 5 36.7654331918769 17.07238943314128 27.015659290272392 19.146518084709424 6 33.03152096232537 17.82581885565752 29.654780953344673 19.48787922867244 7 34.93846328984233 20.298607992815445 23.242756945805954 21.52017177153627 8 31.662376594547077 25.89515991322881 21.7127190624664 20.72974442975771 9 31.821119803689673 22.230152355534194 24.40385916757844 21.544868673197694 10-11 24.890128828652198 28.674415148276278 28.60924189049881 17.826214132572716 12-13 23.76012699456731 27.484789744303267 28.203134387826733 20.55194887330268 14-15 23.989814504449235 27.596953521759204 27.857441008873174 20.555790964918383 16-17 25.007858105074092 26.092229172068787 28.20288141060101 20.697031312256115 18-19 23.14858617352973 26.032384247108155 29.320819393234128 21.498210186127995 20-21 23.876117052562343 26.469797681463724 29.094183421137508 20.559901844836418 22-23 22.5240328364439 29.03193521253249 27.62914487373274 20.814887077290866 24-25 22.60398945084969 25.66087138005401 29.557558643283137 22.177580525813166 26-27 22.319690482364322 25.732052846942455 28.68175148782231 23.26650518287091 28-29 23.38979995825876 27.96087707204159 27.71724419259156 20.93207877710809 30-31 21.537279356425937 27.71559984062435 28.849570254937802 21.897550548011914 32-33 21.287353668485995 28.88715318403461 27.821391754207326 22.004101393272073 34-35 21.598752822277177 28.750940759058164 29.26396276175237 20.38634365691229 36-37 21.13493488998653 29.21216567478513 29.653769044441773 19.99913039078657 38-39 21.22267055408337 27.689906841136626 29.157332861109182 21.930089743670823 40-41 22.719647476235952 27.285412068278553 27.66193704661738 22.333003408868116 42-43 21.86306658993024 28.25602243907992 28.662161563905208 21.218749407084626 44-45 21.42478354636124 27.007975107040988 28.81170272646205 22.755538620135724 46-47 22.61455840123402 27.11436060460311 28.13352711394952 22.13755388021335 48-49 21.26026470223173 28.758116299165227 28.9648373419728 21.016781656630247 50-51 22.671383428531854 27.503629433783033 27.716905113588393 22.10808202409672 52-53 21.499136930413208 26.912955752050078 27.232173703644495 24.35573361389222 54-55 23.04527243520057 26.82034461553447 28.8870606068129 21.247322342452062 56-57 22.125673702655405 27.05688538622456 28.035423520052195 22.78201739106784 58-59 21.915030283977718 27.60990770431604 28.045088669703023 22.42997334200322 60-61 22.960444241353848 27.367677077519293 28.18967221072371 21.48220647040315 62-63 21.70095956446449 27.11476383405834 28.576689262263667 22.6075873392135 64-65 23.211743836955527 27.286495744984318 28.51255821808311 20.98920219997704 66-67 22.28966609589583 29.58907029716561 27.17007305318543 20.95119055375314 68-69 22.552127574274174 29.324904757845566 27.094445708769427 21.028521959110837 70-71 22.05113842400235 29.605745185263437 27.136420000357404 21.206696390376806 72-73 21.98952308299555 29.7793224636031 26.848815614357356 21.38233883904399 74-75 22.331247090139875 29.041590124693833 26.857218224327443 21.76994456083885 76-77 22.107538974821377 28.430073668919675 26.71825884158288 22.744128514676063 78-79 21.806950622585887 28.43518016630541 27.16483551930437 22.593033691804333 80-81 21.291211093454766 28.48296689830736 27.6964479124667 22.529374095771175 82-83 20.745797978855695 28.557932592157492 27.727940031321967 22.968329397664846 84-85 20.795924056248985 29.16594658601131 27.41846302084758 22.619666336892127 86-87 20.58603116439434 29.18226080306581 27.657233726315848 22.57447430622401 88-89 20.972730028563664 29.047392464841774 27.60092912944796 22.378948377146603 90-91 21.47620854168805 28.940131897410176 26.819835880188204 22.763823680713575 92-93 21.418372534895543 28.953675289771592 26.77368520514303 22.854266970189837 94-95 21.74035291468361 29.191383725137456 26.6164924612181 22.45177089896084 96-97 21.75890505064179 29.842107943247292 26.474570620963227 21.92441638514769 98-99 21.931832433722462 29.48411959729359 26.644639920264673 21.93940804871927 100-101 21.9987617939913 29.543626994788625 26.328908947580242 22.128702263639834 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 25.0 1 14.5 2 26.0 3 54.5 4 52.0 5 38.5 6 33.5 7 30.5 8 29.5 9 32.5 10 32.5 11 30.5 12 33.5 13 33.5 14 33.0 15 47.5 16 69.0 17 88.0 18 119.0 19 163.0 20 227.5 21 333.5 22 502.0 23 716.5 24 1011.5 25 1398.0 26 1911.5 27 2632.0 28 3607.0 29 4718.5 30 6302.5 31 8678.5 32 13356.5 33 22331.0 34 36901.5 35 57178.5 36 87340.0 37 123780.0 38 147318.0 39 152196.5 40 155646.5 41 159908.0 42 167600.5 43 182067.0 44 181370.5 45 170341.0 46 166277.5 47 161867.5 48 151881.0 49 135950.0 50 121350.0 51 117416.5 52 113648.0 53 102952.0 54 88676.0 55 73240.0 56 59302.0 57 45353.0 58 32656.0 59 23745.5 60 17921.5 61 14437.0 62 11505.0 63 8813.5 64 6616.0 65 4738.0 66 3366.5 67 2531.5 68 2011.5 69 1524.0 70 1100.0 71 821.0 72 582.0 73 392.5 74 306.0 75 255.5 76 205.5 77 150.5 78 99.5 79 66.5 80 48.0 81 42.5 82 35.0 83 27.5 84 24.5 85 16.0 86 9.0 87 7.0 88 7.5 89 6.5 90 4.5 91 3.5 92 1.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18818343378637337 2 0.0 3 0.0 4 0.025234478266094092 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 3.162215321565676E-5 48-49 0.0033993814706831014 50-51 3.162215321565676E-5 52-53 0.002545583333860369 54-55 0.009312724122010917 56-57 0.013581714806124578 58-59 0.013818880955242006 60-61 0.0030041045554873923 62-63 0.0022767950315272867 64-65 0.009708001037206626 66-67 0.01688622981716071 68-69 0.02509217857662364 70-71 0.02107616511823523 72-73 0.016822985510729395 74-75 0.02137657557378397 76-77 0.01552647722888747 78-79 0.006514163562425293 80-81 0.0036365476198005273 82-83 7.114984473522772E-4 84-85 0.0024823390274290556 86-87 0.01563715476514227 88-89 0.014403890789731654 90-91 0.017850705490238244 92-93 0.018419904248120064 94-95 0.01758191718790516 96-97 0.01970060145335416 98-99 0.027843305906385778 100-101 0.016048242756945805 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3162340.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.68270412563348 #Duplication Level Percentage of deduplicated Percentage of total 1 85.44599849559302 40.742962649846845 2 7.231851046181206 6.896684274314227 3 2.1430971732087625 3.065660052677847 4 1.3403017322478294 2.556368437513891 5 0.6279416305806634 1.4970977489572808 6 0.5120116667886978 1.464846048981475 7 0.343341238871422 1.1460007095064124 8 0.285798680782099 1.090212314818336 9 0.23832873607688246 1.022774274629116 >10 1.4652042295967285 14.029975834563016 >50 0.20580817987210795 6.937956291581132 >100 0.15142596788180376 13.896334696531037 >500 0.0071083175221553704 2.2370139300152596 >1k 0.0017166175695483633 1.199507400294939 >5k 0.0 0.0 >10k+ 6.628722700492301E-5 2.2166053357691458 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT 70129 2.217629982860793 TruSeq Adapter, Index 27 (97% over 39bp) GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 4029 0.1274056553058811 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 3772 0.1192787619294573 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0015494855075671812 0.0 0.0 2 0.0017392184268611218 0.0 0.0 3 0.001992195652586376 0.0 0.0 4 0.002213550725095973 0.0 0.0 5 0.0025930165636838544 0.0 0.0 6 0.002782749482977795 0.0 0.0 7 0.0030989710151343624 0.0 0.0 8 0.00341519254729093 0.0 0.0 9 0.003731414079447498 0.0 0.0 10-11 0.005502254659524277 0.0 0.0 12-13 0.00771580538462025 0.0 0.0 14-15 0.009945167186324051 0.0 0.0 16-17 0.011937362838910427 0.0 0.0 18-19 0.014372268636515997 0.0 0.0 20-21 0.017392184268611218 0.0 0.0 22-23 0.02031723344105947 0.0 0.0 24-25 0.023843103524605197 0.0 0.0 26-27 0.02800141667246406 0.0 0.0 28-29 0.03359853779163531 0.0 0.0 30-31 0.06692828728093753 0.0 0.0 32-33 0.07459665943573429 0.0 0.0 34-35 0.08101595653851262 0.0 0.0 36-37 0.08824161854829018 0.0 0.0 38-39 0.09747528728726196 0.0 0.0 40-41 0.10761018739287995 0.0 0.0 42-43 0.11896254039730073 0.0 0.0 44-45 0.13252844412681747 0.0 0.0 46-47 0.1477545109001562 0.0 0.0 48-49 0.16454587425766995 0.0 0.0 50-51 0.18309226711865265 0.0 0.0 52-53 0.20393126608777046 0.0 0.0 54-55 0.22748977023343475 0.0 0.0 56-57 0.25307209218490107 0.0 0.0 58-59 0.28153203007899213 0.0 0.0 60-61 0.3137075709759229 0.0 0.0 62-63 0.3461360890985789 0.0 0.0 64-65 0.3830549529778582 0.0 0.0 66-67 0.4221873675822334 0.0 0.0 68-69 0.46462429719764475 0.0 0.0 70-71 0.5108716962755427 0.0 0.0 72-73 0.5637281253755131 0.0 0.0 74-75 0.6253280798396124 0.0 0.0 76-77 0.6961142698128602 0.0 0.0 78-79 0.7759127734525699 0.0 0.0 80-81 0.8663205094961326 0.0 0.0 82-83 0.9683493868464491 0.0 0.0 84-85 1.083786057160204 0.0 0.0 86-87 1.2149389376221404 0.0 0.0 88-89 1.3617922171556507 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAAGA 13285 0.0 55.815903 4 TCGGAAG 13325 0.0 55.790943 3 GAAGAGC 13405 0.0 55.270317 6 ATCGGAA 13820 0.0 53.82702 2 GATCGGA 13905 0.0 53.6673 1 GGAAGAG 15665 0.0 47.32675 5 ATGCCGT 8570 0.0 43.17497 48-49 TATGCCG 8585 0.0 42.93355 48-49 GCCGTCT 8635 0.0 42.90667 50-51 TGCCGTC 8690 0.0 42.47113 50-51 GTATGCC 8820 0.0 41.949528 46-47 TCGTATG 8890 0.0 41.832592 44-45 GATCTCG 8485 0.0 41.70025 40-41 TCGATCT 8505 0.0 41.546345 38-39 CGTATGC 8930 0.0 41.353016 46-47 CTCGATC 8515 0.0 41.27445 38-39 GAGCACA 18050 0.0 41.09964 9 TACTCGA 8940 0.0 40.773224 36-37 ACTCGAT 8970 0.0 40.716278 36-37 TCTCGTA 8795 0.0 40.637356 42-43 >>END_MODULE