##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n01_30484-1.34100000035806.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8634207 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.69286826225037 33.0 33.0 33.0 33.0 33.0 2 32.71909464297069 33.0 33.0 33.0 33.0 33.0 3 32.747177013476744 33.0 33.0 33.0 33.0 33.0 4 36.66542903129378 37.0 37.0 37.0 37.0 37.0 5 36.651890903241025 37.0 37.0 37.0 37.0 37.0 6 36.51962861210068 37.0 37.0 37.0 37.0 37.0 7 36.43024252256171 37.0 37.0 37.0 37.0 37.0 8 36.37420367614536 37.0 37.0 37.0 37.0 37.0 9 36.39886523452588 37.0 37.0 37.0 37.0 37.0 10-11 36.34320302953126 37.0 37.0 37.0 37.0 37.0 12-13 36.317776664376936 37.0 37.0 37.0 35.0 37.0 14-15 38.120350600813715 40.0 37.0 40.0 37.0 40.0 16-17 38.11730202901089 40.0 37.0 40.0 37.0 40.0 18-19 38.086447661030135 40.0 37.0 40.0 37.0 40.0 20-21 38.02398552640677 40.0 37.0 40.0 33.0 40.0 22-23 37.846451561793685 40.0 37.0 40.0 33.0 40.0 24-25 37.868643929894205 40.0 37.0 40.0 33.0 40.0 26-27 37.777324831336564 40.0 37.0 40.0 33.0 40.0 28-29 37.59442494255697 40.0 37.0 40.0 33.0 40.0 30-31 37.551876043740904 40.0 37.0 40.0 33.0 40.0 32-33 37.40874043209759 40.0 37.0 40.0 33.0 40.0 34-35 37.27597965858359 40.0 37.0 40.0 33.0 40.0 36-37 37.1053292444807 40.0 37.0 40.0 33.0 40.0 38-39 37.083033161007144 40.0 37.0 40.0 33.0 40.0 40-41 37.04260877692647 40.0 37.0 40.0 33.0 40.0 42-43 36.789615305725235 37.0 37.0 40.0 33.0 40.0 44-45 36.65793975057582 37.0 37.0 40.0 33.0 40.0 46-47 36.65125905598511 37.0 37.0 40.0 33.0 40.0 48-49 36.3652547940998 37.0 37.0 40.0 33.0 40.0 50-51 36.250900806524555 37.0 37.0 40.0 33.0 40.0 52-53 36.121149574014154 37.0 37.0 40.0 33.0 40.0 54-55 35.864246015876155 37.0 37.0 40.0 27.0 40.0 56-57 35.69149401908015 37.0 37.0 40.0 27.0 40.0 58-59 35.460117819737235 37.0 33.0 40.0 27.0 40.0 60-61 35.1140522806553 37.0 33.0 40.0 27.0 40.0 62-63 34.84292622356634 37.0 33.0 37.0 27.0 40.0 64-65 34.58975572394778 37.0 33.0 37.0 27.0 40.0 66-67 34.107190388185046 37.0 33.0 37.0 27.0 40.0 68-69 33.85846401412428 37.0 33.0 37.0 27.0 40.0 70-71 33.46888469317449 37.0 33.0 37.0 27.0 38.5 72-73 32.858999326747664 37.0 33.0 37.0 22.0 37.0 74-75 32.29104270953893 37.0 33.0 37.0 22.0 37.0 76-77 29.757173762454386 33.0 27.0 35.0 18.5 37.0 78-79 31.946192394970378 33.0 33.0 37.0 22.0 37.0 80-81 32.28613559994566 37.0 33.0 37.0 22.0 37.0 82-83 32.18388880414843 35.0 33.0 37.0 22.0 37.0 84-85 31.97534122126097 33.0 33.0 37.0 22.0 37.0 86-87 31.85127128640766 33.0 33.0 37.0 22.0 37.0 88-89 31.700133144827312 33.0 33.0 37.0 18.5 37.0 90-91 31.54563018931559 33.0 33.0 37.0 15.0 37.0 92-93 31.41435577117852 33.0 33.0 37.0 15.0 37.0 94-95 31.276610753019938 33.0 33.0 37.0 15.0 37.0 96-97 31.09597360823061 33.0 33.0 37.0 15.0 37.0 98-99 30.917379094571164 33.0 33.0 37.0 15.0 37.0 100-101 29.1891958346609 33.0 24.5 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.0795186818992164 2306 2 0.08582395459826841 2306 3 0.10792768047092949 2306 4 0.1190916177007395 2306 5 0.14376300365941574 2306 6 0.22018167848448655 2306 7 0.25188111511562283 2306 8 0.2214752148957615 2306 9 0.20296816428248832 2306 10-11 0.26000963563548396 2306 12-13 0.22901946136907725 2306 14-15 0.44436588378317055 2306 16-17 0.4122832274111303 2306 18-19 0.33179483903758467 2306 20-21 0.42772777398564443 2306 22-23 0.4830158842242014 2306 24-25 0.4368943090592481 2306 26-27 0.40695372855226 2306 28-29 0.3803448681725854 2306 30-31 0.3636949091692827 2306 32-33 0.49291472672404524 2306 34-35 0.500494238665901 2306 36-37 0.5390237503390907 2306 38-39 0.54141395500546 2306 40-41 0.4635011383620906 2306 42-43 0.5637673790796711 2306 44-45 0.5290020545196654 2306 46-47 0.43614935162467816 2306 48-49 0.5395559643492476 2306 50-51 0.5196901347454244 2306 52-53 0.46626552100783414 2306 54-55 0.4996331932894833 2306 56-57 0.4718900505822532 2306 58-59 0.4305731187262367 2306 60-61 0.4849005602265919 2306 62-63 0.418283675370553 2306 64-65 0.43644661905086934 2306 66-67 0.6001843781347489 2306 68-69 0.5707843802318635 2306 70-71 0.4736060128149404 2306 72-73 0.3267553863232635 2306 74-75 0.40146366735578454 2306 76-77 0.458098362646588 2306 78-79 0.5990906851068516 2306 80-81 0.6324900636656494 2306 82-83 0.5725544958433488 2306 84-85 0.6831760075966855 2306 86-87 0.6447982233968048 2306 88-89 0.6422739532865869 2306 90-91 0.6823407881513255 2306 92-93 0.8103591037188131 2306 94-95 0.6430842032482254 2306 96-97 0.6778517109384197 2306 98-99 0.8037374136337014 2306 100-101 0.5511942990150196 2307 1 0.08710721715491587 2307 2 0.10292469547943028 2307 3 0.08781356624763959 2307 4 0.1539663326965126 2307 5 0.15180544656767836 2307 6 0.1671772209923219 2307 7 0.17813683792271462 2307 8 0.25491398031888224 2307 9 0.21139529257377632 2307 10-11 0.21151447205128449 2307 12-13 0.2009806551321347 2307 14-15 0.3951252485634811 2307 16-17 0.4245544639053449 2307 18-19 0.3892179982608184 2307 20-21 0.41451420572831665 2307 22-23 0.43882691927647244 2307 24-25 0.4392470817587437 2307 26-27 0.4094193560030419 2307 28-29 0.3540082697338036 2307 30-31 0.386074828684265 2307 32-33 0.42754407190247434 2307 34-35 0.45624384199714285 2307 36-37 0.44974198156430134 2307 38-39 0.3852705881381482 2307 40-41 0.3780501554112732 2307 42-43 0.37108164645216135 2307 44-45 0.3946865331934717 2307 46-47 0.3803868100318084 2307 48-49 0.3599660355591894 2307 50-51 0.4165539304218484 2307 52-53 0.4049973609146633 2307 54-55 0.3236846696633435 2307 56-57 0.40715367625159615 2307 58-59 0.41068257701477506 2307 60-61 0.4757970337915296 2307 62-63 0.4456247135563629 2307 64-65 0.23808507960385583 2307 66-67 0.35554831955933963 2307 68-69 0.5249774022159173 2307 70-71 0.35386272599551205 2307 72-73 0.13515730082891508 2307 74-75 0.16724111053671464 2307 76-77 0.2534266276871051 2307 78-79 0.3221963861869668 2307 80-81 0.2932175191167019 2307 82-83 0.26720740959505207 2307 84-85 0.32628615199450195 2307 86-87 0.2532876706660403 2307 88-89 0.27383444722034866 2307 90-91 0.2115236824223281 2307 92-93 0.33294471535477754 2307 94-95 0.13810680016697674 2307 96-97 0.32059031650381087 2307 98-99 0.47419153119827584 2307 100-101 0.3331333703253705 2201 1 0.10397008925819762 2201 2 0.11101937640973603 2201 3 0.09604883270739606 2201 4 0.14418751771213323 2201 5 0.12542003698730042 2201 6 0.12445083867527273 2201 7 0.21734465144247395 2201 8 0.24359970041603418 2201 9 0.18462186989854956 2201 10-11 0.1909552041758289 2201 12-13 0.18027459687969127 2201 14-15 0.34749034980151094 2201 16-17 0.3249174425416683 2201 18-19 0.34099449184115826 2201 20-21 0.3959481647797318 2201 22-23 0.3826257835322835 2201 24-25 0.3274043095297756 2201 26-27 0.3844816906182089 2201 28-29 0.40492535979479527 2201 30-31 0.3717304819443328 2201 32-33 0.3652279476766864 2201 34-35 0.44557716524450797 2201 36-37 0.3519966723610537 2201 38-39 0.38148319617572923 2201 40-41 0.37695671789780505 2201 42-43 0.36489391787552705 2201 44-45 0.4332253232427661 2201 46-47 0.4869767832997951 2201 48-49 0.43806311413156607 2201 50-51 0.4745838522731489 2201 52-53 0.46637282036693506 2201 54-55 0.5033652994865179 2201 56-57 0.474007558742116 2201 58-59 0.41446335624127784 2201 60-61 0.41398206842607266 2201 62-63 0.453225268610872 2201 64-65 0.3939624187170452 2201 66-67 0.4568978664806309 2201 68-69 0.44474005913459536 2201 70-71 0.32458624063472996 2201 72-73 0.30169187346064774 2201 74-75 0.2754835014469066 2201 76-77 0.1005694301270097 2201 78-79 0.4051800417533613 2201 80-81 0.4889601260223273 2201 82-83 0.39175480490264647 2201 84-85 0.4563450463801644 2201 86-87 0.5514811354882525 2201 88-89 0.39491039101906367 2201 90-91 0.49278762865146675 2201 92-93 0.4952955273975128 2201 94-95 0.4526731519017879 2201 96-97 0.4368853263998034 2201 98-99 0.539798634863704 2201 100-101 0.5514230211612983 1101 1 0.0993467595191504 1101 2 0.09692478647694003 1101 3 0.09189976511109421 1101 4 0.13171201185559767 1101 5 0.14798980827082175 1101 6 0.16520941762890828 1101 7 0.18798329226297028 1101 8 0.16618378535117984 1101 9 0.15166338671600954 1101 10-11 0.15253885605376638 1101 12-13 0.1965889511485841 1101 14-15 0.34455578102238604 1101 16-17 0.34868783130490044 1101 18-19 0.3354956583801254 1101 20-21 0.35484758479651646 1101 22-23 0.3461417446048216 1101 24-25 0.28337483489077897 1101 26-27 0.3278282034539828 1101 28-29 0.2668461761876628 1101 30-31 0.3382073897146327 1101 32-33 0.3129548837331626 1101 34-35 0.27400177394446956 1101 36-37 0.3716061724376871 1101 38-39 0.3859966910986401 1101 40-41 0.38542004438368593 1101 42-43 0.45084135023001437 1101 44-45 0.48450078848879485 1101 46-47 0.43933782317476755 1101 48-49 0.4111528411380121 1101 50-51 0.39050280120394376 1101 52-53 0.28724540731932535 1101 54-55 0.43615044495584243 1101 56-57 0.44595253136412794 1101 58-59 0.34093468850991115 1101 60-61 0.33905584385062326 1101 62-63 0.400236083017127 1101 64-65 0.4056050781002938 1101 66-67 0.4596635625412162 1101 68-69 0.5610355064139227 1101 70-71 0.4131783548596957 1101 72-73 0.31684914563665956 1101 74-75 0.3167918286484692 1101 76-77 0.09072296852721351 1101 78-79 0.44875565407401297 1101 80-81 0.4725722107972459 1101 82-83 0.4157156820923973 1101 84-85 0.4973589608842275 1101 86-87 0.5388821161253787 1101 88-89 0.3507310587296324 1101 90-91 0.40910670460156595 1101 92-93 0.5697725843162011 1101 94-95 0.4436547745646138 1101 96-97 0.45635776937807293 1101 98-99 0.6331036826921697 1101 100-101 0.46753532935482056 2304 1 0.08114439393479955 2304 2 0.06685922525009858 2304 3 0.06763138864932472 2304 4 0.09131467892797218 2304 5 0.12087718441018325 2304 6 0.13336997022555153 2304 7 0.1561902867827598 2304 8 0.11848567225417383 2304 9 0.118728495790144 2304 10-11 0.14808671726284217 2304 12-13 0.13830015720626676 2304 14-15 0.18274052552803965 2304 16-17 0.22162056307801947 2304 18-19 0.21072949285293419 2304 20-21 0.20604604578075225 2304 22-23 0.26469307760702065 2304 24-25 0.2515011205493707 2304 26-27 0.2243043660842048 2304 28-29 0.2457365828303395 2304 30-31 0.22833837119480194 2304 32-33 0.1431588492143021 2304 34-35 0.2363103695998987 2304 36-37 0.33521398241905587 2304 38-39 0.254680078444359 2304 40-41 0.26501066218759917 2304 42-43 0.23579266585158365 2304 44-45 0.3147951967164744 2304 46-47 0.27242713807217456 2304 48-49 0.32378838243998587 2304 50-51 0.3072346299776356 2304 52-53 0.15328242891640542 2304 54-55 0.19434874122686807 2304 56-57 0.2082446129796125 2304 58-59 0.2305756497246776 2304 60-61 0.21463173742892394 2304 62-63 0.28343571426459135 2304 64-65 0.1645634107852203 2304 66-67 0.25765257844663125 2304 68-69 0.26272673695217463 2304 70-71 0.3217540421040468 2304 72-73 0.09125356764007364 2304 74-75 0.30836057517486637 2304 76-77 0.1343504867275982 2304 78-79 0.30338050372515113 2304 80-81 0.3776915143612385 2304 82-83 0.3702692699384542 2304 84-85 0.3193340400628273 2304 86-87 0.3073884444019086 2304 88-89 0.30418901997071046 2304 90-91 0.3111670355280367 2304 92-93 0.40973295428195655 2304 94-95 0.3174783856993706 2304 96-97 0.38077839112984435 2304 98-99 0.46558357703160524 2304 100-101 0.34932406596180243 2202 1 0.07109237875272356 2202 2 0.06764750024655797 2202 3 0.07376920884187399 2202 4 0.09849864360818827 2202 5 0.09380109025721595 2202 6 0.08727148796591422 2202 7 0.09054679502558116 2202 8 0.10975167509597838 2202 9 0.1301139500075763 2202 10-11 0.10777161116021006 2202 12-13 0.08022260379868129 2202 14-15 0.14232220170070775 2202 16-17 0.09256854969935802 2202 18-19 0.09982124717186025 2202 20-21 0.14568437006371937 2202 22-23 0.16320775080984617 2202 24-25 0.05145022753111306 2202 26-27 0.10982009273717352 2202 28-29 0.15009319706961577 2202 30-31 0.10033027564223573 2202 32-33 0.15463860530910978 2202 34-35 0.21334014735221274 2202 36-37 0.16529691433684235 2202 38-39 0.25207349505630106 2202 40-41 0.20041230279919375 2202 42-43 0.22151077579794531 2202 44-45 0.15394192013246055 2202 46-47 0.20731364697269328 2202 48-49 0.29163305244340165 2202 50-51 0.1387406194580123 2202 52-53 0.2252071121075545 2202 54-55 0.23033707587576657 2202 56-57 0.20216445596059884 2202 58-59 0.15540043630284828 2202 60-61 0.07538413520298093 2202 62-63 0.09302323864423556 2202 64-65 -0.005196189999765011 2202 66-67 0.1821458316805078 2202 68-69 0.14539383658490124 2202 70-71 0.0668595363601554 2202 72-73 0.07737801571784075 2202 74-75 0.20055291891134175 2202 76-77 0.18266541734721642 2202 78-79 0.2601940071283124 2202 80-81 0.3526445069594146 2202 82-83 0.2869021594374104 2202 84-85 0.3232541145429977 2202 86-87 0.2345492197821386 2202 88-89 0.2167267660246246 2202 90-91 0.2055643630777908 2202 92-93 0.3116298159809894 2202 94-95 0.11307299837085694 2202 96-97 0.2870159731211359 2202 98-99 0.30437263603375797 2202 100-101 0.3059875089377897 1102 1 0.044929460237497665 1102 2 0.03454040588545837 1102 3 0.04919656688980467 1102 4 0.09088387828955291 1102 5 0.08377228655567848 1102 6 0.08929678538029862 1102 7 0.11296116024760039 1102 8 0.05469251202580239 1102 9 0.02873465211089865 1102 10-11 0.10036055099826768 1102 12-13 0.06537501614427299 1102 14-15 0.14661440966535366 1102 16-17 0.1021958452794891 1102 18-19 0.09537132928851122 1102 20-21 0.09097668461944153 1102 22-23 0.14283768722322066 1102 24-25 0.08346400152260003 1102 26-27 0.10799147645241902 1102 28-29 0.1485111500876286 1102 30-31 0.11861512410090569 1102 32-33 0.2106636600052667 1102 34-35 0.14673745207480238 1102 36-37 0.0902507441260596 1102 38-39 0.155793079888916 1102 40-41 0.15197554983461004 1102 42-43 0.09329881547554919 1102 44-45 0.13930229056173715 1102 46-47 0.2033293576198929 1102 48-49 0.1419451586813878 1102 50-51 0.059013048231271625 1102 52-53 0.06309566512683773 1102 54-55 0.10677866324628127 1102 56-57 0.17684293886585323 1102 58-59 0.16964096886039215 1102 60-61 0.005306773456347003 1102 62-63 0.054644281283110274 1102 64-65 0.028235072837922814 1102 66-67 0.07648376095446707 1102 68-69 -0.006173800676727126 1102 70-71 -0.10275544682889404 1102 72-73 -0.01347271769606806 1102 74-75 -0.15544321588125598 1102 76-77 -0.14520945162831111 1102 78-79 -0.004476397559784573 1102 80-81 0.0692895205347881 1102 82-83 0.12117366502413063 1102 84-85 -0.0015589400139859322 1102 86-87 -0.008129349655114027 1102 88-89 -0.09763633622374002 1102 90-91 -0.034295972474129854 1102 92-93 -0.046689764826414404 1102 94-95 -0.25248194145909864 1102 96-97 -0.08271779398396006 1102 98-99 0.09405757685796345 1102 100-101 -0.038294901220716326 2305 1 0.025763454361204197 2305 2 0.03767576887283042 2305 3 0.03587945184295904 2305 4 0.050978536680666764 2305 5 0.05015432111495244 2305 6 0.04304858208134732 2305 7 0.025966803256743276 2305 8 0.07029210998941693 2305 9 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-0.30227203989704776 1213 94-95 -0.1537397813410415 1213 96-97 -0.24477185518843925 1213 98-99 -0.07695318538244678 1213 100-101 0.012050854563320001 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 7.0 9 36.0 10 43.0 11 67.0 12 147.0 13 450.0 14 1292.0 15 3097.0 16 5582.0 17 9153.0 18 13668.0 19 17960.0 20 23437.0 21 30067.0 22 37927.0 23 47327.0 24 58803.0 25 74051.0 26 94635.0 27 127284.0 28 190258.0 29 264229.0 30 240742.0 31 233159.0 32 301242.0 33 423219.0 34 633921.0 35 1040648.0 36 1814606.0 37 2399877.0 38 546740.0 39 532.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.611793362039045 28.2793386755339 16.885417468060574 17.22345049436648 2 30.656793380098485 28.09419556422495 25.108513150078522 16.140497905598046 3 33.102437780331186 21.38729127064014 26.10779426529848 19.402476683730193 4 30.188808864548328 21.082496124575226 24.57668637598635 24.152008634890095 5 34.50111863197164 19.93141929536783 25.12124159172927 20.446220480931252 6 32.43248627233514 20.239148771855945 26.443331738514033 20.885033217294882 7 30.478965815853154 24.261382660851194 22.29787865868863 22.96177286460702 8 28.70139666561156 27.05348620898248 21.84648804458823 22.398629080817727 9 29.699681742631373 22.70310406039605 25.55673034014589 22.04048385682669 10-11 24.012384692653306 29.794670199591 28.404936318992586 17.78800878876311 12-13 21.429344929997622 29.28431064948987 28.74831469757443 20.538029722938074 14-15 21.577621430665257 29.33682270994893 28.88094413302808 20.20461172635773 16-17 23.870941477312275 26.886985683803964 28.83823609973678 20.40383673914698 18-19 21.386590569348172 26.679717083456534 31.19141688402884 20.74227546316645 20-21 23.51565696768678 27.133719402372446 30.739516668988827 18.611106960951943 22-23 21.34227845128105 31.500148189636874 28.195125504866862 18.962447854215217 24-25 21.328635044306907 26.68587862209002 30.611595251306806 21.373891082296268 26-27 21.305760911222073 26.91192138432632 28.635831871994732 23.14648583245688 28-29 23.058585461293664 29.586365024605037 28.497150925383185 18.857898588718108 30-31 20.6346628011119 27.926490527734625 30.651205142522066 20.787641528631408 32-33 20.349523702639978 30.247838626060275 28.55986658647401 20.84277108482574 34-35 22.626107991156573 30.278078809090403 28.65896080554937 18.436852394203658 36-37 22.481867761567447 29.966816871543617 29.024402588448485 18.52691277844045 38-39 20.58958049071559 27.471318443025517 33.04672334124025 18.89237772501864 40-41 20.575543301197204 27.464346175624467 28.249704923683204 23.710405599495125 42-43 20.64839307188257 29.47695138650255 30.43077378154126 19.443881760073623 44-45 20.48397727782065 27.186098271676833 30.709600777465724 21.620323673036797 46-47 22.731921259196856 27.366049578177577 28.386219131773842 21.515810030851725 48-49 20.464036437710018 29.953534970385675 30.304135002526372 19.27829358937793 50-51 22.854129756766145 27.905257243837617 29.705562796140605 19.535050203255636 52-53 20.564465106776304 27.766877348248542 27.57194365529659 24.09671388967857 54-55 22.85994379005688 27.71483322576595 29.80780457361385 19.617418410563317 56-57 20.722141054173523 27.41848962716355 30.01767871376064 21.841690604902293 58-59 20.77623425972217 27.66121144248755 30.04647427506803 21.51608002272225 60-61 22.553077194858577 27.836592700376034 29.933910016787873 19.676420087977515 62-63 20.197917443633337 27.887808483757944 29.90309015376875 22.011183918839972 64-65 22.368921332963833 27.641819034224095 29.98074610368494 20.00851352912713 66-67 20.657262393064673 31.469918174803603 27.833650790120462 20.039168642011262 68-69 20.492105100818208 31.837694577645557 27.800488820808855 19.86971150072738 70-71 20.30712520750529 32.22065125093979 27.734847335186064 19.73737620636886 72-73 20.318708508344073 32.08090716161352 27.579451096585313 20.0209332334571 74-75 20.581767113596452 31.53910375944867 27.559609527144723 20.319519599810153 76-77 20.75671375296225 30.96601775414286 27.727219968672976 20.550048524221914 78-79 20.62036419081436 30.64203416278204 28.024721072515934 20.712880573887666 80-81 20.35454588572161 30.75439522361272 27.837471165226287 21.053587725439385 82-83 20.202475172700947 30.63116845964482 27.80149626859405 21.364860099060184 84-85 20.40412711816753 30.365689975748687 27.971332758819482 21.258850147264297 86-87 20.406301001055628 30.139247035748713 28.507502849311585 20.94694911388408 88-89 20.634623230284753 30.47465239318578 28.15673113255521 20.733993243974265 90-91 20.82482487283066 30.1313198921667 27.771557160636956 21.272298074365683 92-93 20.548296694401802 30.26668531700213 27.627129982702105 21.557888005893968 94-95 20.218943054302233 30.24409767150375 27.58022197226563 21.956737301928385 96-97 20.153884871897272 30.58539443279425 27.65404053494802 21.606680160360455 98-99 20.455735697647363 30.29970044842083 27.579297349878253 21.66526650405356 100-101 20.68237054586099 30.26702795836635 27.141411481815307 21.909190013957346 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 34.0 1 23.0 2 19.5 3 39.5 4 48.0 5 38.0 6 40.5 7 50.0 8 52.0 9 53.0 10 60.0 11 65.0 12 68.0 13 85.0 14 100.0 15 107.0 16 150.5 17 246.0 18 390.0 19 568.5 20 880.0 21 1430.0 22 2321.5 23 3836.0 24 5868.5 25 8365.0 26 11997.5 27 17107.0 28 23249.0 29 30106.5 30 37835.5 31 47159.5 32 63955.5 33 93451.0 34 137581.5 35 201490.5 36 278838.0 37 369753.5 38 464226.5 39 522330.5 40 569688.5 41 581911.0 42 549774.5 43 541822.5 44 529591.5 45 516627.5 46 528836.5 47 499870.5 48 414017.5 49 331607.0 50 268897.5 51 219593.0 52 177724.5 53 141204.5 54 114152.5 55 91367.0 56 68042.0 57 47846.5 58 34049.5 59 24827.5 60 18051.0 61 12735.5 62 8591.5 63 5694.5 64 3854.0 65 2649.0 66 1890.0 67 1377.5 68 1011.5 69 735.0 70 513.0 71 373.5 72 274.5 73 213.5 74 176.0 75 135.5 76 104.0 77 79.5 78 68.5 79 53.5 80 38.0 81 31.0 82 25.5 83 22.0 84 15.5 85 10.5 86 7.0 87 3.5 88 5.0 89 5.0 90 2.0 91 1.5 92 1.5 93 0.0 94 0.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19005798679600802 2 0.0 3 0.0 4 0.02605913895740512 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 5.7909197683122487E-5 48-49 0.0034108517435359146 50-51 6.9491037219747E-5 52-53 0.002843341606241314 54-55 0.009948800161960444 56-57 0.014199335271901635 58-59 0.014581535976610245 60-61 0.0034340154226091633 62-63 0.0023511134259347733 64-65 0.010302046267827492 66-67 0.01778970552825523 68-69 0.02559007445617183 70-71 0.021970749600976672 72-73 0.0176333506945108 74-75 0.022051822477733046 76-77 0.01627248454895742 78-79 0.006960685561511324 80-81 0.0036772340528782783 82-83 7.180740512707189E-4 84-85 0.002785432408558192 86-87 0.01644621214200679 88-89 0.015299610027880961 90-91 0.01879732556794156 92-93 0.019162153513345232 94-95 0.018496197739989322 96-97 0.020609883455423295 98-99 0.02862451641476745 100-101 0.01711795883513101 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8634207.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.29405401052974 #Duplication Level Percentage of deduplicated Percentage of total 1 89.37713209134768 42.27006912434448 2 5.045074422127445 4.77204044414475 3 1.6786445240388383 2.3816971435311847 4 0.8358348445094613 1.5812007312045278 5 0.5088288540020911 1.2032289651645425 6 0.3967472491652666 1.125827149833073 7 0.22570768460957388 0.7472241998561759 8 0.1946476991122039 0.736454303587033 9 0.17835588047052234 0.7591655379661623 >10 1.2325897532026593 11.95946203467668 >50 0.189624710922006 6.312449599367423 >100 0.1140380413234973 9.954533306571971 >500 0.012795442943979506 4.263017570777978 >1k 0.009880655558885424 7.9678769281716635 >5k 7.368637656791907E-5 0.18545302582733914 >10k+ 2.4460289304534552E-5 3.7802999349748263 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT 326782 3.784736687457227 TruSeq Adapter, Index 7 (97% over 38bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.002837550686473002 0.0 0.0 2 0.003208169551644986 0.0 0.0 3 0.003648279454036717 0.0 1.1581839536624498E-5 4 0.0038451707261593334 0.0 1.1581839536624498E-5 5 0.00450533557974693 0.0 1.1581839536624498E-5 6 0.004899118123992163 0.0 2.3163679073248996E-5 7 0.005570864817116384 0.0 2.3163679073248996E-5 8 0.0062426115102406044 0.0 2.3163679073248996E-5 9 0.007122831315024066 0.0 2.3163679073248996E-5 10-11 0.010591592256243103 0.0 2.8954598841561247E-5 12-13 0.014199335271901634 0.0 3.47455186098735E-5 14-15 0.0182008608318054 0.0 4.632735814649799E-5 16-17 0.022584587096417773 0.0 4.632735814649799E-5 18-19 0.028624516414767445 0.0 4.632735814649799E-5 20-21 0.03564311117396189 0.0 4.632735814649799E-5 22-23 0.043982035640331534 0.0 4.632735814649799E-5 24-25 0.053438607621985434 0.0 5.790919768312249E-5 26-27 0.06403019987822854 0.0 5.790919768312249E-5 28-29 0.07572785781021929 0.0 5.790919768312249E-5 30-31 0.11033439434565329 0.0 5.790919768312249E-5 32-33 0.12533866746535033 0.0 5.790919768312249E-5 34-35 0.14000127631871695 0.0 5.790919768312249E-5 36-37 0.1550634586360971 0.0 5.790919768312249E-5 38-39 0.17236093598404578 0.0 5.790919768312249E-5 40-41 0.19008115047508126 0.0 5.790919768312249E-5 42-43 0.2089074306418644 0.0 5.790919768312249E-5 44-45 0.22910615879373752 0.0 5.790919768312249E-5 46-47 0.24930488694561065 0.0 5.790919768312249E-5 48-49 0.2697178791289113 0.0 5.790919768312249E-5 50-51 0.29161913769266823 0.0 5.790919768312249E-5 52-53 0.313647796491328 0.0 5.790919768312249E-5 54-55 0.3362208017482092 0.0 5.790919768312249E-5 56-57 0.3608669562821461 0.0 5.790919768312249E-5 58-59 0.38565788381029087 0.0 5.790919768312249E-5 60-61 0.41143326769904864 0.0 6.370011745143474E-5 62-63 0.43920072798810594 0.0 6.9491037219747E-5 64-65 0.4677557533656536 0.0 6.9491037219747E-5 66-67 0.4992409841459673 0.0 6.9491037219747E-5 68-69 0.5321391993497492 0.0 8.107287675637148E-5 70-71 0.5667399449654149 0.0 8.107287675637148E-5 72-73 0.6052148159060815 0.0 8.107287675637148E-5 74-75 0.6452011169062775 0.0 8.107287675637148E-5 76-77 0.6901618179874538 0.0 8.686379652468373E-5 78-79 0.7362112119850728 0.0 9.265471629299598E-5 80-81 0.788254207942895 0.0 9.265471629299598E-5 82-83 0.8459665143538949 0.0 9.265471629299598E-5 84-85 0.9068638266374665 0.0 9.265471629299598E-5 86-87 0.9750171613907335 0.0 9.265471629299598E-5 88-89 1.0482607146203466 0.0 9.265471629299598E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 48420 0.0 72.630455 3 CGGAAGA 48425 0.0 72.49542 4 GAAGAGC 49745 0.0 70.60981 6 GATCGGA 50100 0.0 70.35257 1 ATCGGAA 51290 0.0 68.57558 2 GGAAGAG 56480 0.0 62.341244 5 AGAGCAC 76485 0.0 45.99835 8 GAGCACA 76940 0.0 45.65226 9 AAGAGCA 77035 0.0 45.515816 7 TCCGTCG 65 2.5896152E-6 43.850414 2 GCCGTCT 39980 0.0 43.734314 50-51 ATGCCGT 39825 0.0 43.676773 48-49 CGTCTTC 40040 0.0 43.664364 52-53 CCGTCTT 39950 0.0 43.542763 52-53 TGCCGTC 39960 0.0 43.52441 50-51 TATGCCG 39905 0.0 43.482086 48-49 TCGTATG 40520 0.0 43.282406 44-45 TCTCGTA 39925 0.0 43.20176 42-43 CGTATGC 40270 0.0 43.191498 46-47 CGCGTTG 220 0.0 43.17426 6 >>END_MODULE