FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n01_30484-1.34100000035806.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n01_30484-1.34100000035806.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8634207
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT3267823.784736687457227TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG484200.072.6304553
CGGAAGA484250.072.495424
GAAGAGC497450.070.609816
GATCGGA501000.070.352571
ATCGGAA512900.068.575582
GGAAGAG564800.062.3412445
AGAGCAC764850.045.998358
GAGCACA769400.045.652269
AAGAGCA770350.045.5158167
TCCGTCG652.5896152E-643.8504142
GCCGTCT399800.043.73431450-51
ATGCCGT398250.043.67677348-49
CGTCTTC400400.043.66436452-53
CCGTCTT399500.043.54276352-53
TGCCGTC399600.043.5244150-51
TATGCCG399050.043.48208648-49
TCGTATG405200.043.28240644-45
TCTCGTA399250.043.2017642-43
CGTATGC402700.043.19149846-47
CGCGTTG2200.043.174266
Produced by FastQC (version 0.11.2)