##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n01_30423-1.3410000003574b.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2724480 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.6892662085976 33.0 33.0 33.0 33.0 33.0 2 32.706924257105946 33.0 33.0 33.0 33.0 33.0 3 32.741832202842374 33.0 33.0 33.0 33.0 33.0 4 36.65852529657035 37.0 37.0 37.0 37.0 37.0 5 36.658398666901576 37.0 37.0 37.0 37.0 37.0 6 36.543268807258634 37.0 37.0 37.0 37.0 37.0 7 36.41345504463237 37.0 37.0 37.0 37.0 37.0 8 36.346055761099365 37.0 37.0 37.0 37.0 37.0 9 36.3919797539347 37.0 37.0 37.0 37.0 37.0 10-11 36.316311185987786 37.0 37.0 37.0 35.0 37.0 12-13 36.301553140415784 37.0 37.0 37.0 35.0 37.0 14-15 38.08031532622739 40.0 37.0 40.0 35.0 40.0 16-17 38.08863177560489 40.0 37.0 40.0 37.0 40.0 18-19 38.098149738665725 40.0 37.0 40.0 37.0 40.0 20-21 38.077117468287526 40.0 37.0 40.0 37.0 40.0 22-23 37.83198298390886 40.0 37.0 40.0 33.0 40.0 24-25 37.92935716173362 40.0 37.0 40.0 33.0 40.0 26-27 37.84080943886539 40.0 37.0 40.0 33.0 40.0 28-29 37.61320618980503 40.0 37.0 40.0 33.0 40.0 30-31 37.604633177707306 40.0 37.0 40.0 33.0 40.0 32-33 37.50306553911206 40.0 37.0 40.0 33.0 40.0 34-35 37.47026772081278 40.0 37.0 40.0 33.0 40.0 36-37 37.34478762919896 40.0 37.0 40.0 33.0 40.0 38-39 37.03615185283063 38.5 37.0 40.0 33.0 40.0 40-41 36.874972471811134 37.0 37.0 40.0 33.0 40.0 42-43 36.64324348132487 37.0 37.0 40.0 33.0 40.0 44-45 36.41248183139535 37.0 37.0 40.0 33.0 40.0 46-47 36.50503508926474 37.0 37.0 40.0 33.0 40.0 48-49 36.01742901397698 37.0 37.0 40.0 33.0 40.0 50-51 35.987992571059436 37.0 37.0 40.0 33.0 40.0 52-53 35.99359529157858 37.0 37.0 40.0 33.0 40.0 54-55 35.48970555849189 37.0 35.0 40.0 27.0 40.0 56-57 35.346904730443974 37.0 33.0 40.0 27.0 40.0 58-59 35.21061119920132 37.0 33.0 40.0 27.0 40.0 60-61 34.82737660030538 37.0 33.0 38.5 27.0 40.0 62-63 34.5985573393822 37.0 33.0 37.0 27.0 40.0 64-65 34.34607301209772 37.0 33.0 37.0 27.0 40.0 66-67 33.496549249765096 37.0 33.0 37.0 27.0 40.0 68-69 33.509627525252526 37.0 33.0 37.0 27.0 37.0 70-71 33.186886304909564 37.0 33.0 37.0 27.0 37.0 72-73 32.25764567917548 35.0 33.0 37.0 22.0 37.0 74-75 31.30170803235847 33.0 30.0 37.0 15.0 37.0 76-77 28.794709999706363 33.0 27.0 33.0 15.0 37.0 78-79 30.835360142118866 33.0 30.0 37.0 15.0 37.0 80-81 31.214828150692977 33.0 33.0 37.0 15.0 37.0 82-83 31.00676385952549 33.0 33.0 37.0 15.0 37.0 84-85 30.829065546452902 33.0 33.0 37.0 15.0 37.0 86-87 30.73373230855062 33.0 33.0 37.0 15.0 37.0 88-89 30.64479900751703 33.0 33.0 37.0 15.0 37.0 90-91 30.52488162878788 33.0 33.0 37.0 15.0 37.0 92-93 30.349837033121915 33.0 27.0 37.0 15.0 37.0 94-95 30.16734514476157 33.0 27.0 37.0 15.0 37.0 96-97 29.990045439863753 33.0 27.0 37.0 15.0 37.0 98-99 29.810437221047685 33.0 27.0 37.0 15.0 37.0 100-101 28.1690937353183 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.20051112626232737 2306 2 0.1756419724801006 2306 3 0.15283960756482173 2306 4 0.20496250413393113 2306 5 0.2098871605342154 2306 6 0.23236494866866764 2306 7 0.22743788396060438 2306 8 0.2353810284201998 2306 9 0.27542454198342625 2306 10-11 0.32239867539271216 2306 12-13 0.26664860362316034 2306 14-15 0.37746548783673717 2306 16-17 0.47871707890234205 2306 18-19 0.4927267214678963 2306 20-21 0.49694468601722974 2306 22-23 0.5048719374474473 2306 24-25 0.4214744963684751 2306 26-27 0.38089993267250577 2306 28-29 0.33062243488890886 2306 30-31 0.35521330569973486 2306 32-33 0.5010958795979761 2306 34-35 0.5928464419090034 2306 36-37 0.5631219432522414 2306 38-39 0.5946762658257327 2306 40-41 0.5905582932300035 2306 42-43 0.5948461935092908 2306 44-45 0.6045149148219409 2306 46-47 0.3838619327217927 2306 48-49 0.17755966575343507 2306 50-51 0.22055907110528494 2306 52-53 0.3152580809634813 2306 54-55 0.2612201144006505 2306 56-57 0.3338236765961753 2306 58-59 0.35660202076418557 2306 60-61 0.19639181412062356 2306 62-63 0.1984194989113206 2306 64-65 0.07734517616244574 2306 66-67 0.17625677320126698 2306 68-69 0.5923379905732773 2306 70-71 0.7733001119953045 2306 72-73 0.31057203018518464 2306 74-75 0.18870620572850783 2306 76-77 0.4238811779267202 2306 78-79 0.5947243819272927 2306 80-81 0.5234474408345271 2306 82-83 0.491714923398316 2306 84-85 0.5144474924537583 2306 86-87 0.5220739588356942 2306 88-89 0.5254180012361012 2306 90-91 0.47957296178778464 2306 92-93 0.71004288514278 2306 94-95 0.554956324164916 2306 96-97 0.6190930556917529 2306 98-99 0.6385378771426495 2306 100-101 0.5823062101659424 2307 1 0.1185864405256325 2307 2 0.08425071738761858 2307 3 0.09283601287189924 2307 4 0.1568698699865294 2307 5 0.13433052778111687 2307 6 0.24397362821812862 2307 7 0.1889403566736192 2307 8 0.21264622961407298 2307 9 0.21863601885756623 2307 10-11 0.16301135267643474 2307 12-13 0.16502472832632264 2307 14-15 0.4700587864576775 2307 16-17 0.45537472252667044 2307 18-19 0.4625844043070444 2307 20-21 0.519650618349857 2307 22-23 0.5417897061235166 2307 24-25 0.5003219071002647 2307 26-27 0.4446218559421524 2307 28-29 0.45219413267327013 2307 30-31 0.4135584397926806 2307 32-33 0.5620161209870602 2307 34-35 0.5673532609325491 2307 36-37 0.5499433087998327 2307 38-39 0.6142278551157219 2307 40-41 0.5584881858249346 2307 42-43 0.5794742517215283 2307 44-45 0.6068648091597524 2307 46-47 0.4782610198876469 2307 48-49 0.364364419462504 2307 50-51 0.4167075754951668 2307 52-53 0.38191948267586184 2307 54-55 0.21753043443725772 2307 56-57 0.3529482599385858 2307 58-59 0.3853873413454352 2307 60-61 0.14210950000981626 2307 62-63 0.23328910959519078 2307 64-65 0.18662983227015673 2307 66-67 0.3224600149607042 2307 68-69 0.5625396201673993 2307 70-71 0.3138270068341953 2307 72-73 0.2710870516444075 2307 74-75 0.1366997134952257 2307 76-77 0.3570855198800551 2307 78-79 0.467290883746422 2307 80-81 0.5213312015369063 2307 82-83 0.45982999714398787 2307 84-85 0.6086205496856252 2307 86-87 0.5774512926628468 2307 88-89 0.49543635595604485 2307 90-91 0.5462283723452899 2307 92-93 0.6598691416513525 2307 94-95 0.32271519652063674 2307 96-97 0.4683602285202326 2307 98-99 0.5461896712212102 2307 100-101 0.6760359219369292 2201 1 0.15192748729844396 2201 2 0.123711289728206 2201 3 0.08575188540874734 2201 4 0.18645099477816274 2201 5 0.1828175965458385 2201 6 0.1431846294394532 2201 7 0.2599342966444951 2201 8 0.24112097375405028 2201 9 0.22104777677236598 2201 10-11 0.2535610137239317 2201 12-13 0.24618509123943255 2201 14-15 0.5063711108798756 2201 16-17 0.4448968926733272 2201 18-19 0.47284197073545897 2201 20-21 0.5135386880726003 2201 22-23 0.5679272527839103 2201 24-25 0.4837624015435509 2201 26-27 0.48790184670627923 2201 28-29 0.5346487330901013 2201 30-31 0.433988252219379 2201 32-33 0.47418418147392316 2201 34-35 0.6093809148789688 2201 36-37 0.5200800189655794 2201 38-39 0.5264094605373444 2201 40-41 0.6368756347592708 2201 42-43 0.5315658175058573 2201 44-45 0.624101181689511 2201 46-47 0.5807818714918724 2201 48-49 0.5138815108915722 2201 50-51 0.6032222131390483 2201 52-53 0.6756751918025685 2201 54-55 0.5885158170474867 2201 56-57 0.6890660689707033 2201 58-59 0.5932737340280312 2201 60-61 0.5140953089690186 2201 62-63 0.6584952457797613 2201 64-65 0.5229951211848842 2201 66-67 0.5310213757937063 2201 68-69 0.6971314593821845 2201 70-71 0.48647125152812265 2201 72-73 0.39086934023709574 2201 74-75 0.35098716384734274 2201 76-77 0.26764451807136425 2201 78-79 0.4485419091106415 2201 80-81 0.6155777436543097 2201 82-83 0.5248699888803081 2201 84-85 0.6126904861079794 2201 86-87 0.691672562716942 2201 88-89 0.6216808443068942 2201 90-91 0.5436090098230864 2201 92-93 0.81180659899114 2201 94-95 0.6150159011610583 2201 96-97 0.508204742796341 2201 98-99 0.5258775857974847 2201 100-101 0.44101218918583385 1101 1 0.060728128524644376 1101 2 0.08629624330522745 1101 3 0.07455784228293538 1101 4 0.13799907261682876 1101 5 0.12200574641781259 1101 6 0.11104965383597687 1101 7 0.1553566656864973 1101 8 0.2173106377485965 1101 9 0.18169712293720153 1101 10-11 0.20267488055439742 1101 12-13 0.15049442846811445 1101 14-15 0.24628210516765137 1101 16-17 0.24474181806067463 1101 18-19 0.20505071665062502 1101 20-21 0.3076512940062486 1101 22-23 0.3654220967099988 1101 24-25 0.38352780148851195 1101 26-27 0.33422531225764374 1101 28-29 0.32618052914082085 1101 30-31 0.3772742644265321 1101 32-33 0.2642876587238945 1101 34-35 0.3307768165980747 1101 36-37 0.3518221807391555 1101 38-39 0.4503843932807925 1101 40-41 0.40928993301525196 1101 42-43 0.41381532021024725 1101 44-45 0.44150040673500257 1101 46-47 0.4497926900911935 1101 48-49 0.544763960777459 1101 50-51 0.6071928347664155 1101 52-53 0.3935904951136493 1101 54-55 0.4405227357609718 1101 56-57 0.5419910879887979 1101 58-59 0.5411377957481491 1101 60-61 0.510504839884014 1101 62-63 0.6138145038073759 1101 64-65 0.5475000491463433 1101 66-67 0.6304172963477939 1101 68-69 0.6933926125638834 1101 70-71 0.5493972227587491 1101 72-73 0.19272280623840032 1101 74-75 0.35086756176754363 1101 76-77 0.23903419378544655 1101 78-79 0.5746443567154103 1101 80-81 0.5486126124216355 1101 82-83 0.27358769344119693 1101 84-85 0.4231765435190731 1101 86-87 0.5237425576628922 1101 88-89 0.49810531763395716 1101 90-91 0.599645671052027 1101 92-93 0.7042143259342559 1101 94-95 0.4964878333886382 1101 96-97 0.6451496473605793 1101 98-99 0.6263241839397296 1101 100-101 0.6227217559919147 2304 1 0.06817869676006438 2304 2 0.08077177730930885 2304 3 0.11036558270983221 2304 4 0.10336658424404987 2304 5 0.11313942538362198 2304 6 0.044115552374009326 2304 7 0.12561880578103768 2304 8 0.0812584898692208 2304 9 0.11826133539730677 2304 10-11 0.0924989548313917 2304 12-13 0.1565887020920229 2304 14-15 0.22150500190574007 2304 16-17 0.22361748660795655 2304 18-19 0.15038434473394346 2304 20-21 0.1162288998041845 2304 22-23 0.20732973260864895 2304 24-25 0.2129326383739354 2304 26-27 0.32174357135240683 2304 28-29 0.2916069272419719 2304 30-31 0.3107510342842801 2304 32-33 0.21540026052459638 2304 34-35 0.15093762050325665 2304 36-37 0.2323968860447465 2304 38-39 0.1090114046397872 2304 40-41 0.2744840249296061 2304 42-43 0.26312597685160455 2304 44-45 0.32182146188913663 2304 46-47 0.383964568083762 2304 48-49 0.45506147087058935 2304 50-51 0.4194156848145738 2304 52-53 0.4298493173651181 2304 54-55 0.5104973040459342 2304 56-57 0.4637568818080027 2304 58-59 0.42694672724450555 2304 60-61 0.4726506515597322 2304 62-63 0.4475664583523553 2304 64-65 0.3638840194477382 2304 66-67 0.470202922902736 2304 68-69 0.36343473830842044 2304 70-71 0.18083535813475038 2304 72-73 -0.029801841752934877 2304 74-75 0.11910536021381546 2304 76-77 0.09110003229357844 2304 78-79 0.20490295218724697 2304 80-81 0.3364840950744252 2304 82-83 0.31980461863345866 2304 84-85 0.3349862353941653 2304 86-87 0.26329378174879636 2304 88-89 0.24342979830385403 2304 90-91 0.24713866041720323 2304 92-93 0.37735085012678127 2304 94-95 0.2437527091315097 2304 96-97 0.39747342387021334 2304 98-99 0.4168678547395679 2304 100-101 0.395172605319722 2202 1 0.06389854282778629 2202 2 0.0901105346550537 2202 3 0.05309497177023559 2202 4 0.09071401192461792 2202 5 0.12524326903286465 2202 6 0.06701662566408828 2202 7 0.06824010126931768 2202 8 0.11571590847168522 2202 9 0.1006765324747434 2202 10-11 0.19521588237267196 2202 12-13 0.13827255387904103 2202 14-15 0.15727562558807762 2202 16-17 0.09070466363447593 2202 18-19 0.10546742299280965 2202 20-21 0.1775468680316976 2202 22-23 0.15677419316460117 2202 24-25 0.19272889517262115 2202 26-27 0.22081045139402988 2202 28-29 0.2861667447308136 2202 30-31 0.17399139835750077 2202 32-33 0.17069196816576238 2202 34-35 0.19791324144809863 2202 36-37 0.06926988840083936 2202 38-39 0.18862434177063392 2202 40-41 0.22875859842137203 2202 42-43 0.19469307879263198 2202 44-45 0.16586616517228236 2202 46-47 0.3661208678738106 2202 48-49 0.5403719938237685 2202 50-51 0.35665936903019 2202 52-53 0.4615018395924082 2202 54-55 0.5146130327152534 2202 56-57 0.4900728331676518 2202 58-59 0.44332879144504744 2202 60-61 0.533695749428361 2202 62-63 0.5516681260692025 2202 64-65 0.34564731561621187 2202 66-67 0.34478573004886215 2202 68-69 0.31004635634616307 2202 70-71 0.2962714717577981 2202 72-73 0.10453931034878394 2202 74-75 0.1382453044797174 2202 76-77 0.1809841436809272 2202 78-79 0.2553375674400442 2202 80-81 0.40214373390128344 2202 82-83 0.3509672045480734 2202 84-85 0.34756542711788896 2202 86-87 0.18830619493339995 2202 88-89 0.22133476911419336 2202 90-91 0.22890081413329355 2202 92-93 0.2909948953573469 2202 94-95 0.15349317031317256 2202 96-97 0.30406757117450667 2202 98-99 0.440349821128617 2202 100-101 0.30100904304771703 1102 1 0.06800768908109944 1102 2 0.08973824580959189 1102 3 0.0924367417015759 1102 4 0.12568155035142325 1102 5 0.10194354240202586 1102 6 0.057398514104129106 1102 7 0.0764051168382025 1102 8 0.121764952491759 1102 9 0.11485193379789393 1102 10-11 0.12278190598019734 1102 12-13 0.1073313225645407 1102 14-15 0.09569625675735693 1102 16-17 0.025649218260390683 1102 18-19 0.051134611058778034 1102 20-21 -0.008470940759323753 1102 22-23 0.12304948549742534 1102 24-25 0.08493373200388277 1102 26-27 0.05539766070579333 1102 28-29 0.07526446405555731 1102 30-31 0.012945854697207437 1102 32-33 0.09552421890521856 1102 34-35 0.01913029461620397 1102 36-37 0.06209764308653121 1102 38-39 0.1600905620805193 1102 40-41 0.08423670208724587 1102 42-43 0.1407534279190017 1102 44-45 0.3122855651245331 1102 46-47 0.2800957497468275 1102 48-49 0.3161736249427065 1102 50-51 0.34302372156108873 1102 52-53 0.12551196870776238 1102 54-55 0.3190032635440829 1102 56-57 0.2262951409361733 1102 58-59 0.19079058392163262 1102 60-61 0.23279710717812208 1102 62-63 0.42991375549236466 1102 64-65 0.2678182371285729 1102 66-67 0.35764317688156666 1102 68-69 0.22303171655835996 1102 70-71 -0.005949997873536006 1102 72-73 -0.043558231927598 1102 74-75 -0.10302661743708441 1102 76-77 -0.1312131076838874 1102 78-79 0.03370242025437875 1102 80-81 0.23165655853732048 1102 82-83 0.2068173534148876 1102 84-85 -0.10162299294477606 1102 86-87 -0.054118571695749296 1102 88-89 -0.04660152921066896 1102 90-91 0.015459351356494011 1102 92-93 -0.07501011397423696 1102 94-95 -0.20029339057927942 1102 96-97 -0.058393709648555614 1102 98-99 -0.2198361446214605 1102 100-101 0.057004322840978006 2305 1 -0.009834637944081237 2305 2 0.023733449324510048 2305 3 0.03247205472197123 2305 4 0.05166557048593745 2305 5 0.008650306049638345 2305 6 -0.055061413810001625 2305 7 -0.057283892669452996 2305 8 -0.058249682731762675 2305 9 0.045106254684405656 2305 10-11 -0.041589255237930445 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1213 98-99 0.07001485541094254 1213 100-101 0.15344369694390636 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 4.0 10 11.0 11 11.0 12 30.0 13 66.0 14 264.0 15 812.0 16 1673.0 17 2938.0 18 4048.0 19 5265.0 20 7381.0 21 9992.0 22 14051.0 23 19172.0 24 24724.0 25 31614.0 26 41881.0 27 61345.0 28 93848.0 29 107647.0 30 74179.0 31 66223.0 32 87842.0 33 125357.0 34 193836.0 35 334729.0 36 599043.0 37 707284.0 38 109195.0 39 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.7102709981955 21.57085768227831 21.294207332208785 12.424663987317404 2 31.36756371858116 27.395172656800565 29.65420924359878 11.583054381019497 3 37.98313806671364 14.82433345078694 32.32220460418135 14.870323878318064 4 31.354408560304144 14.18052784136489 29.895529484329426 24.569534114001545 5 38.99621212121212 13.018337444209537 32.551532769556026 15.433917665022317 6 34.07861316654922 13.765305673009163 36.546827284472634 15.609253875968992 7 33.60120096311957 23.881034178999293 25.240963413201783 17.27680144467935 8 29.886473749119098 33.63669397463002 19.866910382898755 16.609921893352126 9 38.455742013154804 23.647264799154332 20.429513154803853 17.46748003288701 10-11 30.227915051679588 28.905332393704487 25.120353241719524 15.746399312896406 12-13 25.23369597134132 27.294107499412736 24.804696676062953 22.667499853182992 14-15 24.06668428470754 28.09594858468405 24.617321470519148 23.220045660089266 16-17 27.909619450317123 24.830426356589147 25.339716202724922 21.920237990368804 18-19 22.087719491425887 24.937878053793753 30.016975716466998 22.957426738313366 20-21 26.164882840028188 25.579560136246183 30.357022257458304 17.898534766267325 22-23 21.940278511862815 33.89410089264741 25.69462796570355 18.470992629786235 24-25 22.614627378435518 22.979467641531595 31.155523255813954 23.250381724218933 26-27 20.527972310312425 23.0579596840498 27.407193299271785 29.006874706365988 28-29 24.678213824289404 29.46002539934226 25.330430027014327 20.531330749354005 30-31 18.455815421658446 25.92839734554851 30.550435312426593 25.065351920366457 32-33 19.032420865633075 28.87250044045102 26.621740662438338 25.473338031477567 34-35 19.934519614752173 24.038073320413435 30.820688718581163 25.206718346253233 36-37 23.68837356119333 26.481750645994833 26.296834625322994 23.53304116748884 38-39 23.745485377026075 30.896409590086915 24.18911131665492 21.168993716232087 40-41 24.76127554615927 29.973407769556026 24.00799418604651 21.257322498238196 42-43 21.129848631665492 30.37963207657975 29.08892706131078 19.401592230443974 44-45 19.04187221047686 26.86464940098661 28.930676679586565 25.162801708949967 46-47 24.260321474484556 26.292637762653577 25.302539661274505 24.144501101587366 48-49 19.9241298052041 29.811664262458752 30.436960200265013 19.827245732072132 50-51 23.95828554125107 25.416401325025923 28.61259508712688 22.012718046596135 52-53 19.04515543170416 26.237441326789046 24.665010284788057 30.052392956718734 54-55 24.3472879212269 26.15190827351367 29.90306066357478 19.59774314168465 56-57 20.166226828662143 25.514085523009545 28.562935624088464 25.756752024239855 58-59 18.95948963797474 24.794432521060582 29.611665186976055 26.63441265398862 60-61 22.80362068911725 24.76838465444758 31.36883242301007 21.059162233425102 62-63 18.802430903616514 24.79952606408926 31.050877269612347 25.34716576268188 64-65 24.562809355512712 26.412922342314033 29.508037553611832 19.51623074856142 66-67 20.214279486602944 34.963092129716735 24.57923743007787 20.243390953602454 68-69 19.610489737701485 33.16696679828402 26.57712281165502 20.645420652359476 70-71 19.20737029432901 36.00456329739538 25.314093815593957 19.473972592681655 72-73 21.118708553841092 35.32868382564566 24.548148762709175 19.004458857804067 74-75 22.442918367961305 33.18969156791754 24.799258858418757 19.5681312057024 76-77 21.632454003934196 31.47487880701797 25.122569601417293 21.770097587630545 78-79 21.60746774359024 32.41096302229175 25.120182094249046 20.861387139868963 80-81 21.121922141494316 32.557706749944394 25.09181912348433 21.22855198507696 82-83 20.472526492396113 31.59228559337058 25.98365328041033 21.951534633822984 84-85 21.080713930198517 30.84159077261442 25.885035009389185 22.19266028779788 86-87 19.28780545108469 31.51265856474471 27.08299605916936 22.116539925001238 88-89 19.020995861178317 31.704578180302413 27.151250264079547 22.123175694439727 90-91 20.45423508050335 30.615557495523106 25.94090057731574 22.989306846657804 92-93 20.58452694503503 30.923624453258753 24.96579913108776 23.526049470618457 94-95 20.86095960840042 31.919099326837262 24.90419444590267 22.315746618859645 96-97 20.347498526039722 33.0972916576418 23.784505905753182 22.770703910565302 98-99 20.596941550658745 30.76435900349543 25.435101987167823 23.203597458678 100-101 21.76878352562154 31.445894988171965 24.841723824501585 21.94359766170491 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 285.0 1 190.5 2 136.0 3 263.0 4 290.5 5 213.0 6 195.0 7 220.0 8 256.5 9 259.5 10 262.0 11 253.5 12 223.5 13 216.5 14 228.5 15 245.0 16 244.0 17 246.5 18 287.0 19 307.5 20 333.0 21 384.0 22 409.0 23 434.5 24 522.5 25 674.5 26 869.5 27 1108.0 28 1399.5 29 1832.0 30 2506.5 31 3616.5 32 6013.0 33 10258.5 34 17005.0 35 28922.0 36 47147.0 37 71651.0 38 100287.0 39 121825.0 40 137306.5 41 146748.0 42 179684.5 43 221156.5 44 213904.5 45 188088.0 46 181284.5 47 168819.5 48 139116.5 49 115657.0 50 98222.0 51 89242.0 52 86805.0 53 86025.0 54 71855.0 55 44444.0 56 28538.0 57 22787.5 58 19881.5 59 16082.0 60 12101.5 61 9390.5 62 6940.5 63 4909.0 64 3625.0 65 2614.0 66 1867.0 67 1448.5 68 1146.0 69 873.5 70 657.0 71 508.5 72 331.0 73 174.0 74 117.0 75 85.0 76 56.5 77 35.5 78 19.0 79 14.5 80 11.5 81 9.0 82 8.0 83 3.5 84 2.0 85 2.0 86 1.0 87 1.5 88 1.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18950405214940097 2 0.0 3 0.0 4 0.027344667606295513 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 9.176062955132722E-5 48-49 0.0033033826638477802 50-51 1.8352125910265445E-4 52-53 0.0024958891237961004 54-55 0.00919441508104299 56-57 0.01361727742541696 58-59 0.013745742306788818 60-61 0.002991396523373268 62-63 0.002238959361052384 64-65 0.009836739487902278 66-67 0.01715923772609819 68-69 0.02477536997885835 70-71 0.021013184167253934 72-73 0.017030772844726334 74-75 0.021068240544984733 76-77 0.015250616631430585 78-79 0.006478300446323702 80-81 0.0037438336856941506 82-83 6.97380784590087E-4 84-85 0.0024958891237961004 86-87 0.01596634954193094 88-89 0.014516531595019968 90-91 0.01813190039934226 92-93 0.01833377378435518 94-95 0.017361111111111112 96-97 0.019306436457599246 98-99 0.02861096429410383 100-101 0.016296687808315714 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2724480.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.33275902376332 #Duplication Level Percentage of deduplicated Percentage of total 1 83.96619674783089 27.148588055895157 2 6.881623473746136 4.450037469378137 3 2.4971780484577257 2.422219682406457 4 1.4146256412986364 1.8295499987578183 5 0.9174561526432121 1.4831944349140997 6 0.6138302848548393 1.1908096009019709 7 0.40460087073641265 0.9157303718027673 8 0.31532728483732436 0.8156320891410228 9 0.2936248395824593 0.8544331063449736 >10 2.1014387857625887 13.589830589602206 >50 0.30770616090485853 6.961332216517466 >100 0.2540462335223407 16.381840741191724 >500 0.022432771973170344 4.886660769506885 >1k 0.008879582391958149 4.718207077645462 >5k 4.5916509224192253E-4 0.9378636273602751 >10k+ 5.739563650735104E-4 11.414070168633526 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT 227563 8.35252965703547 TruSeq Adapter, Index 6 (97% over 36bp) TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 29632 1.0876203899459713 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 27173 0.9973646347192858 No Hit GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 12503 0.4589132605120977 No Hit TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT 10660 0.3912673244068593 No Hit TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 9345 0.3430012332628612 No Hit GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 5581 0.2048464294103829 No Hit TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 5233 0.19207334977683815 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATATCGTAT 5110 0.18755872680291286 TruSeq Adapter, Index 6 (97% over 36bp) TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 3820 0.140210241954428 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 3508 0.12875851538642236 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 3085 0.11323261686633779 No Hit GAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGC 2732 0.10027601597369039 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.004477918722104768 0.0 0.0 2 0.004734848484848485 0.0 0.0 3 0.005175299506694856 0.0 0.0 4 0.005579046276720696 0.0 0.0 5 0.0064232440685929055 0.0 0.0 6 0.007267441860465116 0.0 0.0 7 0.008111639652337327 0.0 0.0 8 0.008919133192389007 0.0 0.0 9 0.010424007517030773 0.0 0.0 10-11 0.01627833568240545 0.0 0.0 12-13 0.021783973455485085 0.0 0.0 14-15 0.028794485553206482 0.0 0.0 16-17 0.03641061780596665 0.0 0.0 18-19 0.044650722339675826 0.0 0.0 20-21 0.050505050505050504 0.0 0.0 22-23 0.05612080103359173 0.0 0.0 24-25 0.06313131313131314 0.0 0.0 26-27 0.06911410617805966 0.0 0.0 28-29 0.07619802677942214 0.0 0.0 30-31 0.12778585271317827 0.0 0.0 32-33 0.13769600070472163 0.0 0.0 34-35 0.1455874148461358 0.0 0.0 36-37 0.1539743363871271 0.0 0.0 38-39 0.1636275546159267 0.0 0.0 40-41 0.17487740779891942 0.0 0.0 42-43 0.18671452901104063 0.0 0.0 44-45 0.1988452842377261 0.0 0.0 46-47 0.21257267441860467 0.0 0.0 48-49 0.22692403688043222 0.0 0.0 50-51 0.24235817477096547 0.0 0.0 52-53 0.25940729974160204 0.0 0.0 54-55 0.27884220108057317 0.0 0.0 56-57 0.30033254052149405 0.0 0.0 58-59 0.3246491073525957 0.0 0.0 60-61 0.3522690568475452 0.0 0.0 62-63 0.38146728917077755 0.0 0.0 64-65 0.4112894937749589 0.0 0.0 66-67 0.4460667723749119 0.0 0.0 68-69 0.48407402513507164 0.0 0.0 70-71 0.5269262391355415 0.0 0.0 72-73 0.5744398931172188 0.0 0.0 74-75 0.6244677883486023 0.0 0.0 76-77 0.6798545043457833 0.0 0.0 78-79 0.7439768322762509 0.0 0.0 80-81 0.8164310253699789 0.0 0.0 82-83 0.8996028599953019 0.0 0.0 84-85 0.9908312778952313 0.0 0.0 86-87 1.0930709713413203 0.0 0.0 88-89 1.2031653746770026 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 31375 0.0 81.4287 3 CGGAAGA 31340 0.0 81.14069 4 GATCGGA 31610 0.0 81.11493 1 GAAGAGC 31470 0.0 80.71076 6 ATCGGAA 31870 0.0 80.149055 2 GGAAGAG 33225 0.0 76.54755 5 GAGCACA 40140 0.0 63.194897 9 AGAGCAC 40245 0.0 62.994617 8 AAGAGCA 40335 0.0 62.795185 7 GACGTAC 35 2.790425E-4 54.278614 7 GCCGTCT 26725 0.0 45.749195 50-51 ATGCCGT 26555 0.0 45.681805 48-49 TCGTATG 27045 0.0 45.615597 44-45 TCTCGTA 26510 0.0 45.49705 42-43 GTATGCC 27050 0.0 45.353405 46-47 CTGCTTG 26735 0.0 45.308167 58-59 AATCTCG 26850 0.0 45.20312 40-41 TTGTGTT 24715 0.0 45.025883 1 TGCCGTC 26775 0.0 44.998493 50-51 TATGCCG 26830 0.0 44.98344 48-49 >>END_MODULE