FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n01_30423-1.3410000003574b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n01_30423-1.3410000003574b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2724480
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT2275638.35252965703547TruSeq Adapter, Index 6 (97% over 36bp)
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA296321.0876203899459713No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC271730.9973646347192858No Hit
GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC125030.4589132605120977No Hit
TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT106600.3912673244068593No Hit
TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT93450.3430012332628612No Hit
GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC55810.2048464294103829No Hit
TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT52330.19207334977683815No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATATCGTAT51100.18755872680291286TruSeq Adapter, Index 6 (97% over 36bp)
TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT38200.140210241954428No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC35080.12875851538642236No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG30850.11323261686633779No Hit
GAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGC27320.10027601597369039No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG313750.081.42873
CGGAAGA313400.081.140694
GATCGGA316100.081.114931
GAAGAGC314700.080.710766
ATCGGAA318700.080.1490552
GGAAGAG332250.076.547555
GAGCACA401400.063.1948979
AGAGCAC402450.062.9946178
AAGAGCA403350.062.7951857
GACGTAC352.790425E-454.2786147
GCCGTCT267250.045.74919550-51
ATGCCGT265550.045.68180548-49
TCGTATG270450.045.61559744-45
TCTCGTA265100.045.4970542-43
GTATGCC270500.045.35340546-47
CTGCTTG267350.045.30816758-59
AATCTCG268500.045.2031240-41
TTGTGTT247150.045.0258831
TGCCGTC267750.044.99849350-51
TATGCCG268300.044.9834448-49
Produced by FastQC (version 0.11.2)