##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n01_30245-1.34100000035771.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2289338 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.712104547253396 33.0 33.0 33.0 33.0 33.0 2 32.746830306402984 33.0 33.0 33.0 33.0 33.0 3 32.775666153272255 33.0 33.0 33.0 33.0 33.0 4 36.71390332052322 37.0 37.0 37.0 37.0 37.0 5 36.712368378981175 37.0 37.0 37.0 37.0 37.0 6 36.58799181248029 37.0 37.0 37.0 37.0 37.0 7 36.53678967456968 37.0 37.0 37.0 37.0 37.0 8 36.47008174415486 37.0 37.0 37.0 37.0 37.0 9 36.48608811804985 37.0 37.0 37.0 37.0 37.0 10-11 36.446156268755416 37.0 37.0 37.0 37.0 37.0 12-13 36.42578072787854 37.0 37.0 37.0 37.0 37.0 14-15 38.31507754643482 40.0 37.0 40.0 37.0 40.0 16-17 38.31113579558807 40.0 37.0 40.0 37.0 40.0 18-19 38.33017929200494 40.0 37.0 40.0 37.0 40.0 20-21 38.28175088169593 40.0 37.0 40.0 37.0 40.0 22-23 38.17494424152309 40.0 37.0 40.0 37.0 40.0 24-25 38.16190859541055 40.0 37.0 40.0 37.0 40.0 26-27 38.09071268637484 40.0 37.0 40.0 37.0 40.0 28-29 37.96946846643003 40.0 37.0 40.0 33.0 40.0 30-31 37.93169029649619 40.0 37.0 40.0 33.0 40.0 32-33 37.83399131102529 40.0 37.0 40.0 33.0 40.0 34-35 37.7659928328626 40.0 37.0 40.0 33.0 40.0 36-37 37.68895374994867 40.0 37.0 40.0 33.0 40.0 38-39 37.58884708155807 40.0 37.0 40.0 33.0 40.0 40-41 37.496999132500314 40.0 37.0 40.0 33.0 40.0 42-43 37.36811908071242 40.0 37.0 40.0 33.0 40.0 44-45 37.235455839198934 40.0 37.0 40.0 33.0 40.0 46-47 37.21092822466582 40.0 37.0 40.0 33.0 40.0 48-49 37.000280648816386 38.5 37.0 40.0 33.0 40.0 50-51 36.8691407734463 37.0 37.0 40.0 33.0 40.0 52-53 36.7654241095024 37.0 37.0 40.0 33.0 40.0 54-55 36.57828376587467 37.0 37.0 40.0 33.0 40.0 56-57 36.43366204553456 37.0 37.0 40.0 33.0 40.0 58-59 36.231582448725355 37.0 37.0 40.0 33.0 40.0 60-61 35.989742667967775 37.0 37.0 40.0 33.0 40.0 62-63 35.76013174987704 37.0 37.0 40.0 27.0 40.0 64-65 35.542927911911654 37.0 33.0 40.0 27.0 40.0 66-67 35.20415705326168 37.0 33.0 38.5 27.0 40.0 68-69 34.991435297016 37.0 33.0 37.0 27.0 40.0 70-71 34.69029125450239 37.0 33.0 37.0 27.0 40.0 72-73 34.324970362611374 37.0 33.0 37.0 27.0 40.0 74-75 33.906808867891066 37.0 33.0 37.0 27.0 38.5 76-77 31.33040796072926 33.0 30.0 35.0 24.5 37.0 78-79 33.55781584021233 37.0 33.0 37.0 27.0 37.0 80-81 33.87013210805918 37.0 33.0 37.0 27.0 37.0 82-83 33.75602990908288 37.0 33.0 37.0 27.0 37.0 84-85 33.55963929310569 37.0 33.0 37.0 27.0 37.0 86-87 33.42856799651253 37.0 33.0 37.0 27.0 37.0 88-89 33.28288527076386 37.0 33.0 37.0 27.0 37.0 90-91 33.13054647238634 37.0 33.0 37.0 27.0 37.0 92-93 32.992929833864636 37.0 33.0 37.0 27.0 37.0 94-95 32.8646119533245 35.0 33.0 37.0 27.0 37.0 96-97 32.69994928664968 33.0 33.0 37.0 27.0 37.0 98-99 32.5361825121498 33.0 33.0 37.0 27.0 37.0 100-101 30.900444801073498 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.139346719976011 2306 2 0.12189260068457486 2306 3 0.14262737477859133 2306 4 0.1931086127390813 2306 5 0.15191011776821028 2306 6 0.1247495078372296 2306 7 0.19075160302681127 2306 8 0.22354607691502792 2306 9 0.16437075329552187 2306 10-11 0.22555581577631045 2306 12-13 0.26248559842813535 2306 14-15 0.4170583589439403 2306 16-17 0.43498821957661704 2306 18-19 0.4191634970898832 2306 20-21 0.42458469694503265 2306 22-23 0.46594205308640113 2306 24-25 0.40799378780847917 2306 26-27 0.48661733175174504 2306 28-29 0.35339753761849835 2306 30-31 0.41563747648819316 2306 32-33 0.47471859114923376 2306 34-35 0.4661232738190435 2306 36-37 0.3835063197344013 2306 38-39 0.5271177990273301 2306 40-41 0.36947402022128273 2306 42-43 0.48238033376969014 2306 44-45 0.42713768765526794 2306 46-47 0.5515416384665386 2306 48-49 0.552901672714853 2306 50-51 0.4010470901210681 2306 52-53 0.43339335630718523 2306 54-55 0.42819625417168083 2306 56-57 0.4046493458281546 2306 58-59 0.33593455344847456 2306 60-61 0.4432160172001929 2306 62-63 0.38671235257935166 2306 64-65 0.4908021315165456 2306 66-67 0.4854970275625021 2306 68-69 0.5543595641676475 2306 70-71 0.6549298491310367 2306 72-73 0.4193933469266611 2306 74-75 0.44166639268792096 2306 76-77 0.5714899985221109 2306 78-79 0.6649740823673866 2306 80-81 0.5926073575067505 2306 82-83 0.39571943529366393 2306 84-85 0.4440040953960178 2306 86-87 0.41917470952002844 2306 88-89 0.2666508585062104 2306 90-91 0.41161902656786253 2306 92-93 0.4187512434646905 2306 94-95 0.34013189213104056 2306 96-97 0.3572319918746132 2306 98-99 0.5559219283436221 2306 100-101 0.542009550156159 2307 1 0.0919072931122571 2307 2 0.12035005559655332 2307 3 0.10461664537910309 2307 4 0.15723867180368245 2307 5 0.15283214446083804 2307 6 0.19211473502055298 2307 7 0.21609739286915897 2307 8 0.24982781533709186 2307 9 0.22785455836205415 2307 10-11 0.23814498049093658 2307 12-13 0.20134842687031096 2307 14-15 0.445329932797371 2307 16-17 0.5116516762538907 2307 18-19 0.5093114947048676 2307 20-21 0.4626978516565856 2307 22-23 0.5831884072424955 2307 24-25 0.49965628785322735 2307 26-27 0.5414397342159987 2307 28-29 0.4544008283548422 2307 30-31 0.5537350386862059 2307 32-33 0.5920506643529038 2307 34-35 0.5966493182887334 2307 36-37 0.5410511671047757 2307 38-39 0.4328932739923985 2307 40-41 0.6673423406490215 2307 42-43 0.5749813678874531 2307 44-45 0.6280816313758422 2307 46-47 0.5912659532809812 2307 48-49 0.4987077440100265 2307 50-51 0.48052058026258493 2307 52-53 0.5133395939569851 2307 54-55 0.369873690412426 2307 56-57 0.4906621452133635 2307 58-59 0.6265998883871973 2307 60-61 0.6307299142267553 2307 62-63 0.38263721734811185 2307 64-65 0.4349481172199603 2307 66-67 0.5806133489067093 2307 68-69 0.7366531513089072 2307 70-71 0.5350936262105961 2307 72-73 0.25927891697026695 2307 74-75 0.34235520788361384 2307 76-77 0.46010813985472154 2307 78-79 0.4217066075152829 2307 80-81 0.3590750605186983 2307 82-83 0.4794271679472999 2307 84-85 0.5322257016078851 2307 86-87 0.6356668156111667 2307 88-89 0.5978060131644582 2307 90-91 0.368777601237106 2307 92-93 0.315544118004226 2307 94-95 0.31875384010535157 2307 96-97 0.6603536369484289 2307 98-99 0.40139077175700066 2307 100-101 0.4285968859325848 2201 1 0.12143054956766264 2201 2 0.06667167857614231 2201 3 0.06132603249766788 2201 4 0.12797782110211386 2201 5 0.07374961039654693 2201 6 0.12702767147511906 2201 7 0.1685821964450369 2201 8 0.13918465287065374 2201 9 0.1290681104264877 2201 10-11 0.20904571060512467 2201 12-13 0.1892922988759409 2201 14-15 0.38740274443029676 2201 16-17 0.34548901003252297 2201 18-19 0.3662861949137479 2201 20-21 0.3583157128409553 2201 22-23 0.35175995082943956 2201 24-25 0.31231945279462536 2201 26-27 0.291706985530638 2201 28-29 0.35923755532016344 2201 30-31 0.36856230562591463 2201 32-33 0.4127416189581936 2201 34-35 0.42157902038366046 2201 36-37 0.42898545980944647 2201 38-39 0.505372613266637 2201 40-41 0.5165904214342092 2201 42-43 0.3533126163871927 2201 44-45 0.4420706162979471 2201 46-47 0.5119288409072027 2201 48-49 0.535979797648146 2201 50-51 0.4728209841587159 2201 52-53 0.40037261235370636 2201 54-55 0.5612539595870558 2201 56-57 0.5445205534540918 2201 58-59 0.4538366365238957 2201 60-61 0.5098158401836059 2201 62-63 0.4484442194071008 2201 64-65 0.3587406780168436 2201 66-67 0.5008242948049002 2201 68-69 0.6220392381383775 2201 70-71 0.47801180183459024 2201 72-73 0.48762317393705956 2201 74-75 0.3747604548059442 2201 76-77 0.3764711513782082 2201 78-79 0.4292953148240244 2201 80-81 0.4346609633659426 2201 82-83 0.490518470240211 2201 84-85 0.6010095553047492 2201 86-87 0.5995025317179596 2201 88-89 0.5459829268255092 2201 90-91 0.5380693815259647 2201 92-93 0.6208383031821754 2201 94-95 0.5409190752194135 2201 96-97 0.5500776188961112 2201 98-99 0.5620755314991825 2201 100-101 0.5214054027884281 1101 1 0.11103824714155053 1101 2 0.10806758593682275 1101 3 0.09200331280621299 1101 4 0.1575808726982828 1101 5 0.13842088289051446 1101 6 0.1955226573835489 1101 7 0.13956911027444363 1101 8 0.09592021438868414 1101 9 0.2451920498714557 1101 10-11 0.17718220033541598 1101 12-13 0.16953490986625752 1101 14-15 0.2996229563999506 1101 16-17 0.25605870154205945 1101 18-19 0.34596004236097855 1101 20-21 0.28643226665624866 1101 22-23 0.3391254725125137 1101 24-25 0.32973756743719207 1101 26-27 0.3225529590780596 1101 28-29 0.35691256917820624 1101 30-31 0.42418313976327937 1101 32-33 0.4334996446388999 1101 34-35 0.39549686401086603 1101 36-37 0.5186093507051694 1101 38-39 0.42251335197709494 1101 40-41 0.46657932885511144 1101 42-43 0.5140505402251208 1101 44-45 0.335116951936655 1101 46-47 0.5444473049813823 1101 48-49 0.3686121373404134 1101 50-51 0.458238698116439 1101 52-53 0.46128915436969464 1101 54-55 0.40251024179701034 1101 56-57 0.5210506840945612 1101 58-59 0.43512766523922863 1101 60-61 0.39635734609493767 1101 62-63 0.5518373502429981 1101 64-65 0.4410025993129949 1101 66-67 0.5207025959994525 1101 68-69 0.6007224353085121 1101 70-71 0.44139860897858085 1101 72-73 0.24990567027743538 1101 74-75 0.4058599291221725 1101 76-77 0.2719977999935743 1101 78-79 0.5107607827956642 1101 80-81 0.5806101132626438 1101 82-83 0.37953199273940896 1101 84-85 0.5005267669314932 1101 86-87 0.48120708932378164 1101 88-89 0.6703259757422515 1101 90-91 0.6747082931872441 1101 92-93 0.8055165323517812 1101 94-95 0.6162300054351064 1101 96-97 0.6526517117935384 1101 98-99 0.6711816478428219 1101 100-101 0.6956501548616032 2304 1 0.06051800835724919 2304 2 0.09240575973747411 2304 3 0.11314278252837084 2304 4 0.12802779136077902 2304 5 0.07848534599487778 2304 6 0.12075550093565823 2304 7 0.0685953211635777 2304 8 0.1650915227577343 2304 9 0.07643257061524622 2304 10-11 0.17117638222948273 2304 12-13 0.12719853299683592 2304 14-15 0.18406412910014325 2304 16-17 0.2565735573019765 2304 18-19 0.285303069207842 2304 20-21 0.2283463813346458 2304 22-23 0.29870839356352263 2304 24-25 0.27149435351635987 2304 26-27 0.3004692402850324 2304 28-29 0.2846184129783751 2304 30-31 0.23955053672692372 2304 32-33 0.14672013054286737 2304 34-35 0.09129216701686715 2304 36-37 0.1376850410225572 2304 38-39 0.11445798895869785 2304 40-41 0.28233075188813217 2304 42-43 0.18215906199667842 2304 44-45 0.3537304235933689 2304 46-47 0.17156936811149137 2304 48-49 0.30181796143981643 2304 50-51 0.18699170664437048 2304 52-53 0.23315075403806418 2304 54-55 0.26003324035247743 2304 56-57 0.2318096849830411 2304 58-59 0.2747751381867616 2304 60-61 0.3064504335701983 2304 62-63 0.25114146947753824 2304 64-65 0.16973479717965745 2304 66-67 0.024044510724579027 2304 68-69 0.31929653319409823 2304 70-71 0.19661362195611076 2304 72-73 0.11355033295325967 2304 74-75 0.10004323830588646 2304 76-77 0.006885097776379467 2304 78-79 0.3018726603557198 2304 80-81 0.3165403026507576 2304 82-83 0.29825609387145136 2304 84-85 0.28913553815350923 2304 86-87 0.2938037548453991 2304 88-89 0.22013559981291309 2304 90-91 0.1595624326497571 2304 92-93 0.3199258342179192 2304 94-95 0.10147399795214795 2304 96-97 0.2360193595950193 2304 98-99 0.44234137327528344 2304 100-101 0.5236203495550527 2202 1 0.05571084077636357 2202 2 0.10356757089928692 2202 3 0.07882494018132746 2202 4 0.09333947711042612 2202 5 0.0932978170117309 2202 6 0.07777640467188718 2202 7 0.05627136577393088 2202 8 0.01124320102620402 2202 9 0.0561391138254379 2202 10-11 0.06387672784953935 2202 12-13 0.06840238733937554 2202 14-15 0.09885277308585927 2202 16-17 0.1550513174585717 2202 18-19 0.1182645642131348 2202 20-21 0.14287702725215468 2202 22-23 0.21746462253872068 2202 24-25 0.16273907249830444 2202 26-27 0.18327714061118883 2202 28-29 0.14283848251231035 2202 30-31 0.1286050903800131 2202 32-33 0.12604390402722032 2202 34-35 0.18229861568755013 2202 36-37 0.1849723463390589 2202 38-39 0.2918495951824198 2202 40-41 0.32134341608328043 2202 42-43 0.2957353456459515 2202 44-45 0.1448479369736546 2202 46-47 0.31141559408911235 2202 48-49 0.45021168647587473 2202 50-51 0.23391097400158856 2202 52-53 0.26287254142408045 2202 54-55 0.19214977246900844 2202 56-57 0.23109641127706482 2202 58-59 0.27088953868071997 2202 60-61 0.2805018999574216 2202 62-63 0.4452335186790819 2202 64-65 0.1662278681252758 2202 66-67 0.14312970645211465 2202 68-69 0.21590694586290482 2202 70-71 0.14852862350614515 2202 72-73 0.14051100297962904 2202 74-75 0.14334118123885986 2202 76-77 0.21797094426368702 2202 78-79 0.31882482438818016 2202 80-81 0.3268294808231431 2202 82-83 0.30923779317431865 2202 84-85 0.3920287290029094 2202 86-87 0.2798516189954583 2202 88-89 0.22420284840570304 2202 90-91 0.15790286712157098 2202 92-93 0.3453436399276981 2202 94-95 0.22276165683105376 2202 96-97 0.11756404296451706 2202 98-99 0.2588167404805759 2202 100-101 0.43087734586314497 1102 1 0.051431305312995335 1102 2 0.07790005370145536 1102 3 0.07621588261558543 1102 4 0.11572752269385234 1102 5 0.09883257567449988 1102 6 0.11648613548941711 1102 7 0.0796937644610054 1102 8 0.13921188193040024 1102 9 0.12851852797578545 1102 10-11 0.07866769345859126 1102 12-13 0.051638843674965074 1102 14-15 0.12113866033806886 1102 16-17 0.07267244720674171 1102 18-19 0.13582610160143105 1102 20-21 -0.00666065900352919 1102 22-23 0.08989085925755802 1102 24-25 0.07262537405929947 1102 26-27 0.0669144265102588 1102 28-29 0.14954628840202844 1102 30-31 0.10015434985034943 1102 32-33 0.07060042572522462 1102 34-35 0.11527849380698285 1102 36-37 0.019689625522168797 1102 38-39 0.14260679651710007 1102 40-41 0.09572390384202123 1102 42-43 0.06424669538925087 1102 44-45 0.11873442298718118 1102 46-47 0.28253623787577453 1102 48-49 0.1773886403130831 1102 50-51 -0.06843381982539398 1102 52-53 0.08757156453503967 1102 54-55 0.1141855846395643 1102 56-57 0.2768244430974889 1102 58-59 0.2812238470900965 1102 60-61 0.08326845161715113 1102 62-63 0.19055918383150328 1102 64-65 0.07779540731553425 1102 66-67 0.2135846657424878 1102 68-69 0.005621115365322282 1102 70-71 -0.014044536757097603 1102 72-73 -0.031743078032874905 1102 74-75 -0.006737565808123236 1102 76-77 0.049863025337668176 1102 78-79 0.2262251549838865 1102 80-81 0.21461848603274092 1102 82-83 0.016324672686607755 1102 84-85 -0.06650917717696103 1102 86-87 0.005441689773206804 1102 88-89 0.037301580176240634 1102 90-91 -0.07588895690124531 1102 92-93 -0.05819276461929945 1102 94-95 -0.20176686591691606 1102 96-97 -0.1951873031843263 1102 98-99 0.09574898727807835 1102 100-101 0.09748895265762059 2305 1 0.012620731639742644 2305 2 -0.024737097405377995 2305 3 0.04808955363198919 2305 4 0.03990595902574512 2305 5 0.025884526342622394 2305 6 0.0191443265049287 2305 7 -0.013222948836663306 2305 8 0.018194803791757863 2305 9 -0.0032929186298531476 2305 10-11 0.015170368212942265 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-0.17552709286614743 1213 96-97 -0.28200684674720833 1213 98-99 -0.17882557533075527 1213 100-101 -0.06410223679249327 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 1.0 9 7.0 10 15.0 11 7.0 12 14.0 13 51.0 14 121.0 15 297.0 16 643.0 17 1092.0 18 1764.0 19 2563.0 20 3617.0 21 4824.0 22 6433.0 23 8334.0 24 10285.0 25 13064.0 26 16900.0 27 22412.0 28 30627.0 29 38281.0 30 39740.0 31 47034.0 32 63118.0 33 90646.0 34 138577.0 35 236796.0 36 459047.0 37 772255.0 38 280279.0 39 493.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.32489074751241 25.75540135107633 24.140806050301006 14.778901851110252 2 29.106536474736366 24.170742808619785 33.52484429996794 13.197876416675914 3 36.4650392384174 20.003817697517796 27.3941200469306 16.13702301713421 4 28.17424981889393 20.39975287432156 34.17316449324817 17.252832813536333 5 31.14830575476404 18.94504000719859 33.82091242097061 16.08574181706677 6 25.452860171805124 19.813631713622016 38.460856369832676 16.272651744740184 7 31.51810698114477 21.22246693148849 29.52089206574128 17.738534021625465 8 28.05439825836115 28.879047130655238 26.116545481706936 16.950009129276673 9 29.90991282195988 25.64374504769501 26.323723277209393 18.122618853135712 10-11 23.939125633698474 29.287090853338388 30.028025568963606 16.745757943999532 12-13 23.167286787708935 27.712487190620173 29.864681405716414 19.25554461595448 14-15 22.15186224139904 28.37678403101683 30.649952082217652 18.82140164536648 16-17 21.9938471296069 28.401900462054968 31.384509408396667 18.219742999941467 18-19 20.65297915816712 28.408496255249332 32.367784922977734 18.570739663605814 20-21 21.44737911134136 28.870791468974872 31.93781346397954 17.744015955704224 22-23 20.561467987689017 30.642875800777343 30.938659996907404 17.856996214626236 24-25 20.7286778972786 28.676979982859674 31.73775563066703 18.856586489194694 26-27 20.66023016260596 28.859281591446962 30.792722612388385 19.687765633558698 28-29 21.202068021410554 29.776249728087333 30.681161977829397 18.340520272672713 30-31 20.064970747001972 29.313233781992874 31.392699548952578 19.229095922052576 32-33 19.87596414334624 30.339840600208447 30.849136300537534 18.935058955907778 34-35 20.14580197419516 29.30958643939864 31.748828700698628 18.79578288570757 36-37 20.893944013509582 29.249634610529334 31.07431056488819 18.782110811072894 38-39 21.49311285620559 29.96211131776959 30.332021746024395 18.21275408000042 40-41 21.370915959111326 29.965649458489747 30.00635991714635 18.657074665252576 42-43 20.133877129545745 30.013763804208903 31.266047215395893 18.58631185084946 44-45 19.84923152457173 29.181820246726346 31.62064754090484 19.348300687797085 46-47 20.982411279425285 29.119747140710857 30.4862347671476 19.41160681271626 48-49 20.26564554777354 30.391391779023163 30.7012744547513 18.641688218452 50-51 21.187999489808398 29.478787735832572 30.42901959345417 18.90419318090486 52-53 20.110541450422996 29.309471942451697 29.82542086431655 20.754565742808754 54-55 21.022736084193255 29.371728674996376 30.506992987899906 19.09854225291046 56-57 20.206652793119815 29.413455651213177 30.54457858063874 19.83531297502827 58-59 20.175625990602057 29.333834859756085 30.98028756511543 19.510251584526426 60-61 20.680459318230216 29.60064649641533 30.885405234218442 18.833488951136008 62-63 19.837648070184326 29.60840192094828 30.469936914828466 20.084013094038927 64-65 20.630922750460606 29.431055553310625 30.435813958060077 19.502207738168696 66-67 20.029253785986448 30.61044341810567 29.798974151510233 19.561328644397644 68-69 19.865996283985638 31.101292521985325 29.692593203473088 19.34011799055595 70-71 19.529132558224376 31.792312217610714 29.3941536748208 19.28440154934411 72-73 19.56239189879346 31.410830239110364 29.535881845900054 19.49089601619612 74-75 19.852156357136835 30.833169858753596 29.712884753261154 19.60178903084842 76-77 19.947255787151565 30.579061381935073 29.824399298986464 19.649283531926894 78-79 19.889536671710694 30.56206687471824 29.960562228504926 19.587834225066135 80-81 19.572732681415626 30.955214074852595 29.78024205349709 19.69181119023469 82-83 19.38779254294503 30.68546058614734 29.805724140160272 20.121022730747356 84-85 19.534385543726486 30.343894751004246 30.130836124608827 19.990883580660434 86-87 19.67165093940868 30.422550963502527 30.294829566094567 19.610968530994228 88-89 19.94263416270823 30.855140229786638 29.63069349709676 19.571532110408377 90-91 20.12981707911135 30.51835085038182 29.45260479537667 19.89922727513016 92-93 19.721298866465975 30.73502224973661 29.688909995550272 19.854768888247147 94-95 19.33666356623556 31.062693512992322 29.655135655147014 19.945507265625103 96-97 19.5884179807945 31.402428834595852 29.316885081779244 19.692268102830408 98-99 20.009013915641898 30.786345075673417 29.04444847878704 20.160192529897646 100-101 19.97928315432543 30.646907753023918 28.807403017573275 20.566406075077374 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 26.0 1 24.0 2 28.5 3 55.5 4 68.0 5 50.0 6 47.5 7 49.0 8 40.0 9 45.5 10 52.5 11 54.5 12 60.5 13 72.0 14 82.5 15 95.5 16 125.5 17 162.0 18 258.5 19 438.5 20 758.0 21 1276.0 22 2036.5 23 3185.0 24 4808.0 25 7137.5 26 11294.0 27 16748.5 28 22374.0 29 28303.5 30 34497.5 31 40255.0 32 47194.5 33 57422.0 34 69718.5 35 85879.0 36 106559.5 37 128130.5 38 148887.5 39 160200.5 40 162013.5 41 152329.5 42 134559.0 43 123420.0 44 111965.5 45 101262.0 46 97152.0 47 87505.0 48 70366.0 49 56798.5 50 46744.5 51 37567.5 52 29909.5 53 24016.5 54 19652.0 55 15525.0 56 11511.5 57 8258.5 58 5935.0 59 4243.0 60 3048.0 61 2168.0 62 1515.5 63 1007.0 64 692.0 65 483.0 66 334.5 67 254.5 68 185.5 69 116.0 70 83.5 71 62.5 72 46.0 73 33.5 74 23.0 75 16.5 76 14.5 77 8.5 78 5.0 79 3.5 80 2.5 81 1.5 82 2.0 83 2.5 84 2.0 85 0.5 86 0.5 87 1.5 88 1.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1962576080945671 2 0.0 3 0.0 4 0.027256787770088997 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 6.552112444732931E-5 48-49 0.0036910233438662184 50-51 8.736149926310574E-5 52-53 0.0026645257275247254 54-55 0.009762647542652068 56-57 0.014392807003596672 58-59 0.014239924379886236 60-61 0.0032978965971822423 62-63 0.0022713989808407498 64-65 0.010068412790072938 66-67 0.017625182476331585 68-69 0.02601188640558974 70-71 0.02177485369132911 72-73 0.017253896104463387 74-75 0.02188405556540799 76-77 0.01600899473996413 78-79 0.007076281440311566 80-81 0.004018628966102865 82-83 6.98891994104846E-4 84-85 0.0027082064771562784 86-87 0.016227398488121894 88-89 0.015069858622885741 90-91 0.018564318593409974 92-93 0.018979285714909724 94-95 0.017865426599305127 96-97 0.020202346704593206 98-99 0.028589050633851355 100-101 0.016576844485174318 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2289338.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.41870518862052 #Duplication Level Percentage of deduplicated Percentage of total 1 92.46792153427673 61.415996197896206 2 3.9061583267213513 5.188839566451613 3 1.0611883196144452 2.114482624502383 4 0.5815189653541202 1.5449494688578775 5 0.34956619257435634 1.160886694425236 6 0.2682675574961824 1.0690790287806147 7 0.17214806819961306 0.8003696253378447 8 0.15313100776733754 0.8136610608108121 9 0.14427684346536163 0.8624413018503545 >10 0.766249798091473 9.749819945944127 >50 0.06422465242886972 2.895706315902378 >100 0.06127208997223472 8.626366228731305 >500 0.0032853396013767695 1.393694673488534 >1k 7.255251083766483E-4 0.9122017695531793 >5k 0.0 0.0 >10k+ 6.577932822602539E-5 1.4515054974674204 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT 33223 1.4512055450090813 TruSeq Adapter, Index 6 (97% over 36bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 2615 0.11422516028651078 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 2486 0.10859034358404046 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 2411 0.10531428736167399 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 7.862534933679517E-4 0.0 0.0 2 7.862534933679517E-4 0.0 0.0 3 7.862534933679517E-4 0.0 0.0 4 8.736149926310575E-4 0.0 0.0 5 0.0012230609896834806 0.0 0.0 6 0.0013541032385781392 0.0 0.0 7 0.001703549235630562 0.0 0.0 8 0.0019219529837883266 0.0 0.0 9 0.002184037481577644 0.0 0.0 10-11 0.0034070984712611246 0.0 0.0 12-13 0.004368074963155288 0.0 0.0 14-15 0.005416412954312557 0.0 0.0 16-17 0.0066613143188118135 0.0 0.0 18-19 0.00827750205517927 0.0 4.368074963155288E-5 20-21 0.01000289166562561 0.0 4.368074963155288E-5 22-23 0.012536375144255676 0.0 4.368074963155288E-5 24-25 0.015550346868832825 0.0 4.368074963155288E-5 26-27 0.018302234095620654 0.0 6.552112444732931E-5 28-29 0.021469088443908237 0.0 1.3104224889465862E-4 30-31 0.03295712559700664 0.0 1.3104224889465862E-4 32-33 0.03752176393350393 0.0 1.3104224889465862E-4 34-35 0.04213008301963275 0.0 1.3104224889465862E-4 36-37 0.04614871198573561 0.0 1.3104224889465862E-4 38-39 0.050254702451101585 0.0 1.3104224889465862E-4 40-41 0.054906702286861966 0.0 1.3104224889465862E-4 42-43 0.0596023828722539 0.0 1.3104224889465862E-4 44-45 0.06447278645617205 0.0 1.5288262371043507E-4 46-47 0.06991103978530039 0.0 2.1840374815776438E-4 48-49 0.07615738698261244 0.0 2.6208449778931725E-4 50-51 0.08301526467476625 0.0 2.6208449778931725E-4 52-53 0.09039731136249868 0.0 2.6208449778931725E-4 54-55 0.09867481341767795 0.0 2.6208449778931725E-4 56-57 0.10647182722691013 0.0 3.0576524742087014E-4 58-59 0.11455276590874741 0.0 4.1496712149975234E-4 60-61 0.12370388295655775 0.0 4.3680749631552876E-4 62-63 0.13462407036444596 0.0 4.586478711313052E-4 64-65 0.14578450189530773 0.0 5.241689955786345E-4 66-67 0.15856112116253696 0.0 5.241689955786345E-4 68-69 0.17358729903579112 0.0 5.241689955786345E-4 70-71 0.1912343218869385 0.0 5.241689955786345E-4 72-73 0.20960207710700646 0.0 5.241689955786345E-4 74-75 0.22993546606049436 0.0 5.46009370394411E-4 76-77 0.2518850427503497 0.0 5.678497452101874E-4 78-79 0.27617153954549306 0.0 5.678497452101874E-4 80-81 0.30491347280305486 0.0 5.678497452101874E-4 82-83 0.33684410078372 0.0 6.115304948417403E-4 84-85 0.3734048882253298 0.0 6.115304948417403E-4 86-87 0.4140498257574897 0.0 6.333708696575166E-4 88-89 0.46188024660404015 0.0 6.552112444732931E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 5390 0.0 67.95403 3 CGGAAGA 5385 0.0 67.75247 4 GATCGGA 5575 0.0 65.934074 1 ATCGGAA 5740 0.0 63.810493 2 GAAGAGC 6035 0.0 60.280632 6 GGAAGAG 7090 0.0 51.444775 5 GCGCGGA 40 5.325816E-4 47.614315 1 ATGCCGT 3975 0.0 43.79455 48-49 GCCGTCT 3990 0.0 43.74991 50-51 TATGCCG 3965 0.0 43.725307 48-49 CGTATGC 3985 0.0 43.684654 46-47 TCGTATG 4145 0.0 43.25892 44-45 TGCCGTC 3965 0.0 43.187172 50-51 AATCTCG 4065 0.0 42.93662 40-41 TTGTGTT 24655 0.0 42.873158 1 CGTCTTC 4080 0.0 42.67028 52-53 TCTCGTA 4065 0.0 42.64966 42-43 CACTCCG 4280 0.0 42.33281 30-31 CTCGTAT 4025 0.0 42.306442 44-45 GTATGCC 4205 0.0 42.189835 46-47 >>END_MODULE