FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n01_30245-1.34100000035771.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n01_30245-1.34100000035771.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2289338
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT332231.4512055450090813TruSeq Adapter, Index 6 (97% over 36bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC26150.11422516028651078No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC24860.10859034358404046No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG24110.10531428736167399No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG53900.067.954033
CGGAAGA53850.067.752474
GATCGGA55750.065.9340741
ATCGGAA57400.063.8104932
GAAGAGC60350.060.2806326
GGAAGAG70900.051.4447755
GCGCGGA405.325816E-447.6143151
ATGCCGT39750.043.7945548-49
GCCGTCT39900.043.7499150-51
TATGCCG39650.043.72530748-49
CGTATGC39850.043.68465446-47
TCGTATG41450.043.2589244-45
TGCCGTC39650.043.18717250-51
AATCTCG40650.042.9366240-41
TTGTGTT246550.042.8731581
CGTCTTC40800.042.6702852-53
TCTCGTA40650.042.6496642-43
CACTCCG42800.042.3328130-31
CTCGTAT40250.042.30644244-45
GTATGCC42050.042.18983546-47
Produced by FastQC (version 0.11.2)