##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n01_30230-1.34100000035714.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2276208 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.68904379564609 33.0 33.0 33.0 33.0 33.0 2 32.71404722239795 33.0 33.0 33.0 33.0 33.0 3 32.74145684401426 33.0 33.0 33.0 33.0 33.0 4 36.673149817591366 37.0 37.0 37.0 37.0 37.0 5 36.668256591664736 37.0 37.0 37.0 37.0 37.0 6 36.54992601730598 37.0 37.0 37.0 37.0 37.0 7 36.47983268664375 37.0 37.0 37.0 37.0 37.0 8 36.39374433267962 37.0 37.0 37.0 37.0 37.0 9 36.4067027266401 37.0 37.0 37.0 37.0 37.0 10-11 36.34586997321861 37.0 37.0 37.0 37.0 37.0 12-13 36.313034441492164 37.0 37.0 37.0 37.0 37.0 14-15 38.10551386340791 40.0 37.0 40.0 35.0 40.0 16-17 38.04473448823658 40.0 37.0 40.0 35.0 40.0 18-19 38.076952984964464 40.0 37.0 40.0 37.0 40.0 20-21 38.01215881852625 40.0 37.0 40.0 33.0 40.0 22-23 37.872119551464536 40.0 37.0 40.0 33.0 40.0 24-25 37.869020757329736 40.0 37.0 40.0 33.0 40.0 26-27 37.746004978455396 40.0 37.0 40.0 33.0 40.0 28-29 37.60245746434421 40.0 37.0 40.0 33.0 40.0 30-31 37.56311571701707 40.0 37.0 40.0 33.0 40.0 32-33 37.48008815538826 40.0 37.0 40.0 33.0 40.0 34-35 37.33709485249152 40.0 37.0 40.0 33.0 40.0 36-37 37.21411092483639 40.0 37.0 40.0 33.0 40.0 38-39 37.11324690010755 38.5 37.0 40.0 33.0 40.0 40-41 37.01102908873003 37.0 37.0 40.0 33.0 40.0 42-43 36.76779033374805 37.0 37.0 40.0 33.0 40.0 44-45 36.605983943470896 37.0 37.0 40.0 33.0 40.0 46-47 36.58800557769764 37.0 37.0 40.0 33.0 40.0 48-49 36.355917165742326 37.0 37.0 40.0 33.0 40.0 50-51 36.21911837582506 37.0 37.0 40.0 33.0 40.0 52-53 36.08420913203011 37.0 37.0 40.0 33.0 40.0 54-55 35.86444714191322 37.0 37.0 40.0 33.0 40.0 56-57 35.65915680816516 37.0 33.0 40.0 27.0 40.0 58-59 35.41645666828339 37.0 33.0 40.0 27.0 40.0 60-61 35.19855171407885 37.0 33.0 40.0 27.0 40.0 62-63 34.90730306720651 37.0 33.0 37.0 27.0 40.0 64-65 34.62999493016456 37.0 33.0 37.0 27.0 40.0 66-67 34.15210890217414 37.0 33.0 37.0 27.0 40.0 68-69 33.85880398452163 37.0 33.0 37.0 27.0 38.5 70-71 33.49587230165257 37.0 33.0 37.0 27.0 37.0 72-73 32.967873542312475 37.0 33.0 37.0 27.0 37.0 74-75 32.47648259737247 35.0 33.0 37.0 22.0 37.0 76-77 29.79821527733845 33.0 27.0 33.0 22.0 37.0 78-79 32.16378424115898 33.0 33.0 37.0 22.0 37.0 80-81 32.66305385975271 35.0 33.0 37.0 27.0 37.0 82-83 32.46454322276347 35.0 33.0 37.0 22.0 37.0 84-85 32.326012605174924 33.0 33.0 37.0 22.0 37.0 86-87 32.25572882618812 33.0 33.0 37.0 22.0 37.0 88-89 32.175009050139536 33.0 33.0 37.0 22.0 37.0 90-91 32.07342540751988 33.0 33.0 37.0 22.0 37.0 92-93 31.909714753660474 33.0 33.0 37.0 22.0 37.0 94-95 31.70828083373751 33.0 33.0 37.0 22.0 37.0 96-97 31.52403976262275 33.0 33.0 37.0 15.0 37.0 98-99 31.33443143157392 33.0 33.0 37.0 15.0 37.0 100-101 29.539571076105524 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.07534968543373566 2306 2 0.08641768005981731 2306 3 0.09620558923143818 2306 4 0.1339298302793921 2306 5 0.1779636269965792 2306 6 0.17397657023036572 2306 7 0.2205669675038493 2306 8 0.2584856373645934 2306 9 0.23617977882311436 2306 10-11 0.2769014113319841 2306 12-13 0.28016658224767355 2306 14-15 0.4550378863263589 2306 16-17 0.4666889931915321 2306 18-19 0.28618579133534894 2306 20-21 0.45895319950309243 2306 22-23 0.3953071866358613 2306 24-25 0.32161670358359373 2306 26-27 0.4030511603354512 2306 28-29 0.32925837456554774 2306 30-31 0.3436937212655238 2306 32-33 0.5012785404274567 2306 34-35 0.42607267210750166 2306 36-37 0.393787211569375 2306 38-39 0.49836419490134887 2306 40-41 0.5261252465278332 2306 42-43 0.5432690700223404 2306 44-45 0.4755438839975952 2306 46-47 0.5087033385653115 2306 48-49 0.44129546489487836 2306 50-51 0.5922415070526554 2306 52-53 0.4971320585229435 2306 54-55 0.5181822541559171 2306 56-57 0.5176562712674695 2306 58-59 0.6020824089651811 2306 60-61 0.6973424193613198 2306 62-63 0.5354464108583556 2306 64-65 0.5265246545127908 2306 66-67 0.5852551622226017 2306 68-69 0.6428647033497725 2306 70-71 0.5836884437313756 2306 72-73 0.21444796940016175 2306 74-75 0.21866288227818842 2306 76-77 0.31442866463516594 2306 78-79 0.37575518585691015 2306 80-81 0.532575643625961 2306 82-83 0.5606924774521715 2306 84-85 0.5322524420339789 2306 86-87 0.3722785586685191 2306 88-89 0.5057459338676651 2306 90-91 0.39998384010488053 2306 92-93 0.4862132485574371 2306 94-95 0.4587433272880652 2306 96-97 0.519342818823123 2306 98-99 0.550736595928413 2306 100-101 0.5312695741652433 2307 1 0.06595977315876667 2307 2 0.043791616181351856 2307 3 0.09424419608993873 2307 4 0.10098751177608278 2307 5 0.10028121514650934 2307 6 0.20076081371635723 2307 7 0.18798254359975886 2307 8 0.16893910126136547 2307 9 0.24618677329290506 2307 10-11 0.23563371270865474 2307 12-13 0.19695527081726993 2307 14-15 0.509474145134817 2307 16-17 0.4743639529896768 2307 18-19 0.39531864050320564 2307 20-21 0.5624561541763171 2307 22-23 0.5825532285503812 2307 24-25 0.5603503777066692 2307 26-27 0.4630619135156451 2307 28-29 0.5300228635770381 2307 30-31 0.5769404953114687 2307 32-33 0.5449907081901415 2307 34-35 0.5004266641848005 2307 36-37 0.45949845763089314 2307 38-39 0.5453054217444304 2307 40-41 0.5646538258006757 2307 42-43 0.4574877812095721 2307 44-45 0.5719977858658396 2307 46-47 0.6371175549885919 2307 48-49 0.6065837743249887 2307 50-51 0.6096314258645137 2307 52-53 0.5873894811588727 2307 54-55 0.4912507672754529 2307 56-57 0.6690458501611687 2307 58-59 0.6072864252289563 2307 60-61 0.6507437254314752 2307 62-63 0.46554011714292187 2307 64-65 0.557314866177208 2307 66-67 0.8529433265338113 2307 68-69 0.6823888506127744 2307 70-71 0.4753043950446383 2307 72-73 0.5014990486403121 2307 74-75 0.4830540038773705 2307 76-77 0.3976631820167533 2307 78-79 0.6180313628235226 2307 80-81 0.5349184641622458 2307 82-83 0.39919605312972095 2307 84-85 0.44359263474142097 2307 86-87 0.44998172513690093 2307 88-89 0.5005778704860049 2307 90-91 0.4841014910089285 2307 92-93 0.5469641408426078 2307 94-95 0.31007276681531337 2307 96-97 0.4623254699232824 2307 98-99 0.5058920758013343 2307 100-101 0.2897497151005375 2201 1 0.08989637326701683 2201 2 0.12288933043488015 2201 3 0.11513166477205061 2201 4 0.17519804389237237 2201 5 0.1270774474486842 2201 6 0.17938138176096885 2201 7 0.19146945192743914 2201 8 0.22757579663966965 2201 9 0.2533390682235108 2201 10-11 0.21936096361665847 2201 12-13 0.27404747704142096 2201 14-15 0.4603589442962388 2201 16-17 0.4592707524303066 2201 18-19 0.4492501526029926 2201 20-21 0.45534240198130504 2201 22-23 0.3701363755479079 2201 24-25 0.33377052352617653 2201 26-27 0.4426815619226261 2201 28-29 0.5363124624876789 2201 30-31 0.6143387351421836 2201 32-33 0.5088848286320555 2201 34-35 0.41983637560716147 2201 36-37 0.5277960932682646 2201 38-39 0.6208181105395454 2201 40-41 0.5618684319973895 2201 42-43 0.5251873768629096 2201 44-45 0.6742280295759358 2201 46-47 0.6165564290876233 2201 48-49 0.5648671561913545 2201 50-51 0.518865426496383 2201 52-53 0.5448547100396581 2201 54-55 0.6343078399133546 2201 56-57 0.5144954501004264 2201 58-59 0.525648686533394 2201 60-61 0.5557802721493132 2201 62-63 0.5801104651357534 2201 64-65 0.6924579733033625 2201 66-67 0.7209453250006774 2201 68-69 0.6524839431030998 2201 70-71 0.6169645613876185 2201 72-73 0.4843804411014929 2201 74-75 0.4497883167696841 2201 76-77 0.23076395274900818 2201 78-79 0.47876060117743435 2201 80-81 0.47216146156865335 2201 82-83 0.4705285847904861 2201 84-85 0.5854813328725115 2201 86-87 0.6964239459331978 2201 88-89 0.5502848354858969 2201 90-91 0.5187210720656168 2201 92-93 0.5463889081167359 2201 94-95 0.5706487526399151 2201 96-97 0.7226221051026691 2201 98-99 0.7888791776080346 2201 100-101 0.8024796857358147 1101 1 0.006977018327269491 1101 2 -0.01206288004743783 1101 3 0.07477382570225188 1101 4 0.10993800205697113 1101 5 0.13713878819766023 1101 6 0.11001325810676121 1101 7 0.14210726245394056 1101 8 0.16018383489178234 1101 9 0.21336051555993407 1101 10-11 0.19178724053331564 1101 12-13 0.15589278513758842 1101 14-15 0.3019096277236457 1101 16-17 0.3951848753817444 1101 18-19 0.30378171395883413 1101 20-21 0.3635700410259375 1101 22-23 0.43413483448331647 1101 24-25 0.3258887982400154 1101 26-27 0.41373192899327904 1101 28-29 0.48668958479728985 1101 30-31 0.5231589380091535 1101 32-33 0.42026834698615545 1101 34-35 0.47808901566265405 1101 36-37 0.44326477110205076 1101 38-39 0.5418980630888726 1101 40-41 0.6283958940986523 1101 42-43 0.5891172111557452 1101 44-45 0.49659897965421607 1101 46-47 0.5600397646894635 1101 48-49 0.6199083907742988 1101 50-51 0.6105599084486713 1101 52-53 0.4458626665117009 1101 54-55 0.5336777745747199 1101 56-57 0.5430717763641866 1101 58-59 0.4152796169411701 1101 60-61 0.4937576903706642 1101 62-63 0.5242314973642834 1101 64-65 0.4596278019240003 1101 66-67 0.6071061549658978 1101 68-69 0.7407909019835515 1101 70-71 0.4728959515520259 1101 72-73 0.43645324700651145 1101 74-75 0.4692278840870401 1101 76-77 0.23526669744996553 1101 78-79 0.6495065424996653 1101 80-81 0.6486042448474691 1101 82-83 0.5687567876392379 1101 84-85 0.8232762551045596 1101 86-87 0.698734688565736 1101 88-89 0.443096703163917 1101 90-91 0.48175588155962146 1101 92-93 0.8057872095286918 1101 94-95 0.47500920798861657 1101 96-97 0.5627383626506663 1101 98-99 0.6598702598214992 1101 100-101 0.5924791674916214 2304 1 0.06693214675869541 2304 2 0.07811416663827941 2304 3 0.08871557380709305 2304 4 0.12826863849451797 2304 5 0.14715058568967976 2304 6 0.15782682295687778 2304 7 0.08726525874556046 2304 8 0.11251622382859239 2304 9 0.053479207076328805 2304 10-11 0.1330716654727624 2304 12-13 0.07459481336832141 2304 14-15 0.18511801337465528 2304 16-17 0.16052662064127787 2304 18-19 0.18728833009141255 2304 20-21 0.3351123457574232 2304 22-23 0.2594706994386584 2304 24-25 0.15616812322745943 2304 26-27 0.2975237346102375 2304 28-29 0.300476131862645 2304 30-31 0.288909301181171 2304 32-33 0.2498661152104944 2304 34-35 0.27855596239900393 2304 36-37 0.3761265560272875 2304 38-39 0.39693828995481084 2304 40-41 0.41636798478389636 2304 42-43 0.43815502267091944 2304 44-45 0.4842766862934198 2304 46-47 0.4909847864797854 2304 48-49 0.46902010127364235 2304 50-51 0.36929355648717177 2304 52-53 0.34119456911152923 2304 54-55 0.4132757799080977 2304 56-57 0.3010548425634525 2304 58-59 0.46247498583278457 2304 60-61 0.444807714582133 2304 62-63 0.5203041812963534 2304 64-65 0.4221420482254885 2304 66-67 0.425301744632371 2304 68-69 0.5263472467316674 2304 70-71 0.3880268541839911 2304 72-73 0.23057871246473383 2304 74-75 0.25220619493277496 2304 76-77 0.2027814416804432 2304 78-79 0.26790364850479165 2304 80-81 0.27523891408826984 2304 82-83 0.27289568400730246 2304 84-85 0.14766249835420098 2304 86-87 0.16374714205942809 2304 88-89 0.23243388328406667 2304 90-91 0.16612172816272164 2304 92-93 0.3449452405962994 2304 94-95 0.08468333586204935 2304 96-97 0.24308850420476702 2304 98-99 0.30943706160519113 2304 100-101 0.3190700505963413 2202 1 0.06760193614550047 2202 2 0.02050709295107822 2202 3 0.04115855156425141 2202 4 0.08684912954497293 2202 5 0.12574597412059774 2202 6 0.10081297718527082 2202 7 0.15043909230684704 2202 8 0.07262572155076441 2202 9 0.15632917079607012 2202 10-11 0.06431452488070732 2202 12-13 0.14012229473794946 2202 14-15 0.10834747995853178 2202 16-17 0.07662846122843803 2202 18-19 0.15828252278034682 2202 20-21 0.07680357383799219 2202 22-23 0.103709843886449 2202 24-25 0.05327543326734485 2202 26-27 0.05364964019661045 2202 28-29 0.1406877724663005 2202 30-31 0.09033724671089516 2202 32-33 0.15133952837520326 2202 34-35 0.21828887153239407 2202 36-37 0.11623790110366627 2202 38-39 0.19479896487134596 2202 40-41 0.14697101256781053 2202 42-43 0.16702986047174306 2202 44-45 0.1960654734461187 2202 46-47 0.1054145492330747 2202 48-49 0.28194535702600376 2202 50-51 0.18637227205241658 2202 52-53 0.142402215182166 2202 54-55 0.21846239972902026 2202 56-57 0.328716749082389 2202 58-59 0.24122270689415615 2202 60-61 -0.0027738567259874003 2202 62-63 0.20718875672841364 2202 64-65 0.19602996815898166 2202 66-67 0.15325586899881927 2202 68-69 0.07960352958827599 2202 70-71 0.04249307995537066 2202 72-73 -0.16701525686227114 2202 74-75 -0.04131406199763887 2202 76-77 -0.1069356414559337 2202 78-79 0.02848197410047959 2202 80-81 0.10144770858097729 2202 82-83 0.03942592307173243 2202 84-85 -0.022941650684806802 2202 86-87 -0.06377885549949269 2202 88-89 -0.02045408950154126 2202 90-91 -0.07224327445271683 2202 92-93 0.0036679137079609347 2202 94-95 -0.19304142515501965 2202 96-97 -0.06703620702265312 2202 98-99 0.09833127686739829 2202 100-101 0.26169130297406795 1102 1 0.07864430986456483 1102 2 0.09627016037779157 1102 3 0.1205831893886753 1102 4 0.12485971748307634 1102 5 0.08875134497301218 1102 6 0.07121562636645962 1102 7 0.09188279720651593 1102 8 0.13430540347328446 1102 9 0.15585826016749849 1102 10-11 0.16884570108640418 1102 12-13 0.13501161014647778 1102 14-15 0.16284955031779447 1102 16-17 0.17158834458538053 1102 18-19 0.279039521912658 1102 20-21 0.08732767128217489 1102 22-23 0.173141016797139 1102 24-25 0.11055422883445942 1102 26-27 0.1221245762186598 1102 28-29 0.2637389163809871 1102 30-31 0.19101944930955028 1102 32-33 0.2148033163089167 1102 34-35 0.24024250536986358 1102 36-37 0.12090719278818085 1102 38-39 0.20464415471834485 1102 40-41 0.1067067809072384 1102 42-43 0.18002246319341708 1102 44-45 0.19665033620930217 1102 46-47 0.10389884855101172 1102 48-49 0.1331012160878089 1102 50-51 0.1694442019347875 1102 52-53 0.08751229569003982 1102 54-55 0.21714028038406497 1102 56-57 0.18886727573345752 1102 58-59 0.2898507506517731 1102 60-61 -0.03681190850144134 1102 62-63 0.06025846248478217 1102 64-65 0.14614021341692762 1102 66-67 0.21782005013603367 1102 68-69 0.351460372757181 1102 70-71 0.23085939076187856 1102 72-73 0.3129168111904903 1102 74-75 0.20751278018277475 1102 76-77 0.11322262844862507 1102 78-79 0.1241804869564831 1102 80-81 0.2740645987585779 1102 82-83 0.3770894375203113 1102 84-85 0.404455737477349 1102 86-87 0.38205269304136635 1102 88-89 0.5085240756219633 1102 90-91 0.40754395082653616 1102 92-93 0.4684793572481105 1102 94-95 0.19637695216658102 1102 96-97 0.22018769931143822 1102 98-99 0.41580604264770926 1102 100-101 0.3186625650694701 2305 1 0.11979107384893695 2305 2 0.11424465990835841 2305 3 0.05836522397482469 2305 4 0.058269269559147574 2305 5 0.10461342438183863 2305 6 0.02783908558691195 2305 7 0.04794471105410025 2305 8 0.09710712369497543 2305 9 0.0687228229949568 2305 10-11 0.033792146062531 2305 12-13 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8396.0 23 10518.0 24 13084.0 25 16512.0 26 21140.0 27 28265.0 28 41101.0 29 59211.0 30 59833.0 31 60634.0 32 81154.0 33 115499.0 34 177546.0 35 306029.0 36 531071.0 37 616574.0 38 104229.0 39 44.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.542286148404486 21.860879271595994 23.726961177432713 12.869873402566808 2 35.38323386966393 20.814661928962554 31.47976810555099 12.322336095822525 3 41.799519200354275 15.33062883532612 27.85224373168006 15.017608232639548 4 35.868249736989895 14.396957608132965 31.59373135089195 18.141061303985182 5 35.546443910222614 13.356687965247463 34.98511559576278 16.11175252876714 6 29.763360817640567 13.97600746504713 39.54915368015577 16.711478037156535 7 36.79400125120375 17.27979165348685 27.638906461975353 18.287300633334034 8 33.673899749056325 27.548273268523793 21.064990545679482 17.712836436740403 9 34.52118611304415 25.568533279911147 21.405513028686308 18.50476757835839 10-11 30.10733201886647 26.83658523298398 26.584455374904227 16.471627373245326 12-13 28.249483351257883 25.216983685146527 26.354401706698155 20.17913125689744 14-15 26.94136476104117 25.75854227733142 26.09686812452992 21.20322483709749 16-17 27.108133351609343 26.24735964375839 26.770202898856343 19.874304105775924 18-19 24.605791737837666 26.36589011197571 27.891146151845525 21.1371719983411 20-21 25.00208680401791 27.23035416798465 28.455461891004692 19.312097136992755 22-23 24.311596304028456 28.260137034928267 27.320482135200297 20.10778452584298 24-25 25.11497191820783 24.12740839150025 29.459939513436385 21.297680176855543 26-27 22.512178148921365 24.174504263230777 29.51999553643604 23.79332205141182 28-29 23.076427989006277 27.413795224338024 27.06470586167872 22.445070924976978 30-31 20.216957325516823 27.058379550550743 29.035549475267636 23.689113648664797 32-33 20.99744399457343 26.484837940996602 28.231075543184105 24.28664252124586 34-35 21.841215741267934 26.739823425627186 28.904629981091357 22.514330852013526 36-37 20.30409786803315 29.428813184032393 29.566410451065984 20.700678496868477 38-39 20.200570422386708 27.51828918974013 27.277493972431344 25.00364641544182 40-41 23.14560005061049 26.622369308955946 25.48451196024265 24.747518680190915 42-43 23.291544533715726 28.391100461820713 26.954610474965378 21.362744529498183 44-45 20.761020082523213 28.507060866142286 26.66131566183758 24.070603389496917 46-47 22.426104508623894 28.139167636299817 26.668407869328085 22.766319985748204 48-49 21.427535833928278 27.633455983304962 28.92033609753419 22.018672085232577 50-51 22.037824327927996 26.71510104309494 26.781637170960288 24.46543745801678 52-53 20.46665774807256 27.898153170043983 26.287775792694966 25.34741328918849 54-55 22.31565201994217 27.787402443166265 28.618099184483526 21.27884635240804 56-57 21.980078426995863 27.217977089685746 26.519520298680792 24.2824241846376 58-59 20.48973723715312 26.41814230887613 27.315292348190706 25.77682810578004 60-61 20.873079194140225 26.21478229695342 29.786374052777347 23.125764456129005 62-63 20.287164288348745 26.25642339148041 29.61425931950547 23.842153000665377 64-65 23.149286454946484 28.097484138561313 27.594136100810207 21.159093305682 66-67 21.854662999960233 30.16490731694242 26.022945403438406 21.95748427965894 68-69 21.235398394976986 27.691854389556013 28.38485660522955 22.68789061023745 70-71 20.93650559639883 30.893971515194522 26.706477049195094 21.46304583921155 72-73 23.33662815772007 30.300337267276024 26.00433739206777 20.358697182936133 74-75 24.708326586338426 28.190786633326077 26.459938010405132 20.640948769930368 76-77 23.35013960800787 27.114052349542682 26.49854240407868 23.037265638370773 78-79 23.256440296207384 28.807374033985777 26.287098142585773 21.649087527221067 80-81 22.495718054334855 29.15198254578557 26.444883445625717 21.90741595425386 82-83 22.01438456999836 28.81056889388359 27.056606459305442 22.1184400768126 84-85 22.706940307638508 28.256871100727082 26.568007185832364 22.468181405802046 86-87 20.307702988490796 29.086891777113504 27.91907659224257 22.686328642153132 88-89 19.738462092777805 29.40447200733266 28.41257712516576 22.444488774723773 90-91 21.238987827014686 28.632858140318667 26.856102333400855 23.272051699265788 92-93 21.668922688076055 28.698393922210215 25.821027379187143 23.811656010526587 94-95 22.271013871529124 29.752064844680486 25.77100607199345 22.20591521179694 96-97 21.25314018874748 31.24015978985288 24.487606004027704 23.019094017371938 98-99 21.325373026760015 29.095614866006557 26.379337128915086 23.19967497831834 100-101 22.799170761886682 29.772271493753472 25.91935931776798 21.509198426591862 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 294.0 1 202.5 2 121.5 3 203.5 4 236.5 5 183.0 6 166.0 7 191.0 8 206.5 9 210.0 10 237.0 11 247.5 12 268.5 13 266.5 14 262.0 15 313.5 16 346.0 17 352.5 18 352.5 19 374.5 20 435.0 21 501.0 22 555.5 23 613.5 24 727.5 25 869.0 26 1096.5 27 1466.5 28 1942.5 29 2495.0 30 3194.5 31 4228.0 32 5852.0 33 8653.0 34 13710.0 35 23866.0 36 36939.0 37 51529.0 38 69411.0 39 84447.5 40 94922.0 41 101988.0 42 136562.0 43 175039.5 44 165586.5 45 141437.0 46 136293.0 47 132340.0 48 117140.0 49 105007.0 50 95477.0 51 90697.0 52 89486.0 53 89650.0 54 76387.5 55 48788.5 56 32081.0 57 26007.5 58 22496.0 59 18732.5 60 14774.5 61 11736.5 62 8956.5 63 6642.0 64 5075.0 65 3816.0 66 2953.0 67 2324.5 68 1823.5 69 1348.0 70 961.5 71 748.5 72 518.5 73 309.5 74 210.5 75 146.0 76 99.0 77 70.0 78 47.5 79 27.0 80 21.5 81 17.5 82 11.5 83 8.0 84 7.5 85 7.0 86 4.0 87 2.0 88 1.5 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18811989062510984 2 0.0 3 0.0 4 0.027677611184918075 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 1.5376450658287819E-4 48-49 0.00364641544182254 50-51 4.393271616653663E-5 52-53 0.0028336601927416126 54-55 0.009972726569803815 56-57 0.014651560841539964 58-59 0.014695493557706501 60-61 0.00351461729332293 62-63 0.002394333031076246 64-65 0.010895313609301082 66-67 0.01821011085102943 68-69 0.02644749513225505 70-71 0.02212012258985119 72-73 0.018297976283362504 74-75 0.02212012258985119 76-77 0.016321004055868355 78-79 0.0073587299578948846 80-81 0.003580516367572735 82-83 8.34721607164196E-4 84-85 0.0027457947604085393 86-87 0.016562633994784307 88-89 0.01590364325228626 90-91 0.018935000667777285 92-93 0.019615957768358602 94-95 0.018956967025860555 96-97 0.020604443882105677 98-99 0.0293250880411632 100-101 0.016870163007950065 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2276208.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.343320380989695 #Duplication Level Percentage of deduplicated Percentage of total 1 87.52599813841758 37.9366737897934 2 5.393454970798158 4.675404935194919 3 1.9281240575865766 2.5071389628680647 4 1.1403578561190297 1.9770758362698264 5 0.6952093759275205 1.5066341356347215 6 0.44935764824409075 1.168599150809502 7 0.31425792308573164 0.9534687289798498 8 0.30252318721290794 1.0489887540837752 9 0.20278158960352538 0.7910304664996772 >10 1.6563869487495775 14.371913729926547 >50 0.21741210519791934 6.51289826914697 >100 0.15907275902104426 13.46518334142932 >500 0.01007474397583889 2.9984137341422383 >1k 0.004278029087150635 3.3867762233390444 >5k 2.0304770668513606E-4 0.6696939694340809 >10k+ 5.076192667078016E-4 6.030105972447938 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT 59248 2.602925567434962 TruSeq Adapter, Index 27 (97% over 39bp) TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 31008 1.3622656628919678 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 24888 1.0933974399527635 No Hit GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 11042 0.4851050519108975 No Hit TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT 10850 0.4766699704069224 No Hit TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 9509 0.4177561980275968 No Hit GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 5710 0.25085580931092416 No Hit TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 4971 0.21838953206385356 No Hit TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 4649 0.20424319745822878 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3087 0.13562029480609855 No Hit GAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGC 2910 0.1278442040446216 No Hit TTGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 2666 0.11712462129998663 No Hit GGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCC 2658 0.11677315957065436 No Hit AGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCC 2656 0.11668529413832128 No Hit TGTGTTTGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAA 2482 0.1090410015253439 No Hit GTGTTTGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAAC 2324 0.1020996323710311 No Hit CTTTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAA 2306 0.10130884348003345 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0026359629699921976 0.0 0.0 2 0.0028556265508248805 0.0 0.0 3 0.003163155563990637 0.0 0.0 4 0.00338281914482332 0.0 0.0 5 0.0037342808741556133 0.0 0.0 6 0.004085742603487906 0.0 0.0 7 0.004744733345985955 0.0 0.0 8 0.005535522236983615 0.0 0.0 9 0.006282378411814738 0.0 0.0 10-11 0.009357668543472301 0.0 0.0 12-13 0.012718521330212354 0.0 0.0 14-15 0.016760331217533724 0.0 0.0 16-17 0.02201029079943485 0.0 0.0 18-19 0.028710030014831685 0.0 0.0 20-21 0.033191167063818416 0.0 0.0 22-23 0.037474606890055745 0.0 0.0 24-25 0.04230720566837477 0.0 0.0 26-27 0.04724963623711014 0.0 0.0 28-29 0.05287302390642683 0.0 0.0 30-31 0.10394480645002566 0.0 0.0 32-33 0.11180876264383571 0.0 0.0 34-35 0.11824490556223333 0.0 0.0 36-37 0.126240659904543 0.0 0.0 38-39 0.1342144478887694 0.0 0.0 40-41 0.14379178001307436 0.0 0.0 42-43 0.1543136655349599 0.0 0.0 44-45 0.16701022050708897 0.0 0.0 46-47 0.18003627085046708 0.0 0.0 48-49 0.19615957768358605 0.0 0.0 50-51 0.2147650829801143 0.0 0.0 52-53 0.23490823334247135 0.0 0.0 54-55 0.2590053281598167 0.0 0.0 56-57 0.28716619922256664 0.0 0.0 58-59 0.3172161770804777 0.0 0.0 60-61 0.3500119496987973 0.0 0.0 62-63 0.3872449266499371 0.0 0.0 64-65 0.4298816276895609 0.0 0.0 66-67 0.47811975004041807 0.0 0.0 68-69 0.5304436149947632 0.0 0.0 70-71 0.5870728861334289 0.0 0.0 72-73 0.6494573430899109 0.0 0.0 74-75 0.7193982272270373 0.0 0.0 76-77 0.7977522265100553 0.0 0.0 78-79 0.8835088884671348 0.0 0.0 80-81 0.9842246402789201 0.0 0.0 82-83 1.0968241918137533 0.0 0.0 84-85 1.2234602461637953 0.0 0.0 86-87 1.3641987024032953 0.0 0.0 88-89 1.515876404968263 0.0 2.1966358083268315E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACGTAC 20 0.0020756465 71.238686 8 CGGAAGA 11250 0.0 57.393578 4 TCGGAAG 11395 0.0 56.788334 3 GAAGAGC 11445 0.0 56.351906 6 GATCGGA 11780 0.0 55.202858 1 ATCGGAA 11880 0.0 54.46996 2 GGAAGAG 13380 0.0 48.41533 5 TTGTGTT 23435 0.0 44.080944 1 GCCGTCT 7445 0.0 43.57648 50-51 ATGCCGT 7455 0.0 43.421024 48-49 TGTGTTT 44325 0.0 43.185852 1 TCGTATG 7560 0.0 43.03597 44-45 TGCCGTC 7485 0.0 42.77246 50-51 TATGCCG 7470 0.0 42.666183 48-49 TCTCGTA 7410 0.0 42.496983 42-43 CGTATGC 7565 0.0 42.317356 46-47 ACTCGAT 7575 0.0 42.28866 36-37 GTATGCC 7655 0.0 42.285183 46-47 CTCGTAT 7375 0.0 42.21565 44-45 TCGATCT 7435 0.0 42.190674 38-39 >>END_MODULE