FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n01_30230-1.34100000035714.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n01_30230-1.34100000035714.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2276208
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT592482.602925567434962TruSeq Adapter, Index 27 (97% over 39bp)
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA310081.3622656628919678No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC248881.0933974399527635No Hit
GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC110420.4851050519108975No Hit
TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT108500.4766699704069224No Hit
TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT95090.4177561980275968No Hit
GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC57100.25085580931092416No Hit
TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT49710.21838953206385356No Hit
TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT46490.20424319745822878No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30870.13562029480609855No Hit
GAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGC29100.1278442040446216No Hit
TTGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT26660.11712462129998663No Hit
GGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCC26580.11677315957065436No Hit
AGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCC26560.11668529413832128No Hit
TGTGTTTGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAA24820.1090410015253439No Hit
GTGTTTGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAAC23240.1020996323710311No Hit
CTTTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAA23060.10130884348003345No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTAC200.002075646571.2386868
CGGAAGA112500.057.3935784
TCGGAAG113950.056.7883343
GAAGAGC114450.056.3519066
GATCGGA117800.055.2028581
ATCGGAA118800.054.469962
GGAAGAG133800.048.415335
TTGTGTT234350.044.0809441
GCCGTCT74450.043.5764850-51
ATGCCGT74550.043.42102448-49
TGTGTTT443250.043.1858521
TCGTATG75600.043.0359744-45
TGCCGTC74850.042.7724650-51
TATGCCG74700.042.66618348-49
TCTCGTA74100.042.49698342-43
CGTATGC75650.042.31735646-47
ACTCGAT75750.042.2886636-37
GTATGCC76550.042.28518346-47
CTCGTAT73750.042.2156544-45
TCGATCT74350.042.19067438-39
Produced by FastQC (version 0.11.2)