##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l07n02_53115004-1.3420000003588a.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4778339 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.330988864540586 33.0 33.0 33.0 22.0 33.0 2 29.622235885733517 33.0 33.0 33.0 15.0 33.0 3 29.307837932804684 33.0 33.0 33.0 15.0 33.0 4 32.50712998805652 37.0 37.0 37.0 15.0 37.0 5 32.64683753078214 37.0 37.0 37.0 15.0 37.0 6 33.56484962661712 37.0 37.0 37.0 22.0 37.0 7 32.506639859583004 37.0 37.0 37.0 15.0 37.0 8 33.13419767831458 37.0 37.0 37.0 15.0 37.0 9 32.19616586433068 37.0 33.0 37.0 6.0 37.0 10-11 32.32068967898678 37.0 33.0 37.0 15.0 37.0 12-13 32.30312792792642 37.0 33.0 37.0 10.5 37.0 14-15 33.69053503738433 37.0 33.0 40.0 15.0 40.0 16-17 33.56446884576418 37.0 33.0 40.0 10.5 40.0 18-19 33.62288349570845 37.0 33.0 40.0 10.5 40.0 20-21 33.65593012551014 37.0 33.0 40.0 10.5 40.0 22-23 34.85940386397868 37.0 33.0 40.0 22.0 40.0 24-25 33.91765977675506 37.0 33.0 40.0 15.0 40.0 26-27 33.74483371732311 37.0 33.0 40.0 10.5 40.0 28-29 33.30391397512818 37.0 33.0 40.0 15.0 40.0 30-31 33.30097194862064 37.0 33.0 40.0 10.5 40.0 32-33 33.02241720396983 37.0 33.0 40.0 6.0 40.0 34-35 33.25187968036592 37.0 33.0 40.0 10.5 40.0 36-37 33.04096643205934 37.0 33.0 40.0 6.0 40.0 38-39 33.369231651416946 37.0 33.0 40.0 10.5 40.0 40-41 32.77574665589863 37.0 33.0 40.0 6.0 40.0 42-43 32.62946423432913 37.0 33.0 40.0 6.0 40.0 44-45 32.512260536558834 37.0 33.0 40.0 6.0 40.0 46-47 32.47946085449358 37.0 33.0 40.0 6.0 40.0 48-49 32.40735441750784 37.0 33.0 40.0 6.0 40.0 50-51 31.56230240257127 37.0 30.0 38.5 10.5 38.5 52-53 32.00043759976008 37.0 33.0 38.5 10.5 40.0 54-55 32.5667263247752 37.0 33.0 40.0 10.5 40.0 56-57 32.94217195556866 37.0 33.0 40.0 10.5 40.0 58-59 32.21376235549633 37.0 33.0 40.0 6.0 40.0 60-61 32.12351268924202 37.0 33.0 40.0 6.0 40.0 62-63 31.67077838135804 37.0 33.0 40.0 6.0 40.0 64-65 31.499952493952396 37.0 33.0 37.0 6.0 40.0 66-67 31.447797759849188 37.0 33.0 37.0 6.0 40.0 68-69 31.737088661980657 37.0 33.0 37.0 15.0 40.0 70-71 31.244352169237054 37.0 33.0 37.0 10.5 40.0 72-73 31.869059520473535 37.0 30.0 37.0 15.0 37.0 74-75 32.07786031924482 37.0 27.0 37.0 22.0 37.0 76-77 31.70306721645325 37.0 27.0 37.0 22.0 37.0 78-79 31.033488938310988 33.0 27.0 37.0 15.0 37.0 80-81 30.322071958477622 33.0 27.0 37.0 15.0 37.0 82-83 29.95433978208746 33.0 27.0 37.0 6.0 37.0 84-85 29.68294861457088 33.0 27.0 37.0 6.0 37.0 86-87 29.50306727923657 33.0 27.0 37.0 6.0 37.0 88-89 29.334681570311357 33.0 27.0 37.0 6.0 37.0 90-91 29.203112629723424 33.0 27.0 37.0 6.0 37.0 92-93 29.013357047291954 33.0 27.0 37.0 6.0 37.0 94-95 28.889429464924945 33.0 27.0 37.0 6.0 37.0 96-97 28.752792131324295 33.0 27.0 37.0 6.0 37.0 98-99 28.60736596963924 33.0 27.0 37.0 6.0 37.0 100-101 27.46995859858415 33.0 21.0 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 -0.1977846508291492 2306 2 -0.35162610189595256 2306 3 -0.2039517637989725 2306 4 -0.1437421594093422 2306 5 -0.29071995739149514 2306 6 -0.47577064599347807 2306 7 0.15601509143397152 2306 8 -0.513439030626003 2306 9 -0.11474209306380345 2306 10-11 -0.18382991754916844 2306 12-13 -0.18383820747231994 2306 14-15 0.17790864677773754 2306 16-17 0.18446897856662758 2306 18-19 0.20182293715259192 2306 20-21 0.10792213070455858 2306 22-23 0.038772009063663404 2306 24-25 0.16842838416807382 2306 26-27 -0.18527803140734989 2306 28-29 0.056818491955532124 2306 30-31 0.21223214485923592 2306 32-33 0.16850197492025387 2306 34-35 -0.11048506020809867 2306 36-37 0.03171955101302615 2306 38-39 -0.16391626460917763 2306 40-41 0.10921867404420738 2306 42-43 0.06858419692932927 2306 44-45 0.14393894819769315 2306 46-47 0.15706882856883198 2306 48-49 0.04195710962769539 2306 50-51 0.18297772311228044 2306 52-53 0.11006680126299528 2306 54-55 0.06065932940447283 2306 56-57 0.05896002555169133 2306 58-59 0.21672226956730611 2306 60-61 0.1834461904223943 2306 62-63 0.2605047654202082 2306 64-65 0.24139364665579066 2306 66-67 0.1226826594834236 2306 68-69 0.06393350262511532 2306 70-71 0.19664135481312073 2306 72-73 -0.38338655754904494 2306 74-75 -0.2280161036699866 2306 76-77 0.07134709256014204 2306 78-79 0.09078568451774416 2306 80-81 0.3197397434297322 2306 82-83 0.3951392792438462 2306 84-85 0.31611407119385504 2306 86-87 0.3238444230133979 2306 88-89 0.3133922304085637 2306 90-91 0.22059371972800434 2306 92-93 0.3368684997975251 2306 94-95 0.2910191712892889 2306 96-97 0.2548825570258195 2306 98-99 0.26605550562259594 2306 100-101 0.20068809594252812 2307 1 -0.3073602153037207 2307 2 -0.529039589046036 2307 3 -0.5577406927669841 2307 4 -0.3624775077469451 2307 5 -0.42631623922596873 2307 6 -0.5431912457771944 2307 7 0.14770265448601805 2307 8 -0.6209741583268098 2307 9 -0.255312954490833 2307 10-11 -0.36839917681918166 2307 12-13 -0.4199984649061115 2307 14-15 -0.04486470878862292 2307 16-17 -0.16049552177086923 2307 18-19 -0.10714070495129846 2307 20-21 -0.19310575372239924 2307 22-23 -0.24069924283243438 2307 24-25 -0.029572684541413707 2307 26-27 -0.5647640256882198 2307 28-29 -0.23896387052750612 2307 30-31 -0.11934409277530733 2307 32-33 -0.02019397269302914 2307 34-35 -0.2353486591281424 2307 36-37 -0.325991079108249 2307 38-39 -0.48503742977469244 2307 40-41 -0.10818731943830784 2307 42-43 -0.11976138089956834 2307 44-45 -0.19164191083426374 2307 46-47 -0.24300025651424306 2307 48-49 -0.2681069040750188 2307 50-51 -0.24457860640587725 2307 52-53 -0.34824740477112925 2307 54-55 -0.1314482782103994 2307 56-57 -0.16962455128289378 2307 58-59 0.12781144602593741 2307 60-61 -0.1909651574942579 2307 62-63 -0.016602488377429836 2307 64-65 -0.022688732883050733 2307 66-67 -0.07469233416600929 2307 68-69 -0.24335799015701198 2307 70-71 -0.10444404759501325 2307 72-73 -0.4016607187300316 2307 74-75 -0.21824879747111225 2307 76-77 -0.09100534750865918 2307 78-79 -0.014251357425020927 2307 80-81 0.1877854203274616 2307 82-83 0.2103967856125557 2307 84-85 0.057064832535967724 2307 86-87 0.11000644953335836 2307 88-89 0.07661447117847331 2307 90-91 0.017301087472933574 2307 92-93 0.11194234780489865 2307 94-95 0.03408866280856415 2307 96-97 0.05469321944425687 2307 98-99 0.08189449700759965 2307 100-101 -0.20864537589362087 2201 1 -0.2892464327748563 2201 2 -0.5137394871454966 2201 3 -0.3432476067599062 2201 4 -0.2748434714555188 2201 5 -0.3802351699294846 2201 6 -0.5059142047107272 2201 7 0.07454326734498551 2201 8 -0.5562107509210321 2201 9 -0.18004371458372503 2201 10-11 -0.23786095278630626 2201 12-13 -0.2210908314981168 2201 14-15 0.04599908198017033 2201 16-17 -0.1823671871717849 2201 18-19 -0.034092213614904665 2201 20-21 -0.11570477371935084 2201 22-23 -0.13433634712666986 2201 24-25 0.03038379314689621 2201 26-27 -0.39399131024478606 2201 28-29 -0.16460810476965548 2201 30-31 -0.07350082288032667 2201 32-33 0.01285319167035226 2201 34-35 -0.24804369669538318 2201 36-37 -0.2581895815685584 2201 38-39 -0.32494294174251337 2201 40-41 -0.00660504573784948 2201 42-43 -0.07654012728945503 2201 44-45 -0.04515705034324213 2201 46-47 -0.12861616940987375 2201 48-49 -0.14333676395489903 2201 50-51 -0.22622736780617458 2201 52-53 -0.18494101545613262 2201 54-55 -0.02567230627562367 2201 56-57 0.056434357735852814 2201 58-59 0.24755076191899406 2201 60-61 0.0390870304833939 2201 62-63 0.10653550067993223 2201 64-65 0.05226232533072661 2201 66-67 -0.052628489425629255 2201 68-69 -0.10683405348641273 2201 70-71 0.051733981714438215 2201 72-73 -0.29614589268194536 2201 74-75 -0.0597224293327443 2201 76-77 0.19556636362390734 2201 78-79 0.20489664864781076 2201 80-81 0.3785583915522359 2201 82-83 0.31146732495756524 2201 84-85 0.3051527811489798 2201 86-87 0.27996482755690266 2201 88-89 0.12649046014244902 2201 90-91 0.20324592831170563 2201 92-93 0.23858329671592315 2201 94-95 0.1351447393493288 2201 96-97 0.1341025980020234 2201 98-99 0.0840823287508563 2201 100-101 -0.02878246611130919 1101 1 -0.18413649716693925 1101 2 -0.36829060392576807 1101 3 -0.3065280714240757 1101 4 -0.19426119199624026 1101 5 -0.18482728690754868 1101 6 -0.23203359466318574 1101 7 0.14184625560629627 1101 8 -0.3106675715925604 1101 9 -0.1439286921381182 1101 10-11 -0.07310297959713097 1101 12-13 -0.10844424008553233 1101 14-15 0.18243696962406375 1101 16-17 0.10949112259278593 1101 18-19 0.17509287377118454 1101 20-21 0.15985574999267982 1101 22-23 0.12264038355224471 1101 24-25 0.21670766491840965 1101 26-27 -0.14270616720399687 1101 28-29 0.05681327177020279 1101 30-31 0.17541889678057743 1101 32-33 0.11526213997833423 1101 34-35 0.013485529685361541 1101 36-37 7.157427834201258E-4 1101 38-39 -0.07726377735792056 1101 40-41 0.20425185563022552 1101 42-43 0.005552881246352115 1101 44-45 0.13243081995151584 1101 46-47 0.05436794644102605 1101 48-49 0.07439028812336801 1101 50-51 0.11021301691057062 1101 52-53 0.03756469126408035 1101 54-55 0.19114446001977115 1101 56-57 0.30757252117195577 1101 58-59 0.443637753931597 1101 60-61 0.24719442547772985 1101 62-63 0.3214730220996387 1101 64-65 0.21533844883373732 1101 66-67 0.2802878222049543 1101 68-69 0.23726353401722022 1101 70-71 0.3265308831631124 1101 72-73 0.03475893193938262 1101 74-75 0.2775158728884577 1101 76-77 0.4203720245828926 1101 78-79 0.4385507978939067 1101 80-81 0.5033641473367716 1101 82-83 0.4174931568601501 1101 84-85 0.32784963247444665 1101 86-87 0.26443303440651533 1101 88-89 0.40219764737400254 1101 90-91 0.2804641923134703 1101 92-93 0.4356421320985291 1101 94-95 0.4990487768395582 1101 96-97 0.2919667536037984 1101 98-99 0.33224486638375694 1101 100-101 0.39869653259724913 2304 1 -0.038125241015052325 2304 2 -0.16619212369429803 2304 3 -0.05857726304994415 2304 4 -0.03135644828780215 2304 5 0.045701805386194394 2304 6 0.08973759169722229 2304 7 0.38314759071749904 2304 8 -0.03048068627759193 2304 9 0.05976048188437488 2304 10-11 0.06564759065359027 2304 12-13 0.009636499923544761 2304 14-15 0.2107779178455118 2304 16-17 0.16857503451765865 2304 18-19 0.25080311953846746 2304 20-21 0.15127958209729542 2304 22-23 0.11513841462335961 2304 24-25 0.3183641593159976 2304 26-27 0.09134193926674072 2304 28-29 0.21194175365711487 2304 30-31 0.30194973810235837 2304 32-33 0.2566007684615599 2304 34-35 0.12037037671539252 2304 36-37 0.20716700134990873 2304 38-39 0.11500504367248965 2304 40-41 0.3562627430894949 2304 42-43 0.23418178575850135 2304 44-45 0.26271018943623403 2304 46-47 0.2628522804680671 2304 48-49 0.2603444704308444 2304 50-51 0.16589355521311688 2304 52-53 0.0591187900502419 2304 54-55 0.34414822342711204 2304 56-57 0.4876048256012311 2304 58-59 0.5146637797007756 2304 60-61 0.3672037332700526 2304 62-63 0.4466807827966406 2304 64-65 0.42278816745821146 2304 66-67 0.37655218697427983 2304 68-69 0.3333275423365585 2304 70-71 0.38025691231103664 2304 72-73 0.3025105699567341 2304 74-75 0.3879388897130269 2304 76-77 0.4742693233298034 2304 78-79 0.5445385117211323 2304 80-81 0.5302714283516572 2304 82-83 0.41135208499661147 2304 84-85 0.38812592857816597 2304 86-87 0.560938562039162 2304 88-89 0.36069887510064547 2304 90-91 0.5276546428352127 2304 92-93 0.5238475659888167 2304 94-95 0.4930980241651568 2304 96-97 0.3901282667024937 2304 98-99 0.4541087701742086 2304 100-101 0.4945176674778189 2202 1 -0.10749567526842796 2202 2 -0.19867162080452516 2202 3 -0.17625700083090834 2202 4 -0.14904274000262774 2202 5 -0.13753436266489416 2202 6 -0.10919640453439428 2202 7 0.16725227066320514 2202 8 -0.1661269414240394 2202 9 -0.058557943177049765 2202 10-11 -0.04644671762752495 2202 12-13 -0.07034353242461577 2202 14-15 -0.01563588777200664 2202 16-17 0.006252919205948615 2202 18-19 0.07194356571486793 2202 20-21 0.09302346310047227 2202 22-23 0.09969869577941637 2202 24-25 -0.04477132488992197 2202 26-27 0.06799753364042971 2202 28-29 0.07579881313059644 2202 30-31 0.20459400625026802 2202 32-33 0.08476921638465029 2202 34-35 -0.060555593902940075 2202 36-37 -0.058683880462616855 2202 38-39 -0.09851672425502045 2202 40-41 0.08139377678879356 2202 42-43 0.01104346922507915 2202 44-45 0.05449151781839845 2202 46-47 0.015393664705158017 2202 48-49 0.024971244458662056 2202 50-51 0.027735794057484497 2202 52-53 -0.06925619674551342 2202 54-55 0.11866289177365275 2202 56-57 0.31056244632276275 2202 58-59 0.23169678556873663 2202 60-61 0.1187566943245244 2202 62-63 0.17915382877981045 2202 64-65 0.16851673744381657 2202 66-67 0.17472809867145855 2202 68-69 0.19157944625434098 2202 70-71 0.2190744104405944 2202 72-73 0.1341371175525623 2202 74-75 0.3283947942034473 2202 76-77 0.3465868046247458 2202 78-79 0.26191155109897224 2202 80-81 0.43784078503550106 2202 82-83 0.408435905698461 2202 84-85 0.27788021517218553 2202 86-87 0.1746828552976325 2202 88-89 0.21232526591609258 2202 90-91 0.12115081443827691 2202 92-93 0.17635632191595008 2202 94-95 0.2313515755620088 2202 96-97 0.1851658078926839 2202 98-99 0.1655237696357581 2202 100-101 0.10571800632904882 1102 1 -0.14232609021875575 1102 2 -0.37589861473528785 1102 3 -0.31998009909293046 1102 4 -0.4316954473925634 1102 5 -0.3845973917506669 1102 6 -0.12863140350678037 1102 7 -0.39803159350511663 1102 8 -0.1564883390919931 1102 9 -0.4485549772284756 1102 10-11 -0.27636008660418554 1102 12-13 -0.2838755145061995 1102 14-15 -0.47349969375124346 1102 16-17 -0.43445087750043854 1102 18-19 -0.36169905744991837 1102 20-21 -0.5322982741369344 1102 22-23 -0.21604759337573398 1102 24-25 -0.3255149768346399 1102 26-27 -0.2894928988620791 1102 28-29 -0.3790021274014066 1102 30-31 -0.2228550076814102 1102 32-33 -0.41013388135139195 1102 34-35 -0.286135037492933 1102 36-37 -0.43725288089411407 1102 38-39 -0.2826723006766585 1102 40-41 -0.3577107088553433 1102 42-43 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58-59 -0.6583785751954423 1213 60-61 -0.2405736616549774 1213 62-63 -0.4749910627295151 1213 64-65 -0.4229892771341994 1213 66-67 -0.34439808459248766 1213 68-69 -0.12497617164553887 1213 70-71 -0.24229740880308626 1213 72-73 0.21587658028962053 1213 74-75 -0.08555890474471184 1213 76-77 -0.19378787487099558 1213 78-79 -0.270055369464135 1213 80-81 -0.4741009569968746 1213 82-83 -0.3449988212779296 1213 84-85 -0.4671026500344375 1213 86-87 -0.43379374628353773 1213 88-89 -0.4158235595531181 1213 90-91 -0.4300038090531295 1213 92-93 -0.34145764525794675 1213 94-95 -0.31502631813003745 1213 96-97 -0.34158290918316325 1213 98-99 -0.3802304828904255 1213 100-101 -0.1897456358791807 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 709.0 3 434.0 4 556.0 5 427.0 6 975.0 7 7656.0 8 47950.0 9 144915.0 10 172817.0 11 120582.0 12 71064.0 13 45695.0 14 31337.0 15 23168.0 16 19711.0 17 17938.0 18 17366.0 19 17303.0 20 18070.0 21 19360.0 22 20646.0 23 21806.0 24 23821.0 25 27643.0 26 32218.0 27 39508.0 28 49047.0 29 59118.0 30 70608.0 31 90316.0 32 123487.0 33 177475.0 34 270201.0 35 456960.0 36 882605.0 37 1341743.0 38 312998.0 39 106.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.196308362026244 22.19619930205815 22.28145483480666 13.326037501108951 2 30.000570370988584 28.74959161856608 29.5345437220817 11.715294288363635 3 34.7629612749292 20.953303791500094 29.69428039108932 14.589454542481391 4 26.146699665228233 17.805116342148438 33.8746430564991 22.173540936124223 5 37.257175057010116 16.13689220130116 29.109791065626933 17.49614167606179 6 33.91413734479197 16.23199442253801 34.71848548520839 15.135382747461632 7 36.54575062470169 19.989301603837507 27.068471830699664 16.39647594076114 8 25.805394564872596 34.23812634640986 23.906079308962013 16.050399779755526 9 34.70485287684962 26.96041995920741 21.869788847232254 16.464938316710718 10-11 27.836974081434413 31.50925700461083 25.430933136437066 15.222835777517693 12-13 30.670538707593725 23.70159835352009 25.556811036762316 20.07105190212387 14-15 26.135940091711152 23.94823077756284 28.18497436323978 21.730854767486225 16-17 29.037151078847316 24.92559854259874 28.93807133992594 17.099179038628005 18-19 29.07234136507149 25.250454152394102 29.188508436173993 16.488696046360413 20-21 27.045709881696332 28.71994147767611 27.556177639218337 16.67817100140922 22-23 31.612760927717858 25.009169138825683 26.71512119286988 16.66294874058658 24-25 25.171078500848758 31.331291377525883 26.562778584028006 16.934851537597346 26-27 22.513993305019977 33.97039783026454 26.387489005073128 17.128119859642354 28-29 24.572122939690207 31.5287673562889 26.15168505198455 17.747424652036337 30-31 28.900856606334475 26.956023320556604 26.511178818537783 17.631941254571142 32-33 23.989253488201882 26.314693449480554 28.980025085819634 20.71602797649793 34-35 19.18783990121802 26.18297105514314 36.31549543764566 18.313693605993187 36-37 21.889884858184917 26.81101434546026 29.170916643273664 22.12818415308116 38-39 19.61237256879539 26.20975885671646 26.129392442914572 28.04847613157358 40-41 22.650466321102265 25.674970998783746 28.394369145033764 23.280193535080226 42-43 25.76423931071768 25.840924119060716 30.675632804359875 17.71920376586173 44-45 22.11522359907889 29.029738447331116 31.39467738531007 17.46036056827992 46-47 24.88578397781144 30.385552095322055 27.37385896720315 17.354804959663355 48-49 19.98479027962123 28.913747651401682 31.044503218170878 20.05695885080621 50-51 18.927800122863598 26.44411394644464 32.28391013297499 22.344175797716765 52-53 27.400982813298086 25.992447334719515 26.676996101610012 19.929573750372384 54-55 29.730707309534782 26.057980501924266 26.503810164044012 17.70750202449694 56-57 30.053655727639356 25.911866372975982 26.053371375559475 17.981106523825183 58-59 27.100606617487095 25.90554112609485 26.322065772688973 20.671786483729086 60-61 21.280392830408967 26.25359402527791 25.63956589824662 26.826447246066504 62-63 25.226013210193592 26.519120905197397 26.65588072464257 21.598985159966436 64-65 20.930261809343666 28.825860907667494 30.656046347479897 19.58783093550894 66-67 19.480993661618662 28.46251272624197 28.339946646226405 23.71654696591296 68-69 19.05216430683806 26.300831999567297 36.00410439003907 18.642899303555577 70-71 18.526310449489525 33.20878666148747 30.627737865870696 17.637165023152306 72-73 18.22882202553158 38.770299291886445 25.52159396772825 17.47928471485372 74-75 18.892382237819437 38.54848592006167 24.925078390736026 17.63405345138286 76-77 19.187735979288696 37.6142692413035 25.315679375600155 17.88231540380766 78-79 18.8818247414079 36.859494274309085 26.21738800491546 18.041292979367558 80-81 18.19555650128066 36.72803106497324 26.13946219105755 18.936950242688553 82-83 17.822387597900562 35.881826172193435 26.474470388863626 19.821315841042374 84-85 18.22541901055037 34.616813348449384 26.889910395169014 20.26785724583123 86-87 18.413393289745507 34.1339486250564 27.667996208180178 19.784661877017918 88-89 18.94326739021816 34.15901949877081 27.303909977764974 19.593803133246055 90-91 19.398582822502025 33.28257782089643 26.92486279604514 20.393976560556403 92-93 19.090220720198516 33.670673732541715 26.657592192458235 20.58151335480153 94-95 18.690944980931327 34.16851707124002 26.512349354147307 20.628188593681344 96-97 19.323445989952617 34.27362996900958 26.297573610753965 20.10535043028383 98-99 19.99849864820675 32.98427952570284 26.11960007427078 20.897621751819628 100-101 20.101340139672985 33.17613490460461 25.522819514037497 21.19970544168491 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 220.0 1 301.0 2 371.0 3 484.0 4 514.0 5 422.0 6 450.5 7 584.0 8 773.5 9 926.5 10 1052.5 11 1226.0 12 1303.5 13 1279.5 14 1252.5 15 1251.0 16 1236.0 17 1177.0 18 1247.0 19 1368.0 20 1564.0 21 1935.5 22 2419.5 23 3160.0 24 4186.5 25 5448.5 26 7368.5 27 10032.0 28 12923.5 29 16153.5 30 20116.5 31 25209.0 32 33173.5 33 48443.5 34 73640.0 35 115536.0 36 168725.5 37 229803.0 38 298158.5 39 334651.0 40 332311.0 41 300415.0 42 268218.5 43 272840.0 44 270474.0 45 254769.5 46 256007.5 47 238201.0 48 192571.0 49 155037.0 50 131726.5 51 117019.0 52 104006.5 53 90054.0 54 76934.5 55 63018.0 56 50198.0 57 40154.0 58 31900.0 59 25025.0 60 19407.0 61 14928.0 62 11201.5 63 8271.5 64 6196.5 65 4538.0 66 3314.5 67 2471.0 68 1842.0 69 1307.5 70 952.0 71 725.5 72 536.0 73 381.0 74 285.5 75 222.5 76 168.0 77 137.5 78 116.0 79 82.5 80 69.5 81 57.5 82 50.0 83 43.5 84 27.0 85 19.0 86 20.5 87 18.5 88 15.0 89 15.5 90 13.0 91 11.5 92 11.0 93 7.0 94 5.5 95 4.5 96 3.0 97 3.0 98 2.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21597463051491322 2 0.19908591667522962 3 0.1753747484219935 4 0.15959520661886903 5 0.17020558817614237 6 0.1313427113480228 7 0.11847213016908176 8 0.11428657531414159 9 0.10302743275435251 10-11 0.08897443232889087 12-13 0.1148934807681079 14-15 0.11112648139866176 16-17 0.10277629946305611 18-19 0.09649796718064582 20-21 0.09995104993597147 22-23 0.08731067427405213 24-25 0.404732690585578 26-27 0.19031717925412994 28-29 0.09252169006845266 30-31 0.1772582481067166 32-33 0.20735238751373647 34-35 0.20630599880000142 36-37 0.21295056713221897 38-39 0.2130028865679057 40-41 0.2093719177312451 42-43 0.2254235205999407 44-45 0.20822089014613654 46-47 0.22681521758920828 48-49 0.2162990110161711 50-51 0.21869524117062436 52-53 0.21112985077031998 54-55 0.19364469536380738 56-57 0.2009694163599527 58-59 0.1957479366784148 60-61 0.176703662088437 62-63 0.1659991055469275 64-65 0.16976610491637364 66-67 0.18138101963883266 68-69 0.1742132569497476 70-71 0.16683621651791553 72-73 0.17013234096618093 74-75 0.16085087307535106 76-77 0.17140893519693767 78-79 0.16920105501095672 80-81 0.17446439024104402 82-83 0.199514936047861 84-85 0.1978825696544343 86-87 0.2020053411865504 88-89 0.20847202343743299 90-91 0.18982537655867449 92-93 0.17123104911560272 94-95 0.17183795456956905 96-97 0.18078457807200368 98-99 0.19470154796467978 100-101 0.20600254607301827 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4778339.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.28087928386382 #Duplication Level Percentage of deduplicated Percentage of total 1 91.39579807916614 43.212736960334475 2 3.9240291948283517 3.710631013340733 3 1.1950992394783149 1.6951602862203496 4 0.7886173364163288 1.4914608433705063 5 0.393547527146451 0.9303636561737235 6 0.3206403366739817 0.9096094231091988 7 0.24562614153755818 0.8129393962898976 8 0.18931560648312976 0.716080666934426 9 0.1429219573072748 0.6081728229412918 >10 1.0724929260553884 9.968224657765878 >50 0.1577044304789886 5.328633937591649 >100 0.13937135953058868 13.456015905279461 >500 0.023822582439232198 7.8254464202158704 >1k 0.010747667857677981 8.113860591650512 >5k 2.2134560470858915E-4 0.8098133239839711 >10k+ 4.4268995945756845E-5 0.4108500947979859 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT 19629 0.4107912812381038 Illumina Single End PCR Primer 1 (96% over 32bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 9833 0.2057828044431339 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 8965 0.18761749637269354 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 7615 0.15936500110184731 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 6306 0.13197054457626384 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 5971 0.12495974019423904 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 4942 0.10342506046557183 No Hit TTGTGTTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTA 4838 0.10124857194100292 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.005964415668289755 0.0 0.0 2 0.0066131766708054825 0.0 0.0 3 0.0071572988019477065 0.0 0.0 4 0.007450287641793518 0.0 0.0 5 0.008140904192858648 0.0 0.0 6 0.00889430406674788 0.0 0.0 7 0.010024403877581729 0.0 2.0927774274700895E-5 8 0.011070792591316773 0.0 2.0927774274700895E-5 9 0.012012542433678313 0.0 2.0927774274700895E-5 10-11 0.017527010955061997 0.0 3.139166141205134E-5 12-13 0.022329935151105856 0.0 4.185554854940179E-5 14-15 0.027404920412720822 0.0 4.185554854940179E-5 16-17 0.031056817023656126 0.0 5.231943568675223E-5 18-19 0.035054021910123995 0.0 6.278332282410268E-5 20-21 0.039804626670481104 0.0 6.278332282410268E-5 22-23 0.045005178577744276 0.0 6.278332282410268E-5 24-25 0.05037315267920506 0.0 7.324720996145313E-5 26-27 0.05640035167031891 0.0 8.371109709880358E-5 28-29 0.06345301160089312 0.0 8.371109709880358E-5 30-31 0.09961620554757626 0.0 8.371109709880358E-5 32-33 0.10812334579024219 0.0 8.371109709880358E-5 34-35 0.11514461405940432 0.0 9.417498423615403E-5 36-37 0.12335876546222443 0.0 1.0463887137350448E-4 38-39 0.13182405015634094 0.0 1.2556664564820536E-4 40-41 0.14127294024136838 0.0 1.4649441992290626E-4 42-43 0.1514961579745598 0.0 1.4649441992290626E-4 44-45 0.1625564866787392 0.0 1.4649441992290626E-4 46-47 0.17399351531986323 0.0 1.4649441992290626E-4 48-49 0.18633043825479942 0.0 1.4649441992290626E-4 50-51 0.19960911103209714 0.0 1.4649441992290626E-4 52-53 0.2142480891372504 0.0 1.4649441992290626E-4 54-55 0.2304880419744183 0.0 1.4649441992290626E-4 56-57 0.24595366716342226 0.0 1.4649441992290626E-4 58-59 0.26345975034420954 0.0 1.569583070602567E-4 60-61 0.2826819110155223 0.0 1.778860813349576E-4 62-63 0.30363061306449796 0.0 1.988138556096585E-4 64-65 0.3254373538587363 0.0 2.0927774274700895E-4 66-67 0.3494205831775435 0.0 2.0927774274700895E-4 68-69 0.3755279815852328 0.0 2.0927774274700895E-4 70-71 0.40274455202948134 0.0 2.197416298843594E-4 72-73 0.4322108582082602 0.0 2.3020551702170985E-4 74-75 0.46498375272244186 0.0 2.3020551702170985E-4 76-77 0.5025595714326673 0.0 2.3020551702170985E-4 78-79 0.5434105868168835 0.0 2.4066940415906027E-4 80-81 0.5874321600037167 0.0 2.8252495270846207E-4 82-83 0.6383389709269267 0.0 3.139166141205134E-4 84-85 0.6950323114370913 0.0 3.139166141205134E-4 86-87 0.7575958926313098 0.0 3.139166141205134E-4 88-89 0.8270342476747673 0.0 3.348443883952143E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGGA 10990 0.0 90.548195 2 GAGCGGC 6485 0.0 89.98537 9 CGGGAGA 9425 0.0 88.64677 4 AGAGCGG 6970 0.0 88.01212 8 GAGAGCG 8265 0.0 83.75173 7 TCGGGAG 9445 0.0 83.68603 3 AAGCGGC 2715 0.0 83.00429 9 CGGAAAG 3765 0.0 81.07325 5 ATTCGGA 1160 0.0 80.62959 2 ATCCGGA 1585 0.0 79.97757 2 GATCGGG 12860 0.0 79.09705 1 GGATCGG 2360 0.0 78.27434 1 TCGGGAA 3615 0.0 76.55983 3 TCGGAGA 1745 0.0 76.1718 4 GAACCGG 1285 0.0 76.128265 1 AAAGCGG 3215 0.0 75.70301 8 AACCGGA 1290 0.0 75.448456 2 GTTCGGA 795 0.0 75.247086 2 CGGAGAG 3415 0.0 74.647484 5 GATCCGG 1775 0.0 73.30508 1 >>END_MODULE