FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n02_53115004-1.3420000003588a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n02_53115004-1.3420000003588a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4778339
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT196290.4107912812381038Illumina Single End PCR Primer 1 (96% over 32bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC98330.2057828044431339No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG89650.18761749637269354No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA76150.15936500110184731No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC63060.13197054457626384No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG59710.12495974019423904No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA49420.10342506046557183No Hit
TTGTGTTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTA48380.10124857194100292No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA109900.090.5481952
GAGCGGC64850.089.985379
CGGGAGA94250.088.646774
AGAGCGG69700.088.012128
GAGAGCG82650.083.751737
TCGGGAG94450.083.686033
AAGCGGC27150.083.004299
CGGAAAG37650.081.073255
ATTCGGA11600.080.629592
ATCCGGA15850.079.977572
GATCGGG128600.079.097051
GGATCGG23600.078.274341
TCGGGAA36150.076.559833
TCGGAGA17450.076.17184
GAACCGG12850.076.1282651
AAAGCGG32150.075.703018
AACCGGA12900.075.4484562
GTTCGGA7950.075.2470862
CGGAGAG34150.074.6474845
GATCCGG17750.073.305081
Produced by FastQC (version 0.11.2)