FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n02_52370180-2.342000000358cd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n02_52370180-2.342000000358cd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5323333
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT103880.19514090138640583Illumina Single End PCR Primer 1 (97% over 34bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC94280.1771070868570499No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC85070.15980589604294904No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG82450.1548841674943123No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG69740.13100814846638376No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA66130.1242266827944072No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA54340.1020789043255419No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC54150.088.0683069
CGGGAGA81450.087.135314
TCGGGAG83400.084.8702853
AGAGCGG58500.083.953068
GAGAGCG73550.082.904987
GTTCGGA11300.082.780082
AAGCGGC16750.081.871939
TCGGAGA20250.079.94174
CGGAGAG31150.079.095795
ATTCGGA16050.078.102872
CGGAAAG30600.077.5697865
ATCCGGA13500.077.027962
ATCGGGA106000.076.77912
GATCGGG110450.075.379351
TTCGGAG15000.075.006883
GATCCGG14850.073.879121
AGAGCGT117750.073.7869348
GAGCGTC117650.073.1639949
AAAGCGG20500.070.598918
CCGGAGA13600.069.1146854
Produced by FastQC (version 0.11.2)