##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l07n02_52370180-2.342000000358cd.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5323333 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.948158794499612 33.0 33.0 33.0 27.0 33.0 2 30.43485143612094 33.0 33.0 33.0 22.0 33.0 3 30.264601519386446 33.0 33.0 33.0 22.0 33.0 4 33.60223904835561 37.0 37.0 37.0 15.0 37.0 5 33.646640366101465 37.0 37.0 37.0 15.0 37.0 6 34.31675136610841 37.0 37.0 37.0 27.0 37.0 7 33.699349073221605 37.0 37.0 37.0 22.0 37.0 8 33.993251220616855 37.0 37.0 37.0 27.0 37.0 9 33.411067088983536 37.0 37.0 37.0 15.0 37.0 10-11 33.468309328009354 37.0 37.0 37.0 15.0 37.0 12-13 33.466930962237385 37.0 37.0 37.0 15.0 37.0 14-15 35.07013049906891 40.0 37.0 40.0 15.0 40.0 16-17 34.99309389437031 40.0 37.0 40.0 15.0 40.0 18-19 35.06386647237736 40.0 37.0 40.0 15.0 40.0 20-21 35.02183998258234 40.0 37.0 40.0 15.0 40.0 22-23 35.92420876169122 40.0 37.0 40.0 24.5 40.0 24-25 35.20989613086388 40.0 37.0 40.0 22.0 40.0 26-27 35.01777345133209 40.0 37.0 40.0 18.5 40.0 28-29 34.73162800448516 40.0 37.0 40.0 15.0 40.0 30-31 34.717982230305715 40.0 37.0 40.0 15.0 40.0 32-33 34.51977567437543 40.0 37.0 40.0 15.0 40.0 34-35 34.814818084835196 40.0 37.0 40.0 18.5 40.0 36-37 34.44273822434178 40.0 37.0 40.0 15.0 40.0 38-39 34.39555659959653 40.0 37.0 40.0 15.0 40.0 40-41 34.942311987621295 37.0 37.0 40.0 18.5 40.0 42-43 34.64083498064089 37.0 37.0 40.0 15.0 40.0 44-45 34.16585990393612 37.0 37.0 40.0 15.0 40.0 46-47 34.022660990774014 37.0 37.0 40.0 15.0 40.0 48-49 33.93214692749824 37.0 35.0 40.0 15.0 40.0 50-51 33.02544270666517 37.0 33.0 38.5 15.0 38.5 52-53 33.43732047196747 37.0 33.0 38.5 15.0 40.0 54-55 34.13590348377605 37.0 37.0 40.0 15.0 40.0 56-57 34.49497138352983 37.0 37.0 40.0 18.5 40.0 58-59 33.95930707321898 37.0 33.0 40.0 15.0 40.0 60-61 33.75641510685129 37.0 33.0 40.0 15.0 40.0 62-63 33.44432510609425 37.0 33.0 40.0 10.5 40.0 64-65 33.24625587014752 37.0 33.0 40.0 10.5 40.0 66-67 33.12875363987186 37.0 33.0 40.0 15.0 40.0 68-69 33.23783013386538 37.0 33.0 37.0 15.0 40.0 70-71 32.84746210691685 37.0 33.0 37.0 15.0 40.0 72-73 33.14492029711461 37.0 33.0 37.0 18.5 40.0 74-75 33.244944943327795 37.0 33.0 37.0 22.0 40.0 76-77 32.979576517193266 37.0 33.0 37.0 22.0 37.0 78-79 32.525244898262045 37.0 33.0 37.0 22.0 37.0 80-81 31.987466968532683 37.0 33.0 37.0 15.0 37.0 82-83 31.62737010816344 37.0 33.0 37.0 15.0 37.0 84-85 31.352364768463666 37.0 33.0 37.0 15.0 37.0 86-87 31.16268228946038 37.0 33.0 37.0 15.0 37.0 88-89 30.99057658049947 35.0 33.0 37.0 15.0 37.0 90-91 30.848843196546223 33.0 33.0 37.0 15.0 37.0 92-93 30.666815977884532 33.0 33.0 37.0 15.0 37.0 94-95 30.556938481962334 33.0 33.0 37.0 15.0 37.0 96-97 30.442276013918345 33.0 30.0 37.0 15.0 37.0 98-99 30.306464108106706 33.0 27.0 37.0 15.0 37.0 100-101 29.166426184497567 33.0 27.0 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 -0.10664368223805454 2306 2 -0.16168992149886918 2306 3 -0.13886442893253914 2306 4 0.05717712434132238 2306 5 -0.017921637314735506 2306 6 -0.20759166201425217 2306 7 0.2158533945540526 2306 8 -0.3336528981314473 2306 9 0.012358070588902592 2306 10-11 -0.050658120569188725 2306 12-13 -0.02111829704085011 2306 14-15 0.4035798870338212 2306 16-17 0.2712667996535174 2306 18-19 0.28922524725557963 2306 20-21 0.2645273929663148 2306 22-23 0.1926019358592299 2306 24-25 0.384383788056212 2306 26-27 -0.006660934452433764 2306 28-29 0.14396188067140514 2306 30-31 0.24557627918422043 2306 32-33 0.3449716776397196 2306 34-35 0.03344793039776306 2306 36-37 0.37462275664362465 2306 38-39 0.2018855783578246 2306 40-41 -0.12058051995362717 2306 42-43 0.3426429801897939 2306 44-45 0.5767719301952567 2306 46-47 0.4898338833220066 2306 48-49 0.23034697346902533 2306 50-51 0.12960458817269682 2306 52-53 0.12084174300294848 2306 54-55 0.14933290217603457 2306 56-57 0.22361005891710306 2306 58-59 0.47844144219143914 2306 60-61 0.4339219004609447 2306 62-63 0.40554610764633736 2306 64-65 0.32979885269737963 2306 66-67 0.2875087130904532 2306 68-69 0.037607616553273715 2306 70-71 0.23858529816926932 2306 72-73 -0.24513553092302232 2306 74-75 -0.2021802274988076 2306 76-77 -0.04010481415101452 2306 78-79 -0.02659377742091351 2306 80-81 0.3657881611223317 2306 82-83 0.3293075502629925 2306 84-85 0.3377449097339955 2306 86-87 0.32658876681418647 2306 88-89 0.3680549849351955 2306 90-91 0.21081704744068475 2306 92-93 0.32300716773365323 2306 94-95 0.3618886514030599 2306 96-97 0.18296480430562667 2306 98-99 0.20172455604631168 2306 100-101 0.23084820502604586 2307 1 -0.06870410327787013 2307 2 -0.13455977552227694 2307 3 -0.06565653834313068 2307 4 0.0951810804822486 2307 5 0.036887255019202314 2307 6 -0.16508521925280206 2307 7 0.36291959292666576 2307 8 -0.23408109835076374 2307 9 0.03744250556314199 2307 10-11 -0.03788055553606995 2307 12-13 -0.058053591981490626 2307 14-15 0.30080993571483106 2307 16-17 0.28899618622858725 2307 18-19 0.24295555777668199 2307 20-21 0.2414671011555285 2307 22-23 0.031141622878152475 2307 24-25 0.2860360447167025 2307 26-27 -0.1670967009188402 2307 28-29 0.16255220078797805 2307 30-31 0.2565011460895903 2307 32-33 0.2787997085678171 2307 34-35 0.014719154842246951 2307 36-37 0.23123795400151437 2307 38-39 -0.024683797874160973 2307 40-41 -0.1726420790158656 2307 42-43 0.17639549698155577 2307 44-45 0.3429271315332585 2307 46-47 0.2831739364067758 2307 48-49 0.04567180410956695 2307 50-51 -0.10615170841991528 2307 52-53 -0.017457470029036415 2307 54-55 0.09087667171688452 2307 56-57 0.06943492877729796 2307 58-59 0.22351314650402543 2307 60-61 0.27555320516745496 2307 62-63 0.24379686869141892 2307 64-65 0.29081784675438627 2307 66-67 0.12907113124688152 2307 68-69 -0.0077966596816736455 2307 70-71 -0.02830869806825831 2307 72-73 -0.2146084948211282 2307 74-75 -0.2129147812177905 2307 76-77 -0.036251083377699445 2307 78-79 0.0704714360105072 2307 80-81 0.2836715960608558 2307 82-83 0.4074498479219564 2307 84-85 0.31626660555591357 2307 86-87 0.19991779330423753 2307 88-89 0.2890266258674039 2307 90-91 0.2918318430375031 2307 92-93 0.3355970006009983 2307 94-95 0.15834542037584498 2307 96-97 0.15928895391273556 2307 98-99 0.22836436744075073 2307 100-101 0.15019745200289591 2201 1 -0.014311691171400298 2201 2 -0.09815900794294663 2201 3 -0.019502119415800934 2201 4 0.09157598656319976 2201 5 -0.021101851492758783 2201 6 -0.1340254072248399 2201 7 0.3509990940954495 2201 8 -0.1980226677022614 2201 9 0.06433638588188018 2201 10-11 0.08274111289336616 2201 12-13 0.03791749053646498 2201 14-15 0.39916653683578573 2201 16-17 0.21679589733071936 2201 18-19 0.25645488138882655 2201 20-21 0.2845958223386731 2201 22-23 0.174676199475698 2201 24-25 0.33048632720142734 2201 26-27 7.756932172142683E-4 2201 28-29 0.20207384168062248 2201 30-31 0.25205655484283795 2201 32-33 0.29853481969225015 2201 34-35 0.07085253639332478 2201 36-37 0.37345392047792103 2201 38-39 0.15640279044093575 2201 40-41 -0.041166820237066304 2201 42-43 0.28655086074066105 2201 44-45 0.5101199028664851 2201 46-47 0.3756035456455109 2201 48-49 0.25123934221441857 2201 50-51 0.16569156709979183 2201 52-53 0.2270188849997865 2201 54-55 0.33711505172767176 2201 56-57 0.4127446140747679 2201 58-59 0.5896038449054117 2201 60-61 0.5385158679676181 2201 62-63 0.4450445029230181 2201 64-65 0.527023461851897 2201 66-67 0.31082934012552954 2201 68-69 0.22116431822148286 2201 70-71 0.3410114997879603 2201 72-73 0.03145009019682021 2201 74-75 0.09712045814677595 2201 76-77 0.18972721591150332 2201 78-79 0.3435225388779912 2201 80-81 0.548232209561327 2201 82-83 0.5260203891934232 2201 84-85 0.5145062491259615 2201 86-87 0.4160173565976386 2201 88-89 0.4232550522276206 2201 90-91 0.4018337454138141 2201 92-93 0.4992416584990238 2201 94-95 0.41497785788970987 2201 96-97 0.41612297975191126 2201 98-99 0.407631609769215 2201 100-101 0.3221104094330265 1101 1 -0.0265444301553579 1101 2 -0.09421503694518663 1101 3 -0.06305128872789822 1101 4 0.08548654533338151 1101 5 0.12489042345873003 1101 6 -0.0830362000299445 1101 7 0.3768850709897791 1101 8 -0.06513701606532152 1101 9 0.12414710111664817 1101 10-11 0.13924065805197472 1101 12-13 0.08268356840357427 1101 14-15 0.37417038410863057 1101 16-17 0.19784070437026458 1101 18-19 0.3074027032604647 1101 20-21 0.23507080299109617 1101 22-23 0.10958500295742368 1101 24-25 0.2728587605832331 1101 26-27 0.04284815052466229 1101 28-29 0.17379214971091983 1101 30-31 0.3212992314940877 1101 32-33 0.21823484785439007 1101 34-35 -0.008174419723829374 1101 36-37 0.24938183344069387 1101 38-39 0.10626324008412524 1101 40-41 -0.009372644939318775 1101 42-43 0.26239252912574784 1101 44-45 0.34156682802964866 1101 46-47 0.28632595069042566 1101 48-49 0.22815104494589633 1101 50-51 0.2652270294233716 1101 52-53 0.22522451482298322 1101 54-55 0.27011472880035825 1101 56-57 0.3217530630354588 1101 58-59 0.4821066195490644 1101 60-61 0.35212092626558444 1101 62-63 0.4494867129660065 1101 64-65 0.3581806897522668 1101 66-67 0.3501155828391447 1101 68-69 0.2042443405436032 1101 70-71 0.3373557697556251 1101 72-73 -0.003146239531353956 1101 74-75 0.06656278522311254 1101 76-77 0.302519856218872 1101 78-79 0.3814822800404585 1101 80-81 0.5554105905251134 1101 82-83 0.505841191538071 1101 84-85 0.29255127953504 1101 86-87 0.35550483842436975 1101 88-89 0.3458973306265598 1101 90-91 0.411350966421395 1101 92-93 0.473469669971049 1101 94-95 0.421064810174947 1101 96-97 0.3375513681030995 1101 98-99 0.4208859016364137 1101 100-101 0.3827875686427653 2304 1 -0.013911357422056625 2304 2 -0.1552967814826367 2304 3 -0.09393166552752064 2304 4 -0.0872366906415536 2304 5 -0.008657748328708692 2304 6 -0.11209723973406227 2304 7 0.28937047368977176 2304 8 -0.037474126200990554 2304 9 0.07880805002520219 2304 10-11 -0.03131761865689242 2304 12-13 0.020278113465792558 2304 14-15 0.19359635667998276 2304 16-17 0.1033591921954482 2304 18-19 0.13681542893957754 2304 20-21 0.11042480021036027 2304 22-23 -0.013993349348830009 2304 24-25 0.11878037737066194 2304 26-27 0.02611006816390926 2304 28-29 0.1322083505519558 2304 30-31 0.18696330348215895 2304 32-33 0.16019190623288182 2304 34-35 0.05567039347453573 2304 36-37 0.305809620308203 2304 38-39 0.09718319458650626 2304 40-41 -0.039454792115343196 2304 42-43 0.24926630122370597 2304 44-45 0.3087544272118734 2304 46-47 0.2263227215555048 2304 48-49 0.20120364974631855 2304 50-51 0.11821422637813583 2304 52-53 0.11627928742125704 2304 54-55 0.22574040501366 2304 56-57 0.24425213645776012 2304 58-59 0.3600075369845612 2304 60-61 0.3244896523630487 2304 62-63 0.4205333681905685 2304 64-65 0.35499748606966364 2304 66-67 0.2617581460870966 2304 68-69 0.0616689062535869 2304 70-71 0.2451098878202984 2304 72-73 0.1057852598869502 2304 74-75 0.20172405623763723 2304 76-77 0.34533853765022826 2304 78-79 0.421822999289887 2304 80-81 0.48773150996034786 2304 82-83 0.4518938383789113 2304 84-85 0.40613147545318284 2304 86-87 0.3349501809111253 2304 88-89 0.36058624840484654 2304 90-91 0.37059757567066853 2304 92-93 0.34733925863123716 2304 94-95 0.3672674615404006 2304 96-97 0.35374708500163976 2304 98-99 0.3320242734936656 2304 100-101 0.23925518868565732 2202 1 0.0045435103767204055 2202 2 -0.03493376455313779 2202 3 -0.03195058239972326 2202 4 0.007555583802698607 2202 5 0.07623010359153426 2202 6 0.17008832347722347 2202 7 0.24864548328798008 2202 8 0.02308602450717956 2202 9 0.06472284231703895 2202 10-11 0.08811136678097142 2202 12-13 0.04302602962913227 2202 14-15 0.1282844940956025 2202 16-17 0.12099023222800298 2202 18-19 0.1690410256678092 2202 20-21 0.12397672348733835 2202 22-23 0.17759715447670033 2202 24-25 0.19511181159663238 2202 26-27 0.1187523402352113 2202 28-29 0.24702408813821108 2202 30-31 0.3493795219923541 2202 32-33 0.21186770536205302 2202 34-35 0.06175976713981157 2202 36-37 0.30799783701284156 2202 38-39 0.15078815386398503 2202 40-41 0.1842025244578096 2202 42-43 0.37826369766069234 2202 44-45 0.3633329137513499 2202 46-47 0.29144854670474984 2202 48-49 0.2635161678323428 2202 50-51 0.2722101571696456 2202 52-53 0.2614601558531575 2202 54-55 0.34443311240569585 2202 56-57 0.4355079330798972 2202 58-59 0.33415849348013893 2202 60-61 0.23008297654355658 2202 62-63 0.345171983035911 2202 64-65 0.3168132224202438 2202 66-67 0.3581662918082813 2202 68-69 0.3076130727809385 2202 70-71 0.4377334142745184 2202 72-73 0.37156641734734563 2202 74-75 0.3037901198012918 2202 76-77 0.47739676475877246 2202 78-79 0.5064289804689395 2202 80-81 0.5132295765668786 2202 82-83 0.5151455823597395 2202 84-85 0.3930170033013418 2202 86-87 0.4049033914664655 2202 88-89 0.3867663149765015 2202 90-91 0.44472369971548886 2202 92-93 0.5025790544779198 2202 94-95 0.41035741565162454 2202 96-97 0.36578878704325746 2202 98-99 0.4220942862682371 2202 100-101 0.37599084999336085 1102 1 -0.0789437697222688 1102 2 -0.21877260116436403 1102 3 -0.10553260465569991 1102 4 -0.2225647366599972 1102 5 -0.09768081653582783 1102 6 -0.10138621639397627 1102 7 -0.07480890262413453 1102 8 -0.09111282138925247 1102 9 -0.18250721583515173 1102 10-11 -0.12093701166109128 1102 12-13 -0.1712465514443693 1102 14-15 -0.2501019387726089 1102 16-17 -0.21100093961354105 1102 18-19 -0.15176212327610017 1102 20-21 -0.2475785251426288 1102 22-23 -0.06370846104726269 1102 24-25 -0.17855528835603707 1102 26-27 -0.1408546814971956 1102 28-29 -0.21148783048285935 1102 30-31 -0.1215349226756004 1102 32-33 -0.16945682726718303 1102 34-35 -0.19558106052799218 1102 36-37 -0.12740712965564427 1102 38-39 -0.22176288703437308 1102 40-41 -0.078548856663339 1102 42-43 -0.03651924399933648 1102 44-45 -0.08983673532042502 1102 46-47 -0.19602195086492458 1102 48-49 -0.21081229604908458 1102 50-51 -0.04132603567536819 1102 52-53 -0.10948896313488632 1102 54-55 -0.05210214382349676 1102 56-57 0.162643868557943 1102 58-59 0.04333985394540463 1102 60-61 -0.05798994169052207 1102 62-63 -0.07628084853743644 1102 64-65 0.01766075678174417 1102 66-67 0.05895317580782944 1102 68-69 0.04702790172183313 1102 70-71 0.08147455210610843 1102 72-73 0.04420942152938068 1102 74-75 0.23702122086692157 1102 76-77 0.26104414245447316 1102 78-79 0.1700514257401835 1102 80-81 0.1250659841718047 1102 82-83 0.1219310049429474 1102 84-85 0.08163089548563107 1102 86-87 0.045556354539129984 1102 88-89 0.03272551375991739 1102 90-91 0.08300894481334709 1102 92-93 0.10751053826779611 1102 94-95 0.0967675460263635 1102 96-97 -0.055346449580707 1102 98-99 -0.1277035546580727 1102 100-101 0.029078523182374028 2305 1 0.08852009899769442 2305 2 -0.012138020048972464 2305 3 -0.009136687298450852 2305 4 -0.006779291983363578 2305 5 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-0.2820169777062276 1213 98-99 -0.3637612682114799 1213 100-101 -0.1969094105478355 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 795.0 3 440.0 4 451.0 5 379.0 6 964.0 7 6564.0 8 34818.0 9 104368.0 10 143195.0 11 106353.0 12 62285.0 13 39144.0 14 26417.0 15 19742.0 16 16221.0 17 14734.0 18 14527.0 19 14998.0 20 15768.0 21 16807.0 22 18911.0 23 19411.0 24 21238.0 25 23781.0 26 28429.0 27 34205.0 28 41788.0 29 51474.0 30 62520.0 31 81013.0 32 111420.0 33 160473.0 34 246716.0 35 428443.0 36 888322.0 37 1738261.0 38 727130.0 39 828.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 38.90607985275933 23.70136471736449 24.948452210793935 12.444103219082246 2 29.513421043814205 26.66333865288477 33.43341845368583 10.389821849615188 3 36.55386157958236 20.109424579072176 29.360662441564855 13.976051399780609 4 25.20742843681298 18.217236881619026 38.979931524531274 17.595403157036724 5 33.572736830596526 17.4341334588875 33.79612614957242 15.197003560943545 6 28.365816628941765 17.483776222381863 40.75709770872236 13.393309439954013 7 35.99144457121104 18.732454393177324 30.557577426367228 14.718523609244407 8 25.71928685562279 32.65898975977756 27.921664404770148 13.7000589798295 9 34.25415164627278 26.68915729713771 24.0865249907386 14.970166065850913 10-11 26.935557639471565 30.341434752668157 28.12162235211443 14.60138525574585 12-13 29.745788125645266 24.265750725421594 27.502404750125564 18.48605639880758 14-15 25.802330221832214 25.12079525139066 29.537184739063072 19.539689787714053 16-17 27.001969634457357 26.245858671487767 30.58524131877125 16.166930375283624 18-19 26.07410129077466 26.93454251534692 30.861209358142133 16.13014683573628 20-21 24.782491740397976 29.093073649446122 29.816949411342048 16.307485198813858 22-23 27.853396289951664 26.916154171139194 28.931352580189124 16.299096958720018 24-25 23.97365141903997 30.742922058307474 28.841431793085476 16.441994729567075 26-27 21.896562335837093 33.03270562677268 28.438769953880517 16.63196208350971 28-29 23.329317185259306 31.109701573498988 28.414388640167232 17.14659260107447 30-31 25.844459565448442 28.0374557221375 28.798948396061245 17.319136316352814 32-33 22.091045809020894 30.241739165953806 30.640259756027433 17.026955268997867 34-35 26.617393497673437 28.077198186938123 28.8371331335552 16.468275181833242 36-37 23.223040905498447 29.583313606938972 30.80362365785613 16.390021829706452 38-39 22.185998596631258 32.359687832811815 28.6816475417281 16.772666028828827 40-41 27.09571823737415 27.69557724688301 27.983610090702626 17.225094425040215 42-43 26.77074392547435 27.799282208056493 28.702744406688268 16.72722945978089 44-45 23.603278212693052 29.779272734322955 29.838754762562235 16.778694290421754 46-47 24.010123303035908 30.400559539829324 28.673535714672344 16.91578144246243 48-49 20.730892331857827 30.385553159759205 30.378371418212296 18.50518309017067 50-51 19.21808617533925 28.35676106630346 32.297514873437535 20.127637884919764 52-53 24.718273921045355 27.846047533469708 28.664067310438092 18.771611235046848 54-55 26.181548429012423 27.704354860141056 28.38178925944454 17.732307451401976 56-57 26.638492166716045 27.54700779733333 28.040924236030357 17.77357579992027 58-59 25.26513255828004 27.596390579731555 28.39590543895585 18.742571423032555 60-61 21.39315681223291 28.032729352921145 27.916841513005746 22.657272321840203 62-63 23.902090894363614 28.113250396486645 28.28620117343866 19.698457535711082 64-65 21.335647601084414 29.428073928116078 30.652434967432914 18.583843503366595 66-67 19.593827356114097 29.356020759148933 29.498769076862217 21.55138280787475 68-69 18.743766208094172 28.264526446331455 34.52852469331931 18.46318265225506 70-71 18.105637732954957 32.326968039688005 31.776123149315083 17.791271078041955 72-73 17.882365072304403 36.32868978992473 28.110579481401366 17.678365656369508 74-75 18.291684254523087 36.22486316279251 27.675909059736497 17.807543522947906 76-77 18.74995589842874 35.816722995937575 27.839273270082348 17.594047835551333 78-79 18.736798609927906 35.574036495507755 28.175189802249278 17.51397509231506 80-81 18.151075777998273 35.69873586982314 28.020997142210362 18.129191209968223 82-83 17.816708051649588 35.23770131947278 28.145402220154935 18.800188408722704 84-85 18.055158991945238 34.17523604814978 28.832892120372556 18.93671283953243 86-87 18.10122178042586 33.75542877320619 29.49325301379438 18.65009643257357 88-89 18.514818264086376 33.952900240425905 28.917462243156283 18.614819252331436 90-91 18.958348840424062 33.25411582105305 28.739479840656585 19.048055497866304 92-93 18.72070736606958 33.661531259149534 28.615633977837135 19.002127396943745 94-95 18.439076076556194 33.936826255644306 28.506725941837903 19.117371725961597 96-97 19.07011549388488 33.875728482011816 28.185378101194193 18.868777922909107 98-99 19.43689951719053 32.98234717581634 27.94710693093439 19.633646376058746 100-101 19.394932234883683 32.987164330842354 27.67046171647158 19.94744171780238 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 563.0 1 586.0 2 610.5 3 805.5 4 973.0 5 922.0 6 973.5 7 1132.5 8 1358.0 9 1600.0 10 1835.0 11 1993.5 12 2060.5 13 2081.5 14 2033.0 15 2054.0 16 2142.0 17 2317.5 18 2759.5 19 3445.5 20 4422.5 21 6034.0 22 8426.5 23 11983.5 24 16655.0 25 22641.0 26 30882.0 27 41470.0 28 53290.5 29 65384.5 30 78277.0 31 91790.0 32 107551.0 33 127859.5 34 153214.5 35 188654.5 36 238017.0 37 296504.5 38 349308.5 39 363228.0 40 353235.5 41 319865.0 42 271144.0 43 255695.0 44 240751.5 45 223247.0 46 229007.5 47 209974.0 48 156049.0 49 114821.0 50 92784.5 51 80126.0 52 70793.5 53 60671.0 54 52921.5 55 46593.5 56 40597.5 57 35119.5 58 30259.5 59 25977.0 60 22106.0 61 18757.5 62 15902.0 63 13424.5 64 11206.0 65 9246.0 66 7531.0 67 6122.5 68 5004.0 69 3991.0 70 3170.0 71 2488.5 72 1868.0 73 1409.0 74 1067.5 75 773.5 76 548.5 77 406.0 78 296.5 79 200.0 80 135.5 81 102.5 82 84.5 83 61.5 84 47.5 85 42.5 86 34.5 87 28.0 88 23.0 89 19.0 90 11.0 91 5.5 92 5.0 93 5.5 94 7.5 95 5.0 96 1.5 97 1.0 98 1.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22085787231420614 2 0.2039136007459988 3 0.17913589099160243 4 0.16397621565286258 5 0.17455229646539114 6 0.13555417254565888 7 0.12163432195581227 8 0.11761428413364335 9 0.10450219815292412 10-11 0.09055416972787537 12-13 0.11745460973416467 14-15 0.1140450916747083 16-17 0.10510332530390264 18-19 0.09857546014874516 20-21 0.1020319412668717 22-23 0.0892298114733758 24-25 0.4037226301642223 26-27 0.19525361272721434 28-29 0.09024421354065207 30-31 0.1721665730849451 32-33 0.2030588730782012 34-35 0.20237321242161632 36-37 0.20807452774417834 38-39 0.2088071514594334 40-41 0.20509706982448778 42-43 0.22102693932541886 44-45 0.20295555434912677 46-47 0.22214466012176956 48-49 0.21135254923935815 50-51 0.21472449685187833 52-53 0.20648717636112565 54-55 0.18881028107766318 56-57 0.19707577940361798 58-59 0.19228554741925782 60-61 0.17175329816864734 62-63 0.16016281528884255 64-65 0.16465248369771343 66-67 0.17723858342132645 68-69 0.1700344502213181 70-71 0.16303695447945865 72-73 0.16638072425677672 74-75 0.15684722334672657 76-77 0.16712274058376586 78-79 0.1650939364492133 80-81 0.17068254043096684 82-83 0.195657495031778 84-85 0.1940137879783211 86-87 0.19824985587037297 88-89 0.20448655006177519 90-91 0.18603946061612153 92-93 0.16638072425677672 94-95 0.1673763411005849 96-97 0.17605511434283747 98-99 0.19086726304741786 100-101 0.20202568578745686 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5323333.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.19234123907932 #Duplication Level Percentage of deduplicated Percentage of total 1 94.09031564362449 58.51697017800978 2 3.156604139082693 3.9263320354904216 3 0.9800347853549088 1.8285197339088104 4 0.3563971567629573 0.8866069436015795 5 0.21584613443409634 0.6711988223930755 6 0.17086833682699556 0.6376021146539073 7 0.14134582392776918 0.6153439400104251 8 0.10315068918022462 0.513214628843419 9 0.06933431973422441 0.38808573052412854 >10 0.5519746747587247 6.682400110878686 >50 0.07430717751901343 3.2234995481237863 >100 0.06937335229018664 9.119733975128641 >500 0.014159558340980325 6.170387260673634 >1k 0.006074962590587954 5.766918376165417 >5k 1.8278252104600838E-4 0.856378830020262 >10k+ 3.046305094428827E-5 0.19680777157396462 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT 10388 0.19514090138640583 Illumina Single End PCR Primer 1 (97% over 34bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 9428 0.1771070868570499 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 8507 0.15980589604294904 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 8245 0.1548841674943123 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 6974 0.13100814846638376 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 6613 0.1242266827944072 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 5434 0.1020789043255419 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0017470257825313578 0.0 0.0 2 0.001934878017212149 0.0 0.0 3 0.00212273025189294 0.0 0.0 4 0.0023105824865737312 0.0 1.8785223468079115E-5 5 0.0026675017324672345 0.0 1.8785223468079115E-5 6 0.0028741391906161044 0.0 1.8785223468079115E-5 7 0.0032122732130415287 0.0 1.8785223468079115E-5 8 0.0037946151405519814 0.0 1.8785223468079115E-5 9 0.004038823045637009 0.0 1.8785223468079115E-5 10-11 0.007147777529604103 0.0 2.8177835202118672E-5 12-13 0.009618034415656507 0.0 5.6355670404237344E-5 14-15 0.012069506078240831 0.0 5.6355670404237344E-5 16-17 0.013234189933261736 0.0 5.6355670404237344E-5 18-19 0.014868504374984621 0.0 7.514089387231646E-5 20-21 0.01635253702896287 0.0 9.392611734039557E-5 22-23 0.017939888412015555 0.0 1.314965642765538E-4 24-25 0.019658736359344793 0.0 1.314965642765538E-4 26-27 0.021546651317886745 0.0 1.314965642765538E-4 28-29 0.023932374698332794 0.0 1.5028178774463292E-4 30-31 0.04095178716041247 0.0 1.5028178774463292E-4 32-33 0.044013778585709366 0.0 1.6906701121271203E-4 34-35 0.04633375368401714 0.0 1.784596229467516E-4 36-37 0.04828741692469737 0.0 1.8785223468079115E-4 38-39 0.050701318140345536 0.0 1.8785223468079115E-4 40-41 0.05350031643708932 0.0 2.0663745814887026E-4 42-43 0.05667501920319469 0.0 2.442079050850285E-4 44-45 0.06048841956721475 0.0 2.442079050850285E-4 46-47 0.06452724261285175 0.0 2.442079050850285E-4 48-49 0.06868816961103129 0.0 2.442079050850285E-4 50-51 0.07375078733567861 0.0 2.442079050850285E-4 52-53 0.07888854595419825 0.0 2.629931285531076E-4 54-55 0.08497495835785587 0.0 2.817783520211867E-4 56-57 0.0918221723119707 0.0 2.817783520211867E-4 58-59 0.0998904257915107 0.0 2.817783520211867E-4 60-61 0.10867251776283768 0.0 3.0056357548926584E-4 62-63 0.11862868620091961 0.0 3.1934879895734495E-4 64-65 0.12935504880119278 0.0 3.1934879895734495E-4 66-67 0.14118034697434861 0.0 3.1934879895734495E-4 68-69 0.15465874481269537 0.0 3.1934879895734495E-4 70-71 0.170485295584552 0.0 3.6631185762754274E-4 72-73 0.18909205942968438 0.0 3.757044693615823E-4 74-75 0.20984033875017777 0.0 3.944896928296614E-4 76-77 0.23253288869961733 0.0 3.944896928296614E-4 78-79 0.2569912496550564 0.0 4.132749162977405E-4 80-81 0.2857420341729514 0.0 4.3206013976581964E-4 82-83 0.3182498633844623 0.0 4.3206013976581964E-4 84-85 0.3544302037839827 0.0 4.3206013976581964E-4 86-87 0.3959267624249695 0.0 4.5084536323389875E-4 88-89 0.4410112987483593 0.0 4.602379749679383E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 5415 0.0 88.068306 9 CGGGAGA 8145 0.0 87.13531 4 TCGGGAG 8340 0.0 84.870285 3 AGAGCGG 5850 0.0 83.95306 8 GAGAGCG 7355 0.0 82.90498 7 GTTCGGA 1130 0.0 82.78008 2 AAGCGGC 1675 0.0 81.87193 9 TCGGAGA 2025 0.0 79.9417 4 CGGAGAG 3115 0.0 79.09579 5 ATTCGGA 1605 0.0 78.10287 2 CGGAAAG 3060 0.0 77.569786 5 ATCCGGA 1350 0.0 77.02796 2 ATCGGGA 10600 0.0 76.7791 2 GATCGGG 11045 0.0 75.37935 1 TTCGGAG 1500 0.0 75.00688 3 GATCCGG 1485 0.0 73.87912 1 AGAGCGT 11775 0.0 73.786934 8 GAGCGTC 11765 0.0 73.163994 9 AAAGCGG 2050 0.0 70.59891 8 CCGGAGA 1360 0.0 69.114685 4 >>END_MODULE