FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n02_52260024-1.3420000003582d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n02_52260024-1.3420000003582d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6634688
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA77600.11696103871048646No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC71180.10728462287902611No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG69900.1053553686322552No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT66730.10057744991173662Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC24800.079.387039
CGGGAGA37550.075.8410344
AGAGCGG27950.074.174758
TCGGGAG41450.065.844473
ATTCGGA4050.064.469722
CGGAAAG15200.063.3892145
AAGCGGC10950.063.254579
TCGGGAA14000.062.382943
GAGAGCG37550.061.909277
ACCGGAA4900.056.1833883
AACCGGA5150.055.3085372
GATCCGG7750.055.141361
TCGGAGA10050.052.4227564
AAGCGTC4850.051.8426679
TTGTGTT687800.049.3537141
CGGAGAG18600.048.7397545
GATCGGG73600.048.2570151
GAACCGG6050.046.305581
GTTCGGA5700.045.8074342
ATCGGGA74900.044.9378782
Produced by FastQC (version 0.11.2)