##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l07n02_52150223-1.3420000003573b.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3230161 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.58757597531516 33.0 33.0 33.0 27.0 33.0 2 31.33905461678226 33.0 33.0 33.0 27.0 33.0 3 31.25614110256424 33.0 33.0 33.0 27.0 33.0 4 34.812986101931145 37.0 37.0 37.0 33.0 37.0 5 34.813592882831536 37.0 37.0 37.0 33.0 37.0 6 35.13842963245485 37.0 37.0 37.0 33.0 37.0 7 34.76646829678149 37.0 37.0 37.0 33.0 37.0 8 34.94468387179462 37.0 37.0 37.0 33.0 37.0 9 34.62244854049071 37.0 37.0 37.0 33.0 37.0 10-11 34.616085854544096 37.0 37.0 37.0 33.0 37.0 12-13 34.59525918367537 37.0 37.0 37.0 33.0 37.0 14-15 36.22079162617591 40.0 37.0 40.0 33.0 40.0 16-17 36.163831771852855 40.0 37.0 40.0 30.0 40.0 18-19 36.14750828209492 40.0 37.0 40.0 27.0 40.0 20-21 36.09817235116144 40.0 37.0 40.0 27.0 40.0 22-23 36.49287837355475 40.0 37.0 40.0 33.0 40.0 24-25 36.05598234886744 40.0 37.0 40.0 27.0 40.0 26-27 35.96187960909688 40.0 37.0 40.0 27.0 40.0 28-29 35.75155603699011 40.0 37.0 40.0 27.0 40.0 30-31 35.70803297420778 40.0 37.0 40.0 27.0 40.0 32-33 35.62218570529457 40.0 37.0 40.0 27.0 40.0 34-35 35.619160004718026 38.5 37.0 40.0 27.0 40.0 36-37 35.51021218447006 37.0 37.0 40.0 27.0 40.0 38-39 35.3889713237204 37.0 37.0 40.0 27.0 40.0 40-41 35.334874175002426 37.0 37.0 40.0 27.0 40.0 42-43 35.23874939979772 37.0 37.0 40.0 27.0 40.0 44-45 35.08217283906282 37.0 37.0 40.0 22.0 40.0 46-47 34.973602244594 37.0 37.0 40.0 22.0 40.0 48-49 34.867962463790505 37.0 37.0 40.0 22.0 40.0 50-51 33.87660506705393 37.0 35.0 38.5 22.0 38.5 52-53 34.29253402539378 37.0 35.0 38.5 22.0 40.0 54-55 34.980804981547365 37.0 37.0 40.0 22.0 40.0 56-57 35.15323059748415 37.0 37.0 40.0 24.5 40.0 58-59 34.812204561939794 37.0 37.0 40.0 22.0 40.0 60-61 34.693061119863685 37.0 35.0 40.0 22.0 40.0 62-63 34.43310735904495 37.0 33.0 40.0 22.0 40.0 64-65 34.253059212838 37.0 33.0 40.0 22.0 40.0 66-67 34.069541889707665 37.0 33.0 40.0 22.0 40.0 68-69 34.01620615814505 37.0 33.0 40.0 22.0 40.0 70-71 33.655185453604325 37.0 33.0 37.0 22.0 40.0 72-73 33.69872445986438 37.0 33.0 37.0 22.0 40.0 74-75 33.58016968813629 37.0 33.0 37.0 24.5 40.0 76-77 33.26339584930906 37.0 33.0 37.0 22.0 40.0 78-79 32.85520427619552 37.0 33.0 37.0 22.0 37.0 80-81 32.426083715331835 37.0 33.0 37.0 22.0 37.0 82-83 32.09246737236936 37.0 33.0 37.0 15.0 37.0 84-85 31.843432726727862 37.0 33.0 37.0 15.0 37.0 86-87 31.637781522345172 35.0 33.0 37.0 15.0 37.0 88-89 31.405755316840242 33.0 33.0 37.0 15.0 37.0 90-91 31.233512818710892 33.0 33.0 37.0 15.0 37.0 92-93 31.03291229136876 33.0 33.0 37.0 15.0 37.0 94-95 30.87224444849653 33.0 33.0 37.0 15.0 37.0 96-97 30.729148485168388 33.0 27.0 37.0 15.0 37.0 98-99 30.566129830680268 33.0 27.0 37.0 15.0 37.0 100-101 29.280584311432158 33.0 27.0 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.12257348244625277 2306 2 0.13624386156971724 2306 3 0.14149602485310453 2306 4 0.23562040424701536 2306 5 0.2801764867630183 2306 6 0.08279539316156104 2306 7 0.26327362988634917 2306 8 0.01075147798088949 2306 9 0.14878264972474398 2306 10-11 0.11548724104609676 2306 12-13 0.1337292421087568 2306 14-15 0.32116227920433715 2306 16-17 0.27547597601675733 2306 18-19 0.3219711189379524 2306 20-21 0.2767316937239954 2306 22-23 0.1528135621475144 2306 24-25 0.20107419517913883 2306 26-27 0.11435807337350923 2306 28-29 0.26753340809526804 2306 30-31 0.2742678906443672 2306 32-33 0.09659463688711867 2306 34-35 0.23851388070281843 2306 36-37 0.14533068419170547 2306 38-39 0.18325973992547517 2306 40-41 0.257654401955989 2306 42-43 0.1463963211270638 2306 44-45 0.3005282509314213 2306 46-47 0.3218970155616603 2306 48-49 0.4892414598215211 2306 50-51 0.4639441273364113 2306 52-53 0.34175241287739055 2306 54-55 0.37173181249460185 2306 56-57 0.19791205611976892 2306 58-59 0.3945865342757955 2306 60-61 0.3102437379494276 2306 62-63 0.37828691492610034 2306 64-65 0.35088446288908415 2306 66-67 0.293309427176915 2306 68-69 0.2076292433605289 2306 70-71 0.49879250555165555 2306 72-73 0.184807688374498 2306 74-75 0.13055328725629778 2306 76-77 0.2118484825956486 2306 78-79 0.09150943361632358 2306 80-81 0.16124371534942128 2306 82-83 0.19649353686087068 2306 84-85 0.2948149060427028 2306 86-87 0.31902165115945635 2306 88-89 0.31952644019051846 2306 90-91 0.3503556428522323 2306 92-93 0.24103803346636 2306 94-95 0.1867235576358368 2306 96-97 0.08141015450746636 2306 98-99 0.12904492932412737 2306 100-101 0.06983106374554637 2307 1 0.24970300706380755 2307 2 0.2972648386533585 2307 3 0.3431764424901296 2307 4 0.30789178173929344 2307 5 0.48098336426827615 2307 6 0.1883292569100803 2307 7 0.5982736731737717 2307 8 0.28635382242755014 2307 9 0.5434890311561773 2307 10-11 0.48327793771382943 2307 12-13 0.4364269141113155 2307 14-15 0.6265524287190871 2307 16-17 0.6345925230658551 2307 18-19 0.6692121952363692 2307 20-21 0.6230180659784921 2307 22-23 0.358362576492965 2307 24-25 0.5494740757058594 2307 26-27 0.5130605329647082 2307 28-29 0.5741137452176943 2307 30-31 0.5558369730376427 2307 32-33 0.6393074161991947 2307 34-35 0.5478992426289366 2307 36-37 0.5016300859595617 2307 38-39 0.45512508141992924 2307 40-41 0.665464534675209 2307 42-43 0.548609174027753 2307 44-45 0.5367269228733846 2307 46-47 0.4546616964900849 2307 48-49 0.48516508349800347 2307 50-51 0.4081148269476955 2307 52-53 0.2380398673173758 2307 54-55 0.42406716879335704 2307 56-57 0.3547235480639799 2307 58-59 0.5318760491969741 2307 60-61 0.4524004903540444 2307 62-63 0.5463016239913543 2307 64-65 0.6100085852366419 2307 66-67 0.584015185269493 2307 68-69 0.36349165862539934 2307 70-71 0.33253028767741455 2307 72-73 0.2271239549211188 2307 74-75 0.1657689018943742 2307 76-77 0.27136284103279706 2307 78-79 0.28748695013047154 2307 80-81 0.44370062272299293 2307 82-83 0.3933725138925652 2307 84-85 0.5988953946711035 2307 86-87 0.44683100470513537 2307 88-89 0.4399604831489512 2307 90-91 0.49197519837700554 2307 92-93 0.4827848540053772 2307 94-95 0.43203293282521926 2307 96-97 0.41089841063047317 2307 98-99 0.45511521077893136 2307 100-101 0.35536332585953545 2201 1 0.030176987127937593 2201 2 -0.07538534745354042 2201 3 0.017958920268355882 2201 4 0.12959250216752594 2201 5 0.038749544835944505 2201 6 0.08969110924390833 2201 7 0.2679160378717427 2201 8 -0.0050693102800138945 2201 9 0.25161087937582494 2201 10-11 0.19866034006546585 2201 12-13 0.12874756355508765 2201 14-15 0.2698860452747667 2201 16-17 0.2234399347861924 2201 18-19 0.24157569324113126 2201 20-21 0.33067446061753714 2201 22-23 0.08514579661189003 2201 24-25 0.2206292577060367 2201 26-27 0.1764569130848841 2201 28-29 0.23411630167210973 2201 30-31 0.3549018920733147 2201 32-33 0.32741979137639277 2201 34-35 0.2432699359721724 2201 36-37 0.3480801546037142 2201 38-39 0.29734824274785865 2201 40-41 0.27535350405887016 2201 42-43 0.3497106853916847 2201 44-45 0.342633525084608 2201 46-47 0.2533628983963254 2201 48-49 0.3364126034390935 2201 50-51 0.15191840628200026 2201 52-53 0.2854619103641909 2201 54-55 0.25368842597266905 2201 56-57 0.4424853983109074 2201 58-59 0.3749913609428006 2201 60-61 0.35183195874270723 2201 62-63 0.3874904647324371 2201 64-65 0.3717447978978754 2201 66-67 0.3625185340011825 2201 68-69 0.2634634854543094 2201 70-71 0.31563570154726506 2201 72-73 0.19028511142233384 2201 74-75 0.24895913340628795 2201 76-77 0.3570023869230212 2201 78-79 0.29417826459523866 2201 80-81 0.5217876345929895 2201 82-83 0.39092189520078335 2201 84-85 0.3796818509780877 2201 86-87 0.3157104205515715 2201 88-89 0.38744155569710514 2201 90-91 0.33407128230622263 2201 92-93 0.3516350159638435 2201 94-95 0.19383361583828673 2201 96-97 0.28605548723913543 2201 98-99 0.2853983138823004 2201 100-101 -0.01590745558075568 1101 1 0.25307322592155757 1101 2 0.2459183880418152 1101 3 0.17878387058791034 1101 4 0.39424401369547013 1101 5 0.430603819365011 1101 6 0.27262825585202677 1101 7 0.5010298938857147 1101 8 0.30576113415437334 1101 9 0.42196434846047737 1101 10-11 0.3807602625733111 1101 12-13 0.41282310512039544 1101 14-15 0.6275144980386713 1101 16-17 0.5916266039808704 1101 18-19 0.6190761439021912 1101 20-21 0.6240828155502811 1101 22-23 0.5351965722875391 1101 24-25 0.6112665678448721 1101 26-27 0.4299551919546545 1101 28-29 0.5198325367455325 1101 30-31 0.5394896072325537 1101 32-33 0.5750632470238131 1101 34-35 0.4517159345688313 1101 36-37 0.6096458049668669 1101 38-39 0.5592108625859851 1101 40-41 0.6493256916286754 1101 42-43 0.5166797079204244 1101 44-45 0.6131719986112358 1101 46-47 0.6586951702523081 1101 48-49 0.6200486158177299 1101 50-51 0.5677534147817198 1101 52-53 0.6161176706742779 1101 54-55 0.6674751710076805 1101 56-57 0.6636651750846596 1101 58-59 0.7395115164442032 1101 60-61 0.7094166022249269 1101 62-63 0.7530559384220936 1101 64-65 0.6208249825288661 1101 66-67 0.5934952511634322 1101 68-69 0.5175905352439187 1101 70-71 0.7133825040083437 1101 72-73 0.5247041608471079 1101 74-75 0.5597455890127563 1101 76-77 0.6795724620236143 1101 78-79 0.5897882154785634 1101 80-81 0.6981259183751618 1101 82-83 0.6541944916332696 1101 84-85 0.49755054378812247 1101 86-87 0.6139760398173131 1101 88-89 0.5079361122143062 1101 90-91 0.5362482445131782 1101 92-93 0.6007108914851571 1101 94-95 0.5467457480647511 1101 96-97 0.5141560532406935 1101 98-99 0.6854411536143061 1101 100-101 0.6573900331419438 2304 1 0.06019980919451484 2304 2 0.0030768008519999057 2304 3 0.09018944937304596 2304 4 0.08109050997308032 2304 5 0.21917211740457532 2304 6 0.30622967503878584 2304 7 0.38814285948362937 2304 8 0.257948448960299 2304 9 0.24033246473705816 2304 10-11 0.2559173491797182 2304 12-13 0.2825713527176603 2304 14-15 0.47230145352160946 2304 16-17 0.3457150587336244 2304 18-19 0.3586750464701822 2304 20-21 0.29248903351261646 2304 22-23 0.30571684377458297 2304 24-25 0.4307161447819823 2304 26-27 0.4091436211985169 2304 28-29 0.3975294393710769 2304 30-31 0.361631924222408 2304 32-33 0.4470010079120499 2304 34-35 0.42532473807891336 2304 36-37 0.36421372344445757 2304 38-39 0.3702855855364575 2304 40-41 0.4648458806725273 2304 42-43 0.3251208559305212 2304 44-45 0.37538450223630804 2304 46-47 0.21800108265755114 2304 48-49 0.2978001734282216 2304 50-51 0.3800873362098045 2304 52-53 0.2653993318495793 2304 54-55 0.38824170515096057 2304 56-57 0.4156002460617785 2304 58-59 0.4570946927313386 2304 60-61 0.42032147603996606 2304 62-63 0.4473925601071116 2304 64-65 0.4017610618993501 2304 66-67 0.5478458105970461 2304 68-69 0.45105912390381064 2304 70-71 0.4651435551945653 2304 72-73 0.4235635027435407 2304 74-75 0.34906945463488626 2304 76-77 0.44655422332697725 2304 78-79 0.414950862958932 2304 80-81 0.45352473487892553 2304 82-83 0.365226667431898 2304 84-85 0.44730570368103173 2304 86-87 0.40991483981304455 2304 88-89 0.5591411729975668 2304 90-91 0.3596972860545229 2304 92-93 0.440294226839157 2304 94-95 0.3215519338257451 2304 96-97 0.25024918042079136 2304 98-99 0.3927589404252707 2304 100-101 0.39439060119168445 2202 1 0.15732011412105962 2202 2 -0.02748004690648642 2202 3 0.03531042457775868 2202 4 -0.004898091963390527 2202 5 0.20562732965099428 2202 6 0.11917079560382149 2202 7 0.23278338530184328 2202 8 0.18696023885310353 2202 9 0.07859492699222415 2202 10-11 0.06145206275252235 2202 12-13 0.09933593501931881 2202 14-15 0.2793636760106111 2202 16-17 0.25702762845005367 2202 18-19 0.18579559316372496 2202 20-21 0.18947253166435019 2202 22-23 0.2891826904342807 2202 24-25 0.23948636775784848 2202 26-27 0.2267247923845659 2202 28-29 0.38568848370780273 2202 30-31 0.4416478391604386 2202 32-33 0.32652809498651436 2202 34-35 0.17815758037513518 2202 36-37 0.22726918312689293 2202 38-39 0.17431404901547154 2202 40-41 0.21892153040049322 2202 42-43 0.3406608466933605 2202 44-45 0.25626444241147794 2202 46-47 0.3555264890345313 2202 48-49 0.32329596251403103 2202 50-51 0.2003075192524335 2202 52-53 0.1943477621148091 2202 54-55 0.22012527016377703 2202 56-57 0.3202498423108864 2202 58-59 0.3432115979136583 2202 60-61 0.3889336584289893 2202 62-63 0.4596887046737663 2202 64-65 0.4437684949840772 2202 66-67 0.3237751129241815 2202 68-69 0.4617673178042807 2202 70-71 0.4484049420034282 2202 72-73 0.47532331639953895 2202 74-75 0.3962723771810772 2202 76-77 0.5060291137398352 2202 78-79 0.4328222379128661 2202 80-81 0.4161017043212425 2202 82-83 0.4769344845093215 2202 84-85 0.5726660615628845 2202 86-87 0.32820342957780113 2202 88-89 0.28947717910677184 2202 90-91 0.2048294742459582 2202 92-93 0.4407648388097911 2202 94-95 0.47873153836162885 2202 96-97 0.2663756645783799 2202 98-99 0.3513065293413362 2202 100-101 0.34118685497033496 1102 1 0.08375150243135465 1102 2 -0.0639371342310362 1102 3 -0.00340316804236096 1102 4 -0.023748377395762077 1102 5 -0.028386809591516737 1102 6 0.2004530987968991 1102 7 0.08983647285180041 1102 8 0.07062222610885982 1102 9 0.10939573251862811 1102 10-11 0.13982236034859596 1102 12-13 0.08919707847127967 1102 14-15 0.025090032013729058 1102 16-17 -0.007967406121871079 1102 18-19 0.09341138492622036 1102 20-21 0.019644790551346603 1102 22-23 0.11341225163716473 1102 24-25 -0.012896665576441535 1102 26-27 0.10486406998388276 1102 28-29 0.001963631155504686 1102 30-31 0.10750868702589145 1102 32-33 0.19857209697268985 1102 34-35 0.04246556238050658 1102 36-37 0.07003643107825752 1102 38-39 0.022459004427631157 1102 40-41 0.1051998038350277 1102 42-43 0.2067884667009494 1102 44-45 0.04045239821810753 1102 46-47 0.030758253313855732 1102 48-49 -0.06220506725409081 1102 50-51 0.05813373033310398 1102 52-53 0.06590239830416778 1102 54-55 0.31252981326464635 1102 56-57 0.35831085859685885 1102 58-59 0.2755310588829545 1102 60-61 0.3056004580032621 1102 62-63 0.20970983698276058 1102 64-65 0.1846018575570838 1102 66-67 0.3655738897489229 1102 68-69 0.2375572408560913 1102 70-71 0.36636798943656146 1102 72-73 0.4937148047839415 1102 74-75 0.4455890726089464 1102 76-77 0.4347960873699108 1102 78-79 0.32555769436542903 1102 80-81 0.25062163674038374 1102 82-83 0.38881740779611107 1102 84-85 0.27597694946010876 1102 86-87 0.28660972918556027 1102 88-89 0.24838879342378917 1102 90-91 0.3530590652201475 1102 92-93 0.33196215556476005 1102 94-95 0.27197581828418294 1102 96-97 0.06179909757115709 1102 98-99 0.16400050798893417 1102 100-101 0.25992289302656957 2305 1 0.05322780833159513 2305 2 0.04318320827466238 2305 3 0.02171224883640832 2305 4 0.013951150097938125 2305 5 0.059484957357867074 2305 6 0.0917236131714958 2305 7 0.012802104167192851 2305 8 0.19471523031977966 2305 9 0.031130188700977612 2305 10-11 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13 14481.0 14 10896.0 15 9359.0 16 8742.0 17 9084.0 18 9526.0 19 10637.0 20 11566.0 21 12831.0 22 14483.0 23 15931.0 24 18052.0 25 20845.0 26 24679.0 27 29603.0 28 35588.0 29 44194.0 30 54040.0 31 68615.0 32 91622.0 33 129008.0 34 191666.0 35 309057.0 36 547142.0 37 936521.0 38 450142.0 39 1385.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.40332938470235 25.583219414605633 20.86959030833451 16.143860892357505 2 30.742755606953775 26.617283429772204 29.1163287609531 13.523632202320924 3 35.928027272152114 22.09430130617035 25.985892516657426 15.991778905020116 4 29.586955119618175 21.531457446491828 30.678607398366726 18.20298003552327 5 33.05755316479917 19.810398291357952 29.983355811929048 17.14869273191383 6 29.789659256861967 20.21094523291611 33.299822899994766 16.69957261022716 7 33.66946688417092 21.127255178305035 26.834138332583763 18.369139604940276 8 28.742295968100617 29.68464803596584 24.077523055918448 17.495532940015092 9 30.911612971004992 26.784424306828104 24.22671770179531 18.077245020371592 10-11 26.9851460697414 29.952198343255994 27.655367004059748 15.407288582942863 12-13 27.868692151826153 27.03921956057665 27.470584360991978 17.62150392660522 14-15 25.745757330114603 27.200816479471555 28.54832993469311 18.505096255720726 16-17 26.220581736457728 27.60750360571485 28.900912414753588 17.271002243073834 18-19 25.637135043267932 27.83084578810295 29.269701508517006 17.26231766011211 20-21 24.641804108927438 29.074013902814787 28.702403436362538 17.581778551895233 22-23 26.236236326183576 28.139756752938027 27.9714445598475 17.652562361030892 24-25 23.43344713822935 30.480279249145685 28.2360375815711 17.85023603105386 26-27 22.7742059359885 31.028293391673934 28.148377689001304 18.049122983336265 28-29 23.407252085917456 30.39880554170642 28.04182029395424 18.152122078421883 30-31 24.748091311869647 28.493627678935685 28.50683890047283 18.25144210872184 32-33 24.67033102538812 28.431931695699912 28.629600463708844 18.268136815203125 34-35 22.529816847453326 28.439271410166967 28.284816619447156 20.74609512293255 36-37 21.07445118430894 28.37242871825481 28.713787533260266 21.839332564175983 38-39 20.745951479803935 29.54276540299063 29.779859775392442 19.931423341812994 40-41 21.631868197492192 28.521187985876793 31.007298146930523 18.83964566970049 42-43 22.501545538107113 28.316709264308347 30.410217228580628 18.771527969003913 44-45 21.421995929017932 29.417723615899444 30.360427560142224 18.799852894940404 46-47 22.68951105581351 30.002047803807987 28.29231778812367 19.016123352254834 48-49 20.759377349601827 29.535717515559824 29.820830329798586 19.884074805039763 50-51 20.380542612017237 28.425592433824036 30.640667140938454 20.55319781322027 52-53 23.442132540148616 28.36516402711247 28.41461398167994 19.77808945105897 54-55 23.900814844212476 28.310691362148866 28.714678284566908 19.073815509071753 56-57 24.20686355328044 28.273735621442924 28.40598771468476 19.11341311059188 58-59 23.152694156187874 28.604159238494987 28.21976831703775 20.02337828827939 60-61 21.1014830471352 28.396887807529964 28.150108689803687 22.351520455531148 62-63 22.563411783986044 28.50802669917627 28.351509902575078 20.577051614262608 64-65 20.94007969899141 29.441305449694916 29.843816211353502 19.77479863996017 66-67 20.314064729582164 29.29741325437702 28.857718911338203 21.530803104702613 68-69 20.077978422196793 28.631927581940143 31.589990727560853 19.700103268302215 70-71 20.004068454853872 30.621076611764746 29.871699477628383 19.503155455752996 72-73 19.927429155666974 32.48858579885143 28.070298392631155 19.513686652850442 74-75 19.789161193579087 32.61248059316166 28.183473420189557 19.414884793069692 76-77 19.89888966243319 32.54701607438843 27.987608130381325 19.566486132797056 78-79 19.772165849344354 32.53027048851418 28.09851157823316 19.59905208390831 80-81 19.768553413869707 32.17154685008645 28.197086232863512 19.86281350318033 82-83 19.88333026759406 32.06429305034647 28.017191507788475 20.035185174270993 84-85 19.918275329351477 31.636197884315248 28.313006077936993 20.13252070839628 86-87 20.098658394726638 31.32285381930981 28.306971694455214 20.27151609150834 88-89 20.17809668282907 31.39494591333104 28.187452885501298 20.23950451833859 90-91 20.27283498391804 31.0537946906278 28.227805502913984 20.445564822540174 92-93 20.26924901774113 31.065460149904954 28.1179237822936 20.547367050060316 94-95 20.19382394340646 30.973470537687625 28.279791569951612 20.5529139489543 96-97 20.35696480671255 30.84030166782579 28.16151494499377 20.641218580467886 98-99 20.37705697542377 30.85650017106244 28.137568940644282 20.628873912869512 100-101 20.540683238676902 30.76519083704245 27.989215743451084 20.70491018082956 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 122.0 1 190.5 2 250.5 3 219.5 4 205.5 5 220.5 6 279.5 7 388.5 8 485.5 9 604.0 10 774.0 11 873.5 12 905.0 13 962.5 14 963.0 15 925.5 16 979.0 17 1113.5 18 1379.5 19 1854.0 20 2589.0 21 3761.5 22 5465.0 23 7803.5 24 10913.5 25 14998.5 26 20175.0 27 26261.0 28 33354.5 29 41057.0 30 49390.0 31 58519.5 32 68367.0 33 79432.0 34 91353.0 35 104172.5 36 117551.0 37 130610.5 38 141189.0 39 147968.0 40 151782.0 41 151981.0 42 150183.0 43 147893.5 44 144346.5 45 139568.0 46 134874.0 47 129135.0 48 121149.5 49 112229.0 50 102922.5 51 93834.0 52 84063.0 53 73255.5 54 62787.0 55 52821.5 56 43473.5 57 35581.0 58 28994.0 59 23389.5 60 18533.0 61 14325.0 62 10906.0 63 8240.5 64 6218.0 65 4618.0 66 3388.5 67 2544.0 68 1919.5 69 1447.0 70 1084.0 71 807.0 72 621.5 73 460.0 74 350.5 75 296.5 76 254.5 77 220.5 78 185.0 79 144.0 80 124.5 81 106.0 82 76.0 83 76.0 84 77.0 85 57.0 86 45.0 87 43.5 88 36.5 89 27.0 90 22.0 91 17.5 92 15.0 93 11.5 94 9.5 95 8.5 96 8.5 97 6.5 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21989615997468856 2 0.20215710610090332 3 0.17872174173361638 4 0.16200430876355698 5 0.17373747005180237 6 0.13312029957639882 7 0.11962252036353604 8 0.11658861586156231 9 0.10414341576162921 10-11 0.08875718578733383 12-13 0.11682080243059093 14-15 0.1122389874684265 16-17 0.10336946053153388 18-19 0.09651221719288915 20-21 0.10093924110903449 22-23 0.08745694100077364 24-25 0.4057228107205802 26-27 0.19608929709695583 28-29 0.09052180371195119 30-31 0.1742792387128691 32-33 0.2039062449209188 34-35 0.203209685213833 36-37 0.20803916584962792 38-39 0.20930845242698431 40-41 0.206537692703243 42-43 0.22207871372355742 44-45 0.20477307477862558 46-47 0.22277527343064324 48-49 0.21285316738082097 50-51 0.2159025509873966 52-53 0.20742000166555163 54-55 0.19102762989213232 56-57 0.19793131054458277 58-59 0.1928541642351573 60-61 0.17390774020242333 62-63 0.16307236698108854 64-65 0.1672517252236034 66-67 0.17838120143237443 68-69 0.17184901929036975 70-71 0.16561094013580127 72-73 0.16872224016078458 74-75 0.1590632788891947 76-77 0.1692175715080456 78-79 0.1672672043282053 80-81 0.17251462078825172 82-83 0.196894210536255 84-85 0.1946497403689785 86-87 0.19847307920564952 88-89 0.20528388523048852 90-91 0.18732812389227657 92-93 0.16875319836998837 94-95 0.16971290285530657 96-97 0.17811805665414202 98-99 0.1916932313900143 100-101 0.20348830909666735 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3230161.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 93.82671972259101 #Duplication Level Percentage of deduplicated Percentage of total 1 97.89118553686575 91.84808828679655 2 1.4836348545536193 2.78409183337739 3 0.23980858553960352 0.675013588274861 4 0.08337043697073078 0.31289498491210777 5 0.06945044261623834 0.3258153606981842 6 0.04903232582765095 0.27603253756666596 7 0.013389064362734487 0.08793763925170141 8 0.01675711242756265 0.1257811912880694 9 0.019387808896830092 0.16371850602582302 >10 0.12169486386695745 2.312692903235919 >50 0.008903750933925512 0.5559190981324122 >100 0.003305632358384819 0.4786700179481626 >500 7.958478002704513E-5 0.053344052492069194 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.002321865690286026 0.0 0.0 2 0.002507614945508908 0.0 0.0 3 0.0029410298743622995 0.0 6.191641840762736E-5 4 0.0032196537571966225 0.0 6.191641840762736E-5 5 0.003931692568884338 0.0 6.191641840762736E-5 6 0.004488940334552984 0.0 6.191641840762736E-5 7 0.0054796030290750215 0.0 6.191641840762736E-5 8 0.00622260004996655 0.0 6.191641840762736E-5 9 0.007306137372100029 0.0 9.287462761144104E-5 10-11 0.01063414486151 0.0 9.287462761144104E-5 12-13 0.013435862794455137 0.0 9.287462761144104E-5 14-15 0.01645428819182697 0.0 9.287462761144104E-5 16-17 0.019426276275393083 0.0 9.287462761144104E-5 18-19 0.02301742854303547 0.0 9.287462761144104E-5 20-21 0.026608580810677858 0.0 9.287462761144104E-5 22-23 0.031097521145230837 0.0 1.2383283681525472E-4 24-25 0.035679336107395265 0.0 1.547910460190684E-4 26-27 0.04041594211557876 0.0 1.8574925522288208E-4 28-29 0.04694812425758345 0.0 2.4766567363050944E-4 30-31 0.0789743916789287 3.095820920381368E-5 2.786238828343231E-4 32-33 0.0871164006995317 3.095820920381368E-5 2.9410298743623E-4 34-35 0.09637290525147199 3.095820920381368E-5 3.095820920381368E-4 36-37 0.10525791129296652 3.095820920381368E-5 3.095820920381368E-4 38-39 0.11573726510845744 3.095820920381368E-5 3.095820920381368E-4 40-41 0.12759425923351808 3.095820920381368E-5 3.095820920381368E-4 42-43 0.14106108023717703 3.095820920381368E-5 3.095820920381368E-4 44-45 0.15508514900650464 3.095820920381368E-5 3.4054030124195045E-4 46-47 0.1704404207715962 3.095820920381368E-5 3.4054030124195045E-4 48-49 0.1871888119508594 3.095820920381368E-5 3.4054030124195045E-4 50-51 0.20571730015934192 3.095820920381368E-5 3.560194058438573E-4 52-53 0.22590205256022844 3.095820920381368E-5 4.024567196495778E-4 54-55 0.24926002140450584 3.095820920381368E-5 4.334149288533915E-4 56-57 0.27046639470911826 3.095820920381368E-5 4.334149288533915E-4 58-59 0.29656416506793315 3.095820920381368E-5 4.334149288533915E-4 60-61 0.32328109961082435 6.191641840762736E-5 4.79852242659112E-4 62-63 0.3532176879109122 6.191641840762736E-5 4.953313472610189E-4 64-65 0.3847331448803945 6.191641840762736E-5 4.953313472610189E-4 66-67 0.41959208844388873 6.191641840762736E-5 5.108104518629258E-4 68-69 0.45606085888598125 6.191641840762736E-5 5.262895564648325E-4 70-71 0.49568736666686275 6.191641840762736E-5 5.262895564648325E-4 72-73 0.5423723461462138 6.191641840762736E-5 5.262895564648325E-4 74-75 0.5916578151986851 6.191641840762736E-5 5.262895564648325E-4 76-77 0.646856302209085 6.191641840762736E-5 5.727268702705531E-4 78-79 0.7073022056795312 6.191641840762736E-5 5.8820597487246E-4 80-81 0.7768807808651024 6.191641840762736E-5 6.036850794743668E-4 82-83 0.8518460844521372 6.191641840762736E-5 6.191641840762736E-4 84-85 0.9327244059971005 6.191641840762736E-5 6.191641840762736E-4 86-87 1.024190435089768 6.191641840762736E-5 6.501223932800873E-4 88-89 1.125547612023054 6.191641840762736E-5 6.810806024839009E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGGA 2785 0.0 86.29375 2 GATCGGG 3140 0.0 78.212395 1 GAGCGGC 1860 0.0 77.82836 9 AAGCGGC 740 0.0 75.04213 9 AGAGCGG 2035 0.0 72.30167 8 TCGGGAG 2920 0.0 67.80879 3 CGGGAGA 2965 0.0 67.42022 4 GAGAGCG 2630 0.0 65.87109 7 GTTCGGA 400 0.0 61.744373 2 GATCCGG 610 0.0 60.740646 1 AAAGCGG 990 0.0 60.406876 8 ATCCGGA 610 0.0 59.95355 2 ATTCGGA 610 0.0 59.17493 2 TTCGGAA 525 0.0 58.787754 3 GGTTCGG 510 0.0 57.7479 1 TCGGAGA 725 0.0 57.633827 4 TCGGGAA 1085 0.0 56.016117 3 CGGAAAG 1210 0.0 55.723164 5 TTCGGAG 585 0.0 51.946575 3 GGATCGG 825 0.0 49.51755 1 >>END_MODULE