FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n02_52150223-1.3420000003573b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n02_52150223-1.3420000003573b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3230161
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA27850.086.293752
GATCGGG31400.078.2123951
GAGCGGC18600.077.828369
AAGCGGC7400.075.042139
AGAGCGG20350.072.301678
TCGGGAG29200.067.808793
CGGGAGA29650.067.420224
GAGAGCG26300.065.871097
GTTCGGA4000.061.7443732
GATCCGG6100.060.7406461
AAAGCGG9900.060.4068768
ATCCGGA6100.059.953552
ATTCGGA6100.059.174932
TTCGGAA5250.058.7877543
GGTTCGG5100.057.74791
TCGGAGA7250.057.6338274
TCGGGAA10850.056.0161173
CGGAAAG12100.055.7231645
TTCGGAG5850.051.9465753
GGATCGG8250.049.517551