##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l07n02_50330665-2.34200000035755.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2525090 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.06774055578217 33.0 33.0 33.0 33.0 33.0 2 32.01616655247932 33.0 33.0 33.0 33.0 33.0 3 32.00252228633435 33.0 33.0 33.0 33.0 33.0 4 35.74865846365872 37.0 37.0 37.0 33.0 37.0 5 35.70421925555129 37.0 37.0 37.0 33.0 37.0 6 35.78215984380755 37.0 37.0 37.0 33.0 37.0 7 35.699061419592965 37.0 37.0 37.0 33.0 37.0 8 35.64979822501376 37.0 37.0 37.0 33.0 37.0 9 35.61026141642476 37.0 37.0 37.0 33.0 37.0 10-11 35.548827368529444 37.0 37.0 37.0 33.0 37.0 12-13 35.54848401443117 37.0 37.0 37.0 33.0 37.0 14-15 37.281510956045125 40.0 37.0 40.0 33.0 40.0 16-17 37.254788542190575 40.0 37.0 40.0 33.0 40.0 18-19 37.26349298440847 40.0 37.0 40.0 33.0 40.0 20-21 37.24450970064433 40.0 37.0 40.0 33.0 40.0 22-23 37.29455484754999 40.0 37.0 40.0 33.0 40.0 24-25 37.09698961225145 40.0 37.0 40.0 33.0 40.0 26-27 37.01813578921939 40.0 37.0 40.0 33.0 40.0 28-29 36.97799920002851 40.0 37.0 40.0 33.0 40.0 30-31 36.973084325707205 40.0 37.0 40.0 33.0 40.0 32-33 36.903766796431015 40.0 37.0 40.0 33.0 40.0 34-35 36.85459369765038 40.0 37.0 40.0 33.0 40.0 36-37 36.77897639292065 40.0 37.0 40.0 33.0 40.0 38-39 36.735286267024144 40.0 37.0 40.0 33.0 40.0 40-41 36.654034113635554 40.0 37.0 40.0 33.0 40.0 42-43 36.55313751193027 40.0 37.0 40.0 33.0 40.0 44-45 36.45137856472442 40.0 37.0 40.0 33.0 40.0 46-47 36.366443770321055 38.5 37.0 40.0 33.0 40.0 48-49 36.26570993509142 37.0 37.0 40.0 33.0 40.0 50-51 35.133910474478135 37.0 35.0 38.5 30.0 38.5 52-53 35.554029163316955 37.0 37.0 38.5 33.0 40.0 54-55 36.35434222146537 37.0 37.0 40.0 33.0 40.0 56-57 36.40347571769719 37.0 37.0 40.0 33.0 40.0 58-59 36.20726469155555 37.0 37.0 40.0 33.0 40.0 60-61 36.007858531775106 37.0 37.0 40.0 33.0 40.0 62-63 35.82407914173356 37.0 37.0 40.0 33.0 40.0 64-65 35.599879806264326 37.0 37.0 40.0 27.0 40.0 66-67 35.34214622053075 37.0 37.0 40.0 27.0 40.0 68-69 35.13214895310662 37.0 33.0 38.5 27.0 40.0 70-71 34.84381863616743 37.0 33.0 37.0 27.0 40.0 72-73 34.623512231247204 37.0 33.0 37.0 27.0 40.0 74-75 34.37829760523387 37.0 33.0 37.0 27.0 40.0 76-77 34.13128581555509 37.0 33.0 37.0 27.0 37.0 78-79 33.877982962983495 37.0 33.0 37.0 27.0 37.0 80-81 33.66112990032038 37.0 33.0 37.0 27.0 37.0 82-83 33.44532967141765 37.0 33.0 37.0 27.0 37.0 84-85 33.26947692953519 37.0 33.0 37.0 27.0 37.0 86-87 33.09996831796094 37.0 33.0 37.0 27.0 37.0 88-89 32.931025626809344 37.0 33.0 37.0 27.0 37.0 90-91 32.79609142644421 37.0 33.0 37.0 27.0 37.0 92-93 32.61127623173827 33.0 33.0 37.0 27.0 37.0 94-95 32.48088186955712 33.0 33.0 37.0 24.5 37.0 96-97 32.349532689923926 33.0 33.0 37.0 22.0 37.0 98-99 32.207734773810046 33.0 33.0 37.0 22.0 37.0 100-101 30.965661817994608 33.0 30.0 37.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.10852001538800948 2306 2 0.07665144582014705 2306 3 0.13165236718145223 2306 4 0.1416898500700654 2306 5 0.31454583588728724 2306 6 0.14707199492838186 2306 7 0.1959928052197597 2306 8 0.08306436620314628 2306 9 0.1465949809319227 2306 10-11 0.055422329019130245 2306 12-13 0.13642546690741142 2306 14-15 0.27574614679930676 2306 16-17 0.2627406856412122 2306 18-19 0.41253076295522817 2306 20-21 0.2722186412450682 2306 22-23 0.3079345687028763 2306 24-25 0.25630522602197203 2306 26-27 0.345963457931056 2306 28-29 0.2603803001068883 2306 30-31 0.27626017233949796 2306 32-33 0.2634270953481419 2306 34-35 0.2535111364680347 2306 36-37 0.17043216610384349 2306 38-39 0.21505284215617593 2306 40-41 0.09694960061494129 2306 42-43 0.3297648388206369 2306 44-45 0.34089979678896043 2306 46-47 0.2820209583249067 2306 48-49 0.20536957440996417 2306 50-51 0.24317421663358374 2306 52-53 0.3466126614923155 2306 54-55 0.3161906167119284 2306 56-57 0.22957001572653013 2306 58-59 0.3962792345833961 2306 60-61 0.3955936784812337 2306 62-63 0.43583892719978934 2306 64-65 0.26924621018216754 2306 66-67 0.19200391896188762 2306 68-69 0.3134019540494606 2306 70-71 0.4438606921861705 2306 72-73 0.29193765923157855 2306 74-75 0.1886216049530418 2306 76-77 0.22150712634256564 2306 78-79 -0.08391650326228728 2306 80-81 0.05869792327701617 2306 82-83 0.2739789854633585 2306 84-85 0.13205236056191438 2306 86-87 0.1633445929604065 2306 88-89 0.2136795955345363 2306 90-91 0.114654813479099 2306 92-93 0.1860080681001861 2306 94-95 0.008028443938222551 2306 96-97 0.05581049426300666 2306 98-99 0.21931561640793262 2306 100-101 0.07663756823523116 2307 1 0.21606254148036896 2307 2 0.17942597646872827 2307 3 0.13541351992361683 2307 4 0.18017481203977326 2307 5 0.28230054675054106 2307 6 0.2318495861873302 2307 7 0.2941106135930056 2307 8 0.34298233514476806 2307 9 0.25197306442508705 2307 10-11 0.2897465718530725 2307 12-13 0.20161419457649998 2307 14-15 0.4614099151788267 2307 16-17 0.4814890778635714 2307 18-19 0.5197203257713241 2307 20-21 0.40352397122983774 2307 22-23 0.399375089604888 2307 24-25 0.3902431236291264 2307 26-27 0.4484463451945828 2307 28-29 0.4176000143621792 2307 30-31 0.39975663851902965 2307 32-33 0.4759331226447543 2307 34-35 0.3855881997178656 2307 36-37 0.46782466485650787 2307 38-39 0.34327602502345655 2307 40-41 0.4377072273138012 2307 42-43 0.42719706089795295 2307 44-45 0.4877648248286022 2307 46-47 0.41458977719013745 2307 48-49 0.2839741969127445 2307 50-51 0.19370880643641186 2307 52-53 0.34857639361831616 2307 54-55 0.4914249185206572 2307 56-57 0.36039166637424813 2307 58-59 0.2582601243140701 2307 60-61 0.36407196406082676 2307 62-63 0.3374501148195108 2307 64-65 0.3670421639068948 2307 66-67 0.3364816589440096 2307 68-69 0.2184946158831238 2307 70-71 0.11543703814282225 2307 72-73 0.19128509594047216 2307 74-75 0.12859272178867798 2307 76-77 0.21080336542911482 2307 78-79 0.24275609398507925 2307 80-81 0.08971771195371758 2307 82-83 0.13652730852885497 2307 84-85 0.3576799472143932 2307 86-87 0.30262988420296466 2307 88-89 0.21027080782578622 2307 90-91 0.23901454407837974 2307 92-93 0.2692861636778545 2307 94-95 0.14079199084824268 2307 96-97 0.256347177915778 2307 98-99 0.11468623383044019 2307 100-101 -0.13061618603771663 2201 1 0.34080639462052886 2201 2 0.2818743495472589 2201 3 0.3271233231485766 2201 4 0.4875369501168194 2201 5 0.45855697601678 2201 6 0.3880700525142231 2201 7 0.38503749692725364 2201 8 0.3752936693208895 2201 9 0.41849019575045077 2201 10-11 0.3615741933263834 2201 12-13 0.3153064375481165 2201 14-15 0.5896254790157016 2201 16-17 0.5771834971195489 2201 18-19 0.5221034954135391 2201 20-21 0.6078872830751081 2201 22-23 0.49963787115369485 2201 24-25 0.6445366582244461 2201 26-27 0.548501310734089 2201 28-29 0.5812188901719679 2201 30-31 0.5534801217014902 2201 32-33 0.6147621944424699 2201 34-35 0.6184903802037383 2201 36-37 0.5547780475905739 2201 38-39 0.5853650559014838 2201 40-41 0.5260933837626922 2201 42-43 0.5208363220887691 2201 44-45 0.5173260205936785 2201 46-47 0.5427549493011483 2201 48-49 0.5357079913857703 2201 50-51 0.47966935250720866 2201 52-53 0.519473217423851 2201 54-55 0.6214039268434064 2201 56-57 0.5925268023574475 2201 58-59 0.6708190454046203 2201 60-61 0.6950704807624319 2201 62-63 0.7478408621008938 2201 64-65 0.8589486411183955 2201 66-67 0.8160020094896012 2201 68-69 0.7091819856158637 2201 70-71 0.7620739023361907 2201 72-73 0.738429812999577 2201 74-75 0.6721329014119064 2201 76-77 0.55271561309155 2201 78-79 0.4864519428237273 2201 80-81 0.5791970098626891 2201 82-83 0.5961131443853134 2201 84-85 0.6414243210247434 2201 86-87 0.7181890408980962 2201 88-89 0.7223863615045474 2201 90-91 0.6097414187968369 2201 92-93 0.6457272473738556 2201 94-95 0.6376595933029918 2201 96-97 0.5432938860729664 2201 98-99 0.635652382822947 2201 100-101 0.548200648149308 1101 1 0.21273988106575104 1101 2 0.27250037019913265 1101 3 0.11207239668969038 1101 4 0.4095931361722478 1101 5 0.44152618677517097 1101 6 0.36820214407506313 1101 7 0.40225398946343915 1101 8 0.34691700782335033 1101 9 0.3737117400413368 1101 10-11 0.2802946220952407 1101 12-13 0.3291823544092978 1101 14-15 0.4887202210339723 1101 16-17 0.3524774318363768 1101 18-19 0.3998807754358822 1101 20-21 0.43685949738245 1101 22-23 0.40242948913727616 1101 24-25 0.4826089850997448 1101 26-27 0.5200040592991257 1101 28-29 0.4334710830596151 1101 30-31 0.5196263850542877 1101 32-33 0.3626421035338012 1101 34-35 0.2949249186203602 1101 36-37 0.3009677441571128 1101 38-39 0.45001365994924214 1101 40-41 0.4411811383363826 1101 42-43 0.21607520736893093 1101 44-45 0.4263028865992453 1101 46-47 0.3671545129895648 1101 48-49 0.39606276020990805 1101 50-51 0.3572206891802594 1101 52-53 0.29348962807347334 1101 54-55 0.30681694607684307 1101 56-57 0.4498411680830259 1101 58-59 0.41796890353563754 1101 60-61 0.4388016271898678 1101 62-63 0.5250990063493717 1101 64-65 0.4959346427884199 1101 66-67 0.5923931042743291 1101 68-69 0.4214524414979124 1101 70-71 0.5091116352662013 1101 72-73 0.44482209545193996 1101 74-75 0.3508718015379202 1101 76-77 0.3133383364030209 1101 78-79 0.417998661136572 1101 80-81 0.41865273512390644 1101 82-83 0.28333449177341663 1101 84-85 0.25255888741405386 1101 86-87 0.2924208725785107 1101 88-89 0.2994714604950772 1101 90-91 0.42559524504002155 1101 92-93 0.6215566482945079 1101 94-95 0.41326281646626484 1101 96-97 0.2371906548356364 1101 98-99 0.20101447299297348 1101 100-101 0.4604717291195861 2304 1 0.29479700764171923 2304 2 0.12581206313615922 2304 3 0.13469814333424068 2304 4 0.20506086382887645 2304 5 0.29465810839487716 2304 6 0.35588950479469617 2304 7 0.23312649089652382 2304 8 0.25895625902494146 2304 9 0.1736991899682394 2304 10-11 0.20736088987309387 2304 12-13 0.28168541560955873 2304 14-15 0.47527553124125177 2304 16-17 0.3101997970842021 2304 18-19 0.4101650515510542 2304 20-21 0.45983291497086753 2304 22-23 0.3620157098354042 2304 24-25 0.3392642549432807 2304 26-27 0.390590758256792 2304 28-29 0.34449530470068623 2304 30-31 0.4165801092036787 2304 32-33 0.33658459027990517 2304 34-35 0.2935902013870759 2304 36-37 0.3341168966879948 2304 38-39 0.293098739269098 2304 40-41 0.3609268128010612 2304 42-43 0.3881969159426646 2304 44-45 0.4259713272357075 2304 46-47 0.37983093479413554 2304 48-49 0.3881613020270933 2304 50-51 0.2874940094208043 2304 52-53 0.2592287765001373 2304 54-55 0.4122095768096017 2304 56-57 0.43715117863187203 2304 58-59 0.5486957223634619 2304 60-61 0.46663141427957555 2304 62-63 0.5144941945835129 2304 64-65 0.5014990738461265 2304 66-67 0.48992684531544484 2304 68-69 0.583305137558888 2304 70-71 0.5773891207742636 2304 72-73 0.5498110886986467 2304 74-75 0.5427174763417781 2304 76-77 0.6034790668172718 2304 78-79 0.5732574809548368 2304 80-81 0.4630749060152013 2304 82-83 0.5953929072758086 2304 84-85 0.48089305176574726 2304 86-87 0.4698110679186982 2304 88-89 0.36951952388312037 2304 90-91 0.4721416006005583 2304 92-93 0.44610662641667176 2304 94-95 0.566494121097243 2304 96-97 0.49022980767178126 2304 98-99 0.608249081345889 2304 100-101 0.40816590758162974 2202 1 0.11675631013147125 2202 2 0.14760412437495063 2202 3 0.16270816497420526 2202 4 0.2631094934260858 2202 5 0.2994990773245405 2202 6 0.28659027515692514 2202 7 0.29521325440823887 2202 8 0.2930702226639781 2202 9 0.3273273282552367 2202 10-11 0.1732848967150531 2202 12-13 0.28439319222077586 2202 14-15 0.4165989447596843 2202 16-17 0.32526306125350146 2202 18-19 0.3848075086668743 2202 20-21 0.4085096597518998 2202 22-23 0.41950066496819716 2202 24-25 0.44863357274101645 2202 26-27 0.3589260510808572 2202 28-29 0.3837157097590804 2202 30-31 0.3563744354084051 2202 32-33 0.35168892674414565 2202 34-35 0.2914938029995042 2202 36-37 0.31314382128866924 2202 38-39 0.21423800772018353 2202 40-41 0.26154512197447133 2202 42-43 0.2951615364389468 2202 44-45 0.2349146697161828 2202 46-47 0.4107888562279598 2202 48-49 0.28007281946869966 2202 50-51 0.21240037742343532 2202 52-53 0.33028855294706005 2202 54-55 0.3515583113978664 2202 56-57 0.2877678192432995 2202 58-59 0.42478007373352256 2202 60-61 0.45484248908164204 2202 62-63 0.48090625580518775 2202 64-65 0.4451930219290361 2202 66-67 0.5135471584745801 2202 68-69 0.4246977322241108 2202 70-71 0.5278468415528224 2202 72-73 0.4234100314293272 2202 74-75 0.29954799151670386 2202 76-77 0.4489028430554072 2202 78-79 0.4494322127655863 2202 80-81 0.4618485129584755 2202 82-83 0.3965960474238841 2202 84-85 0.42262387250143263 2202 86-87 0.5360431383645263 2202 88-89 0.6006085129998198 2202 90-91 0.35690974749561377 2202 92-93 0.3962198319296064 2202 94-95 0.27819003466059655 2202 96-97 0.36909648614673785 2202 98-99 0.4632392796138376 2202 100-101 0.4443226830984308 1102 1 -0.021595917102438023 1102 2 -0.08800109207642492 1102 3 -0.026051243053977657 1102 4 -0.06119724832687723 1102 5 0.006109551595919527 1102 6 -0.05270509022152936 1102 7 -0.032857532663399525 1102 8 1.2107272269190616E-4 1102 9 2.949272730603525E-4 1102 10-11 0.0179606867225246 1102 12-13 0.05900002338336208 1102 14-15 -0.14946576379161058 1102 16-17 -0.055451398418753683 1102 18-19 -0.03928813628348138 1102 20-21 -0.06482216674003638 1102 22-23 0.03299000920861772 1102 24-25 -0.01817977464875753 1102 26-27 0.021089430497966077 1102 28-29 -0.06592506703711365 1102 30-31 0.016851792753335815 1102 32-33 0.08358055760248106 1102 34-35 -0.09719329332268956 1102 36-37 -0.08542629187456185 1102 38-39 -0.049507888778158815 1102 40-41 -0.04999656026103594 1102 42-43 -0.04939581917117408 1102 44-45 -0.025658102382678294 1102 46-47 0.06063255618526142 1102 48-49 -0.08828484573894713 1102 50-51 0.019563740159632914 1102 52-53 0.032989441038232314 1102 54-55 0.13915330912926294 1102 56-57 0.21027168921234107 1102 58-59 0.1727153952078453 1102 60-61 0.1856994870532418 1102 62-63 0.13724883480593775 1102 64-65 0.21817596737580658 1102 66-67 0.27830141544052367 1102 68-69 0.21445165557203438 1102 70-71 0.2543330264305794 1102 72-73 0.28082335746753984 1102 74-75 0.3043725251187084 1102 76-77 0.3583668356624372 1102 78-79 0.13724080943474348 1102 80-81 0.2743918833011705 1102 82-83 0.26991919984728696 1102 84-85 0.23862310851616542 1102 86-87 0.1241599980848278 1102 88-89 0.10741932489263917 1102 90-91 0.10049468997986821 1102 92-93 0.1311882549839467 1102 94-95 0.23634140716513485 1102 96-97 0.08297608006687796 1102 98-99 0.13891168673458054 1102 100-101 0.436013092247574 2305 1 0.1575238166815751 2305 2 0.12636807541608164 2305 3 0.12248119188149076 2305 4 0.13214042067574638 2305 5 0.24453246664040051 2305 6 0.20564420692813457 2305 7 0.20995893432652935 2305 8 0.21714583765273687 2305 9 0.09524755934875628 2305 10-11 0.19411900353902212 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-0.30818856330621713 1213 70-71 -0.4604572864969114 1213 72-73 -0.4867253771425766 1213 74-75 -0.4679609649633676 1213 76-77 -0.36355839518572 1213 78-79 -0.27519506607824695 1213 80-81 -0.3039511399546484 1213 82-83 -0.27337231007063423 1213 84-85 -0.3563570128928575 1213 86-87 -0.32403169138165566 1213 88-89 -0.4562049911128341 1213 90-91 -0.4099971448354509 1213 92-93 -0.2250735011720053 1213 94-95 -0.3092649967336385 1213 96-97 -0.2763187643243654 1213 98-99 -0.33636832352533474 1213 100-101 -0.0666309740998976 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 368.0 3 169.0 4 94.0 5 128.0 6 337.0 7 1618.0 8 6290.0 9 10261.0 10 10020.0 11 9048.0 12 6896.0 13 5262.0 14 4400.0 15 4087.0 16 3998.0 17 4376.0 18 4877.0 19 5564.0 20 6369.0 21 7314.0 22 8586.0 23 9440.0 24 10841.0 25 12615.0 26 15076.0 27 18199.0 28 21898.0 29 27532.0 30 34972.0 31 45285.0 32 61933.0 33 90763.0 34 140696.0 35 240379.0 36 481701.0 37 884317.0 38 328638.0 39 743.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.96054772772376 24.77035126017067 26.218535423695176 13.050565588410398 2 31.571714410662654 22.281735213091956 35.16443919555474 10.982111180690648 3 39.950121264996454 18.404110591772387 26.859175096498213 14.786593046732946 4 30.623566494427823 17.864880967964844 36.957846308368616 14.553706229238713 5 30.495567048099947 16.755955167439744 38.14953020389833 14.598947580561978 6 23.454044056413277 17.394066485940304 44.57908153148937 14.572807926157047 7 34.01018549431447 17.206340319537766 32.594295520491926 16.189178665655838 8 29.98470338119507 28.160924553333178 26.568458514760962 15.28591355071079 9 30.781493069376015 27.695418858338076 24.871155886312714 16.651932185973198 10-11 24.745069911421815 28.823138739325106 30.5770434788813 15.854747870371783 12-13 25.835700071408546 26.33431095508636 29.916415124338496 17.91357384916659 14-15 23.786847258932063 27.240095479398203 31.199108562914528 17.773948698755206 16-17 21.962878307792852 28.862386263106636 32.51489087819362 16.65984455090689 18-19 21.10779816922907 29.30906365004181 32.33061067783874 17.252527502890374 20-21 21.155130367975033 29.49132297414325 31.781873062557718 17.571673595323993 22-23 21.22452668346258 29.01554044733784 32.04314509544021 17.716787773759375 24-25 20.838716347463375 29.620992065148886 31.53744450475276 18.002847082634982 26-27 20.524600272479944 29.934363958526067 31.17089991986701 18.370135849126978 28-29 20.26864751681896 29.770187954166417 30.898547162949434 19.06261736606519 30-31 20.182015311486154 30.06860732758313 30.638523407182543 19.110853953748173 32-33 19.625928828659013 30.122659947023283 31.89434418992252 18.357067034395183 34-35 19.895777653703554 30.132992221816135 31.683509940686438 18.28772018379387 36-37 20.07405601009108 30.60337532062927 31.545852242366063 17.77671642691359 38-39 20.770982255800224 30.055787327555844 30.770119104902598 18.403111311741334 40-41 20.20664794133824 29.649400104288283 30.708195995298297 19.435755959075184 42-43 20.40787814815454 30.17884333779246 31.17898165606061 18.234296857992387 44-45 19.939702081855565 30.46417888977624 31.41281454769121 18.183304480676988 46-47 20.356140678147735 30.02431028255494 31.130815118812784 18.48873392048454 48-49 20.15310490954452 30.45614579552821 30.531033419445503 18.859715875481765 50-51 19.91235454104204 30.129733852919614 30.41566108940877 19.54225051662958 52-53 20.416323566469504 29.915056658657136 30.30312530382969 19.365494471043665 54-55 20.881433942051125 29.93032644599407 30.039022745082477 19.14921686687233 56-57 20.969507706897815 30.27028206710328 29.821637247063165 18.938572978935735 58-59 20.780140085170036 30.11771637960879 30.42488798245414 18.677255552767033 60-61 20.18709974567002 30.628890473079146 29.994910226794424 19.18909955445641 62-63 20.4523791107118 30.482248151035 30.008270511165193 19.05710222708801 64-65 20.160383705450293 30.34076826774015 30.252228179549146 19.246619847260416 66-67 20.448847432458354 29.51988039410278 30.26239862826937 19.768873545169498 68-69 20.330465697864465 29.704007813466966 30.905304557009426 19.06022193165914 70-71 19.648127983752126 30.830561555289783 30.574328277689517 18.946982183268577 72-73 19.51363190900279 31.356494795345334 30.10127263679164 19.02860065886023 74-75 20.06828355026863 30.628573581822256 29.883305138024795 19.419837729884318 76-77 20.071924024175136 30.363930167330167 30.14319152493728 19.42095428355742 78-79 19.87728219086356 30.544223097613138 30.46105853630769 19.117436175215612 80-81 19.18339448181939 30.882726017161776 30.31649176354984 19.61738773746899 82-83 18.674929583277038 30.745001687382484 30.258051473002574 20.3220172563379 84-85 18.68055169786765 30.370582726904793 30.821158470292275 20.127707104935283 86-87 18.82567007505031 30.31094482204047 31.44664944122468 19.41673566168454 88-89 19.001354984782463 30.84339419620473 30.812719039173125 19.342531779839675 90-91 19.26975711868872 30.486393472802813 30.764622235765003 19.47922717274346 92-93 18.70288942543322 31.57109396399529 30.5628089094039 19.163207701167593 94-95 18.456321326798196 31.81611810846047 30.525315912125166 19.202244652616166 96-97 19.00839807056823 31.772644113783077 30.2910552227144 18.92790259293429 98-99 19.33811506387963 30.90403989704347 29.95863169551722 19.799213343559682 100-101 19.174521352742122 30.907770558840948 29.576437231460012 20.341270856956918 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 181.0 1 182.5 2 207.0 3 262.5 4 276.5 5 282.5 6 334.0 7 411.5 8 528.0 9 660.5 10 762.5 11 837.0 12 859.0 13 833.0 14 807.0 15 752.5 16 753.5 17 827.5 18 923.5 19 1151.0 20 1607.0 21 2276.5 22 3237.0 23 4640.0 24 6557.0 25 9197.5 26 13103.0 27 18619.0 28 24463.5 29 30287.5 30 36804.0 31 43786.0 32 52700.5 33 64890.5 34 80393.0 35 99961.5 36 127018.0 37 155939.5 38 181833.5 39 193569.5 40 185604.5 41 166649.0 42 147361.0 43 137652.0 44 124271.0 45 108074.5 46 101263.5 47 90729.5 48 70555.0 49 52617.5 50 40096.5 51 31876.5 52 26447.0 53 20630.5 54 15291.5 55 11342.5 56 8182.0 57 5902.5 58 4337.5 59 3222.5 60 2393.0 61 1763.0 62 1367.0 63 1038.5 64 752.5 65 551.0 66 459.5 67 405.5 68 327.0 69 253.5 70 208.5 71 172.5 72 136.5 73 114.5 74 91.5 75 62.5 76 47.0 77 44.5 78 40.5 79 29.5 80 18.5 81 17.5 82 11.0 83 6.0 84 9.0 85 9.0 86 7.5 87 5.0 88 1.0 89 1.5 90 4.0 91 4.0 92 2.0 93 1.0 94 0.5 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2213782479040351 2 0.20561643347365835 3 0.1812608659493325 4 0.16522183367721546 5 0.175122470882226 6 0.13520310167162358 7 0.1216590299751692 8 0.11738195470260467 9 0.10542198495895196 10-11 0.09070963807230634 12-13 0.11813440313018546 14-15 0.11367911638793073 16-17 0.10520417094044172 18-19 0.0986697503851348 20-21 0.10278841546241917 22-23 0.08926414504037479 24-25 0.4053320871731304 26-27 0.19423070068789627 28-29 0.08979877944944537 30-31 0.17298393324594372 32-33 0.20256703721451513 34-35 0.20169578114047423 36-37 0.2075371570914304 38-39 0.20801238767727093 40-41 0.20403233152085667 42-43 0.21933871663980292 44-45 0.20207200535426462 46-47 0.2209822224158347 48-49 0.2104677457041135 50-51 0.21357654578648683 52-53 0.20494319014371767 54-55 0.18870614512750042 56-57 0.19583460391510796 58-59 0.19157732991695345 60-61 0.17223148481836292 62-63 0.1617764119298718 64-65 0.16625149994653657 66-67 0.17736001489055836 68-69 0.17084539560966144 70-71 0.1641525648590743 72-73 0.16718215984380755 74-75 0.1578161570478676 76-77 0.16773659552728812 78-79 0.165716865537466 80-81 0.17082559433525143 82-83 0.1946267261760967 84-85 0.19286441275360483 86-87 0.19724049439821945 88-89 0.20337888946532598 90-91 0.18541913357543693 92-93 0.16734057003908773 94-95 0.16848904395486894 96-97 0.1762907460724172 98-99 0.190211041982662 100-101 0.2018739926101644 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2525090.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.38712657717802 #Duplication Level Percentage of deduplicated Percentage of total 1 92.18963452951397 57.514463984965694 2 3.976371101699928 4.961487344791725 3 1.160220377816044 2.1714844670469255 4 0.6152911230214914 1.5354498069502318 5 0.32124864128256303 1.002088982322086 6 0.2744037158323874 1.0271555611729883 7 0.17402801530205644 0.7599975473037128 8 0.17365625801102472 0.8667131959562308 9 0.08776066642849119 0.49276222244746 >10 0.8317423962182331 9.776166809041952 >50 0.09497390012612794 4.168606719006059 >100 0.09423891628411572 11.767542133338067 >500 0.005009366282989019 2.102774578339088 >1k 0.0012933927720862061 1.2178151068740883 >5k 1.2759940845563346E-4 0.6354915404436673 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 8016 0.3174540313414571 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 7950 0.31484026311933433 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 4015 0.15900423351246887 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 2.772178417402944E-4 0.0 0.0 2 3.564229393803785E-4 0.0 0.0 3 5.148331346605467E-4 0.0 0.0 4 5.940382323006308E-4 0.0 0.0 5 8.712560740409252E-4 0.0 0.0 6 9.504611716810094E-4 0.0 0.0 7 9.900637205010514E-4 0.0 0.0 8 0.001386089208701472 0.0 0.0 9 0.0015048968551615981 0.0 0.0 10-11 0.002019729989822145 0.0 1.9801274410021028E-5 12-13 0.002791979691812965 0.0 3.9602548820042056E-5 14-15 0.0037226395890839533 0.0 3.9602548820042056E-5 16-17 0.004633498211944921 0.0 3.9602548820042056E-5 18-19 0.005663164481266014 0.0 3.9602548820042056E-5 20-21 0.007049253689967486 0.0 3.9602548820042056E-5 22-23 0.008771964563639316 0.0 3.9602548820042056E-5 24-25 0.011049111120791735 0.0 3.9602548820042056E-5 26-27 0.013247052580304067 0.0 3.9602548820042056E-5 28-29 0.015187577472486127 0.0 3.9602548820042056E-5 30-31 0.02304868341326448 0.0 3.9602548820042056E-5 32-33 0.026256489867687884 0.0 3.9602548820042056E-5 34-35 0.028771251717760557 0.0 9.900637205010514E-5 36-37 0.03174144287926371 0.0 1.1880764646012617E-4 38-39 0.035701697761267914 0.0 1.5841019528016823E-4 40-41 0.03936493352712181 0.0 1.5841019528016823E-4 42-43 0.04409743811111683 0.0 1.7821146969018925E-4 44-45 0.049503186025052576 0.0 1.9801274410021028E-4 46-47 0.05409708168817745 0.0 1.9801274410021028E-4 48-49 0.05983945126708355 0.0 1.9801274410021028E-4 50-51 0.06566102594362974 0.0 2.178140185102313E-4 52-53 0.07201723502924648 0.0 2.3761529292025234E-4 54-55 0.07869026450542357 0.0 2.3761529292025234E-4 56-57 0.08562071054893093 0.0 2.3761529292025234E-4 58-59 0.09284817570858861 0.0 2.970191161503154E-4 60-61 0.10100630076551727 0.0 3.1682039056033645E-4 62-63 0.10934263729213611 0.0 3.1682039056033645E-4 64-65 0.12049075478497795 0.0 3.1682039056033645E-4 66-67 0.13229231433335048 0.0 3.1682039056033645E-4 68-69 0.14391566241203285 0.0 3.1682039056033645E-4 70-71 0.1585686054754484 0.0 3.1682039056033645E-4 72-73 0.17425121480818506 0.0 3.1682039056033645E-4 74-75 0.19242878471658437 0.0 3.1682039056033645E-4 76-77 0.21151721324784464 0.0 3.1682039056033645E-4 78-79 0.2347243068563893 0.0 3.1682039056033645E-4 80-81 0.2630203279883093 0.0 3.1682039056033645E-4 82-83 0.2945241555746528 0.0 3.1682039056033645E-4 84-85 0.3297110202012602 0.0 3.1682039056033645E-4 86-87 0.36957098558863244 0.0 3.1682039056033645E-4 88-89 0.41428226320645994 0.0 3.1682039056033645E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 560 0.0 58.473354 9 CGGGAGA 670 0.0 57.401394 4 AGAGCGG 655 0.0 48.545353 8 GTCGCTA 50 2.8349512E-5 47.480167 5 TTGTGTT 34575 0.0 46.88023 1 TCGGGAG 875 0.0 46.66622 3 GCGCAAC 60 8.310117E-5 39.569946 2 TCGGAGA 180 0.0 39.566807 4 GAGAGCG 935 0.0 38.57591 7 CGGAAAG 240 0.0 37.588467 5 AGCGGCG 425 0.0 36.848682 10-11 GATCGGG 1360 0.0 36.666206 1 GATCCGG 160 1.8189894E-12 35.618603 1 TGTGTTT 57440 0.0 35.221737 1 ATTCGGA 135 3.092282E-10 35.173286 2 TCGGGAA 260 0.0 34.69704 3 GTTCCGA 110 0.0 34.53994 30-31 ACGGCGT 55 0.0025854963 34.531033 4 CGGCGTG 165 0.0 34.51392 12-13 ACCGGCC 85 1.6349812E-5 33.523388 1 >>END_MODULE