FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n02_50330665-2.34200000035755.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n02_50330665-2.34200000035755.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2525090
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC80160.3174540313414571No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG79500.31484026311933433No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA40150.15900423351246887No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC5600.058.4733549
CGGGAGA6700.057.4013944
AGAGCGG6550.048.5453538
GTCGCTA502.8349512E-547.4801675
TTGTGTT345750.046.880231
TCGGGAG8750.046.666223
GCGCAAC608.310117E-539.5699462
TCGGAGA1800.039.5668074
GAGAGCG9350.038.575917
CGGAAAG2400.037.5884675
AGCGGCG4250.036.84868210-11
GATCGGG13600.036.6662061
GATCCGG1601.8189894E-1235.6186031
TGTGTTT574400.035.2217371
ATTCGGA1353.092282E-1035.1732862
TCGGGAA2600.034.697043
GTTCCGA1100.034.5399430-31
ACGGCGT550.002585496334.5310334
CGGCGTG1650.034.5139212-13
ACCGGCC851.6349812E-533.5233881
Produced by FastQC (version 0.11.2)